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The map label for this gene is thyA

Identifier: 119944280

GI number: 119944280

Start: 642363

End: 643214

Strand: Direct

Name: thyA

Synonym: Ping_0506

Alternate gene names: 119944280

Gene position: 642363-643214 (Clockwise)

Preceding gene: 119944279

Following gene: 119944286

Centisome position: 14.09

GC content: 41.43

Gene sequence:

>852_bases
ATGAAACAATATTTAGGATTATGTCAGCGCATTGTCGACAAGGGTATCTGGATTGAAAACCAGCGAACCGGCAAACGCTG
TTTGACGGTGATCAATGCTGACCTTGAGTATGATGTGGGAAATAATCAATTTCCGATGATAACGACGCGTAAAAGTTTTT
ATAAATCAGCCATTGCAGAATTGATTGGTTATCTCCGAGGTTATGATAATGCGGCTGATTTTAGAAAATTAGGCACCAAA
ACCTGGGATGCAAATTCCAATTTAAATAATGCCTGGCTGAATAATCCGTACCGTAAAGGTGAGGACGACATGGGCCGCGT
TTATGGGATTCAGGGTCGTTCCTGGGCTAAGCCCGACGGCGGTTTTATTGATCAGCTTAAAAAAGTAGTGGATGATCTCA
GTAACGGTATTGATGATCGCGGGGAAATAGTGACTTTTTATAATCCCGGCGAATTTGAGATGGGCTGTTTGCGGCCTTGT
ATGCATACTCACACCTTTTCATTACTTGGCGACACACTTCACTTGACCAGTTACCAACGCTCCTGTGATGTTCCGCTTGG
CCTTAATTTTAATCAAGTTCAGGTCTTTGCTTTGCTCGCCTTAATGGCGCAAATAACCGGTAAAAAAGCGGGTATGGCTT
ACCATAAAATAGTGAATGCGCATATCTATGAAGATCAATTAGCATTAATGCGTGATGTGCAGCTACCGCGTTTACCCTTT
GCATCCCCGCAATTAAAGATTAACCCGAAAATTAAATCATTACAAGATCTCGAAACCTGGGTAACCATGGATGATTTTGA
AGTAGAAGGTTATCAATGCCATGATGCAATTAAATATCCTTTTTCTGTTTAA

Upstream 100 bases:

>100_bases
GTCTCAAGCAGATAATGTTAAACCGCCTAAAGCAAAAACAAATAAGGCCAAAAAAAAACATGGCTAAAAAATCATAACTC
ACCCGTTTAGGTAACAGAAA

Downstream 100 bases:

>100_bases
TTTTTAAAGCGTATTAATAGAAAAGCCCCGCTTAATACAAGACATTAAGCGGGGCTTTTTGTTGCTTCTTTATTACTTAA
GTCATCGTTACAAATTCTTC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKQYLGLCQRIVDKGIWIENQRTGKRCLTVINADLEYDVGNNQFPMITTRKSFYKSAIAELIGYLRGYDNAADFRKLGTK
TWDANSNLNNAWLNNPYRKGEDDMGRVYGIQGRSWAKPDGGFIDQLKKVVDDLSNGIDDRGEIVTFYNPGEFEMGCLRPC
MHTHTFSLLGDTLHLTSYQRSCDVPLGLNFNQVQVFALLALMAQITGKKAGMAYHKIVNAHIYEDQLALMRDVQLPRLPF
ASPQLKINPKIKSLQDLETWVTMDDFEVEGYQCHDAIKYPFSV

Sequences:

>Translated_283_residues
MKQYLGLCQRIVDKGIWIENQRTGKRCLTVINADLEYDVGNNQFPMITTRKSFYKSAIAELIGYLRGYDNAADFRKLGTK
TWDANSNLNNAWLNNPYRKGEDDMGRVYGIQGRSWAKPDGGFIDQLKKVVDDLSNGIDDRGEIVTFYNPGEFEMGCLRPC
MHTHTFSLLGDTLHLTSYQRSCDVPLGLNFNQVQVFALLALMAQITGKKAGMAYHKIVNAHIYEDQLALMRDVQLPRLPF
ASPQLKINPKIKSLQDLETWVTMDDFEVEGYQCHDAIKYPFSV
>Mature_283_residues
MKQYLGLCQRIVDKGIWIENQRTGKRCLTVINADLEYDVGNNQFPMITTRKSFYKSAIAELIGYLRGYDNAADFRKLGTK
TWDANSNLNNAWLNNPYRKGEDDMGRVYGIQGRSWAKPDGGFIDQLKKVVDDLSNGIDDRGEIVTFYNPGEFEMGCLRPC
MHTHTFSLLGDTLHLTSYQRSCDVPLGLNFNQVQVFALLALMAQITGKKAGMAYHKIVNAHIYEDQLALMRDVQLPRLPF
ASPQLKINPKIKSLQDLETWVTMDDFEVEGYQCHDAIKYPFSV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=289, Percent_Identity=32.5259515570934, Blast_Score=152, Evalue=3e-37,
Organism=Escherichia coli, GI1789191, Length=283, Percent_Identity=34.2756183745583, Blast_Score=152, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI71993377, Length=291, Percent_Identity=26.8041237113402, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6324648, Length=226, Percent_Identity=30.5309734513274, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI17137556, Length=291, Percent_Identity=27.8350515463918, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_PSYIN (A1SS96)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_941960.1
- ProteinModelPortal:   A1SS96
- SMR:   A1SS96
- STRING:   A1SS96
- GeneID:   4623431
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0506
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- ProtClustDB:   PRK01827
- BioCyc:   PING357804:PING_0506-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 32251; Mature: 32251

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 160-160

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLGLCQRIVDKGIWIENQRTGKRCLTVINADLEYDVGNNQFPMITTRKSFYKSAIAE
CHHHHHHHHHHHHCCCEEECCCCHHHEEEEEECCEEECCCCCCCCEEECHHHHHHHHHHH
LIGYLRGYDNAADFRKLGTKTWDANSNLNNAWLNNPYRKGEDDMGRVYGIQGRSWAKPDG
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCEEECCCCCCCCCCC
GFIDQLKKVVDDLSNGIDDRGEIVTFYNPGEFEMGCLRPCMHTHTFSLLGDTLHLTSYQR
CHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHEHHHHH
SCDVPLGLNFNQVQVFALLALMAQITGKKAGMAYHKIVNAHIYEDQLALMRDVQLPRLPF
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ASPQLKINPKIKSLQDLETWVTMDDFEVEGYQCHDAIKYPFSV
CCCCEEECCCHHHHHHHHHHEECCCEECCCEEECCCCCCCCCC
>Mature Secondary Structure
MKQYLGLCQRIVDKGIWIENQRTGKRCLTVINADLEYDVGNNQFPMITTRKSFYKSAIAE
CHHHHHHHHHHHHCCCEEECCCCHHHEEEEEECCEEECCCCCCCCEEECHHHHHHHHHHH
LIGYLRGYDNAADFRKLGTKTWDANSNLNNAWLNNPYRKGEDDMGRVYGIQGRSWAKPDG
HHHHHHCCCCHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCCCCEEECCCCCCCCCCC
GFIDQLKKVVDDLSNGIDDRGEIVTFYNPGEFEMGCLRPCMHTHTFSLLGDTLHLTSYQR
CHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHEHHHHH
SCDVPLGLNFNQVQVFALLALMAQITGKKAGMAYHKIVNAHIYEDQLALMRDVQLPRLPF
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ASPQLKINPKIKSLQDLETWVTMDDFEVEGYQCHDAIKYPFSV
CCCCEEECCCHHHHHHHHHHEECCCEECCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA