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The map label for this gene is tnpR [H]

Identifier: 119944032

GI number: 119944032

Start: 314355

End: 314894

Strand: Direct

Name: tnpR [H]

Synonym: Ping_0246

Alternate gene names: 119944032

Gene position: 314355-314894 (Clockwise)

Preceding gene: 119944031

Following gene: 119944033

Centisome position: 6.89

GC content: 39.81

Gene sequence:

>540_bases
ATGAAAATTGGATATGCCCGTGTCAGTACGCAGGAACAGACGCTTGAGGTTCAGATTGAACAATTGAACAATATTAAGTG
TGAGGCAATTTATAAAGAACATGCGAGTGGTAAATCGTCCGATAGGAAGCAACTAAACTTACTCATAGATTTTGCTAGAA
AAGGGGATGTTGTGCACGTGATGAAGGTTGACCGTTTAGCTCGTAATACTATTGATGCACTTCAACTTGCTGATAAGTTA
GCAAATAAAGGTGTTGGTCTGGTATGTCATGATCTTGCAGACCTTGATATCAATAGCGATATTGGAAGAGTTATTTATAC
CACCATTTCTGCTTTTGCTGAAATGGAACGGAAACGTATTTTACAACGTTGTAATGAAGGTCGAGAAAAAGCCAAAGAAG
ATGGTAAGCATTTAGGTCGTTTTCCTGACCATGATCTGCACGAACAGATACATAATCTTGCAGGGCAGGGTCGTAATAAA
CACGCAATCAGTAAAGAGCTGGGTTGCAGTCGCACTACCGTTTATAAGGTATTGGGATAA

Upstream 100 bases:

>100_bases
TCCTAATAGGTATAATTGTGTTCATATAATCCTTGATTTATTTAATGTTCATGTCCATTATTAATTGATATGTTTATGTA
CACTTATAAAGGTAATGGTT

Downstream 100 bases:

>100_bases
AATAGGGAATTAGCGGCATAGTTGAATTGATCGCAACGATAGATTAGCAGGAGTAATTATTAAAGTATCTAGCTCGGATT
TAGCTGTTCAGTCTGCGAGG

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: Transposon Tn1000 resolvase [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MKIGYARVSTQEQTLEVQIEQLNNIKCEAIYKEHASGKSSDRKQLNLLIDFARKGDVVHVMKVDRLARNTIDALQLADKL
ANKGVGLVCHDLADLDINSDIGRVIYTTISAFAEMERKRILQRCNEGREKAKEDGKHLGRFPDHDLHEQIHNLAGQGRNK
HAISKELGCSRTTVYKVLG

Sequences:

>Translated_179_residues
MKIGYARVSTQEQTLEVQIEQLNNIKCEAIYKEHASGKSSDRKQLNLLIDFARKGDVVHVMKVDRLARNTIDALQLADKL
ANKGVGLVCHDLADLDINSDIGRVIYTTISAFAEMERKRILQRCNEGREKAKEDGKHLGRFPDHDLHEQIHNLAGQGRNK
HAISKELGCSRTTVYKVLG
>Mature_179_residues
MKIGYARVSTQEQTLEVQIEQLNNIKCEAIYKEHASGKSSDRKQLNLLIDFARKGDVVHVMKVDRLARNTIDALQLADKL
ANKGVGLVCHDLADLDINSDIGRVIYTTISAFAEMERKRILQRCNEGREKAKEDGKHLGRFPDHDLHEQIHNLAGQGRNK
HAISKELGCSRTTVYKVLG

Specific function: This protein catalyzes the site-specific recombination of the transposon and also regulates its frequency of transposition [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=181, Percent_Identity=35.9116022099448, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1787827, Length=182, Percent_Identity=31.8681318681319, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1787638, Length=182, Percent_Identity=31.8681318681319, Blast_Score=90, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 20159; Mature: 20159

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS00397 RECOMBINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGYARVSTQEQTLEVQIEQLNNIKCEAIYKEHASGKSSDRKQLNLLIDFARKGDVVHV
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEH
MKVDRLARNTIDALQLADKLANKGVGLVCHDLADLDINSDIGRVIYTTISAFAEMERKRI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LQRCNEGREKAKEDGKHLGRFPDHDLHEQIHNLAGQGRNKHAISKELGCSRTTVYKVLG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure
MKIGYARVSTQEQTLEVQIEQLNNIKCEAIYKEHASGKSSDRKQLNLLIDFARKGDVVHV
CCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEH
MKVDRLARNTIDALQLADKLANKGVGLVCHDLADLDINSDIGRVIYTTISAFAEMERKRI
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
LQRCNEGREKAKEDGKHLGRFPDHDLHEQIHNLAGQGRNKHAISKELGCSRTTVYKVLG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6292730; 8080658; 7612932; 6099239; 6088082; 2175678; 7628011; 7987224 [H]