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The map label for this gene is glpE [C]

Identifier: 119943982

GI number: 119943982

Start: 247404

End: 247814

Strand: Direct

Name: glpE [C]

Synonym: Ping_0195

Alternate gene names: 119943982

Gene position: 247404-247814 (Clockwise)

Preceding gene: 119943981

Following gene: 119943983

Centisome position: 5.43

GC content: 46.72

Gene sequence:

>411_bases
ATGCTAATAGACGGAAGAGAATTGGTCGAGTCATTACGCAGCCAAGTGCAAGAGATAAGTGCAGCACAATTGCATGACAT
ACTGCATAAAATACCGCATGATGGCACGCAGGCCGTGCTGATTGATATTCGCGAAGTAGCGGAAATAGCTGCAGGCACTA
TCGCCAACGCTGTACTTATTCCCCGTGGCGTATTAGAGATGCAAATCAGCTCAGAAGAGAGTCTTAAGCGACGTTTTCCA
ACGCTTGAAATGCTGGCGGAGCAACCTGTTTATTTACTCTGCCGCTCCGGTGCGCGCTCTGTATTATCTGCAATCTCTCT
GCAACAGATGGGATTTAAACATGTTTATTCCGTTGCTGGTGGATTTTTAGCCTGGCAGGCAGCGGGTTACGAGTGCACTA
ATAAAATATAA

Upstream 100 bases:

>100_bases
AGTACCCTGATTTTGTAACGCAGGAGCAGGGTGAAATATTGCAGGATCTTTTTGCCCCATTCTGCTCAATCGATTTGCAG
CCTATAGAGAAGGAAAAGAA

Downstream 100 bases:

>100_bases
AAATCATCCTCAAAACTTTTGATTTATTAACTTTTTTATTTATTAACTTTTTTATTTATTAACCTGGGGAGCATTTGTGA
TGGAGCCGTATTTTATCCCG

Product: rhodanese domain-containing protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: Rhodanese Domain-Containing Protein; Rhodanese-Like Domain-Containing Protein; Molybdopterin Biosynthesis Protein; Metallo-Beta-Lactamase Superfamily Protein; Rhodanese-Like Protein; Rhodanese-Related Sulfurtransferase; Rhodanese-Like Family Protein; Rhodanese/Sulfurtransferase-Like Protein; Rhodanese-Domain Protein; Thiosulfate Sulfurtransferase GlpE; Rhodanese-Like Domain Protein; Rhodanese/Moeb/Thif Domain Protein; Rhodanese-Related Sulfurtransferase Protein

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MLIDGRELVESLRSQVQEISAAQLHDILHKIPHDGTQAVLIDIREVAEIAAGTIANAVLIPRGVLEMQISSEESLKRRFP
TLEMLAEQPVYLLCRSGARSVLSAISLQQMGFKHVYSVAGGFLAWQAAGYECTNKI

Sequences:

>Translated_136_residues
MLIDGRELVESLRSQVQEISAAQLHDILHKIPHDGTQAVLIDIREVAEIAAGTIANAVLIPRGVLEMQISSEESLKRRFP
TLEMLAEQPVYLLCRSGARSVLSAISLQQMGFKHVYSVAGGFLAWQAAGYECTNKI
>Mature_136_residues
MLIDGRELVESLRSQVQEISAAQLHDILHKIPHDGTQAVLIDIREVAEIAAGTIANAVLIPRGVLEMQISSEESLKRRFP
TLEMLAEQPVYLLCRSGARSVLSAISLQQMGFKHVYSVAGGFLAWQAAGYECTNKI

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 14930; Mature: 14930

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIDGRELVESLRSQVQEISAAQLHDILHKIPHDGTQAVLIDIREVAEIAAGTIANAVLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
PRGVLEMQISSEESLKRRFPTLEMLAEQPVYLLCRSGARSVLSAISLQQMGFKHVYSVAG
CCCHHHHHCCCHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFLAWQAAGYECTNKI
HHHHHHHCCCHHCCCC
>Mature Secondary Structure
MLIDGRELVESLRSQVQEISAAQLHDILHKIPHDGTQAVLIDIREVAEIAAGTIANAVLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
PRGVLEMQISSEESLKRRFPTLEMLAEQPVYLLCRSGARSVLSAISLQQMGFKHVYSVAG
CCCHHHHHCCCHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFLAWQAAGYECTNKI
HHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA