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The map label for this gene is dnaB [H]

Identifier: 119943979

GI number: 119943979

Start: 242921

End: 244321

Strand: Reverse

Name: dnaB [H]

Synonym: Ping_0192

Alternate gene names: 119943979

Gene position: 244321-242921 (Counterclockwise)

Preceding gene: 119943980

Following gene: 119943978

Centisome position: 5.36

GC content: 44.4

Gene sequence:

>1401_bases
ATGGCAGGTAAACCTCCCTTTTCGCAGCAGAAACCCAAAATCAATACGCCAATTAAAACTGTCCCCCATTCATTTGAGGC
AGAGCAGGCAATAATTGGTGGTTTATTACTTAATAATGAAGCTTGGAATGATGTTGCCGAGCGGGTTGTTGAACGTGATT
TTTACCATAGAGCACACCGTCAAATTTTTGCAATGATTGAACGGCTGAGCAGTGAAGATATACCGGTTGATTTGGTCACT
CTCTCCGAACATCTTGAAAATAAGAAAGAGCTGGAAGGAGTCGGCGGTATTGCCTACCTTGCAGAGCTGCTGCAAAACAC
CCCCAGTGCAGCTAATATTAACAACTATTCCGATATTGTCCGAGAACGCGCCATTGTTCGCGAATTAATTGGTGTTGCCA
ATGAAATCGCCGAAGCGGGTTATGAGACAGAAGGTCGAGATAGTAATGAATTACTTGATTTTGCTGAAACGAGAGTCTTC
CAAATAGCTGAATCACGCAGCAATAAAAGCGAAGGCCCACAGGCAATTAAAGATATTTTAAAAGCGACTGTGGCAAAAAT
TGATGATTTATGCAAAAACCCGCGCGATGGTATAACCGGTTTATCTTCCGGTTATAATGATTTAGATCAGATGACGACGG
GATTCCAAGCTGGGGATTTAATTATTGTGGCAGCCCGTCCTTCCATGGGCAAAACAACCTTTGCCATGAACCTTGCCGAA
CACGCAGCGTTAAATGCCGACAAACCAACGGTTATTTTTTCACTGGAGATGCCTGCAGAACAGATAATGAACCGTATCCT
CTCCTCCCTTGGGCGAATTGATCAGGGCAAAATACGAACCGGTCAGCTGGATGAAAATGATTGGGCATCACTATCATCGA
CCATGTCTATTCTGCTGCAAAAAGGGAATATGTTTATTGATGATAGTTCTGGCCTCACGCCGACGGAATTACGCTCGCGA
GCGCGTCGTATTGCTCGAGATCATGGTGGTGTTAGTTTAATCATGGTCGATTACCTTCAGCTGATGCGCGTACCTTCATT
AAGTGAAAACCGGACCTTAGAAATATCTGAAATTTCACGTTCATTAAAAGCCCTAGCCAAAGAGCTGCGCTGTCCGGTTA
TTGCCCTTTCACAGCTAAACCGTGGCCTTGAACAACGTGCAGACAAACGCCCCATTAACTCCGACCTGCGTGAGTCTGGT
GCGATTGAGCAGGATGCCGATTTAATTATGTTTATCTATCGGGATGAAATTTATAACGAAGCAAGTGAGCATAAAGGTAT
TGCAGAGATTATTATCGGTAAACAACGTAATGGCCCGATCGGAAAGGTTCGTTTGACCTTCCAGGGCCGTTATTCACGAT
TTGATGAATATACCGGTACAGCTTACCATGGGGAAGATTGA

Upstream 100 bases:

>100_bases
TGCGAGTGCAAGCATCACTCAAAAAGTTCCCTATCTTATATTTATTAACGGGAACCCGTTCCCGTTAATAGTAAATATTC
TTTTCAGTTAAGGATTTAAA

Downstream 100 bases:

>100_bases
TCATTTCTCAGGTGCCCGTTAAGCAATTACTTAAACCAATTTATAATAAATAGAGTTTAATTATGGATACCATACGCGCA
GTTATTAACTTAAAGGCATT

Product: replicative DNA helicase DnaB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MAGKPPFSQQKPKINTPIKTVPHSFEAEQAIIGGLLLNNEAWNDVAERVVERDFYHRAHRQIFAMIERLSSEDIPVDLVT
LSEHLENKKELEGVGGIAYLAELLQNTPSAANINNYSDIVRERAIVRELIGVANEIAEAGYETEGRDSNELLDFAETRVF
QIAESRSNKSEGPQAIKDILKATVAKIDDLCKNPRDGITGLSSGYNDLDQMTTGFQAGDLIIVAARPSMGKTTFAMNLAE
HAALNADKPTVIFSLEMPAEQIMNRILSSLGRIDQGKIRTGQLDENDWASLSSTMSILLQKGNMFIDDSSGLTPTELRSR
ARRIARDHGGVSLIMVDYLQLMRVPSLSENRTLEISEISRSLKALAKELRCPVIALSQLNRGLEQRADKRPINSDLRESG
AIEQDADLIMFIYRDEIYNEASEHKGIAEIIIGKQRNGPIGKVRLTFQGRYSRFDEYTGTAYHGED

Sequences:

>Translated_466_residues
MAGKPPFSQQKPKINTPIKTVPHSFEAEQAIIGGLLLNNEAWNDVAERVVERDFYHRAHRQIFAMIERLSSEDIPVDLVT
LSEHLENKKELEGVGGIAYLAELLQNTPSAANINNYSDIVRERAIVRELIGVANEIAEAGYETEGRDSNELLDFAETRVF
QIAESRSNKSEGPQAIKDILKATVAKIDDLCKNPRDGITGLSSGYNDLDQMTTGFQAGDLIIVAARPSMGKTTFAMNLAE
HAALNADKPTVIFSLEMPAEQIMNRILSSLGRIDQGKIRTGQLDENDWASLSSTMSILLQKGNMFIDDSSGLTPTELRSR
ARRIARDHGGVSLIMVDYLQLMRVPSLSENRTLEISEISRSLKALAKELRCPVIALSQLNRGLEQRADKRPINSDLRESG
AIEQDADLIMFIYRDEIYNEASEHKGIAEIIIGKQRNGPIGKVRLTFQGRYSRFDEYTGTAYHGED
>Mature_465_residues
AGKPPFSQQKPKINTPIKTVPHSFEAEQAIIGGLLLNNEAWNDVAERVVERDFYHRAHRQIFAMIERLSSEDIPVDLVTL
SEHLENKKELEGVGGIAYLAELLQNTPSAANINNYSDIVRERAIVRELIGVANEIAEAGYETEGRDSNELLDFAETRVFQ
IAESRSNKSEGPQAIKDILKATVAKIDDLCKNPRDGITGLSSGYNDLDQMTTGFQAGDLIIVAARPSMGKTTFAMNLAEH
AALNADKPTVIFSLEMPAEQIMNRILSSLGRIDQGKIRTGQLDENDWASLSSTMSILLQKGNMFIDDSSGLTPTELRSRA
RRIARDHGGVSLIMVDYLQLMRVPSLSENRTLEISEISRSLKALAKELRCPVIALSQLNRGLEQRADKRPINSDLRESGA
IEQDADLIMFIYRDEIYNEASEHKGIAEIIIGKQRNGPIGKVRLTFQGRYSRFDEYTGTAYHGED

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=471, Percent_Identity=68.5774946921444, Blast_Score=669, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51831; Mature: 51700

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKPPFSQQKPKINTPIKTVPHSFEAEQAIIGGLLLNNEAWNDVAERVVERDFYHRAHR
CCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
QIFAMIERLSSEDIPVDLVTLSEHLENKKELEGVGGIAYLAELLQNTPSAANINNYSDIV
HHHHHHHHHCCCCCCEEEEHHHHHHCCHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHH
RERAIVRELIGVANEIAEAGYETEGRDSNELLDFAETRVFQIAESRSNKSEGPQAIKDIL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
KATVAKIDDLCKNPRDGITGLSSGYNDLDQMTTGFQAGDLIIVAARPSMGKTTFAMNLAE
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHH
HAALNADKPTVIFSLEMPAEQIMNRILSSLGRIDQGKIRTGQLDENDWASLSSTMSILLQ
HHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KGNMFIDDSSGLTPTELRSRARRIARDHGGVSLIMVDYLQLMRVPSLSENRTLEISEISR
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCEEHHHHHH
SLKALAKELRCPVIALSQLNRGLEQRADKRPINSDLRESGAIEQDADLIMFIYRDEIYNE
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEHHHHHHH
ASEHKGIAEIIIGKQRNGPIGKVRLTFQGRYSRFDEYTGTAYHGED
HHHCCCHHHHHCCCCCCCCCEEEEEEECCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AGKPPFSQQKPKINTPIKTVPHSFEAEQAIIGGLLLNNEAWNDVAERVVERDFYHRAHR
CCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
QIFAMIERLSSEDIPVDLVTLSEHLENKKELEGVGGIAYLAELLQNTPSAANINNYSDIV
HHHHHHHHHCCCCCCEEEEHHHHHHCCHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHH
RERAIVRELIGVANEIAEAGYETEGRDSNELLDFAETRVFQIAESRSNKSEGPQAIKDIL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
KATVAKIDDLCKNPRDGITGLSSGYNDLDQMTTGFQAGDLIIVAARPSMGKTTFAMNLAE
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHH
HAALNADKPTVIFSLEMPAEQIMNRILSSLGRIDQGKIRTGQLDENDWASLSSTMSILLQ
HHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KGNMFIDDSSGLTPTELRSRARRIARDHGGVSLIMVDYLQLMRVPSLSENRTLEISEISR
CCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCEEHHHHHH
SLKALAKELRCPVIALSQLNRGLEQRADKRPINSDLRESGAIEQDADLIMFIYRDEIYNE
HHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEHHHHHHH
ASEHKGIAEIIIGKQRNGPIGKVRLTFQGRYSRFDEYTGTAYHGED
HHHCCCHHHHHCCCCCCCCCEEEEEEECCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]