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The map label for this gene is gloA [H]

Identifier: 119943956

GI number: 119943956

Start: 219431

End: 219844

Strand: Reverse

Name: gloA [H]

Synonym: Ping_0169

Alternate gene names: 119943956

Gene position: 219844-219431 (Counterclockwise)

Preceding gene: 119943957

Following gene: 119943944

Centisome position: 4.82

GC content: 39.86

Gene sequence:

>414_bases
ATGCGCTTATTACATACCATGTTACGTGTTGCAGATTTACAAAAATCGATCGATTTTTACAGCAATATTTTACAGATGAA
ATTACTGCGTCAATCTGAAAATGCTGATTATAAATATACGCTGGCATTTTTAGGTTATGGGGACGAGTCTGATACTACTG
TACTCGAGCTCACTTACAATTGGGGCACAACAGAATATGATTTAGGCAATGCTTACGGACATATCGCTATTGAAACCGAT
GATATTTACGCTACCTGTGAAATGATAAAAAAGATGGGCGGTCAAGTTACCCGTGAAGCAGGGCCGGTTAAAGGCGGCAC
AACAGTGATCGCTTTTGTTAAGGATCCCGATGGTTATCAGATCGAATTAATTAATAAAAAAGACGCTGGCAAGGGTTTGG
GTGCAGGGTCATAA

Upstream 100 bases:

>100_bases
ATTATCTAATTTAGCCACCTGTAAGGTGGCTTTTTTATAGCCAATATTTATAGCACCTGGTTATTGTTATGCACCTCCAC
ATTAAAAATAGGAATTCAAA

Downstream 100 bases:

>100_bases
AGCACTTTGCATAAAAAAACGCAGTGGTTGTTATTAACAAACACTGCGCTTTTTATTTTTATTCAATAAACAGGCTATTT
TGGTAAAAAACCGACTTTAT

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MRLLHTMLRVADLQKSIDFYSNILQMKLLRQSENADYKYTLAFLGYGDESDTTVLELTYNWGTTEYDLGNAYGHIAIETD
DIYATCEMIKKMGGQVTREAGPVKGGTTVIAFVKDPDGYQIELINKKDAGKGLGAGS

Sequences:

>Translated_137_residues
MRLLHTMLRVADLQKSIDFYSNILQMKLLRQSENADYKYTLAFLGYGDESDTTVLELTYNWGTTEYDLGNAYGHIAIETD
DIYATCEMIKKMGGQVTREAGPVKGGTTVIAFVKDPDGYQIELINKKDAGKGLGAGS
>Mature_137_residues
MRLLHTMLRVADLQKSIDFYSNILQMKLLRQSENADYKYTLAFLGYGDESDTTVLELTYNWGTTEYDLGNAYGHIAIETD
DIYATCEMIKKMGGQVTREAGPVKGGTTVIAFVKDPDGYQIELINKKDAGKGLGAGS

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=147, Percent_Identity=36.0544217687075, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1787940, Length=134, Percent_Identity=70.1492537313433, Blast_Score=206, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI17552228, Length=133, Percent_Identity=35.3383458646617, Blast_Score=82, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323639, Length=139, Percent_Identity=43.8848920863309, Blast_Score=106, Evalue=1e-24,
Organism=Drosophila melanogaster, GI20129743, Length=145, Percent_Identity=35.1724137931034, Blast_Score=89, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19920404, Length=139, Percent_Identity=30.9352517985612, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 15171; Mature: 15171

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLHTMLRVADLQKSIDFYSNILQMKLLRQSENADYKYTLAFLGYGDESDTTVLELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEEEC
WGTTEYDLGNAYGHIAIETDDIYATCEMIKKMGGQVTREAGPVKGGTTVIAFVKDPDGYQ
CCCEEEECCCCEEEEEEECCCHHHHHHHHHHHCCEEECCCCCCCCCCEEEEEEECCCCCE
IELINKKDAGKGLGAGS
EEEECCCCCCCCCCCCC
>Mature Secondary Structure
MRLLHTMLRVADLQKSIDFYSNILQMKLLRQSENADYKYTLAFLGYGDESDTTVLELTYN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEEEEC
WGTTEYDLGNAYGHIAIETDDIYATCEMIKKMGGQVTREAGPVKGGTTVIAFVKDPDGYQ
CCCEEEECCCCEEEEEEECCCHHHHHHHHHHHCCEEECCCCCCCCCCEEEEEEECCCCCE
IELINKKDAGKGLGAGS
EEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]