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The map label for this gene is dut

Identifier: 119943851

GI number: 119943851

Start: 79804

End: 80259

Strand: Direct

Name: dut

Synonym: Ping_0058

Alternate gene names: 119943851

Gene position: 79804-80259 (Clockwise)

Preceding gene: 119943850

Following gene: 119943852

Centisome position: 1.75

GC content: 44.74

Gene sequence:

>456_bases
ATGAAACAGATAGAGCTTAAAATTCTCGATCCTCGCATTGGTAAGGAATTCCCGCTTCCTGAGCATGCGACGGCGGGATC
TGCTGGGATGGATTTGCGTGCTTGTATTGATCAGGCCTTAATTATTAAGCCTGGAGAAACACAACTAATTCCAACGGGTA
TTGCAATCCATATTAGCGATCCTGGACTTGCGGCTACCATTTTACCGCGCTCTGGATTGGGTCATAAGCATGGTATCGTT
TTGGGAAATTTAGTGGGTTTAATTGATTCTGATTATCAAGGTCCGCTAATGGTTTCTTGTTGGAATCGCAGCACAGAAAG
TTTCCGCCTTGAGCCGGGAGAGCGATTAGCGCAGTTGGTTTTTTTACCTGTTGTACAGGCAACATTCGAAATTGTTGATG
AGTTTAAGAGCTCAGAACGAGGAGAGGGTGGATTTGGTCACTCAGGGAAACATTAA

Upstream 100 bases:

>100_bases
AACGATTTGATATAACGCTTGCCGATAAAAAGGCGGTTGCCAGCCAGCTACTTAAAATAATAAGCCAACAATACGCTTTG
AAAAATAAAGGATTTAAATA

Downstream 100 bases:

>100_bases
TTTTAGTTTAAAACGACAAAGGAAGTTGTAGATTATGCTGGCAAAAACAACCAAAAAGAAAGTAAATCGCAAGGAAGATA
TTTTACACGCATTGGCTAAA

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase Dut

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MKQIELKILDPRIGKEFPLPEHATAGSAGMDLRACIDQALIIKPGETQLIPTGIAIHISDPGLAATILPRSGLGHKHGIV
LGNLVGLIDSDYQGPLMVSCWNRSTESFRLEPGERLAQLVFLPVVQATFEIVDEFKSSERGEGGFGHSGKH

Sequences:

>Translated_151_residues
MKQIELKILDPRIGKEFPLPEHATAGSAGMDLRACIDQALIIKPGETQLIPTGIAIHISDPGLAATILPRSGLGHKHGIV
LGNLVGLIDSDYQGPLMVSCWNRSTESFRLEPGERLAQLVFLPVVQATFEIVDEFKSSERGEGGFGHSGKH
>Mature_151_residues
MKQIELKILDPRIGKEFPLPEHATAGSAGMDLRACIDQALIIKPGETQLIPTGIAIHISDPGLAATILPRSGLGHKHGIV
LGNLVGLIDSDYQGPLMVSCWNRSTESFRLEPGERLAQLVFLPVVQATFEIVDEFKSSERGEGGFGHSGKH

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=140, Percent_Identity=35.7142857142857, Blast_Score=81, Evalue=3e-16,
Organism=Homo sapiens, GI4503423, Length=140, Percent_Identity=35.7142857142857, Blast_Score=81, Evalue=3e-16,
Organism=Homo sapiens, GI70906441, Length=140, Percent_Identity=35.7142857142857, Blast_Score=80, Evalue=9e-16,
Organism=Escherichia coli, GI1790071, Length=151, Percent_Identity=68.8741721854305, Blast_Score=237, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI71988561, Length=132, Percent_Identity=36.3636363636364, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6319729, Length=134, Percent_Identity=36.5671641791045, Blast_Score=76, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19921126, Length=130, Percent_Identity=32.3076923076923, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583610, Length=130, Percent_Identity=32.3076923076923, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_PSYIN (A1SR17)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_941531.1
- ProteinModelPortal:   A1SR17
- SMR:   A1SR17
- STRING:   A1SR17
- GeneID:   4624455
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0058
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- PhylomeDB:   A1SR17
- BioCyc:   PING357804:PING_0058-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16275; Mature: 16275

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: BINDING 83-83 BINDING 97-97

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQIELKILDPRIGKEFPLPEHATAGSAGMDLRACIDQALIIKPGETQLIPTGIAIHISD
CCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCEEECCCEEEEECC
PGLAATILPRSGLGHKHGIVLGNLVGLIDSDYQGPLMVSCWNRSTESFRLEPGERLAQLV
CCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCEEEECCHHHHHHHH
FLPVVQATFEIVDEFKSSERGEGGFGHSGKH
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKQIELKILDPRIGKEFPLPEHATAGSAGMDLRACIDQALIIKPGETQLIPTGIAIHISD
CCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCEEECCCEEEEECC
PGLAATILPRSGLGHKHGIVLGNLVGLIDSDYQGPLMVSCWNRSTESFRLEPGERLAQLV
CCEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCEEEECCHHHHHHHH
FLPVVQATFEIVDEFKSSERGEGGFGHSGKH
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA