Click here to switch to the map view.

The map label for this gene is ddl [H]

Identifier: 119943830

GI number: 119943830

Start: 53800

End: 54942

Strand: Direct

Name: ddl [H]

Synonym: Ping_0036

Alternate gene names: 119943830

Gene position: 53800-54942 (Clockwise)

Preceding gene: 119943829

Following gene: 119943835

Centisome position: 1.18

GC content: 34.73

Gene sequence:

>1143_bases
ATGTCAGATAGCAGAATGAAAGTAGCAGTTGTTTTTGGTGGTAAATCTACAGAGCATGATGTGTCAATTATTTCTGCAGG
ACATATACTTGCAAATATTGATATGGAAAAATATTTTGTTGAACCATTTTATGTTAAGAAAGATGGGACATTTGCAGATC
ACCAAGAATTAACACTTTGCCTAGAAAATCTATTAAATGAGCATAGAGATAAAATCTTTCCAGCAATGAAAGAAGGTGAA
ATTGAGCTGAAAAAGTGGATAGCCAAAGGTACTTCTTCAGATTTTTTAGGCGGAATTGCTGGGAAAAAATTTGATATTGT
GTTTCCTGTGCTTCACGGACTAAACGGAGAAGATGGAACCATTCAAGGAATGCTTGAATTCATGGGAGTACCTTATGTTG
GCTGTGGTGTAAGTGCATCTGCTTTTTGTATAGATAAAGAAGCCACAAAGGTAATATGCCAGGTAAATGATATACCTGTG
ATGGATTATACTGCAGTAAAAAAATATGAGTGGGAAAATTCAACAGAAAATATAATCAATGATATTCAGAAAAAAATGAA
TTACCCGTTTTATGTAAAGCCTGCGAGACTCGGTTCTTCAATTGGTATTTCAAGAGCGGAAAATACTGTTGAACTTAAAA
CTTCACTTAAAGACGCATTTAAATATGACTGCAAAGTGGTAGTCGAGCAAGGAGTTGAGTCCCCTAGGGAGCTTACTGTT
GGTGTGATGGGGATTGATGACCAAGTATCATTAAGTGCAATAGGTGAATTTAGCCGTGGGAGTAATTTGTATTTTAATTT
TGATTCAAAATATGGCAAGACCGGCCATAAAGGGCTTGTGCCTGCTCCGCTGGAAGAAAATATACAAGAAATTATAGAAA
AATATTCTCATCTGATTTTTAAAAAATTTGAGTTATCAGGTTTTGCAAGAATAGATTATTTTTTATGTGGTGACAAAGTG
TACCTGAATGAGATCAATACGATGCCAGGATTTGAAACGGGTGATACTTTTATGCGAATATGGCAAAATAAAGGCGTGGA
TATGCAAAAATTTATTGATAAGGCAATAGAGTACGGGGTTTTAAGCTTTCATAGCAAATCAACAAGGTTATATGAAATAG
AGATTAAGAGCTTAATAGAATAA

Upstream 100 bases:

>100_bases
GTTTTTCAGATAGTATCTTTGTTATTGGTTTGTTCACAATGGCATCATCTGTCGCTTATTTATTTATAAGTAAAAAAAAT
CCACAATAGGTTTAATTATT

Downstream 100 bases:

>100_bases
TACCGGGTTGGCTCTGCCTTGTATAAAGCACCCATAAATTGCTGCAAAAATGCACATAGTAGTTCGGCAACCATTTATGC
ATACATGAGACAGTAAATTG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSDSRMKVAVVFGGKSTEHDVSIISAGHILANIDMEKYFVEPFYVKKDGTFADHQELTLCLENLLNEHRDKIFPAMKEGE
IELKKWIAKGTSSDFLGGIAGKKFDIVFPVLHGLNGEDGTIQGMLEFMGVPYVGCGVSASAFCIDKEATKVICQVNDIPV
MDYTAVKKYEWENSTENIINDIQKKMNYPFYVKPARLGSSIGISRAENTVELKTSLKDAFKYDCKVVVEQGVESPRELTV
GVMGIDDQVSLSAIGEFSRGSNLYFNFDSKYGKTGHKGLVPAPLEENIQEIIEKYSHLIFKKFELSGFARIDYFLCGDKV
YLNEINTMPGFETGDTFMRIWQNKGVDMQKFIDKAIEYGVLSFHSKSTRLYEIEIKSLIE

Sequences:

>Translated_380_residues
MSDSRMKVAVVFGGKSTEHDVSIISAGHILANIDMEKYFVEPFYVKKDGTFADHQELTLCLENLLNEHRDKIFPAMKEGE
IELKKWIAKGTSSDFLGGIAGKKFDIVFPVLHGLNGEDGTIQGMLEFMGVPYVGCGVSASAFCIDKEATKVICQVNDIPV
MDYTAVKKYEWENSTENIINDIQKKMNYPFYVKPARLGSSIGISRAENTVELKTSLKDAFKYDCKVVVEQGVESPRELTV
GVMGIDDQVSLSAIGEFSRGSNLYFNFDSKYGKTGHKGLVPAPLEENIQEIIEKYSHLIFKKFELSGFARIDYFLCGDKV
YLNEINTMPGFETGDTFMRIWQNKGVDMQKFIDKAIEYGVLSFHSKSTRLYEIEIKSLIE
>Mature_379_residues
SDSRMKVAVVFGGKSTEHDVSIISAGHILANIDMEKYFVEPFYVKKDGTFADHQELTLCLENLLNEHRDKIFPAMKEGEI
ELKKWIAKGTSSDFLGGIAGKKFDIVFPVLHGLNGEDGTIQGMLEFMGVPYVGCGVSASAFCIDKEATKVICQVNDIPVM
DYTAVKKYEWENSTENIINDIQKKMNYPFYVKPARLGSSIGISRAENTVELKTSLKDAFKYDCKVVVEQGVESPRELTVG
VMGIDDQVSLSAIGEFSRGSNLYFNFDSKYGKTGHKGLVPAPLEENIQEIIEKYSHLIFKKFELSGFARIDYFLCGDKVY
LNEINTMPGFETGDTFMRIWQNKGVDMQKFIDKAIEYGVLSFHSKSTRLYEIEIKSLIE

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=370, Percent_Identity=31.8918918918919, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1786280, Length=356, Percent_Identity=27.5280898876405, Blast_Score=134, Evalue=9e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 42743; Mature: 42612

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSRMKVAVVFGGKSTEHDVSIISAGHILANIDMEKYFVEPFYVKKDGTFADHQELTLC
CCCCCEEEEEEECCCCCCCCEEEEECCCEEEECCHHHEECCEEEEECCCCCCCHHHHHHH
LENLLNEHRDKIFPAMKEGEIELKKWIAKGTSSDFLGGIAGKKFDIVFPVLHGLNGEDGT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCEEEEEHHHHCCCCCCCH
IQGMLEFMGVPYVGCGVSASAFCIDKEATKVICQVNDIPVMDYTAVKKYEWENSTENIIN
HHHHHHHHCCCEECCCCCCCEEEECCCCCEEEEEECCCCCCCHHHHEEECCCCCHHHHHH
DIQKKMNYPFYVKPARLGSSIGISRAENTVELKTSLKDAFKYDCKVVVEQGVESPRELTV
HHHHHCCCCEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
GVMGIDDQVSLSAIGEFSRGSNLYFNFDSKYGKTGHKGLVPAPLEENIQEIIEKYSHLIF
EEEECCCCCCHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KKFELSGFARIDYFLCGDKVYLNEINTMPGFETGDTFMRIWQNKGVDMQKFIDKAIEYGV
HHHCCCCEEEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCH
LSFHSKSTRLYEIEIKSLIE
HEEECCCCEEEEEEEHHHCC
>Mature Secondary Structure 
SDSRMKVAVVFGGKSTEHDVSIISAGHILANIDMEKYFVEPFYVKKDGTFADHQELTLC
CCCCEEEEEEECCCCCCCCEEEEECCCEEEECCHHHEECCEEEEECCCCCCCHHHHHHH
LENLLNEHRDKIFPAMKEGEIELKKWIAKGTSSDFLGGIAGKKFDIVFPVLHGLNGEDGT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCEEEEEHHHHCCCCCCCH
IQGMLEFMGVPYVGCGVSASAFCIDKEATKVICQVNDIPVMDYTAVKKYEWENSTENIIN
HHHHHHHHCCCEECCCCCCCEEEECCCCCEEEEEECCCCCCCHHHHEEECCCCCHHHHHH
DIQKKMNYPFYVKPARLGSSIGISRAENTVELKTSLKDAFKYDCKVVVEQGVESPRELTV
HHHHHCCCCEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
GVMGIDDQVSLSAIGEFSRGSNLYFNFDSKYGKTGHKGLVPAPLEENIQEIIEKYSHLIF
EEEECCCCCCHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KKFELSGFARIDYFLCGDKVYLNEINTMPGFETGDTFMRIWQNKGVDMQKFIDKAIEYGV
HHHCCCCEEEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCH
LSFHSKSTRLYEIEIKSLIE
HEEECCCCEEEEEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA