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The map label for this gene is xthA2 [H]

Identifier: 119899953

GI number: 119899953

Start: 4023919

End: 4024701

Strand: Direct

Name: xthA2 [H]

Synonym: azo3664

Alternate gene names: 119899953

Gene position: 4023919-4024701 (Clockwise)

Preceding gene: 119899945

Following gene: 119899954

Centisome position: 91.95

GC content: 66.16

Gene sequence:

>783_bases
ATGTTACGCATCATCTCCGCCAACCTCAACGGCATCCGCTCCGCCACCACCAAAGGCTTTCTCGACTGGCTGCCCGCCCA
AAACGCCGACGTCGTATGCGTTCAGGAACTCAAGGCGCAGGCAGGCGACCTCACCGACGCGATGCGCGCCCCGGCGGGCT
ACCAGGGCTGGTTCCACTACGCCGAGAAGAAGGGCTACAGCGGCGTCGGCATCTACAGCCGCAAGGCGCCGGACCGGGTG
GTGGAAGGGCTGGGCATCGCCGACATCGACGCCGAGGGTCGCTACCTGCAGGCCGATTTCGGGGCCCTCTCGGTGATCTC
GCTCTACCTGCCCTCGGGCTCCAGTTCGGAAGAACGCCAGCAGGCCAAGTTCAGCTTCATGGAGCGCTTCATGCCCCGCC
TCGCCGAACTGCGGCGCTGCGGCCAGGAGGTCGTGATCTGCGGCGACTGGAACATCGCCCACCGCGAAATCGACCTGAAG
AACTGGAAGAGCAACCAGAAGAACTCGGGCTTCCTGCCCGAGGAACGTGCCTGGATCGGCACGCTGTTCGACACCCACGG
CTGGGTGGATGTCTACCGCGCGCTGTACCCCGAGGCCACGGGCGACAGCTACACCTGGTGGTCCAACCGCGGCCAGGCAT
GGGCCAAGAACGTCGGGTGGCGGATCGACTACCAGATCGCCACCCCGGGCATCGCCGCGCGCGCACGCGCCGGTTCGGTG
TACAAGGCGCAGCGCTTTTCCGACCACGCTCCGCTCGTCGTGGACTACGACGGCGATCTGTGA

Upstream 100 bases:

>100_bases
CCCGGCTAGAATCCGGGCCCTTGTGAACGGCTTCCACGACTTTATCCAATTCGCATCGAACGGCACCCAGGCGCGCCGTT
CATAAGGAAAAAACGGGACC

Downstream 100 bases:

>100_bases
GGACCTGCCCGCCTTTGCGTTGCCTTGCCATGGTGCTATACAACAGTGAGTCTCATCTTCTCGATATGAATAGACACTGA
TATTCCGCGGCGATAATCCC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLRIISANLNGIRSATTKGFLDWLPAQNADVVCVQELKAQAGDLTDAMRAPAGYQGWFHYAEKKGYSGVGIYSRKAPDRV
VEGLGIADIDAEGRYLQADFGALSVISLYLPSGSSSEERQQAKFSFMERFMPRLAELRRCGQEVVICGDWNIAHREIDLK
NWKSNQKNSGFLPEERAWIGTLFDTHGWVDVYRALYPEATGDSYTWWSNRGQAWAKNVGWRIDYQIATPGIAARARAGSV
YKAQRFSDHAPLVVDYDGDL

Sequences:

>Translated_260_residues
MLRIISANLNGIRSATTKGFLDWLPAQNADVVCVQELKAQAGDLTDAMRAPAGYQGWFHYAEKKGYSGVGIYSRKAPDRV
VEGLGIADIDAEGRYLQADFGALSVISLYLPSGSSSEERQQAKFSFMERFMPRLAELRRCGQEVVICGDWNIAHREIDLK
NWKSNQKNSGFLPEERAWIGTLFDTHGWVDVYRALYPEATGDSYTWWSNRGQAWAKNVGWRIDYQIATPGIAARARAGSV
YKAQRFSDHAPLVVDYDGDL
>Mature_260_residues
MLRIISANLNGIRSATTKGFLDWLPAQNADVVCVQELKAQAGDLTDAMRAPAGYQGWFHYAEKKGYSGVGIYSRKAPDRV
VEGLGIADIDAEGRYLQADFGALSVISLYLPSGSSSEERQQAKFSFMERFMPRLAELRRCGQEVVICGDWNIAHREIDLK
NWKSNQKNSGFLPEERAWIGTLFDTHGWVDVYRALYPEATGDSYTWWSNRGQAWAKNVGWRIDYQIATPGIAARARAGSV
YKAQRFSDHAPLVVDYDGDL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=255, Percent_Identity=35.2941176470588, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI18375503, Length=255, Percent_Identity=35.2941176470588, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI18375501, Length=255, Percent_Identity=35.2941176470588, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI18375507, Length=314, Percent_Identity=26.4331210191083, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=29.8507462686567, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71989536, Length=260, Percent_Identity=28.4615384615385, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI221330655, Length=256, Percent_Identity=32.03125, Blast_Score=134, Evalue=5e-32,
Organism=Drosophila melanogaster, GI17136678, Length=254, Percent_Identity=32.6771653543307, Blast_Score=134, Evalue=9e-32,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29124; Mature: 29124

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIISANLNGIRSATTKGFLDWLPAQNADVVCVQELKAQAGDLTDAMRAPAGYQGWFHY
CEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHCCCHHHHHHCCCCCHHHHHH
AEKKGYSGVGIYSRKAPDRVVEGLGIADIDAEGRYLQADFGALSVISLYLPSGSSSEERQ
HHHCCCCCCCEECCCCHHHHHHCCCCEEECCCCCEEEECCCCEEEEEEEECCCCCHHHHH
QAKFSFMERFMPRLAELRRCGQEVVICGDWNIAHREIDLKNWKSNQKNSGFLPEERAWIG
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEHHHCCCCCCCCCCCCCCHHHEE
TLFDTHGWVDVYRALYPEATGDSYTWWSNRGQAWAKNVGWRIDYQIATPGIAARARAGSV
HHHHCCCHHHHHHHHCCCCCCCCEEEECCCCCHHHHCCCEEEEEEEECCCHHHHHCCCCC
YKAQRFSDHAPLVVDYDGDL
HHHHHCCCCCCEEEEECCCC
>Mature Secondary Structure
MLRIISANLNGIRSATTKGFLDWLPAQNADVVCVQELKAQAGDLTDAMRAPAGYQGWFHY
CEEEEECCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHCCCHHHHHHCCCCCHHHHHH
AEKKGYSGVGIYSRKAPDRVVEGLGIADIDAEGRYLQADFGALSVISLYLPSGSSSEERQ
HHHCCCCCCCEECCCCHHHHHHCCCCEEECCCCCEEEECCCCEEEEEEEECCCCCHHHHH
QAKFSFMERFMPRLAELRRCGQEVVICGDWNIAHREIDLKNWKSNQKNSGFLPEERAWIG
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEHHHCCCCCCCCCCCCCCHHHEE
TLFDTHGWVDVYRALYPEATGDSYTWWSNRGQAWAKNVGWRIDYQIATPGIAARARAGSV
HHHHCCCHHHHHHHHCCCCCCCCEEEECCCCCHHHHCCCEEEEEEEECCCHHHHHCCCCC
YKAQRFSDHAPLVVDYDGDL
HHHHHCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]