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The map label for this gene is fcl1 [H]

Identifier: 119899875

GI number: 119899875

Start: 3936457

End: 3937452

Strand: Reverse

Name: fcl1 [H]

Synonym: azo3586

Alternate gene names: 119899875

Gene position: 3937452-3936457 (Counterclockwise)

Preceding gene: 119899876

Following gene: 119899874

Centisome position: 89.98

GC content: 60.54

Gene sequence:

>996_bases
ATGAACTCGTCAAGACGCATGGCTATTCGGCCTATGACTACCACGAATAATCTGCCCTTGGACAAGGATGCCCGGATCTA
TGTTGCGGGGCACCGCGGCATGGTCGGCTCCGCCATCGAGCGGCGTCTGGTGGCTGCCGGTTATTCCAATGTGCTTGCGC
GTAGCCATGACGAGCTCGACCTGACCAACCAGGCCGATGTCTTCGCGTTCCTCCGTGAGGAGCGGCCGGATTTCATTTTC
CTCGCCGCGGGCCGAGTGGGAGGTATTCACGCCAACAATACCTATCGCGCGGATTTCATCTATCGCAACCTGATGATCGA
GGCCAACGTCATCCACGGTGCCTGGCTCGCCGGGGTCAGACGCTTGCTCTTCATCGGTTCGAGCTGTATCTACCCGCGTC
AGTGCCCGCAACCCATCCACGAAGACTATCTCCTGAGCGGGCCGCTCGAGAAGACCAACGAGCCGTACGCGCTGGCGAAG
ATCGCGGGTGTGAAGCTGTGCGAGAGCTACAACGCGCAGTACGGCACTTCGTACTTCTCGGTGATGGCGACCAATCTCTA
CGGTCCTAACGACAACTACGACCCCGACAACAGCCACGTGCTACCTTCGCTGATCCGCAAGGCGCACGACGCCAAGGTCA
CGGGAAGCCATGAACTCGTGGTGTGGGGCTCCGGGGCGCCACGGCGTGAGTTTCTCTACGTAGACGACATGGCGGATGCA
TGTGTATTCCTGATGGAGCGCGGTCTGGGCGACGGCCTCGTGAATGTCGGCACTGGCGCTGATCTCACCATTCGCGAACT
CGCGGAAATGGTGATGGACGTGGTGGGGTTCGAGGGTGCGATCCGCTTCGACAGCGCAAAGCCGGACGGAACGCCGCGCA
AGTTGCTCAACGTCGACCGGATGACTGAAATGGGTTGGCAGGCGAAGACAGGCCTGCGCGAGGGAATCGCGAAGGCCTAC
CAGGACTATCTCCAGCGTTTCGGGTCTCCGCGATGA

Upstream 100 bases:

>100_bases
GCCTCCAAGGCCCGGCGCCTGCTTGGTTGGACACCACGGATTTCGTTTAGCGAACTTGTGTCGGAGATGATGCGCGAAGA
CATGAAGGCGGCGGAGCGTG

Downstream 100 bases:

>100_bases
CGGCACCGTTGGTGACCATCGCCGTTCCGTCCTACAACCAAGGGCGCTTTCTCGATGCCGCCCTGGCCTCGATTTTCGCG
CAGGACCTCCCACTGGAGGT

Product: putative GDP-fucose synthetase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MNSSRRMAIRPMTTTNNLPLDKDARIYVAGHRGMVGSAIERRLVAAGYSNVLARSHDELDLTNQADVFAFLREERPDFIF
LAAGRVGGIHANNTYRADFIYRNLMIEANVIHGAWLAGVRRLLFIGSSCIYPRQCPQPIHEDYLLSGPLEKTNEPYALAK
IAGVKLCESYNAQYGTSYFSVMATNLYGPNDNYDPDNSHVLPSLIRKAHDAKVTGSHELVVWGSGAPRREFLYVDDMADA
CVFLMERGLGDGLVNVGTGADLTIRELAEMVMDVVGFEGAIRFDSAKPDGTPRKLLNVDRMTEMGWQAKTGLREGIAKAY
QDYLQRFGSPR

Sequences:

>Translated_331_residues
MNSSRRMAIRPMTTTNNLPLDKDARIYVAGHRGMVGSAIERRLVAAGYSNVLARSHDELDLTNQADVFAFLREERPDFIF
LAAGRVGGIHANNTYRADFIYRNLMIEANVIHGAWLAGVRRLLFIGSSCIYPRQCPQPIHEDYLLSGPLEKTNEPYALAK
IAGVKLCESYNAQYGTSYFSVMATNLYGPNDNYDPDNSHVLPSLIRKAHDAKVTGSHELVVWGSGAPRREFLYVDDMADA
CVFLMERGLGDGLVNVGTGADLTIRELAEMVMDVVGFEGAIRFDSAKPDGTPRKLLNVDRMTEMGWQAKTGLREGIAKAY
QDYLQRFGSPR
>Mature_331_residues
MNSSRRMAIRPMTTTNNLPLDKDARIYVAGHRGMVGSAIERRLVAAGYSNVLARSHDELDLTNQADVFAFLREERPDFIF
LAAGRVGGIHANNTYRADFIYRNLMIEANVIHGAWLAGVRRLLFIGSSCIYPRQCPQPIHEDYLLSGPLEKTNEPYALAK
IAGVKLCESYNAQYGTSYFSVMATNLYGPNDNYDPDNSHVLPSLIRKAHDAKVTGSHELVVWGSGAPRREFLYVDDMADA
CVFLMERGLGDGLVNVGTGADLTIRELAEMVMDVVGFEGAIRFDSAKPDGTPRKLLNVDRMTEMGWQAKTGLREGIAKAY
QDYLQRFGSPR

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=276, Percent_Identity=35.1449275362319, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI7657641, Length=342, Percent_Identity=23.0994152046784, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI42516563, Length=315, Percent_Identity=25.3968253968254, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1788365, Length=310, Percent_Identity=54.8387096774194, Blast_Score=352, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI17554428, Length=313, Percent_Identity=28.1150159744409, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI115532424, Length=181, Percent_Identity=28.1767955801105, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922778, Length=319, Percent_Identity=29.153605015674, Blast_Score=139, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 36789; Mature: 36789

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSRRMAIRPMTTTNNLPLDKDARIYVAGHRGMVGSAIERRLVAAGYSNVLARSHDELD
CCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHHCCCCCCC
LTNQADVFAFLREERPDFIFLAAGRVGGIHANNTYRADFIYRNLMIEANVIHGAWLAGVR
CCCCHHHHHHHHHCCCCEEEEECCCCCCEECCCEEEHHEEEEEEEEEEEHHHHHHHHHHH
RLLFIGSSCIYPRQCPQPIHEDYLLSGPLEKTNEPYALAKIAGVKLCESYNAQYGTSYFS
HHHHHCCCCCCCCCCCCCHHHCCEECCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHH
VMATNLYGPNDNYDPDNSHVLPSLIRKAHDAKVTGSHELVVWGSGAPRREFLYVDDMADA
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCEECCCCEEEEECCCCCCCEEEEECCHHHH
CVFLMERGLGDGLVNVGTGADLTIRELAEMVMDVVGFEGAIRFDSAKPDGTPRKLLNVDR
HHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHH
MTEMGWQAKTGLREGIAKAYQDYLQRFGSPR
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNSSRRMAIRPMTTTNNLPLDKDARIYVAGHRGMVGSAIERRLVAAGYSNVLARSHDELD
CCCCCCEEEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCHHHHHHCCCCCCC
LTNQADVFAFLREERPDFIFLAAGRVGGIHANNTYRADFIYRNLMIEANVIHGAWLAGVR
CCCCHHHHHHHHHCCCCEEEEECCCCCCEECCCEEEHHEEEEEEEEEEEHHHHHHHHHHH
RLLFIGSSCIYPRQCPQPIHEDYLLSGPLEKTNEPYALAKIAGVKLCESYNAQYGTSYFS
HHHHHCCCCCCCCCCCCCHHHCCEECCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHH
VMATNLYGPNDNYDPDNSHVLPSLIRKAHDAKVTGSHELVVWGSGAPRREFLYVDDMADA
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCEECCCCEEEEECCCCCCCEEEEECCHHHH
CVFLMERGLGDGLVNVGTGADLTIRELAEMVMDVVGFEGAIRFDSAKPDGTPRKLLNVDR
HHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHH
MTEMGWQAKTGLREGIAKAYQDYLQRFGSPR
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]