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The map label for this gene is sasR [H]

Identifier: 119899782

GI number: 119899782

Start: 3834272

End: 3835684

Strand: Reverse

Name: sasR [H]

Synonym: azo3493

Alternate gene names: 119899782

Gene position: 3835684-3834272 (Counterclockwise)

Preceding gene: 119899783

Following gene: 119899781

Centisome position: 87.65

GC content: 69.57

Gene sequence:

>1413_bases
ATGCAGCACGAGACTGCAGCGGCGGCAGCCGGCGGCGACAGCGCAGAATTCAACTGGCAGGCCCATTCCATCCTCATCGT
CGATGACGAGGAAGGCATGCGCAACTTCCTCGAACGCACGCTGAAACGGCGTTGCGGGCTGGTCGAGGCCGCCGCCGGCG
TCGAGCAGGCGGAGGCGCTGATGGCCCGGCTGCACTTCGACCTGCTGGTGCTGGACATCGCGCTGCCGGGCAAATCGGGC
GTGCAATGGCTGCACGAACTGCGCGCTCACGGCTTCGCCGGCGACGTGATCCTGATCACCGCGTTTGCCGACATGGACAC
CGCGATCGGCGCGCTGCGCGCGGGGGCCTCGGACTTCATCCTGAAGCCCTTCCGCGTCGATCAGATCCTCAACTCGATCA
AGCGCTGCTTCGAACGCGCCGGGCTGGCGCGCGAGAATTTCGTGCTGCGCCGCGAACTCGCCGAACGCTCCACCGATCTC
CTCGGCCTCATCGGCCACTCGCCCGCGATCCAGCAGTTGCGGGGCCTGATCCGGCGCGTCGGGCAGATGCCGAGCACGGT
GCTGCTGCTGGGCGAATCCGGCACCGGCAAGGAGGTGGCGGCGCGCGCGCTGCACCAGATGAGCCCGCGCGCGCACCGGC
CCTTCGTGCCGCTCAACTGTGCCGCGATCGCGTCCGAGCTGATCGAGTCCGAGCTGTTCGGCCACGTCAAGGGCGCCTTC
ACCGGGGCCACCGAAACGCGCAACGGGCTGTTCTACTACGCCCACGGCGGCACGCTCTTCCTCGACGAGATCAGCGAACT
CCCGCTGGCCATGCAGACCCGCCTGCTGCGCGTGCTGGAAGAGCGCAAGCTGCGGCCGGTGGGCACCGAACGCGAGGTGC
CGGTCGATGTGCGCATCATCGCTGCGTCCAATCGCGACCTCGCCGCCGAAGTCGCCGCCGGCCGCTTCCGCCAGGACCTC
TATTTCCGCCTCGCGGTGGTGGAAATGCGGGTACCGGCGCTGCGCGAGCGGGTGGAGGACATCCCGGACCTGGTGAGCCA
CTTCATGACGCTGCTGTCGATGCAGCTCGGGGTGGCGCCGCTCCCGCTGTCGCACGAGCTGGTGACCCGGCTGGCGGCCT
ATCCGTGGCCGGGCAACGTGCGCGAACTGCGCAACCTGGTCGAGCGTTCGCTGATCCTCGGCGCCTTTCCCGCTGCCACC
GGCGCCTCCGCGCGGCGCGAACCGTCGCCTGCGGCACCGGGCGAACTCGAGCTGACGCTGGAAGAGGTGGAGAAGCGCCA
CATCCTGCGCGTGGTCGAAGCCTGCGCCGGCAACAAGGCAGAGGCCGCGCGCCGGCTGGCGGTATCGCGCAAGACCCTGG
ACCGCAAGTTCGCCGAATGGGGTGAGGCCGCAACCCCCGGCGGCGGCGACTGA

Upstream 100 bases:

>100_bases
GTGCCGGTGCTGGTCCTGTTCGCGGTAGTGTTCCTGGTGGTGATGAAACCGTTCTAAGGCTGCGATCGGCAGCACGAGGG
CGGGGCGGCAAGGAGACAGC

Downstream 100 bases:

>100_bases
CCCGCCCGATGCGTCTGCCCCGCTTGCTGCGCCCGCTGCTCGACTACTTCGCCGGCTCGGTCCGCGCCAAGCTGCTGTTC
CTGGTGCTTGCCCCGTTGAT

Product: sigma-54 dependent response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MQHETAAAAAGGDSAEFNWQAHSILIVDDEEGMRNFLERTLKRRCGLVEAAAGVEQAEALMARLHFDLLVLDIALPGKSG
VQWLHELRAHGFAGDVILITAFADMDTAIGALRAGASDFILKPFRVDQILNSIKRCFERAGLARENFVLRRELAERSTDL
LGLIGHSPAIQQLRGLIRRVGQMPSTVLLLGESGTGKEVAARALHQMSPRAHRPFVPLNCAAIASELIESELFGHVKGAF
TGATETRNGLFYYAHGGTLFLDEISELPLAMQTRLLRVLEERKLRPVGTEREVPVDVRIIAASNRDLAAEVAAGRFRQDL
YFRLAVVEMRVPALRERVEDIPDLVSHFMTLLSMQLGVAPLPLSHELVTRLAAYPWPGNVRELRNLVERSLILGAFPAAT
GASARREPSPAAPGELELTLEEVEKRHILRVVEACAGNKAEAARRLAVSRKTLDRKFAEWGEAATPGGGD

Sequences:

>Translated_470_residues
MQHETAAAAAGGDSAEFNWQAHSILIVDDEEGMRNFLERTLKRRCGLVEAAAGVEQAEALMARLHFDLLVLDIALPGKSG
VQWLHELRAHGFAGDVILITAFADMDTAIGALRAGASDFILKPFRVDQILNSIKRCFERAGLARENFVLRRELAERSTDL
LGLIGHSPAIQQLRGLIRRVGQMPSTVLLLGESGTGKEVAARALHQMSPRAHRPFVPLNCAAIASELIESELFGHVKGAF
TGATETRNGLFYYAHGGTLFLDEISELPLAMQTRLLRVLEERKLRPVGTEREVPVDVRIIAASNRDLAAEVAAGRFRQDL
YFRLAVVEMRVPALRERVEDIPDLVSHFMTLLSMQLGVAPLPLSHELVTRLAAYPWPGNVRELRNLVERSLILGAFPAAT
GASARREPSPAAPGELELTLEEVEKRHILRVVEACAGNKAEAARRLAVSRKTLDRKFAEWGEAATPGGGD
>Mature_470_residues
MQHETAAAAAGGDSAEFNWQAHSILIVDDEEGMRNFLERTLKRRCGLVEAAAGVEQAEALMARLHFDLLVLDIALPGKSG
VQWLHELRAHGFAGDVILITAFADMDTAIGALRAGASDFILKPFRVDQILNSIKRCFERAGLARENFVLRRELAERSTDL
LGLIGHSPAIQQLRGLIRRVGQMPSTVLLLGESGTGKEVAARALHQMSPRAHRPFVPLNCAAIASELIESELFGHVKGAF
TGATETRNGLFYYAHGGTLFLDEISELPLAMQTRLLRVLEERKLRPVGTEREVPVDVRIIAASNRDLAAEVAAGRFRQDL
YFRLAVVEMRVPALRERVEDIPDLVSHFMTLLSMQLGVAPLPLSHELVTRLAAYPWPGNVRELRNLVERSLILGAFPAAT
GASARREPSPAAPGELELTLEEVEKRHILRVVEACAGNKAEAARRLAVSRKTLDRKFAEWGEAATPGGGD

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=450, Percent_Identity=39.1111111111111, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1790437, Length=444, Percent_Identity=41.4414414414414, Blast_Score=311, Evalue=6e-86,
Organism=Escherichia coli, GI1790299, Length=474, Percent_Identity=38.8185654008439, Blast_Score=279, Evalue=4e-76,
Organism=Escherichia coli, GI1788905, Length=427, Percent_Identity=36.768149882904, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI1789233, Length=319, Percent_Identity=40.4388714733542, Blast_Score=237, Evalue=1e-63,
Organism=Escherichia coli, GI87082117, Length=347, Percent_Identity=41.7867435158501, Blast_Score=237, Evalue=2e-63,
Organism=Escherichia coli, GI1789087, Length=337, Percent_Identity=41.2462908011869, Blast_Score=221, Evalue=7e-59,
Organism=Escherichia coli, GI87082152, Length=309, Percent_Identity=42.3948220064725, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1786524, Length=334, Percent_Identity=41.3173652694611, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1787583, Length=317, Percent_Identity=35.9621451104101, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI87081872, Length=231, Percent_Identity=41.991341991342, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789828, Length=212, Percent_Identity=41.0377358490566, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI87081858, Length=320, Percent_Identity=29.375, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1789809, Length=120, Percent_Identity=35, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1786838, Length=122, Percent_Identity=29.5081967213115, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51503; Mature: 51503

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHETAAAAAGGDSAEFNWQAHSILIVDDEEGMRNFLERTLKRRCGLVEAAAGVEQAEAL
CCCCHHHHHCCCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHH
MARLHFDLLVLDIALPGKSGVQWLHELRAHGFAGDVILITAFADMDTAIGALRAGASDFI
HHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHCCCHHHH
LKPFRVDQILNSIKRCFERAGLARENFVLRRELAERSTDLLGLIGHSPAIQQLRGLIRRV
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GQMPSTVLLLGESGTGKEVAARALHQMSPRAHRPFVPLNCAAIASELIESELFGHVKGAF
CCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
TGATETRNGLFYYAHGGTLFLDEISELPLAMQTRLLRVLEERKLRPVGTEREVPVDVRII
CCCCCCCCCEEEEECCCEEEEHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
AASNRDLAAEVAAGRFRQDLYFRLAVVEMRVPALRERVEDIPDLVSHFMTLLSMQLGVAP
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPLSHELVTRLAAYPWPGNVRELRNLVERSLILGAFPAATGASARREPSPAAPGELELTL
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEH
EEVEKRHILRVVEACAGNKAEAARRLAVSRKTLDRKFAEWGEAATPGGGD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQHETAAAAAGGDSAEFNWQAHSILIVDDEEGMRNFLERTLKRRCGLVEAAAGVEQAEAL
CCCCHHHHHCCCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHH
MARLHFDLLVLDIALPGKSGVQWLHELRAHGFAGDVILITAFADMDTAIGALRAGASDFI
HHHHHHHHHEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHCCCHHHH
LKPFRVDQILNSIKRCFERAGLARENFVLRRELAERSTDLLGLIGHSPAIQQLRGLIRRV
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
GQMPSTVLLLGESGTGKEVAARALHQMSPRAHRPFVPLNCAAIASELIESELFGHVKGAF
CCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
TGATETRNGLFYYAHGGTLFLDEISELPLAMQTRLLRVLEERKLRPVGTEREVPVDVRII
CCCCCCCCCEEEEECCCEEEEHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
AASNRDLAAEVAAGRFRQDLYFRLAVVEMRVPALRERVEDIPDLVSHFMTLLSMQLGVAP
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LPLSHELVTRLAAYPWPGNVRELRNLVERSLILGAFPAATGASARREPSPAAPGELELTL
CCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEH
EEVEKRHILRVVEACAGNKAEAARRLAVSRKTLDRKFAEWGEAATPGGGD
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]