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The map label for this gene is livM4 [H]

Identifier: 119899733

GI number: 119899733

Start: 3777218

End: 3778285

Strand: Reverse

Name: livM4 [H]

Synonym: azo3444

Alternate gene names: 119899733

Gene position: 3778285-3777218 (Counterclockwise)

Preceding gene: 119899734

Following gene: 119899732

Centisome position: 86.34

GC content: 64.79

Gene sequence:

>1068_bases
ATGCTTTACCGTGAGGCCGGCCAGTTCAAGGCCAGTTACCAGGAAGACCAGCAGATTTTCCCGATCCGCCAGGACCGGAT
CGGCTTCGCGGCCATCCTGGTGTTCTTCTTCGGCGTGGTGCCGCTCGTCGCCAACCAGTACTGGCTGCAGGCCATCCTGA
TCCCGGTGCTGATCTTCTCGCTCGCTGCGATCGGGCTGAACATCCTCACCGGCTACGCCGGCCAGCTGTCGCTCGGGTCG
GCCGCCTTCATGGCGGTGGGAGCCTTCGGCGCCTACAACTTCATGTTGCGTATCGACGGCATGCCCTTCCTGGTGGGCAT
CGTGCTGGGCGGGCTGTGCGCGGCCGCGGTCGGGGTGGCCTTCGGGCTGCCGTCCCTGCGCATCAAGGGCTTCTACCTGG
CGGTGGCGACGCTGGCGGCGCAGTTTTTCATCGTCTGGGCGCTGGTCAAGTTTCCGTGGTTCTCCAACCATGCCAGCTCG
GGCGTGATCACCGCGCAGCGGGTCGAGATCCTCGGCTATGCCTTCGACAGCCCGGTGAGCAAATACCTGCTGACGCTCGC
GGTGGTGGCGGTGCTGGCGCTGCTCGCCAAGAACCTCGTGCGCAGCACCACCGGCCGCAGCTGGATGGCGGTGCGCGACA
TGGACGTCGCCGCCGAAGTGATCGGCATCCGCCCGATGCACGCCAAGCTCACGGCCTTCGCGGTGTCGTCCTTCTACTGC
GGCGTGGCCGGTGCGCTGTATGCCTACACCTACCTCGGCACCGTGGAGCCGGAAGGCTTCAGCCTCGACCTGTCGTTCAA
GATCCTGTTCATGATCATCATCGGCGGCGTCGGTTCGATCATGGGTTCCTTCTTCGGCGCGGCCTTCATCGTGCTGATGC
CGATCTTCCTCAACGTCGTCATGCACTCGGTGTTCGGCGGGGCGATGCCCGCCGGCATGGGTTCCAACCTCGAACTGATC
ATTTTCGGCGGGCTGATCATCTTCTTCCTGATCGTCGAGCCGCACGGCCTCGCCCGCCTGTGGCAGCTCGGCAAGGAAAA
GCTGCGGCTGTGGCCCTTCCCGCACTGA

Upstream 100 bases:

>100_bases
GCGCGGCGCGCCCGCCGGAACGGAGCGGCAATGCGCAGCCGATCCTTCAGGCCTGCCCCCGGGCAAGGCCAAGGCGCTAG
AAAACGATTGGAGACAACCC

Downstream 100 bases:

>100_bases
GCACGTCCGCACACGCGGCCGCCGTCACGGCGGCGGCCCGGCACCGCGCAACACGCAGCACCACACACACAAGGAGGAGA
CAAGTCATGCAACTCAAGAA

Product: putative branched-chain amino acid transport permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MLYREAGQFKASYQEDQQIFPIRQDRIGFAAILVFFFGVVPLVANQYWLQAILIPVLIFSLAAIGLNILTGYAGQLSLGS
AAFMAVGAFGAYNFMLRIDGMPFLVGIVLGGLCAAAVGVAFGLPSLRIKGFYLAVATLAAQFFIVWALVKFPWFSNHASS
GVITAQRVEILGYAFDSPVSKYLLTLAVVAVLALLAKNLVRSTTGRSWMAVRDMDVAAEVIGIRPMHAKLTAFAVSSFYC
GVAGALYAYTYLGTVEPEGFSLDLSFKILFMIIIGGVGSIMGSFFGAAFIVLMPIFLNVVMHSVFGGAMPAGMGSNLELI
IFGGLIIFFLIVEPHGLARLWQLGKEKLRLWPFPH

Sequences:

>Translated_355_residues
MLYREAGQFKASYQEDQQIFPIRQDRIGFAAILVFFFGVVPLVANQYWLQAILIPVLIFSLAAIGLNILTGYAGQLSLGS
AAFMAVGAFGAYNFMLRIDGMPFLVGIVLGGLCAAAVGVAFGLPSLRIKGFYLAVATLAAQFFIVWALVKFPWFSNHASS
GVITAQRVEILGYAFDSPVSKYLLTLAVVAVLALLAKNLVRSTTGRSWMAVRDMDVAAEVIGIRPMHAKLTAFAVSSFYC
GVAGALYAYTYLGTVEPEGFSLDLSFKILFMIIIGGVGSIMGSFFGAAFIVLMPIFLNVVMHSVFGGAMPAGMGSNLELI
IFGGLIIFFLIVEPHGLARLWQLGKEKLRLWPFPH
>Mature_355_residues
MLYREAGQFKASYQEDQQIFPIRQDRIGFAAILVFFFGVVPLVANQYWLQAILIPVLIFSLAAIGLNILTGYAGQLSLGS
AAFMAVGAFGAYNFMLRIDGMPFLVGIVLGGLCAAAVGVAFGLPSLRIKGFYLAVATLAAQFFIVWALVKFPWFSNHASS
GVITAQRVEILGYAFDSPVSKYLLTLAVVAVLALLAKNLVRSTTGRSWMAVRDMDVAAEVIGIRPMHAKLTAFAVSSFYC
GVAGALYAYTYLGTVEPEGFSLDLSFKILFMIIIGGVGSIMGSFFGAAFIVLMPIFLNVVMHSVFGGAMPAGMGSNLELI
IFGGLIIFFLIVEPHGLARLWQLGKEKLRLWPFPH

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=305, Percent_Identity=26.8852459016393, Blast_Score=95, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 38400; Mature: 38400

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYREAGQFKASYQEDQQIFPIRQDRIGFAAILVFFFGVVPLVANQYWLQAILIPVLIFS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAIGLNILTGYAGQLSLGSAAFMAVGAFGAYNFMLRIDGMPFLVGIVLGGLCAAAVGVA
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHH
FGLPSLRIKGFYLAVATLAAQFFIVWALVKFPWFSNHASSGVITAQRVEILGYAFDSPVS
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHCCHHH
KYLLTLAVVAVLALLAKNLVRSTTGRSWMAVRDMDVAAEVIGIRPMHAKLTAFAVSSFYC
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GVAGALYAYTYLGTVEPEGFSLDLSFKILFMIIIGGVGSIMGSFFGAAFIVLMPIFLNVV
HHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MHSVFGGAMPAGMGSNLELIIFGGLIIFFLIVEPHGLARLWQLGKEKLRLWPFPH
HHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHCHHHCCCCCCCC
>Mature Secondary Structure
MLYREAGQFKASYQEDQQIFPIRQDRIGFAAILVFFFGVVPLVANQYWLQAILIPVLIFS
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAAIGLNILTGYAGQLSLGSAAFMAVGAFGAYNFMLRIDGMPFLVGIVLGGLCAAAVGVA
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHHHH
FGLPSLRIKGFYLAVATLAAQFFIVWALVKFPWFSNHASSGVITAQRVEILGYAFDSPVS
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHCCHHH
KYLLTLAVVAVLALLAKNLVRSTTGRSWMAVRDMDVAAEVIGIRPMHAKLTAFAVSSFYC
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GVAGALYAYTYLGTVEPEGFSLDLSFKILFMIIIGGVGSIMGSFFGAAFIVLMPIFLNVV
HHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MHSVFGGAMPAGMGSNLELIIFGGLIIFFLIVEPHGLARLWQLGKEKLRLWPFPH
HHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]