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The map label for this gene is tufA [H]

Identifier: 119899708

GI number: 119899708

Start: 3742952

End: 3744142

Strand: Reverse

Name: tufA [H]

Synonym: azo3419

Alternate gene names: 119899708

Gene position: 3744142-3742952 (Counterclockwise)

Preceding gene: 119899709

Following gene: 119899707

Centisome position: 85.56

GC content: 62.55

Gene sequence:

>1191_bases
ATGGCTAAGGGTAAGTTCGAGCGGACGAAACCGCACGTAAACGTTGGTACGATCGGTCACGTTGACCACGGCAAGACGAC
GCTGACGGCGGCGATCACCACGATTCTGTCGAAGAAGTTCGGCGGTGAAGCGAAGGCCTACGACCAGATCGACGCGGCGC
CGGAAGAAAAGGCGCGTGGTATCACGATCAACACCGCGCACGTCGAGTACGAAACCGCGACCCGTCACTACGCCCACGTC
GATTGCCCGGGCCACGCCGACTACGTCAAGAACATGATCACCGGCGCTGCGCAGATGGACGGCGCGATCCTGGTGTGTTC
GGCCGCGGACGGCCCGATGCCGCAGACCCGCGAGCACATCCTGCTGGCGCGTCAGGTTGGTGTGCCGTACATCATCGTCT
TCCTGAACAAGTGCGACATGGTCGACGACGAAGAGCTGCTCGAGCTGGTCGAAATGGAAGTGCGCGAACTGCTCTCCAAG
TACGACTTCCCGGGCGATGACATTCCCATCGTCAAGGGTTCGGCGCTGAAGGCGCTCGAAGGCGACCAGTCCGACATCGG
CGAGCCGGCGATCTTCCGCCTGGCCGACGCGCTGGACAGCTACATCCCGACCCCGGAGCGTGCGATCGACAAGCCCTTCC
TGCTGCCGATCGAAGACGTGTTCTCGATCTCCGGCCGCGGCACCGTGGTGACCGGTCGTGTCGAGCGCGGCATCGTGAAG
GTTGGCGAAGAAATCGAAATCGTCGGCATCAAGCCCACCGTCAAGACCACCTGCACCGGCGTGGAAATGTTCCGCAAGCT
GCTGGACCAGGGTCAGGCGGGCGACAACGTCGGCGTGCTGCTGCGCGGCACCAAGCGTGAAGACGTCGAGCGTGGCCAGG
TGCTGTGCAAGCCGGGTTCGATCACCCCGCACACCCACTTCACCGGCGAAATCTACGTGCTGTCGAAGGAAGAGGGCGGT
CGTCACACCCCGTTCTTCAACAACTACCGTCCGCAGTTCTACTTCCGTACCACCGACGTGACCGGTTCGATCTCGCTGCC
CGAAGGCACCGAGATGGTCATGCCGGGCGACAACGTGTCGATCACCGTGAAGCTGATCGCCCCGATCGCCATGGAAGAAG
GTCTGCGTTTCGCCATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTCGTTGCCAAGATCATCGAGTAA

Upstream 100 bases:

>100_bases
CTCCATGGAATTCAAGCACTACTCCGAAGCGCCGAAGAGCGTCGCGGAGGCGGTGATCAGCAATCGCAAGTAATCAACCG
AAACCTCACGAGGACAAGCA

Downstream 100 bases:

>100_bases
TATCGTTTCAAGGGCGGGCCATCGTGGCCCGCCCTCTCGCTCTTTGGGAATTTCCTCATGCAAAACCAGAAGATCCGTAT
CCGCCTGAAGGCTTTCGACT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTILSKKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETATRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSK
YDFPGDDIPIVKGSALKALEGDQSDIGEPAIFRLADALDSYIPTPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIVK
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLCKPGSITPHTHFTGEIYVLSKEEGG
RHTPFFNNYRPQFYFRTTDVTGSISLPEGTEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE

Sequences:

>Translated_396_residues
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTILSKKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETATRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSK
YDFPGDDIPIVKGSALKALEGDQSDIGEPAIFRLADALDSYIPTPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIVK
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLCKPGSITPHTHFTGEIYVLSKEEGG
RHTPFFNNYRPQFYFRTTDVTGSISLPEGTEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
>Mature_395_residues
AKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTILSKKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETATRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSKY
DFPGDDIPIVKGSALKALEGDQSDIGEPAIFRLADALDSYIPTPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIVKV
GEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLCKPGSITPHTHFTGEIYVLSKEEGGR
HTPFFNNYRPQFYFRTTDVTGSISLPEGTEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=56.5989847715736, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI223555963, Length=440, Percent_Identity=31.1363636363636, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI5729864, Length=440, Percent_Identity=31.1363636363636, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=28.7946428571429, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=29.0178571428571, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI46094014, Length=435, Percent_Identity=29.8850574712644, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI194018522, Length=434, Percent_Identity=29.2626728110599, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI194018520, Length=434, Percent_Identity=29.2626728110599, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI194097354, Length=434, Percent_Identity=29.2626728110599, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI54607086, Length=319, Percent_Identity=27.2727272727273, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI4503507, Length=276, Percent_Identity=27.8985507246377, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=31.1111111111111, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=83.0379746835443, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=83.0379746835443, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=378, Percent_Identity=29.6296296296296, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI48994988, Length=275, Percent_Identity=31.6363636363636, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1789108, Length=319, Percent_Identity=29.4670846394984, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17556456, Length=403, Percent_Identity=53.1017369727047, Blast_Score=413, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=45.7364341085271, Blast_Score=341, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=450, Percent_Identity=30.6666666666667, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17569207, Length=450, Percent_Identity=30.6666666666667, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI32566303, Length=431, Percent_Identity=28.538283062645, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=438, Percent_Identity=28.310502283105, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=435, Percent_Identity=28.2758620689655, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=352, Percent_Identity=29.8295454545455, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=301, Percent_Identity=31.2292358803987, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=36.9127516778523, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=295, Percent_Identity=26.7796610169492, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=238, Percent_Identity=27.3109243697479, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=66.1616161616162, Blast_Score=538, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=29.9319727891156, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=29.9319727891156, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6320377, Length=343, Percent_Identity=30.0291545189504, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322937, Length=331, Percent_Identity=26.5861027190332, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=265, Percent_Identity=27.5471698113208, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=27.3109243697479, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323098, Length=192, Percent_Identity=28.6458333333333, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=59.0330788804071, Blast_Score=497, Evalue=1e-141,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=59.0330788804071, Blast_Score=497, Evalue=1e-141,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=53.6269430051813, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI45553807, Length=449, Percent_Identity=29.6213808463252, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI45553816, Length=449, Percent_Identity=29.6213808463252, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24651721, Length=449, Percent_Identity=29.6213808463252, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI17864154, Length=449, Percent_Identity=29.6213808463252, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=29.2410714285714, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=29.2410714285714, Blast_Score=162, Evalue=4e-40,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=28.8990825688073, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17137380, Length=321, Percent_Identity=31.7757009345794, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI19922690, Length=307, Percent_Identity=29.9674267100977, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=27.5092936802974, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=27.5092936802974, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28572034, Length=165, Percent_Identity=36.3636363636364, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43117; Mature: 42986

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTILSKKFGGEAKAYDQIDAAPEEKARG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHCCC
ITINTAHVEYETATRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSKYDFPGDDIPIVKGSALKALE
HEEHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHCC
GDQSDIGEPAIFRLADALDSYIPTPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIVK
CCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHHHCCCCCCEEEECHHHHCHHC
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLCKPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCC
ITPHTHFTGEIYVLSKEEGGRHTPFFNNYRPQFYFRTTDVTGSISLPEGTEMVMPGDNVS
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCE
ITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
EEEEEEECHHHHCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTILSKKFGGEAKAYDQIDAAPEEKARG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHCCC
ITINTAHVEYETATRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSKYDFPGDDIPIVKGSALKALE
HEEHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHCC
GDQSDIGEPAIFRLADALDSYIPTPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIVK
CCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCEEEHHHHHCCCCCCEEEECHHHHCHHC
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGVLLRGTKREDVERGQVLCKPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCC
ITPHTHFTGEIYVLSKEEGGRHTPFFNNYRPQFYFRTTDVTGSISLPEGTEMVMPGDNVS
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCEEEECCCCCE
ITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
EEEEEEECHHHHCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA