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The map label for this gene is nolF [H]

Identifier: 119899665

GI number: 119899665

Start: 3710800

End: 3711864

Strand: Reverse

Name: nolF [H]

Synonym: azo3376

Alternate gene names: 119899665

Gene position: 3711864-3710800 (Counterclockwise)

Preceding gene: 119899666

Following gene: 119899664

Centisome position: 84.82

GC content: 73.71

Gene sequence:

>1065_bases
ATGATGATCCCGCGCCCGCTTGTCCTGCTGCTGTCCCTGCTGATTGCCACCTCGTCCGCGCATTCCGCTGATGAGGTAGA
GGCCATTCCGACCGTGCGCATCGGCGCGCGTGCGCTCGCCAGCGCAATCGTCGCGGAGGCGAGTATCGAGGCGGTCCGGC
AGAGCACGCTGGCAGCGCAGGTGCCGGGGCGCGTGGTGCAGCTGGCCGCGGATGCCGGCGATGCGGTCCGCGCCGGCCAG
GTGCTGCTGCGCATCGACGCGGCGGAAGCCAGCCAGGCGGTCGCCGCGGCCGACGCCGACGTGGCACGCGCCGAGGCCGG
GTTGACCGAGGCGCGCGCCAACCTTGCCCGTAGCCGGAGCCTGTTCGAGCGCAAATTCGTGAGCCAGGCCGTGCTCGACC
AGGCCCAGGCGGCCTTCGATGCCGCTGCCGCGCAGCTGCGCGCCGCGCGTGCGGGCCGCGGGCAGGCGGCAAGCGTCCAG
GGCCACGCCGCGGTGGCTGCGCCGTTCGCCGGCGTGATCGCCGCGCGCTACATCGAGGCCGGGGAAATGGCCCAGCCCGG
GCGCGAATTGCTCACCCTGTACGACCCCGCAGCCTTGCGCGCCGTGGTCGATCTGCCGCAGCAACGCCTCGCCCAGCTCG
GCACCGGCCCCGTGAAGGCCCGCATCGAGCTGCCGGGCCAGCAGCGGGTGTTCGAGGCCGCGAGCGTCACCGTGCTGCCG
GCCGCAGACGCGCGCACCCACACCGTGCGGGTGCGGGTGGAGCTGCCGCCGGGCAGTAATGACGTGGTTCCGGGCACCTA
TGCCCGCGTCCATTTCCTCTCGGCTGCAACCCGCCGTCTCACCGTGCCTGCCGCCGCCGTGCTGCGCAGGGGCGAGCTGA
CCGCGGTCTATGTGGCGGAAGGGCGGGGCGGCTTCAGCCTGCGCCAGCTTCGCCTGGGCGATACGATCAGCGACGACACC
GGACGTCCCGCCGTCGAGGTGCTCGCCGGCCTGGGCGAGGGCGATGTGGTGGCGCTCGATCCGGTGCAGGCCGGCATCGT
CGCCCGCGCGCGGCAGGGCAAGTGA

Upstream 100 bases:

>100_bases
GCCGACGCCGTCATCGACGCGATCGCGGCCTACGCGGAGCGCTGCCGGGGCTGAACGCAGCTGTCTTGAAACTTGTGCAT
ATAAGAATATACTTATATGC

Downstream 100 bases:

>100_bases
TGGCTGCGGAGCAAGGCCTTGCAGGGCGCCTCGCCGCGGCGTTCCAGCGCAACGCGATCACGCCGCTGATCGCGCTGGTG
CTGATGCTGGCCGGCGTGTT

Product: membrane fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MMIPRPLVLLLSLLIATSSAHSADEVEAIPTVRIGARALASAIVAEASIEAVRQSTLAAQVPGRVVQLAADAGDAVRAGQ
VLLRIDAAEASQAVAAADADVARAEAGLTEARANLARSRSLFERKFVSQAVLDQAQAAFDAAAAQLRAARAGRGQAASVQ
GHAAVAAPFAGVIAARYIEAGEMAQPGRELLTLYDPAALRAVVDLPQQRLAQLGTGPVKARIELPGQQRVFEAASVTVLP
AADARTHTVRVRVELPPGSNDVVPGTYARVHFLSAATRRLTVPAAAVLRRGELTAVYVAEGRGGFSLRQLRLGDTISDDT
GRPAVEVLAGLGEGDVVALDPVQAGIVARARQGK

Sequences:

>Translated_354_residues
MMIPRPLVLLLSLLIATSSAHSADEVEAIPTVRIGARALASAIVAEASIEAVRQSTLAAQVPGRVVQLAADAGDAVRAGQ
VLLRIDAAEASQAVAAADADVARAEAGLTEARANLARSRSLFERKFVSQAVLDQAQAAFDAAAAQLRAARAGRGQAASVQ
GHAAVAAPFAGVIAARYIEAGEMAQPGRELLTLYDPAALRAVVDLPQQRLAQLGTGPVKARIELPGQQRVFEAASVTVLP
AADARTHTVRVRVELPPGSNDVVPGTYARVHFLSAATRRLTVPAAAVLRRGELTAVYVAEGRGGFSLRQLRLGDTISDDT
GRPAVEVLAGLGEGDVVALDPVQAGIVARARQGK
>Mature_354_residues
MMIPRPLVLLLSLLIATSSAHSADEVEAIPTVRIGARALASAIVAEASIEAVRQSTLAAQVPGRVVQLAADAGDAVRAGQ
VLLRIDAAEASQAVAAADADVARAEAGLTEARANLARSRSLFERKFVSQAVLDQAQAAFDAAAAQLRAARAGRGQAASVQ
GHAAVAAPFAGVIAARYIEAGEMAQPGRELLTLYDPAALRAVVDLPQQRLAQLGTGPVKARIELPGQQRVFEAASVTVLP
AADARTHTVRVRVELPPGSNDVVPGTYARVHFLSAATRRLTVPAAAVLRRGELTAVYVAEGRGGFSLRQLRLGDTISDDT
GRPAVEVLAGLGEGDVVALDPVQAGIVARARQGK

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=336, Percent_Identity=25.8928571428571, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789929, Length=314, Percent_Identity=26.1146496815287, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI87082036, Length=306, Percent_Identity=23.8562091503268, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 36596; Mature: 36596

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIPRPLVLLLSLLIATSSAHSADEVEAIPTVRIGARALASAIVAEASIEAVRQSTLAAQ
CCCCCHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPGRVVQLAADAGDAVRAGQVLLRIDAAEASQAVAAADADVARAEAGLTEARANLARSRS
CCCCEEEEECCCCCHHHHCEEEEEEECHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHH
LFERKFVSQAVLDQAQAAFDAAAAQLRAARAGRGQAASVQGHAAVAAPFAGVIAARYIEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
GEMAQPGRELLTLYDPAALRAVVDLPQQRLAQLGTGPVKARIELPGQQRVFEAASVTVLP
CCCCCCCHHHEEHCCHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCHHHHHHHHCEEEEE
AADARTHTVRVRVELPPGSNDVVPGTYARVHFLSAATRRLTVPAAAVLRRGELTAVYVAE
CCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHEECCHHHHHCCCCEEEEEEEC
GRGGFSLRQLRLGDTISDDTGRPAVEVLAGLGEGDVVALDPVQAGIVARARQGK
CCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEEECCHHHHHHHHCCCCC
>Mature Secondary Structure
MMIPRPLVLLLSLLIATSSAHSADEVEAIPTVRIGARALASAIVAEASIEAVRQSTLAAQ
CCCCCHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPGRVVQLAADAGDAVRAGQVLLRIDAAEASQAVAAADADVARAEAGLTEARANLARSRS
CCCCEEEEECCCCCHHHHCEEEEEEECHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHH
LFERKFVSQAVLDQAQAAFDAAAAQLRAARAGRGQAASVQGHAAVAAPFAGVIAARYIEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
GEMAQPGRELLTLYDPAALRAVVDLPQQRLAQLGTGPVKARIELPGQQRVFEAASVTVLP
CCCCCCCHHHEEHCCHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCHHHHHHHHCEEEEE
AADARTHTVRVRVELPPGSNDVVPGTYARVHFLSAATRRLTVPAAAVLRRGELTAVYVAE
CCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHEECCHHHHHCCCCEEEEEEEC
GRGGFSLRQLRLGDTISDDTGRPAVEVLAGLGEGDVVALDPVQAGIVARARQGK
CCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEEECCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]