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The map label for this gene is cfaA [H]

Identifier: 119899600

GI number: 119899600

Start: 3648753

End: 3650036

Strand: Direct

Name: cfaA [H]

Synonym: azo3311

Alternate gene names: 119899600

Gene position: 3648753-3650036 (Clockwise)

Preceding gene: 119899599

Following gene: 119899601

Centisome position: 83.38

GC content: 71.81

Gene sequence:

>1284_bases
ATGAATGCCGCCGAATCGCAACTCCCCCGCCCCGGCGTCCTGGAGCGCCCGCCCGCGGGCGCGGCGGGGCGCACCGGGCA
AGACGCCGCACTGCCCCGTGGCGCGCGCCTCGCGCTGGACATGCTGGACCGCATCGAAGGCGGCAGCGTGGCGCTGACCC
TGCCCGACGGCGGCACCTACTGCCTCGGCCGCGGGCCGCAACGCGCCCACCTGCGGGTGCGCGACCTCGGGATGTTCGAC
GCCGCGCTGGCCCACGGCGACATCGGCTTCGGCGAGGGCTACATGGAAGGCGACTGGGACTGCGCGGATGGCGACGAACT
GGCCGCGCTGCTCGGCCTGCTCGCCACCAACCGCAAGGTGCTGGACACCGCCATCCGCGGTCGTTTCCTGCGCCTGCTCG
GCCACCGCCTGGCCCATCTGTTCAATGCCAACACCCGCAGCGGCGCGCGGCGCAACATCGAGGCCCACTACGACCTCGGC
AACCGCTTCTACCGGCTGTGGCTGGACCCCACGATGTCCTACTCGGCCGCGCTGTTCGCCGAGGGCGACGACCAGCCCGA
TGCCGACCGTCTGGCCGAGGCCCAGCGGCGCAAGTACCGCCGCGTGCTCGACCAGCTCGGCGCCCGCGCGGGACAGACCG
TGCTCGAGATCGGCTGCGGCTGGGGCGGCTTCGCCGAGGTGGCGGTGCAGGAGTATGGCTGCCGCGTGGTCGGCCTCACG
CTGTCGCCCGCCCAGCTTGGCTATGCGCAGGCGCGCGCGGCCGCGGGCGGTTACGCCACCGAGGCGGAATTCCGGCTGTG
CGACTACCGCGATGTGGAGGGGCGCTTCGACCACATCGCCTCGATCGAGATGATCGAGGCGGTCGGCGAGGCTTACTGGC
CCACCTATTTCCGCCAGATCGCCCGCCTGCTTGCGTCCGGCGGCCGCTGCGTGGTGCAGGCGATCACGATCGACGACGCG
TTGTTCGCGCGCTATCGCCGCGGCACCGACTTCATCCAGCGCTACATCTTTCCGGGCGGCATGCTGCCGGCGCCTGCGCA
GATCCGCCGCCACAGTGCGCGCGCCGGCCTCGCTATCACCGACGACTTCGGCTTCGGCGACCACTACGCCCGCACGCTGG
CGTGCTGGCGGCAGCGCTTTGACGCGCAGGGCGAAGCCCTGGCACGGCTAGGCTTTCCGGCGCGCTTCACGCGCATGTGG
CGCTTCTATCTCGCCTACTGCGAAGCCGGCTTTCGCAGTGGCGACCTCGACGTGCGCCACTACACCTTCTCGCGGCGGGG
CTGA

Upstream 100 bases:

>100_bases
TCGCCGTCCTTGCCCGCATCCATTGGCAGGCGCTGCGCCTGATCGTCAAAGGCGCCGCCTTCCACCGCAAGCCCCAGCCC
CCACTCGAGGAGACCAGCCG

Downstream 100 bases:

>100_bases
CCATGTGGCCCGCGGTTCGCCTCTGCCTGACGCCGACCGGCCCACTGCGGCGCGCGCTGGCCGGCATCGTGCTGGCGGGA
TGCGCCGCCGCGAGCCTGGC

Product: cyclopropane-fatty-acyl-phospholipid synthase

Products: NA

Alternate protein names: CFA synthase; Cyclopropane fatty acid synthase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNAAESQLPRPGVLERPPAGAAGRTGQDAALPRGARLALDMLDRIEGGSVALTLPDGGTYCLGRGPQRAHLRVRDLGMFD
AALAHGDIGFGEGYMEGDWDCADGDELAALLGLLATNRKVLDTAIRGRFLRLLGHRLAHLFNANTRSGARRNIEAHYDLG
NRFYRLWLDPTMSYSAALFAEGDDQPDADRLAEAQRRKYRRVLDQLGARAGQTVLEIGCGWGGFAEVAVQEYGCRVVGLT
LSPAQLGYAQARAAAGGYATEAEFRLCDYRDVEGRFDHIASIEMIEAVGEAYWPTYFRQIARLLASGGRCVVQAITIDDA
LFARYRRGTDFIQRYIFPGGMLPAPAQIRRHSARAGLAITDDFGFGDHYARTLACWRQRFDAQGEALARLGFPARFTRMW
RFYLAYCEAGFRSGDLDVRHYTFSRRG

Sequences:

>Translated_427_residues
MNAAESQLPRPGVLERPPAGAAGRTGQDAALPRGARLALDMLDRIEGGSVALTLPDGGTYCLGRGPQRAHLRVRDLGMFD
AALAHGDIGFGEGYMEGDWDCADGDELAALLGLLATNRKVLDTAIRGRFLRLLGHRLAHLFNANTRSGARRNIEAHYDLG
NRFYRLWLDPTMSYSAALFAEGDDQPDADRLAEAQRRKYRRVLDQLGARAGQTVLEIGCGWGGFAEVAVQEYGCRVVGLT
LSPAQLGYAQARAAAGGYATEAEFRLCDYRDVEGRFDHIASIEMIEAVGEAYWPTYFRQIARLLASGGRCVVQAITIDDA
LFARYRRGTDFIQRYIFPGGMLPAPAQIRRHSARAGLAITDDFGFGDHYARTLACWRQRFDAQGEALARLGFPARFTRMW
RFYLAYCEAGFRSGDLDVRHYTFSRRG
>Mature_427_residues
MNAAESQLPRPGVLERPPAGAAGRTGQDAALPRGARLALDMLDRIEGGSVALTLPDGGTYCLGRGPQRAHLRVRDLGMFD
AALAHGDIGFGEGYMEGDWDCADGDELAALLGLLATNRKVLDTAIRGRFLRLLGHRLAHLFNANTRSGARRNIEAHYDLG
NRFYRLWLDPTMSYSAALFAEGDDQPDADRLAEAQRRKYRRVLDQLGARAGQTVLEIGCGWGGFAEVAVQEYGCRVVGLT
LSPAQLGYAQARAAAGGYATEAEFRLCDYRDVEGRFDHIASIEMIEAVGEAYWPTYFRQIARLLASGGRCVVQAITIDDA
LFARYRRGTDFIQRYIFPGGMLPAPAQIRRHSARAGLAITDDFGFGDHYARTLACWRQRFDAQGEALARLGFPARFTRMW
RFYLAYCEAGFRSGDLDVRHYTFSRRG

Specific function: Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge [H]

COG id: COG2230

COG function: function code M; Cyclopropane fatty acid synthase and related methyltransferases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CFA/CMAS family [H]

Homologues:

Organism=Escherichia coli, GI1787951, Length=367, Percent_Identity=34.0599455040872, Blast_Score=185, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI133904226, Length=364, Percent_Identity=38.1868131868132, Blast_Score=247, Evalue=7e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003333 [H]

Pfam domain/function: PF02353 CMAS [H]

EC number: =2.1.1.79 [H]

Molecular weight: Translated: 47205; Mature: 47205

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAAESQLPRPGVLERPPAGAAGRTGQDAALPRGARLALDMLDRIEGGSVALTLPDGGTY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCE
CLGRGPQRAHLRVRDLGMFDAALAHGDIGFGEGYMEGDWDCADGDELAALLGLLATNRKV
ECCCCCCHHEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHH
LDTAIRGRFLRLLGHRLAHLFNANTRSGARRNIEAHYDLGNRFYRLWLDPTMSYSAALFA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCEEEEE
EGDDQPDADRLAEAQRRKYRRVLDQLGARAGQTVLEIGCGWGGFAEVAVQEYGCRVVGLT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCEEEEEE
LSPAQLGYAQARAAAGGYATEAEFRLCDYRDVEGRFDHIASIEMIEAVGEAYWPTYFRQI
ECHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLLASGGRCVVQAITIDDALFARYRRGTDFIQRYIFPGGMLPAPAQIRRHSARAGLAIT
HHHHHCCCEEEEEEEEHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEE
DDFGFGDHYARTLACWRQRFDAQGEALARLGFPARFTRMWRFYLAYCEAGFRSGDLDVRH
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEE
YTFSRRG
EECCCCC
>Mature Secondary Structure
MNAAESQLPRPGVLERPPAGAAGRTGQDAALPRGARLALDMLDRIEGGSVALTLPDGGTY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCE
CLGRGPQRAHLRVRDLGMFDAALAHGDIGFGEGYMEGDWDCADGDELAALLGLLATNRKV
ECCCCCCHHEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHH
LDTAIRGRFLRLLGHRLAHLFNANTRSGARRNIEAHYDLGNRFYRLWLDPTMSYSAALFA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCEEEEE
EGDDQPDADRLAEAQRRKYRRVLDQLGARAGQTVLEIGCGWGGFAEVAVQEYGCRVVGLT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHCCCEEEEEE
LSPAQLGYAQARAAAGGYATEAEFRLCDYRDVEGRFDHIASIEMIEAVGEAYWPTYFRQI
ECHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLLASGGRCVVQAITIDDALFARYRRGTDFIQRYIFPGGMLPAPAQIRRHSARAGLAIT
HHHHHCCCEEEEEEEEHHHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCEEEE
DDFGFGDHYARTLACWRQRFDAQGEALARLGFPARFTRMWRFYLAYCEAGFRSGDLDVRH
CCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEE
YTFSRRG
EECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]