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The map label for this gene is wza [H]

Identifier: 119899572

GI number: 119899572

Start: 3622352

End: 3622981

Strand: Reverse

Name: wza [H]

Synonym: azo3283

Alternate gene names: 119899572

Gene position: 3622981-3622352 (Counterclockwise)

Preceding gene: 119899574

Following gene: 119899571

Centisome position: 82.79

GC content: 62.86

Gene sequence:

>630_bases
ATGAGCGATAAAGCATCGACGATTTGGGCCGGAGTCCGCGCGTTGGGTTTGGTGTTTGGCGTGCTCGGCGGGCTCGCCGG
CTGTGCTTCCTCTTATCCTCCAGCCCCCATGTCGGCGGCGGATTCCGACTACAACTACGTCATCGGGCCGGGTGACAGCG
TCAACATCGTGGTGTGGCGCAACCCCGAGCTGTCGATGAATGTTCCGGTGCGCCCGGACGGCAAGATCACCACGCCGCTG
GTCGAGGACCTGCCCGCGCTGGGCAAGGATGCCTCCACGCTGGCCCGGGATATCGAGAAGGAACTGTCCAAGTTCATCCG
CGAACCGGTGGTCACTGTCATCGTGTCGCAATTCGTCGGCCCGTACAGTGAGCAGATCCGGGTCGTGGGCGAGGCGGGCA
AGCCGCAGGTGCTGGCCTACAAGCAGAAGATGACCCTGCTCGACGTGATGATCGCCGTGGGCGGCATGACCGAGTTTGCC
GATGGCAATGGTGCCACCATCCTGCGCACGGCCGAAGGCAACAAGCAGTACAGCGTGCGCATCAAGGATCTCGTCAAGCG
CGGCGACGTTTCGGCCAACGTCGAGATGCGTCCGGGCGACGTCCTCATCATTCCGCAGAGCTGGTTCTAA

Upstream 100 bases:

>100_bases
GTGTATTTGTTCCTTCGGCTCTCGCCGATATCTTCGTAGCATGAAAAAATGCGCCGAGGCGTATCATTGCCGTCTTCCAG
AAACAGGGGTGTTCTCGATC

Downstream 100 bases:

>100_bases
GCCGCGTCCCTGGGAGTTTTTACCTTTGCGCCACCAGCGCCAGCGGGTGGGGTGGCGCCGTGTCACCAGGACTGAATCAT
GGAAGAACTAGTAGGGCAGT

Product: polysaccharide export protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MSDKASTIWAGVRALGLVFGVLGGLAGCASSYPPAPMSAADSDYNYVIGPGDSVNIVVWRNPELSMNVPVRPDGKITTPL
VEDLPALGKDASTLARDIEKELSKFIREPVVTVIVSQFVGPYSEQIRVVGEAGKPQVLAYKQKMTLLDVMIAVGGMTEFA
DGNGATILRTAEGNKQYSVRIKDLVKRGDVSANVEMRPGDVLIIPQSWF

Sequences:

>Translated_209_residues
MSDKASTIWAGVRALGLVFGVLGGLAGCASSYPPAPMSAADSDYNYVIGPGDSVNIVVWRNPELSMNVPVRPDGKITTPL
VEDLPALGKDASTLARDIEKELSKFIREPVVTVIVSQFVGPYSEQIRVVGEAGKPQVLAYKQKMTLLDVMIAVGGMTEFA
DGNGATILRTAEGNKQYSVRIKDLVKRGDVSANVEMRPGDVLIIPQSWF
>Mature_208_residues
SDKASTIWAGVRALGLVFGVLGGLAGCASSYPPAPMSAADSDYNYVIGPGDSVNIVVWRNPELSMNVPVRPDGKITTPLV
EDLPALGKDASTLARDIEKELSKFIREPVVTVIVSQFVGPYSEQIRVVGEAGKPQVLAYKQKMTLLDVMIAVGGMTEFAD
GNGATILRTAEGNKQYSVRIKDLVKRGDVSANVEMRPGDVLIIPQSWF

Specific function: Probably involved in the export of the extracellular polysaccharide colanic acid from the cell to medium [H]

COG id: COG1596

COG function: function code M; Periplasmic protein involved in polysaccharide export

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BexD/CtrA/VexA family [H]

Homologues:

Organism=Escherichia coli, GI1788376, Length=187, Percent_Identity=26.7379679144385, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1787218, Length=178, Percent_Identity=31.4606741573034, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003715
- InterPro:   IPR019554 [H]

Pfam domain/function: PF02563 Poly_export; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 22396; Mature: 22265

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKASTIWAGVRALGLVFGVLGGLAGCASSYPPAPMSAADSDYNYVIGPGDSVNIVVWR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEEEEE
NPELSMNVPVRPDGKITTPLVEDLPALGKDASTLARDIEKELSKFIREPVVTVIVSQFVG
CCCCEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PYSEQIRVVGEAGKPQVLAYKQKMTLLDVMIAVGGMTEFADGNGATILRTAEGNKQYSVR
CCCHHEEEEECCCCCCEEEHHHHHHHHHHHHHHCCCCEECCCCCCEEEEECCCCCEEEEE
IKDLVKRGDVSANVEMRPGDVLIIPQSWF
HHHHHHHCCCCCEEEECCCCEEEECCCCC
>Mature Secondary Structure 
SDKASTIWAGVRALGLVFGVLGGLAGCASSYPPAPMSAADSDYNYVIGPGDSVNIVVWR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEEEEE
NPELSMNVPVRPDGKITTPLVEDLPALGKDASTLARDIEKELSKFIREPVVTVIVSQFVG
CCCCEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PYSEQIRVVGEAGKPQVLAYKQKMTLLDVMIAVGGMTEFADGNGATILRTAEGNKQYSVR
CCCHHEEEEECCCCCCEEEHHHHHHHHHHHHHHCCCCEECCCCCCEEEEECCCCCEEEEE
IKDLVKRGDVSANVEMRPGDVLIIPQSWF
HHHHHHHCCCCCEEEECCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]