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The map label for this gene is asnB1 [H]

Identifier: 119899560

GI number: 119899560

Start: 3606175

End: 3608103

Strand: Reverse

Name: asnB1 [H]

Synonym: azo3271

Alternate gene names: 119899560

Gene position: 3608103-3606175 (Counterclockwise)

Preceding gene: 119899561

Following gene: 119899559

Centisome position: 82.45

GC content: 68.27

Gene sequence:

>1929_bases
ATGTGCGGCATCCACGGTTTCTATCGCTTCGATGGCCGCGCGGTCGAGCGCGCGCACCTGTCCGCAATGGGGGATGTCAC
CCGACACCGCGGGCCCGATGACGAAGGCCAGCACATCGAGCCGGGCGGACGCTGCGGCATCGCGATGCGTCGCCTGTCCA
TCATCGACCTGGCCGGCGGCCATCAGCCGATCTCCAATGCCGACGACACCTTGTGGGTGGTCTGCAACGGCGAGATCTAC
AATTTCCGCGAGTTGCGCCAGGAGCTGCAGGCGCGCGGTTACCGCTTCAAGACGGGGTCCGACAGCGAGGTCCTGCTGCA
CCTCTACGACCTGCACGGAGACGATTTCGTCCATCGACTGGACGGCATGTTCAATTTCGCGCTGTGGGACGCCCGTCGCC
AGCGCCTGCTGATCGGCCGCGACCACCTCGGCGTGAAGCCGCTGTACGTGCACCAGTCCGCGAGCCTGCTTGCCTTCGCC
ACCGAGGCCAAGGCGCTGCTGGCGCTGCCCGGCATGCAGCCCGCGCTCAACCGCGAGGTGCTGGGCGACTACCTCCATCT
CGGCTACGTGCCCGCGCCGCATTCGATGTTCGCCGGGATCCGCAAGCTGCCGCCGGCCACGCTGCTGTCGGTGGATGCCC
AGGGCGTGCGCGAGTGGCGCTACTGGCGTCTGCCGCAAACCGTGGACCGCACGGTGGGCGAGGCCGAATGGGTCGAGCGC
ATCCGCGCCGGCATGGAGGCTGCGGTGCAACGGCAGATGGTGGCGGACGTCCCGATCGGCGCCTTCCTCTCCGGCGGAGT
CGATTCCAGCGCGGTGGTCGCCTACATGGCGCGCCATTCCGACCAGCCGATTCGCACCTATGCCATCGGCTTTGAAGGCG
GCGAGGCCGAGCAGCTGTACAACGAACTGCCCTACGCGCGCCAGGTGGCGGGGCTGTTCGGCACCGAGCATCGCGAGATC
GTCGTCCGTCCGGATGTCGTCGGCCTGCTGCCGCGGCTGCTGTGGCACATGGACGAGCCGGTGGCCGACTCCGCTTTCAT
CACCACTTACCTGGTGTCCGAGTTCGCCCGTCGCGACGTCAAGGTCATCCTGTCGGGGGTCGGCGGGGACGAACTGTTCG
GCGGGTATCGCCGTTATCTCGGCGGCCACTACCTCGACAAGCTCGCCCGCCTGCCACTGTGGTCGCGCCGACTGATGGCA
CGGACCGCCGGGCTGCTGCCGAGCGATCGCCACTCCAAGGTGCTCAACACCCTGCGGCTCGCGCGCGGCCTCCTGGCTTC
TTCCGAACTGGCGCCCGACGACCGCTACCGCCACTACCTGCAGGTGCTGCCGCGCGACGACGTGGCTGCGCTGACGCGCG
GGCAGCCGGGTGGCAGCGATGCGCTTGCCGCTGCCTTTGCCGCGGCTGGCAACGACGATGCGCTCAACCGCATGTTCGCG
GTGGACGCCGAAACCCAGTTGCCGGACGACCTGCTGCTGCTTACCGACAAGATGAGCATGGCGGTGTCGCTCGAATGCCG
CGTGCCGCTGCTCGACCGCGCCCTGGTCGAACTCGCCGCGTCCATTCCGGAAAGCCTCAAGGTCCGCAACGGACGCCTCA
AGCACCTGATGAAGTCGGCGCTCGCCGACGTGTTGCCGCCCGACATCCTCGACCGCCGCAAGCGCGGTTTCGGTACGCCG
ATGGGCGCGTGGCTGAAGCAGCAACTGGCGCCGGTGCTGCGCCGCCTGCTCGCCGCCGATGTCGTGCGCGACCGCGGCCT
GTTCGACCCCGCGGTCGTCGCCCGCCTGGTGGCCGACCACGAGGCCGCGCGGATCGACGGTACCGATGCCTTGCTCGCGC
TGATGAATCTTGAAATCTGGAGCCGCGTGCATCTCGACCGCCACAGCGTCGACGATGTCACCGCGGAGTTGAAGAGCCTG
ATCGCATGA

Upstream 100 bases:

>100_bases
GAGCGCACCTGGAAGAACAGTGTCGCCCGCTATCGCGAGGTCTACCGGCGCGCGCTGGCCAGCCGCGGGCGCGACATGAC
GCTGGCGGAGGCACGCTGAG

Downstream 100 bases:

>100_bases
AGATCCTCTACGTCTGCCATCGCTTTCCCTTCCCGCCCAAGCGGGGCGGAAAGATCCGGCCGTTCAACATGATCCGCCAC
CTGTCGCAGCAGCATCAGGT

Product: asparagine synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 642; Mature: 642

Protein sequence:

>642_residues
MCGIHGFYRFDGRAVERAHLSAMGDVTRHRGPDDEGQHIEPGGRCGIAMRRLSIIDLAGGHQPISNADDTLWVVCNGEIY
NFRELRQELQARGYRFKTGSDSEVLLHLYDLHGDDFVHRLDGMFNFALWDARRQRLLIGRDHLGVKPLYVHQSASLLAFA
TEAKALLALPGMQPALNREVLGDYLHLGYVPAPHSMFAGIRKLPPATLLSVDAQGVREWRYWRLPQTVDRTVGEAEWVER
IRAGMEAAVQRQMVADVPIGAFLSGGVDSSAVVAYMARHSDQPIRTYAIGFEGGEAEQLYNELPYARQVAGLFGTEHREI
VVRPDVVGLLPRLLWHMDEPVADSAFITTYLVSEFARRDVKVILSGVGGDELFGGYRRYLGGHYLDKLARLPLWSRRLMA
RTAGLLPSDRHSKVLNTLRLARGLLASSELAPDDRYRHYLQVLPRDDVAALTRGQPGGSDALAAAFAAAGNDDALNRMFA
VDAETQLPDDLLLLTDKMSMAVSLECRVPLLDRALVELAASIPESLKVRNGRLKHLMKSALADVLPPDILDRRKRGFGTP
MGAWLKQQLAPVLRRLLAADVVRDRGLFDPAVVARLVADHEAARIDGTDALLALMNLEIWSRVHLDRHSVDDVTAELKSL
IA

Sequences:

>Translated_642_residues
MCGIHGFYRFDGRAVERAHLSAMGDVTRHRGPDDEGQHIEPGGRCGIAMRRLSIIDLAGGHQPISNADDTLWVVCNGEIY
NFRELRQELQARGYRFKTGSDSEVLLHLYDLHGDDFVHRLDGMFNFALWDARRQRLLIGRDHLGVKPLYVHQSASLLAFA
TEAKALLALPGMQPALNREVLGDYLHLGYVPAPHSMFAGIRKLPPATLLSVDAQGVREWRYWRLPQTVDRTVGEAEWVER
IRAGMEAAVQRQMVADVPIGAFLSGGVDSSAVVAYMARHSDQPIRTYAIGFEGGEAEQLYNELPYARQVAGLFGTEHREI
VVRPDVVGLLPRLLWHMDEPVADSAFITTYLVSEFARRDVKVILSGVGGDELFGGYRRYLGGHYLDKLARLPLWSRRLMA
RTAGLLPSDRHSKVLNTLRLARGLLASSELAPDDRYRHYLQVLPRDDVAALTRGQPGGSDALAAAFAAAGNDDALNRMFA
VDAETQLPDDLLLLTDKMSMAVSLECRVPLLDRALVELAASIPESLKVRNGRLKHLMKSALADVLPPDILDRRKRGFGTP
MGAWLKQQLAPVLRRLLAADVVRDRGLFDPAVVARLVADHEAARIDGTDALLALMNLEIWSRVHLDRHSVDDVTAELKSL
IA
>Mature_642_residues
MCGIHGFYRFDGRAVERAHLSAMGDVTRHRGPDDEGQHIEPGGRCGIAMRRLSIIDLAGGHQPISNADDTLWVVCNGEIY
NFRELRQELQARGYRFKTGSDSEVLLHLYDLHGDDFVHRLDGMFNFALWDARRQRLLIGRDHLGVKPLYVHQSASLLAFA
TEAKALLALPGMQPALNREVLGDYLHLGYVPAPHSMFAGIRKLPPATLLSVDAQGVREWRYWRLPQTVDRTVGEAEWVER
IRAGMEAAVQRQMVADVPIGAFLSGGVDSSAVVAYMARHSDQPIRTYAIGFEGGEAEQLYNELPYARQVAGLFGTEHREI
VVRPDVVGLLPRLLWHMDEPVADSAFITTYLVSEFARRDVKVILSGVGGDELFGGYRRYLGGHYLDKLARLPLWSRRLMA
RTAGLLPSDRHSKVLNTLRLARGLLASSELAPDDRYRHYLQVLPRDDVAALTRGQPGGSDALAAAFAAAGNDDALNRMFA
VDAETQLPDDLLLLTDKMSMAVSLECRVPLLDRALVELAASIPESLKVRNGRLKHLMKSALADVLPPDILDRRKRGFGTP
MGAWLKQQLAPVLRRLLAADVVRDRGLFDPAVVARLVADHEAARIDGTDALLALMNLEIWSRVHLDRHSVDDVTAELKSL
IA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=394, Percent_Identity=30.4568527918782, Blast_Score=143, Evalue=5e-34,
Organism=Homo sapiens, GI168229252, Length=394, Percent_Identity=30.4568527918782, Blast_Score=143, Evalue=5e-34,
Organism=Homo sapiens, GI168229250, Length=394, Percent_Identity=30.4568527918782, Blast_Score=143, Evalue=5e-34,
Organism=Homo sapiens, GI296010848, Length=366, Percent_Identity=30.327868852459, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI1786889, Length=397, Percent_Identity=33.5012594458438, Blast_Score=167, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI71993933, Length=389, Percent_Identity=29.5629820051414, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25147557, Length=389, Percent_Identity=29.5629820051414, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560178, Length=390, Percent_Identity=28.4615384615385, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25147560, Length=355, Percent_Identity=28.169014084507, Blast_Score=100, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6321563, Length=416, Percent_Identity=28.125, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6325403, Length=417, Percent_Identity=27.0983213429257, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI45553209, Length=469, Percent_Identity=27.0788912579957, Blast_Score=145, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 71249; Mature: 71249

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIHGFYRFDGRAVERAHLSAMGDVTRHRGPDDEGQHIEPGGRCGIAMRRLSIIDLAGG
CCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHEEECCC
HQPISNADDTLWVVCNGEIYNFRELRQELQARGYRFKTGSDSEVLLHLYDLHGDDFVHRL
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCEEECCCCCCEEEEEEECCCCCHHHHHH
DGMFNFALWDARRQRLLIGRDHLGVKPLYVHQSASLLAFATEAKALLALPGMQPALNREV
HHHHHHHHHHHHHHHEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHH
LGDYLHLGYVPAPHSMFAGIRKLPPATLLSVDAQGVREWRYWRLPQTVDRTVGEAEWVER
HHHHHHHCCCCCCHHHHHHHHHCCCHHEEECCHHHHHHHHHCCCCHHHHHHCCHHHHHHH
IRAGMEAAVQRQMVADVPIGAFLSGGVDSSAVVAYMARHSDQPIRTYAIGFEGGEAEQLY
HHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
NELPYARQVAGLFGTEHREIVVRPDVVGLLPRLLWHMDEPVADSAFITTYLVSEFARRDV
HHCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCH
KVILSGVGGDELFGGYRRYLGGHYLDKLARLPLWSRRLMARTAGLLPSDRHSKVLNTLRL
HHHHCCCCCHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ARGLLASSELAPDDRYRHYLQVLPRDDVAALTRGQPGGSDALAAAFAAAGNDDALNRMFA
HHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHEEE
VDAETQLPDDLLLLTDKMSMAVSLECRVPLLDRALVELAASIPESLKVRNGRLKHLMKSA
ECCCCCCCCCCEEEECCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHH
LADVLPPDILDRRKRGFGTPMGAWLKQQLAPVLRRLLAADVVRDRGLFDPAVVARLVADH
HHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
EAARIDGTDALLALMNLEIWSRVHLDRHSVDDVTAELKSLIA
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MCGIHGFYRFDGRAVERAHLSAMGDVTRHRGPDDEGQHIEPGGRCGIAMRRLSIIDLAGG
CCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHEEECCC
HQPISNADDTLWVVCNGEIYNFRELRQELQARGYRFKTGSDSEVLLHLYDLHGDDFVHRL
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCEEECCCCCCEEEEEEECCCCCHHHHHH
DGMFNFALWDARRQRLLIGRDHLGVKPLYVHQSASLLAFATEAKALLALPGMQPALNREV
HHHHHHHHHHHHHHHEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHH
LGDYLHLGYVPAPHSMFAGIRKLPPATLLSVDAQGVREWRYWRLPQTVDRTVGEAEWVER
HHHHHHHCCCCCCHHHHHHHHHCCCHHEEECCHHHHHHHHHCCCCHHHHHHCCHHHHHHH
IRAGMEAAVQRQMVADVPIGAFLSGGVDSSAVVAYMARHSDQPIRTYAIGFEGGEAEQLY
HHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHH
NELPYARQVAGLFGTEHREIVVRPDVVGLLPRLLWHMDEPVADSAFITTYLVSEFARRDV
HHCCHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCH
KVILSGVGGDELFGGYRRYLGGHYLDKLARLPLWSRRLMARTAGLLPSDRHSKVLNTLRL
HHHHCCCCCHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
ARGLLASSELAPDDRYRHYLQVLPRDDVAALTRGQPGGSDALAAAFAAAGNDDALNRMFA
HHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHEEE
VDAETQLPDDLLLLTDKMSMAVSLECRVPLLDRALVELAASIPESLKVRNGRLKHLMKSA
ECCCCCCCCCCEEEECCCCEEEEEEECCCHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHH
LADVLPPDILDRRKRGFGTPMGAWLKQQLAPVLRRLLAADVVRDRGLFDPAVVARLVADH
HHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
EAARIDGTDALLALMNLEIWSRVHLDRHSVDDVTAELKSLIA
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]