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The map label for this gene is prpE [H]

Identifier: 119899469

GI number: 119899469

Start: 3489012

End: 3490904

Strand: Reverse

Name: prpE [H]

Synonym: azo3179

Alternate gene names: 119899469

Gene position: 3490904-3489012 (Counterclockwise)

Preceding gene: 119899470

Following gene: 119899465

Centisome position: 79.77

GC content: 64.55

Gene sequence:

>1893_bases
ATGAGCAACTACAAGGAGTTCCACCGTAGGTCCATCGACCAGCGCGACGAGTTCTGGGCCGAGCAGGCCCAACTTGTGGA
CTGGCACAAGCCGGCCGAGCAGATCTGCGACTTTTCGAAGCCGCCGTTCGTCAAGTGGTTCAAGGGCGGCGAAACCAACC
TCTGCTACAACGCGGTGGACCGTCACGCGGCGAAACGTCCCGACGACCGCGCCCTGGTCTACATCTCCACCGAAACCAAC
GAGGAGAAGGTGTATTCCTTCGCCGAGTTGCAGCGCGAAGTCGAGCGCATGGCGGCGATCTACCAGGATCTCGGGGTCAA
GCGCGGCGACCGTGTGCTGATCTACATGCCGATGATCGCCGAGGCCGCTTTTGCGATGCTGGCCTGCGCGCGCATCGGCG
CGATCCACTCGGTGGTGTTTGGCGGTTTCGCGGCTGGTTCGCTCGCCACCCGTATCGACGATGCCAAGCCGGTGCTGATG
GTCAGCTCCGACGCCGGCATGCGCAACGGCAAGCCGGTGCCCTACAAGCACCTGGTGGACGAGGCCTGCAAGCTGGCGGA
ATTCCCGCCGGCCAAGGTGTTGATCGTCGACCGCGGCCTCGACAAGGGCTTCCCCAAGGTGGAAAGCCGTGACGTCGATT
ACGCCGAACTGCGCGCCAAGCACATCGACGCCAAGGTGCCGGTGACCTGGCTGGAATCCTCCGAGCCGAGCTACATCCTT
TACACCTCCGGCACCACCGGCAAGCCCAAGGGCGTGCAGCGCGACACCGGCGGCTACACCGTGGCGTTGGCGGCCTCGAT
GAAGCACATCTTCACCGGCGGCAGCGGCGAAACCATGTTCTCCACCTCGGACATCGGCTGGGTGGTGGGCCACAGCTACA
TCATCTACGGCCCGCTGATCGCCGGCATGGCCACCATCATGTACGAAGGCACGCCGCTGCGTCCGGATGCCGGCATCTGG
TGGCAGATCGTCGAGAAGTACAAGGTCAGCGTGATGTTCTCGGCGCCGACCGCGGTGCGCGTGCTGAAGAAGCAGGACCC
GGCCTTCCTCAAGAAGTACGACCTGTCCTCGCTCAAGCACCTGTTCCTGGCCGGCGAACCGCTGGACGAGACCTCGCACA
AGTGGATCATGAGCGAACTCGGCATTCCGGTGATCGACAACTACTGGCAGACCGAGACCGGCTGGCCGATGCTCGCGATC
GTGCGCGGCGTGGAAGACAGCAAGATCAAGCTCGGTTCGCCCGCCTTCCCGGTGTATGGCTACGACCTCCGCATCTTCCG
TGAGGACGGCAGCGAATGCGGTCCGAACGAGAAGGGTATCGTCGGCATCGTGCCGCCGCTGCCGCCGGGCTGCCTGTCCA
CCGTGTGGGGTCAGGACGAGCGCTTCGTCTCCACCTACTTCAGCCTGTTCACCGATCCGGTGGTGTATTCGTCGTCCGAC
TGGGGCATCAAGGACGAAAACGGCTACCACACCATCCTCGGCCGCATGGACGACGTCATCAACGTCGCCGGACACCGTCT
CGGCACCCGCGAGATCGAGGAAGCGGTGCAGACCCACCCGGCGATCGCCGAAGTGGCGGTGGTCGGCGTGCACGACGAGC
TGAAGGGCCAGATGCCGATGGCTTTCGCGGTGGTGAAGGACGCGAGCACCGTCGACACCGCCGAGAAGCGCGCCGCGCTC
GAGAAGGAAGTGATGAAGAAGGTGGACGAAAGCCTTGGCGCGATCGCGCGCCCGGCCCGCGTGCACTTCATCAACGGCCT
GCCGAAGACGCGTTCGGGCAAGATGCTGCGCCGCTCGATCCAGGCGCTGGCCGAAGGCCGTGATGCCGGCGACCTGACCA
CGATCGACGATCCGACCACGCTGGATCAGATCAAGGCCGCCTTGGCGGGCTGA

Upstream 100 bases:

>100_bases
AATAGCAGCAATAGCAAACGTGGCGGGCGGCCGCGCTGGGGAGCGCCGGGCGCCGTTGCGTCAATCAAAAATGTAGGGAA
TCTGGAAGGGAGAGGATTTG

Downstream 100 bases:

>100_bases
GGTCAGACAGCAAAGAAAAAGCCCGCGCAAGCGGGCTTTTTTTATTGTGTGCCCATGGGGCAGGGCTAGCGCGCCGGGCC
CGACAGGGAGCGGCCACCTG

Product: propionyl-CoA synthetase

Products: NA

Alternate protein names: Propionyl-CoA synthetase [H]

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MSNYKEFHRRSIDQRDEFWAEQAQLVDWHKPAEQICDFSKPPFVKWFKGGETNLCYNAVDRHAAKRPDDRALVYISTETN
EEKVYSFAELQREVERMAAIYQDLGVKRGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFAAGSLATRIDDAKPVLM
VSSDAGMRNGKPVPYKHLVDEACKLAEFPPAKVLIVDRGLDKGFPKVESRDVDYAELRAKHIDAKVPVTWLESSEPSYIL
YTSGTTGKPKGVQRDTGGYTVALAASMKHIFTGGSGETMFSTSDIGWVVGHSYIIYGPLIAGMATIMYEGTPLRPDAGIW
WQIVEKYKVSVMFSAPTAVRVLKKQDPAFLKKYDLSSLKHLFLAGEPLDETSHKWIMSELGIPVIDNYWQTETGWPMLAI
VRGVEDSKIKLGSPAFPVYGYDLRIFREDGSECGPNEKGIVGIVPPLPPGCLSTVWGQDERFVSTYFSLFTDPVVYSSSD
WGIKDENGYHTILGRMDDVINVAGHRLGTREIEEAVQTHPAIAEVAVVGVHDELKGQMPMAFAVVKDASTVDTAEKRAAL
EKEVMKKVDESLGAIARPARVHFINGLPKTRSGKMLRRSIQALAEGRDAGDLTTIDDPTTLDQIKAALAG

Sequences:

>Translated_630_residues
MSNYKEFHRRSIDQRDEFWAEQAQLVDWHKPAEQICDFSKPPFVKWFKGGETNLCYNAVDRHAAKRPDDRALVYISTETN
EEKVYSFAELQREVERMAAIYQDLGVKRGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFAAGSLATRIDDAKPVLM
VSSDAGMRNGKPVPYKHLVDEACKLAEFPPAKVLIVDRGLDKGFPKVESRDVDYAELRAKHIDAKVPVTWLESSEPSYIL
YTSGTTGKPKGVQRDTGGYTVALAASMKHIFTGGSGETMFSTSDIGWVVGHSYIIYGPLIAGMATIMYEGTPLRPDAGIW
WQIVEKYKVSVMFSAPTAVRVLKKQDPAFLKKYDLSSLKHLFLAGEPLDETSHKWIMSELGIPVIDNYWQTETGWPMLAI
VRGVEDSKIKLGSPAFPVYGYDLRIFREDGSECGPNEKGIVGIVPPLPPGCLSTVWGQDERFVSTYFSLFTDPVVYSSSD
WGIKDENGYHTILGRMDDVINVAGHRLGTREIEEAVQTHPAIAEVAVVGVHDELKGQMPMAFAVVKDASTVDTAEKRAAL
EKEVMKKVDESLGAIARPARVHFINGLPKTRSGKMLRRSIQALAEGRDAGDLTTIDDPTTLDQIKAALAG
>Mature_629_residues
SNYKEFHRRSIDQRDEFWAEQAQLVDWHKPAEQICDFSKPPFVKWFKGGETNLCYNAVDRHAAKRPDDRALVYISTETNE
EKVYSFAELQREVERMAAIYQDLGVKRGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFAAGSLATRIDDAKPVLMV
SSDAGMRNGKPVPYKHLVDEACKLAEFPPAKVLIVDRGLDKGFPKVESRDVDYAELRAKHIDAKVPVTWLESSEPSYILY
TSGTTGKPKGVQRDTGGYTVALAASMKHIFTGGSGETMFSTSDIGWVVGHSYIIYGPLIAGMATIMYEGTPLRPDAGIWW
QIVEKYKVSVMFSAPTAVRVLKKQDPAFLKKYDLSSLKHLFLAGEPLDETSHKWIMSELGIPVIDNYWQTETGWPMLAIV
RGVEDSKIKLGSPAFPVYGYDLRIFREDGSECGPNEKGIVGIVPPLPPGCLSTVWGQDERFVSTYFSLFTDPVVYSSSDW
GIKDENGYHTILGRMDDVINVAGHRLGTREIEEAVQTHPAIAEVAVVGVHDELKGQMPMAFAVVKDASTVDTAEKRAALE
KEVMKKVDESLGAIARPARVHFINGLPKTRSGKMLRRSIQALAEGRDAGDLTTIDDPTTLDQIKAALAG

Specific function: Catalyzes the synthesis of propionyl-CoA from propionate and CoA. Also converts acetate to acetyl-CoA but with a lower specific activity [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=633, Percent_Identity=40.6003159557662, Blast_Score=479, Evalue=1e-135,
Organism=Homo sapiens, GI8923896, Length=660, Percent_Identity=37.1212121212121, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI253970489, Length=673, Percent_Identity=36.4041604754829, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI28416953, Length=633, Percent_Identity=37.4407582938389, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI42544132, Length=544, Percent_Identity=28.4926470588235, Blast_Score=193, Evalue=4e-49,
Organism=Homo sapiens, GI38505220, Length=566, Percent_Identity=26.1484098939929, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI31982927, Length=661, Percent_Identity=25.5673222390318, Blast_Score=177, Evalue=3e-44,
Organism=Homo sapiens, GI58082049, Length=551, Percent_Identity=27.7676950998185, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI122937307, Length=558, Percent_Identity=26.8817204301075, Blast_Score=170, Evalue=5e-42,
Organism=Homo sapiens, GI115511026, Length=569, Percent_Identity=27.0650263620387, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI157311624, Length=551, Percent_Identity=28.1306715063521, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI157311622, Length=551, Percent_Identity=28.1306715063521, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI42544134, Length=365, Percent_Identity=26.3013698630137, Blast_Score=113, Evalue=5e-25,
Organism=Homo sapiens, GI195947378, Length=449, Percent_Identity=22.71714922049, Blast_Score=87, Evalue=7e-17,
Organism=Escherichia coli, GI1786529, Length=627, Percent_Identity=60.6060606060606, Blast_Score=786, Evalue=0.0,
Organism=Escherichia coli, GI1790505, Length=630, Percent_Identity=38.7301587301587, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI221142682, Length=536, Percent_Identity=21.2686567164179, Blast_Score=106, Evalue=6e-24,
Organism=Escherichia coli, GI145693145, Length=554, Percent_Identity=23.4657039711191, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1788107, Length=552, Percent_Identity=23.0072463768116, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786810, Length=551, Percent_Identity=22.1415607985481, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1786801, Length=488, Percent_Identity=22.3360655737705, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71982997, Length=649, Percent_Identity=35.7473035439137, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71983001, Length=649, Percent_Identity=35.7473035439137, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17569507, Length=644, Percent_Identity=23.2919254658385, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17531443, Length=563, Percent_Identity=25.3996447602131, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=561, Percent_Identity=24.777183600713, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=578, Percent_Identity=23.356401384083, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71996755, Length=534, Percent_Identity=20.9737827715356, Blast_Score=96, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17538037, Length=573, Percent_Identity=23.7347294938918, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17560140, Length=523, Percent_Identity=21.9885277246654, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6319264, Length=637, Percent_Identity=36.734693877551, Blast_Score=427, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6323182, Length=644, Percent_Identity=37.2670807453416, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6319699, Length=380, Percent_Identity=23.6842105263158, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21355225, Length=641, Percent_Identity=40.2496099843994, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24667955, Length=654, Percent_Identity=36.085626911315, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI62472339, Length=558, Percent_Identity=36.5591397849462, Blast_Score=355, Evalue=4e-98,
Organism=Drosophila melanogaster, GI24667959, Length=528, Percent_Identity=36.1742424242424, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI21355181, Length=533, Percent_Identity=25.703564727955, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21356947, Length=381, Percent_Identity=24.1469816272966, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24656500, Length=375, Percent_Identity=23.7333333333333, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648253, Length=326, Percent_Identity=25.1533742331288, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24648255, Length=326, Percent_Identity=25.4601226993865, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR012694 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.17 [H]

Molecular weight: Translated: 69663; Mature: 69532

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNYKEFHRRSIDQRDEFWAEQAQLVDWHKPAEQICDFSKPPFVKWFKGGETNLCYNAVD
CCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEECCCCCCEEHHHHH
RHAAKRPDDRALVYISTETNEEKVYSFAELQREVERMAAIYQDLGVKRGDRVLIYMPMIA
HHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHH
EAAFAMLACARIGAIHSVVFGGFAAGSLATRIDDAKPVLMVSSDAGMRNGKPVPYKHLVD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHH
EACKLAEFPPAKVLIVDRGLDKGFPKVESRDVDYAELRAKHIDAKVPVTWLESSEPSYIL
HHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCEEE
YTSGTTGKPKGVQRDTGGYTVALAASMKHIFTGGSGETMFSTSDIGWVVGHSYIIYGPLI
EECCCCCCCCCCCCCCCCEEEEEEEHHHHHEECCCCCEEEECCCCCHHHCCCEEEEHHHH
AGMATIMYEGTPLRPDAGIWWQIVEKYKVSVMFSAPTAVRVLKKQDPAFLKKYDLSSLKH
HHHHHHHCCCCCCCCCCCHHHHHHHHHEEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHH
LFLAGEPLDETSHKWIMSELGIPVIDNYWQTETGWPMLAIVRGVEDSKIKLGSPAFPVYG
HHHCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCHHHEEECCCCCEEEECCCCCCEEC
YDLRIFREDGSECGPNEKGIVGIVPPLPPGCLSTVWGQDERFVSTYFSLFTDPVVYSSSD
CEEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEECCCC
WGIKDENGYHTILGRMDDVINVAGHRLGTREIEEAVQTHPAIAEVAVVGVHDELKGQMPM
CCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHEEEEECCHHHCCCCCE
AFAVVKDASTVDTAEKRAALEKEVMKKVDESLGAIARPARVHFINGLPKTRSGKMLRRSI
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
QALAEGRDAGDLTTIDDPTTLDQIKAALAG
HHHHCCCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SNYKEFHRRSIDQRDEFWAEQAQLVDWHKPAEQICDFSKPPFVKWFKGGETNLCYNAVD
CCHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEECCCCCCEEHHHHH
RHAAKRPDDRALVYISTETNEEKVYSFAELQREVERMAAIYQDLGVKRGDRVLIYMPMIA
HHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHH
EAAFAMLACARIGAIHSVVFGGFAAGSLATRIDDAKPVLMVSSDAGMRNGKPVPYKHLVD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHHH
EACKLAEFPPAKVLIVDRGLDKGFPKVESRDVDYAELRAKHIDAKVPVTWLESSEPSYIL
HHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCEEE
YTSGTTGKPKGVQRDTGGYTVALAASMKHIFTGGSGETMFSTSDIGWVVGHSYIIYGPLI
EECCCCCCCCCCCCCCCCEEEEEEEHHHHHEECCCCCEEEECCCCCHHHCCCEEEEHHHH
AGMATIMYEGTPLRPDAGIWWQIVEKYKVSVMFSAPTAVRVLKKQDPAFLKKYDLSSLKH
HHHHHHHCCCCCCCCCCCHHHHHHHHHEEEEEEECCHHHHHHHCCCCHHHHHHHHHHHHH
LFLAGEPLDETSHKWIMSELGIPVIDNYWQTETGWPMLAIVRGVEDSKIKLGSPAFPVYG
HHHCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCHHHEEECCCCCEEEECCCCCCEEC
YDLRIFREDGSECGPNEKGIVGIVPPLPPGCLSTVWGQDERFVSTYFSLFTDPVVYSSSD
CEEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHCCCEEECCCC
WGIKDENGYHTILGRMDDVINVAGHRLGTREIEEAVQTHPAIAEVAVVGVHDELKGQMPM
CCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHEEEEECCHHHCCCCCE
AFAVVKDASTVDTAEKRAALEKEVMKKVDESLGAIARPARVHFINGLPKTRSGKMLRRSI
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
QALAEGRDAGDLTTIDDPTTLDQIKAALAG
HHHHCCCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]