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The map label for this gene is ilvI [H]

Identifier: 119899444

GI number: 119899444

Start: 3462564

End: 3464276

Strand: Direct

Name: ilvI [H]

Synonym: azo3154

Alternate gene names: 119899444

Gene position: 3462564-3464276 (Clockwise)

Preceding gene: 119899436

Following gene: 119899445

Centisome position: 79.13

GC content: 64.27

Gene sequence:

>1713_bases
ATGGTGCTGACCGGAGCAGAAATTGTAATCAGGTGTCTGCAGGAAGAAAAGGTCGAATACGTGTTCGGCTACCCCGGCGG
CGCCGTGCTCTACATCTACGACGCGCTCTTCCAGCAGGAACAGGTCCGCCACGTTCTCGTCCGCCACGAACAGGCCGCTG
TGCACGCCGCCGACGGCTTCGCGCGCAGCACCGAAAAGGTCGGCGTCGCGCTGGTCACCTCCGGTCCGGGTGCCACCAAT
GCCGTCACCGGCATCGCCACCGCGTTCTGCGACTCGATCCCGATGGTCATCATCTCCGGCCAGGTGCCCACGGCGGCAAT
CGGCCAGGATGCCTTCCAGGAAGTGGACACCGTCGGCATCACCCGCCCCTGTGTGAAGCACAACTTCCTGGTGAAGGACG
TGCGCGACATCGCCGCCACCATCAAGAAGGCCTTCTACCTCGCCAAGACCGGTCGTCCCGGTCCGGTGCTGGTCGACATC
CCCAAGGACATCACCATCGCCAAGTGCGACTTCGAGTATCCGAAGGAAATCTCGATGCGCTCGTACAGCCCGGTGGTCAA
GGGCCACCAGGGCCAGATCAAGAAGGCGGTGCAGCTGCTGCTCGAAGCCAAGCGCCCGATGATCTATACCGGCGGCGGCG
TGGTGCTGGCCAATGCGGCCGAACAACTCACCAAGCTCACCCGCCTGCTCGGGTTCCCGGTCACCAACACGCTGATGGGC
CTGGGCGGCTATCCCGCCTCCGATCGCCAGTACCTCGGCATGCCGGGCATGCACGGCACCTACGAAGCCAACATGGCGAT
GCACTACAGCGACGTGCTGCTCGCCGTCGGCGCCCGCTTCGACGACCGCGTGATCGGCAACCCGGCCAATTTCGCCGAAG
AGCCGCGCAAGATCATCCACATCGACATCGACCCCTCGTCGATCTCCAAGCGCGTCAAGGTGGACGTGCCCATCGTCGGC
GACCTCAAGGACGTGCTCGACGAGATGATCCGCCAGCTCGAAGCTGCGCCCACCCGCCCGGACGCGAGCAATCTCGCCGC
GTGGTGGAAGCAGGTCGAGGAATGGCGCAGCCGCAAGTGCATGGAATACAAGAACTCGGACGAGATCATCAAGCCGCAGT
TCGTGGTCCAGAAGCTGTGGGAAGTGACCGGCGGCGACGCCATCGTCACCTCCGACGTCGGCCAGCACCAGATGTGGGCC
TCGCAGTACTACCGCTTCGACAAGCCGCGCCGCTGGCTCAACTCCGGCGGCCTCGGCACCATGGGCGTCGGCCTGCCCTA
TGCCATGGGCGCCCAGTTCGCCCATCCGGACATGCCGGTTGCCTGCGTCACCGGCGAGGCCTCGATCCAGATGTGCATCC
AGGAACTGTCCACCTGCAAGCAGTTCCGCCTGCCGATCAAGATCTGCAACCTCAACAACCGCTACCTCGGCATGGTCCGG
CAGTGGCAGCAGCTCTTCCACGGCGGTCGCTACTCCGAGTCCTACATGGATTCGCTGCCGGACTTCGCCAAGCTGGCCTC
GGCCTACGGCCACGTCGGCATCCGCGTGGACAAGCCCGCGGACGTGGAAGGCGCGCTGCGCGAAGCCTTCACGACGCACA
AGAACGACCTCGTCTTCCTCGATTTCCAGGTGGATCCGACCGAGAACGTCTATCCGATGGTGCAGGGTGGCAAGGGCCTG
ACCGAGATGATCCTCTCGGCCGAAGACCTGTAA

Upstream 100 bases:

>100_bases
TCATCTGGCGCGCTGCCGCGGCGCGCCGCAGCCTTGACCAATCCCCCCGCGGACGGTAACGTTCGTCGTTTCCTTGAACG
TGATACTGAGCGAGTTTTCG

Downstream 100 bases:

>100_bases
GCCGTCACCCGTTCAGCCCGTACCTCGCCTAACCCGCCCCGGACCGAAACCACCATGCGTCACGTCATTTCCCTGCTGAT
CGAAAACGAATCCGGCGCGC

Product: acetolactate synthase

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MVLTGAEIVIRCLQEEKVEYVFGYPGGAVLYIYDALFQQEQVRHVLVRHEQAAVHAADGFARSTEKVGVALVTSGPGATN
AVTGIATAFCDSIPMVIISGQVPTAAIGQDAFQEVDTVGITRPCVKHNFLVKDVRDIAATIKKAFYLAKTGRPGPVLVDI
PKDITIAKCDFEYPKEISMRSYSPVVKGHQGQIKKAVQLLLEAKRPMIYTGGGVVLANAAEQLTKLTRLLGFPVTNTLMG
LGGYPASDRQYLGMPGMHGTYEANMAMHYSDVLLAVGARFDDRVIGNPANFAEEPRKIIHIDIDPSSISKRVKVDVPIVG
DLKDVLDEMIRQLEAAPTRPDASNLAAWWKQVEEWRSRKCMEYKNSDEIIKPQFVVQKLWEVTGGDAIVTSDVGQHQMWA
SQYYRFDKPRRWLNSGGLGTMGVGLPYAMGAQFAHPDMPVACVTGEASIQMCIQELSTCKQFRLPIKICNLNNRYLGMVR
QWQQLFHGGRYSESYMDSLPDFAKLASAYGHVGIRVDKPADVEGALREAFTTHKNDLVFLDFQVDPTENVYPMVQGGKGL
TEMILSAEDL

Sequences:

>Translated_570_residues
MVLTGAEIVIRCLQEEKVEYVFGYPGGAVLYIYDALFQQEQVRHVLVRHEQAAVHAADGFARSTEKVGVALVTSGPGATN
AVTGIATAFCDSIPMVIISGQVPTAAIGQDAFQEVDTVGITRPCVKHNFLVKDVRDIAATIKKAFYLAKTGRPGPVLVDI
PKDITIAKCDFEYPKEISMRSYSPVVKGHQGQIKKAVQLLLEAKRPMIYTGGGVVLANAAEQLTKLTRLLGFPVTNTLMG
LGGYPASDRQYLGMPGMHGTYEANMAMHYSDVLLAVGARFDDRVIGNPANFAEEPRKIIHIDIDPSSISKRVKVDVPIVG
DLKDVLDEMIRQLEAAPTRPDASNLAAWWKQVEEWRSRKCMEYKNSDEIIKPQFVVQKLWEVTGGDAIVTSDVGQHQMWA
SQYYRFDKPRRWLNSGGLGTMGVGLPYAMGAQFAHPDMPVACVTGEASIQMCIQELSTCKQFRLPIKICNLNNRYLGMVR
QWQQLFHGGRYSESYMDSLPDFAKLASAYGHVGIRVDKPADVEGALREAFTTHKNDLVFLDFQVDPTENVYPMVQGGKGL
TEMILSAEDL
>Mature_570_residues
MVLTGAEIVIRCLQEEKVEYVFGYPGGAVLYIYDALFQQEQVRHVLVRHEQAAVHAADGFARSTEKVGVALVTSGPGATN
AVTGIATAFCDSIPMVIISGQVPTAAIGQDAFQEVDTVGITRPCVKHNFLVKDVRDIAATIKKAFYLAKTGRPGPVLVDI
PKDITIAKCDFEYPKEISMRSYSPVVKGHQGQIKKAVQLLLEAKRPMIYTGGGVVLANAAEQLTKLTRLLGFPVTNTLMG
LGGYPASDRQYLGMPGMHGTYEANMAMHYSDVLLAVGARFDDRVIGNPANFAEEPRKIIHIDIDPSSISKRVKVDVPIVG
DLKDVLDEMIRQLEAAPTRPDASNLAAWWKQVEEWRSRKCMEYKNSDEIIKPQFVVQKLWEVTGGDAIVTSDVGQHQMWA
SQYYRFDKPRRWLNSGGLGTMGVGLPYAMGAQFAHPDMPVACVTGEASIQMCIQELSTCKQFRLPIKICNLNNRYLGMVR
QWQQLFHGGRYSESYMDSLPDFAKLASAYGHVGIRVDKPADVEGALREAFTTHKNDLVFLDFQVDPTENVYPMVQGGKGL
TEMILSAEDL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=26.8551236749117, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI21361361, Length=505, Percent_Identity=26.1386138613861, Blast_Score=154, Evalue=3e-37,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=57.9225352112676, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=40.9574468085106, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1786717, Length=532, Percent_Identity=33.6466165413534, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI1787096, Length=576, Percent_Identity=27.9513888888889, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1788716, Length=557, Percent_Identity=25.3141831238779, Blast_Score=151, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17542570, Length=516, Percent_Identity=27.7131782945736, Blast_Score=163, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17531299, Length=567, Percent_Identity=27.336860670194, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17531301, Length=567, Percent_Identity=27.336860670194, Blast_Score=156, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6323755, Length=583, Percent_Identity=43.7392795883362, Blast_Score=451, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6320816, Length=498, Percent_Identity=24.8995983935743, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6323163, Length=527, Percent_Identity=23.5294117647059, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19922626, Length=485, Percent_Identity=28.0412371134021, Blast_Score=172, Evalue=5e-43,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62911; Mature: 62911

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLTGAEIVIRCLQEEKVEYVFGYPGGAVLYIYDALFQQEQVRHVLVRHEQAAVHAADGF
CEECCHHHHHHHHHHCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
ARSTEKVGVALVTSGPGATNAVTGIATAFCDSIPMVIISGQVPTAAIGQDAFQEVDTVGI
HCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHCHHHHHHHHHCCC
TRPCVKHNFLVKDVRDIAATIKKAFYLAKTGRPGPVLVDIPKDITIAKCDFEYPKEISMR
CCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCCCEEEECCCCCCCCCCCC
SYSPVVKGHQGQIKKAVQLLLEAKRPMIYTGGGVVLANAAEQLTKLTRLLGFPVTNTLMG
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEHHHHHHHHHHHHHCCCHHHHHHH
LGGYPASDRQYLGMPGMHGTYEANMAMHYSDVLLAVGARFDDRVIGNPANFAEEPRKIIH
CCCCCCCCCCCCCCCCCCCCEECCEEEHHHHHHHHHCCCCCCCCCCCCCCHHHCCCEEEE
IDIDPSSISKRVKVDVPIVGDLKDVLDEMIRQLEAAPTRPDASNLAAWWKQVEEWRSRKC
EEECHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
MEYKNSDEIIKPQFVVQKLWEVTGGDAIVTSDVGQHQMWASQYYRFDKPRRWLNSGGLGT
HCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCCCC
MGVGLPYAMGAQFAHPDMPVACVTGEASIQMCIQELSTCKQFRLPIKICNLNNRYLGMVR
CCCCCCHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHH
QWQQLFHGGRYSESYMDSLPDFAKLASAYGHVGIRVDKPADVEGALREAFTTHKNDLVFL
HHHHHHCCCCCCHHHHHCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCCEEEE
DFQVDPTENVYPMVQGGKGLTEMILSAEDL
EEEECCCCCCCCEEECCCCHHHHHHHCCCC
>Mature Secondary Structure
MVLTGAEIVIRCLQEEKVEYVFGYPGGAVLYIYDALFQQEQVRHVLVRHEQAAVHAADGF
CEECCHHHHHHHHHHCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
ARSTEKVGVALVTSGPGATNAVTGIATAFCDSIPMVIISGQVPTAAIGQDAFQEVDTVGI
HCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHCHHHHHHHHHCCC
TRPCVKHNFLVKDVRDIAATIKKAFYLAKTGRPGPVLVDIPKDITIAKCDFEYPKEISMR
CCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCCCEEEECCCCCCCCCCCC
SYSPVVKGHQGQIKKAVQLLLEAKRPMIYTGGGVVLANAAEQLTKLTRLLGFPVTNTLMG
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCEEEEHHHHHHHHHHHHHCCCHHHHHHH
LGGYPASDRQYLGMPGMHGTYEANMAMHYSDVLLAVGARFDDRVIGNPANFAEEPRKIIH
CCCCCCCCCCCCCCCCCCCCEECCEEEHHHHHHHHHCCCCCCCCCCCCCCHHHCCCEEEE
IDIDPSSISKRVKVDVPIVGDLKDVLDEMIRQLEAAPTRPDASNLAAWWKQVEEWRSRKC
EEECHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
MEYKNSDEIIKPQFVVQKLWEVTGGDAIVTSDVGQHQMWASQYYRFDKPRRWLNSGGLGT
HCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHCCCCCCC
MGVGLPYAMGAQFAHPDMPVACVTGEASIQMCIQELSTCKQFRLPIKICNLNNRYLGMVR
CCCCCCHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHH
QWQQLFHGGRYSESYMDSLPDFAKLASAYGHVGIRVDKPADVEGALREAFTTHKNDLVFL
HHHHHHCCCCCCHHHHHCCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCCEEEE
DFQVDPTENVYPMVQGGKGLTEMILSAEDL
EEEECCCCCCCCEEECCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]