Click here to switch to the map view.

The map label for this gene is purN [H]

Identifier: 119899414

GI number: 119899414

Start: 3432188

End: 3432829

Strand: Reverse

Name: purN [H]

Synonym: azo3124

Alternate gene names: 119899414

Gene position: 3432829-3432188 (Counterclockwise)

Preceding gene: 119899415

Following gene: 119899413

Centisome position: 78.45

GC content: 71.5

Gene sequence:

>642_bases
ATGAAGTCCATCGTCATCCTCATTTCCGGCCGCGGTAGCAATATGGAGGCCATCGTCCGCGCCGGCCTCGACGGCGTCCG
CGTCGCCGCGGTGATCAGCAACCGCCCGGACGCGGCGGGGCTGGCATTCGCCCGCGCGCACGGGATTCCGGTCGCCGTCG
TCGACCACAAGGCCTACCCGGACCGCGCGGCGTTCGATGCCGCGCTCGCCGAAGTCATCGACGCGCACACGCCCGACCTG
GTGGTGCTCGCGGGCTTCATGCGGGTGCTCACCGAGACCTTCGTGCGCCGCTACGAGGGTCGCCTGCTCAACATCCATCC
TTCGCTGCTGCCCGCCTTCCCCGGGCTGCACACGCATCGCCGGGCGCTGGAGGCGGGGGTGAAGGTCCATGGCGCCACGG
TGCATTTCGTCACCGCCGATCTCGATTGCGGCCCCATCGTCGTTCAGGCGGTGGTGCCGGTGCTGGCCGACGACGACGAG
GCGGCGCTCGCCGCCCGCGTGCTGGCGCAGGAACACCGCATCTACCCGCAAGCCCTGCGTTGGTTCGCCGCCGGCCGCTT
GAGCCTGGAGGCAGGCCGGGTGCGGCTGCGCGACGAACCGGCGACGAGCGCGGGCTGCGTCGTGCCGCCGGTGGAAGGCT
AG

Upstream 100 bases:

>100_bases
ACGCACGGTCACCCGCGTGGTCCAGAACATGGCCACGGCGGTCTTTCTGCCGGTGCGCTGAAGCGCGCCGCATCCGCAAC
AGCTTCCCGTCCATAGGCTC

Downstream 100 bases:

>100_bases
GCGGACATGAGCAAGCGCGCGATGGCCGCTGCACTGACGGTGTCGGTGCTCGCCCATGCGCTGGTGCTCGGCGGCGCCGA
ATGGCGCTGGCCGAGCGCGC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKSIVILISGRGSNMEAIVRAGLDGVRVAAVISNRPDAAGLAFARAHGIPVAVVDHKAYPDRAAFDAALAEVIDAHTPDL
VVLAGFMRVLTETFVRRYEGRLLNIHPSLLPAFPGLHTHRRALEAGVKVHGATVHFVTADLDCGPIVVQAVVPVLADDDE
AALAARVLAQEHRIYPQALRWFAAGRLSLEAGRVRLRDEPATSAGCVVPPVEG

Sequences:

>Translated_213_residues
MKSIVILISGRGSNMEAIVRAGLDGVRVAAVISNRPDAAGLAFARAHGIPVAVVDHKAYPDRAAFDAALAEVIDAHTPDL
VVLAGFMRVLTETFVRRYEGRLLNIHPSLLPAFPGLHTHRRALEAGVKVHGATVHFVTADLDCGPIVVQAVVPVLADDDE
AALAARVLAQEHRIYPQALRWFAAGRLSLEAGRVRLRDEPATSAGCVVPPVEG
>Mature_213_residues
MKSIVILISGRGSNMEAIVRAGLDGVRVAAVISNRPDAAGLAFARAHGIPVAVVDHKAYPDRAAFDAALAEVIDAHTPDL
VVLAGFMRVLTETFVRRYEGRLLNIHPSLLPAFPGLHTHRRALEAGVKVHGATVHFVTADLDCGPIVVQAVVPVLADDDE
AALAARVLAQEHRIYPQALRWFAAGRLSLEAGRVRLRDEPATSAGCVVPPVEG

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=195, Percent_Identity=45.6410256410256, Blast_Score=176, Evalue=1e-44,
Organism=Homo sapiens, GI209869995, Length=195, Percent_Identity=45.6410256410256, Blast_Score=176, Evalue=1e-44,
Organism=Homo sapiens, GI209869993, Length=195, Percent_Identity=45.6410256410256, Blast_Score=176, Evalue=1e-44,
Organism=Escherichia coli, GI1788846, Length=190, Percent_Identity=54.2105263157895, Blast_Score=200, Evalue=7e-53,
Organism=Escherichia coli, GI1787483, Length=156, Percent_Identity=41.025641025641, Blast_Score=106, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17567511, Length=184, Percent_Identity=39.6739130434783, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6320616, Length=199, Percent_Identity=29.1457286432161, Blast_Score=75, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24582400, Length=189, Percent_Identity=48.1481481481481, Blast_Score=175, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22660; Mature: 22660

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIVILISGRGSNMEAIVRAGLDGVRVAAVISNRPDAAGLAFARAHGIPVAVVDHKAYP
CCEEEEEEECCCCCHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCC
DRAAFDAALAEVIDAHTPDLVVLAGFMRVLTETFVRRYEGRLLNIHPSLLPAFPGLHTHR
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHCCCCHHHHH
RALEAGVKVHGATVHFVTADLDCGPIVVQAVVPVLADDDEAALAARVLAQEHRIYPQALR
HHHHCCCEEECEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHH
WFAAGRLSLEAGRVRLRDEPATSAGCVVPPVEG
HHHHCCEEEECCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSIVILISGRGSNMEAIVRAGLDGVRVAAVISNRPDAAGLAFARAHGIPVAVVDHKAYP
CCEEEEEEECCCCCHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCC
DRAAFDAALAEVIDAHTPDLVVLAGFMRVLTETFVRRYEGRLLNIHPSLLPAFPGLHTHR
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHCCCCHHHHH
RALEAGVKVHGATVHFVTADLDCGPIVVQAVVPVLADDDEAALAARVLAQEHRIYPQALR
HHHHCCCEEECEEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHH
WFAAGRLSLEAGRVRLRDEPATSAGCVVPPVEG
HHHHCCEEEECCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]