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The map label for this gene is livJ [C]

Identifier: 119899340

GI number: 119899340

Start: 3353237

End: 3354418

Strand: Reverse

Name: livJ [C]

Synonym: azo3050

Alternate gene names: 119899340

Gene position: 3354418-3353237 (Counterclockwise)

Preceding gene: 119899341

Following gene: 119899339

Centisome position: 76.65

GC content: 66.75

Gene sequence:

>1182_bases
ATGGAAAAGCAGAATCGCAAGGCAACGCGCGTGGTGGCCGTCCTGGCCGGTCTGGGTCTGGCCACGCTGATGCCGCTGGC
CGCCCATGCCGCCGAGAAGGTGAAGGTCGGCCTGATGCTGCCCTACACCGGCACCTACGCCGCGCTCGGCAACGCGATCA
CCAACGGCTTCAAGCAGTACGTGGCGGAGCAGGGCGGCACGCTCGCCGGCCGCGAGGTCGAGTACTTCGTGGTGGATGAC
GAATCCGATCCCGCCAAGGCCACCGAGAACACCAACAAGCTGGTCAAGCGCGACGAGGTGGATGTGCTGGTCGGCACGGT
GCACTCGGGCGTTGCGCTGGCGATGGCCAAGGTCGCGCGCGACACCAAGACGCTGATGATCATCCCCAACGCCGGCGCCG
ACGAACTCACCGGCCCGCTGTGTGCGCCCAACGTGTTCCGCACCTCCTTCACCAACTGGCAGCCGGCCTACGCCATGGGC
AAGGTCGCCGCGGAGAAGGGCCACAAGACGGCGGTCACGCTGACCTGGAAGTACGCCGCGGGCGAACAGGCGGTGGAAGG
CTTCGCCAAGGCCTTCGAGGCGGGCGGCGGCAAGGTCGTCAAGCAGCTCACGCTGCCCTTCCCGAACGTGGAGTTCCAGG
CCCTGCTGACCGAAGTCGCCTCGCTCAAGCCGGACGCGGTGTACGTGTTCTTTGCCGGCGGCGGCGCCGCCAAGTTCGTC
AAGGACTACGACGCGGCCGGGCTGAAGAAGAGCATTCCGCTGTACTCCAGCGGCTTCCTCACCGACGGCACGCTGGAGGC
CATGGGCGGTGCCGGCGAAGGTCTGCTGACCGCGCTGCACTACGCCGACGGCCTGGAAATCCCGAAGGACAAGGACTTCC
GCACCAAGTACGCGATGACCTACAAGATGCAGCCCGACGTCTATGCGGTGCAGGGCTATGACGCGGCGCAGCTGCTGAAG
TCGGGCCTGGACGCGGTCAAGGGCGGCAAGCTCGACCAGGCGACCATGGTCAAGGCCATGGAAGCGGCCACCATCGACAG
CCCGCGCGGCGCCTTCACGCTGTCCAAGGCCCACAACCCGGTGCAGGACATCTACCTGCGCAAGGCCGAAGGCAAGGACA
ACAAGCTGGTGAACGTCGCGGTCAAGAAGCTGGCCGACCCGGCGCGCGGCTGCCGCATGTAA

Upstream 100 bases:

>100_bases
GCCTTTCAGGCCAACCGGAGCCAGGCCTGGTAAGGGTCTGGAAAGCAGGGCCACGGGCGGCGGCAACAGCCGCCCGTTCA
TCAAAAACGAGGAGACAACG

Downstream 100 bases:

>100_bases
GGACCGCGGTCCGCCGCGTCCTTCGACGCGGTGGTCCGGTGCCAGCACCGTTCGCCCCGAGCCCGTCGAAGGGCAGCCCG
GCTCCCGGGCTTCGACAGGC

Product: putative leucine-specific binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MEKQNRKATRVVAVLAGLGLATLMPLAAHAAEKVKVGLMLPYTGTYAALGNAITNGFKQYVAEQGGTLAGREVEYFVVDD
ESDPAKATENTNKLVKRDEVDVLVGTVHSGVALAMAKVARDTKTLMIIPNAGADELTGPLCAPNVFRTSFTNWQPAYAMG
KVAAEKGHKTAVTLTWKYAAGEQAVEGFAKAFEAGGGKVVKQLTLPFPNVEFQALLTEVASLKPDAVYVFFAGGGAAKFV
KDYDAAGLKKSIPLYSSGFLTDGTLEAMGGAGEGLLTALHYADGLEIPKDKDFRTKYAMTYKMQPDVYAVQGYDAAQLLK
SGLDAVKGGKLDQATMVKAMEAATIDSPRGAFTLSKAHNPVQDIYLRKAEGKDNKLVNVAVKKLADPARGCRM

Sequences:

>Translated_393_residues
MEKQNRKATRVVAVLAGLGLATLMPLAAHAAEKVKVGLMLPYTGTYAALGNAITNGFKQYVAEQGGTLAGREVEYFVVDD
ESDPAKATENTNKLVKRDEVDVLVGTVHSGVALAMAKVARDTKTLMIIPNAGADELTGPLCAPNVFRTSFTNWQPAYAMG
KVAAEKGHKTAVTLTWKYAAGEQAVEGFAKAFEAGGGKVVKQLTLPFPNVEFQALLTEVASLKPDAVYVFFAGGGAAKFV
KDYDAAGLKKSIPLYSSGFLTDGTLEAMGGAGEGLLTALHYADGLEIPKDKDFRTKYAMTYKMQPDVYAVQGYDAAQLLK
SGLDAVKGGKLDQATMVKAMEAATIDSPRGAFTLSKAHNPVQDIYLRKAEGKDNKLVNVAVKKLADPARGCRM
>Mature_393_residues
MEKQNRKATRVVAVLAGLGLATLMPLAAHAAEKVKVGLMLPYTGTYAALGNAITNGFKQYVAEQGGTLAGREVEYFVVDD
ESDPAKATENTNKLVKRDEVDVLVGTVHSGVALAMAKVARDTKTLMIIPNAGADELTGPLCAPNVFRTSFTNWQPAYAMG
KVAAEKGHKTAVTLTWKYAAGEQAVEGFAKAFEAGGGKVVKQLTLPFPNVEFQALLTEVASLKPDAVYVFFAGGGAAKFV
KDYDAAGLKKSIPLYSSGFLTDGTLEAMGGAGEGLLTALHYADGLEIPKDKDFRTKYAMTYKMQPDVYAVQGYDAAQLLK
SGLDAVKGGKLDQATMVKAMEAATIDSPRGAFTLSKAHNPVQDIYLRKAEGKDNKLVNVAVKKLADPARGCRM

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

None

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41754; Mature: 41754

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKQNRKATRVVAVLAGLGLATLMPLAAHAAEKVKVGLMLPYTGTYAALGNAITNGFKQY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHH
VAEQGGTLAGREVEYFVVDDESDPAKATENTNKLVKRDEVDVLVGTVHSGVALAMAKVAR
HHHCCCCEECCEEEEEEEECCCCCCCCCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHC
DTKTLMIIPNAGADELTGPLCAPNVFRTSFTNWQPAYAMGKVAAEKGHKTAVTLTWKYAA
CCEEEEEEECCCCHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECC
GEQAVEGFAKAFEAGGGKVVKQLTLPFPNVEFQALLTEVASLKPDAVYVFFAGGGAAKFV
CHHHHHHHHHHHHCCCCCEEHEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHH
KDYDAAGLKKSIPLYSSGFLTDGTLEAMGGAGEGLLTALHYADGLEIPKDKDFRTKYAMT
HHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEE
YKMQPDVYAVQGYDAAQLLKSGLDAVKGGKLDQATMVKAMEAATIDSPRGAFTLSKAHNP
EEECCCEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCC
VQDIYLRKAEGKDNKLVNVAVKKLADPARGCRM
HHHHHEEECCCCCCHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure
MEKQNRKATRVVAVLAGLGLATLMPLAAHAAEKVKVGLMLPYTGTYAALGNAITNGFKQY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHHH
VAEQGGTLAGREVEYFVVDDESDPAKATENTNKLVKRDEVDVLVGTVHSGVALAMAKVAR
HHHCCCCEECCEEEEEEEECCCCCCCCCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHC
DTKTLMIIPNAGADELTGPLCAPNVFRTSFTNWQPAYAMGKVAAEKGHKTAVTLTWKYAA
CCEEEEEEECCCCHHHCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECC
GEQAVEGFAKAFEAGGGKVVKQLTLPFPNVEFQALLTEVASLKPDAVYVFFAGGGAAKFV
CHHHHHHHHHHHHCCCCCEEHEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHH
KDYDAAGLKKSIPLYSSGFLTDGTLEAMGGAGEGLLTALHYADGLEIPKDKDFRTKYAMT
HHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEE
YKMQPDVYAVQGYDAAQLLKSGLDAVKGGKLDQATMVKAMEAATIDSPRGAFTLSKAHNP
EEECCCEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCC
VQDIYLRKAEGKDNKLVNVAVKKLADPARGCRM
HHHHHEEECCCCCCHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA