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The map label for this gene is xthA [H]

Identifier: 119899170

GI number: 119899170

Start: 3162198

End: 3162959

Strand: Direct

Name: xthA [H]

Synonym: azo2880

Alternate gene names: 119899170

Gene position: 3162198-3162959 (Clockwise)

Preceding gene: 119899168

Following gene: 119899171

Centisome position: 72.26

GC content: 67.59

Gene sequence:

>762_bases
ATGAAAATCGCTACCTGGAACGTCAACTCCCTCAAGGTCCGCCTGCCCCACGTCCTCGACTGGCTGGCGGCGAACCAGCC
CGATGCGCTCTGCCTGCAGGAGCTGAAGCTCGAAGACAAGGGCTTTCCGGTCGCCGAACTGCAGGCCGCGGGCTACAACG
CCGTCTTCAACGGCCAGAAGACCTATAACGGCGTCGCCATCCTCAGCCCGCACCCGCTGGAAGACGTGGTGCGCGACATC
CCCGGCTTTTCGGACGAACAGAAGCGCATCATCGCGGCCACGGTGAACGGCGTGCGCATCGTCTGCGGCTACTTTCCCAA
CGGCCAGGCGGTGGGCTCGGACAAGTTCGAATACAAGCTGCGCTGGCTGGGGGCGCTGACCGAATGGCTGCGCGCGGAAC
TCACGGCGCATCCGCGGCTGGTGCTGGGGGGCGACTTCAACATCGCCCCGGAGGACCGCGACGCGCACCCCGACTGGAAG
GACGAGATCCACGTCTCGGCGCCGGAGCGCGCCGCCTTCACCGCGCTGACCGCGCTCGGACTGGTCGATGCGTTCCGCCT
CTACGACCAGCCGGAGCGGGCGTTCTCGTGGTGGGACTACCGCATGGGCGCATTCCGGCGCAATTTCGGCCTGCGCATCG
ACCACCTGCTGGTATCGCCCGCGCTGCAGCCGGCGTGCCGGGCCTGCACGGTGGACAAGGCCCCGCGCAAGCTGGAGCGC
CCATCCGACCATGCCCCGGTCGTGCTCGACCTGGAAGTCTGA

Upstream 100 bases:

>100_bases
GGAGCGGCCGCGGGTTGCGCTACTATCCGGGCCATTCCGGAATCCGCCGCCCGGGGCCACCCGCCCAGGCGCGGCGGCCC
GCCCTCGATCGGAACCCCTG

Downstream 100 bases:

>100_bases
GGCGCGTGCGACAGGCGGACACCGGCGTCGTGCTCGCCCACGGCAAGTGGGACCGGCGCCCGTTCGCCATCGACGCCCTC
GCGACACCGCTTGCAACGGC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MKIATWNVNSLKVRLPHVLDWLAANQPDALCLQELKLEDKGFPVAELQAAGYNAVFNGQKTYNGVAILSPHPLEDVVRDI
PGFSDEQKRIIAATVNGVRIVCGYFPNGQAVGSDKFEYKLRWLGALTEWLRAELTAHPRLVLGGDFNIAPEDRDAHPDWK
DEIHVSAPERAAFTALTALGLVDAFRLYDQPERAFSWWDYRMGAFRRNFGLRIDHLLVSPALQPACRACTVDKAPRKLER
PSDHAPVVLDLEV

Sequences:

>Translated_253_residues
MKIATWNVNSLKVRLPHVLDWLAANQPDALCLQELKLEDKGFPVAELQAAGYNAVFNGQKTYNGVAILSPHPLEDVVRDI
PGFSDEQKRIIAATVNGVRIVCGYFPNGQAVGSDKFEYKLRWLGALTEWLRAELTAHPRLVLGGDFNIAPEDRDAHPDWK
DEIHVSAPERAAFTALTALGLVDAFRLYDQPERAFSWWDYRMGAFRRNFGLRIDHLLVSPALQPACRACTVDKAPRKLER
PSDHAPVVLDLEV
>Mature_253_residues
MKIATWNVNSLKVRLPHVLDWLAANQPDALCLQELKLEDKGFPVAELQAAGYNAVFNGQKTYNGVAILSPHPLEDVVRDI
PGFSDEQKRIIAATVNGVRIVCGYFPNGQAVGSDKFEYKLRWLGALTEWLRAELTAHPRLVLGGDFNIAPEDRDAHPDWK
DEIHVSAPERAAFTALTALGLVDAFRLYDQPERAFSWWDYRMGAFRRNFGLRIDHLLVSPALQPACRACTVDKAPRKLER
PSDHAPVVLDLEV

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=262, Percent_Identity=34.3511450381679, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI18375503, Length=262, Percent_Identity=34.3511450381679, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI18375501, Length=262, Percent_Identity=34.3511450381679, Blast_Score=113, Evalue=2e-25,
Organism=Escherichia coli, GI1788046, Length=264, Percent_Identity=38.2575757575758, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71989536, Length=261, Percent_Identity=26.4367816091954, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221330655, Length=266, Percent_Identity=28.9473684210526, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17136678, Length=266, Percent_Identity=28.9473684210526, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 28364; Mature: 28364

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATWNVNSLKVRLPHVLDWLAANQPDALCLQELKLEDKGFPVAELQAAGYNAVFNGQK
CEEEEECCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCEEECCCC
TYNGVAILSPHPLEDVVRDIPGFSDEQKRIIAATVNGVRIVCGYFPNGQAVGSDKFEYKL
CCCCEEEECCCCHHHHHHHCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCHHHHH
RWLGALTEWLRAELTAHPRLVLGGDFNIAPEDRDAHPDWKDEIHVSAPERAAFTALTALG
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHH
LVDAFRLYDQPERAFSWWDYRMGAFRRNFGLRIDHLLVSPALQPACRACTVDKAPRKLER
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCHHHHHHHCCCCCCCHHHCC
PSDHAPVVLDLEV
CCCCCCEEEEEEC
>Mature Secondary Structure
MKIATWNVNSLKVRLPHVLDWLAANQPDALCLQELKLEDKGFPVAELQAAGYNAVFNGQK
CEEEEECCCEEEEEHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHCCCCCEEECCCC
TYNGVAILSPHPLEDVVRDIPGFSDEQKRIIAATVNGVRIVCGYFPNGQAVGSDKFEYKL
CCCCEEEECCCCHHHHHHHCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCCCCCHHHHH
RWLGALTEWLRAELTAHPRLVLGGDFNIAPEDRDAHPDWKDEIHVSAPERAAFTALTALG
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHH
LVDAFRLYDQPERAFSWWDYRMGAFRRNFGLRIDHLLVSPALQPACRACTVDKAPRKLER
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCHHHHHHHCCCCCCCHHHCC
PSDHAPVVLDLEV
CCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]