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The map label for this gene is rsmE [H]

Identifier: 119899122

GI number: 119899122

Start: 3111292

End: 3112023

Strand: Reverse

Name: rsmE [H]

Synonym: azo2832

Alternate gene names: 119899122

Gene position: 3112023-3111292 (Counterclockwise)

Preceding gene: 119899130

Following gene: 119899121

Centisome position: 71.12

GC content: 71.31

Gene sequence:

>732_bases
ATGATTTCACGATTCCATTTTCCGGGCGTGCTGCCCCGGCACGGCGAGACGGCGTTGCCCGACGCGGTCGCCCACCACGC
AACGCGGGTGTTGAGGATGCGCGATGGCGAACCCATCGTGCTGTTTGACGGGAGTGGGGCGGAGGCGGAGGCCACGCTGG
TGCTGCGCGGCAAGTCCGCCGCGGCGCTGATTTCCGCCTATCGCGAGGTGGACCGCGAGTCGCCGCTGGACCTGGTGCTG
GTGCAGGCGCTGGCAACCGGCGACAAGATGGACTGGATCGTGCAGAAAGCGGTGGAACTCGGCGCCCGCGCGGTTCAGCC
CATCCAGGCCGAGCGTTCGGTGTTGCGGCTGGCGGGCGAGCGCGCGACGCGGCGGCGCGAGCACTGGCAGCAGGTTGCCG
TGGGTGCCTGCGAACAAAGCGGGCGCAACCGCATTCCCGAAGTCGGCGAAATCCTTTCGCTGCAGGCTTATCTCGCCGCC
GCGCGCGAGGACCGCGCGACGGTTCGCTGCGTGCTCGACCCGGCCGCCGCACAGGGGCTGTCCGCGCTGCCGCGCCCGGC
GGGTGCGCTGCATCTGCTGGTGGGGCCCGAAGGCGGCTGGAGCGACGATGAACTCGCCGCCTGTGCAGCGGCGCACTGCA
CCCCGGTCGGCCTCGGTCCGCGTGTGCTGCGCACCGAAACCGCCGGGCTCGCGATGCTGGCGGCGCTGCAGGCGCGCTGG
GGTGACTTCTGA

Upstream 100 bases:

>100_bases
CGAGGGATTCGAGCGCCCGGGCGCGGGCGAGACGTTGGGCTGCATTCGGCATGGGTGGTCCGCGGGCTGATCGGCCTAGG
GCTATGCCTATAATCTTACG

Downstream 100 bases:

>100_bases
CACCTTTCCCTCAATCACCTTACTAATCAGGAATCGCAGCGGCATGTTCGAGTCGGCGGAACTCGGTCACAAGACCACCA
AGGAAGACTACGAGGCGGCG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MISRFHFPGVLPRHGETALPDAVAHHATRVLRMRDGEPIVLFDGSGAEAEATLVLRGKSAAALISAYREVDRESPLDLVL
VQALATGDKMDWIVQKAVELGARAVQPIQAERSVLRLAGERATRRREHWQQVAVGACEQSGRNRIPEVGEILSLQAYLAA
AREDRATVRCVLDPAAAQGLSALPRPAGALHLLVGPEGGWSDDELAACAAAHCTPVGLGPRVLRTETAGLAMLAALQARW
GDF

Sequences:

>Translated_243_residues
MISRFHFPGVLPRHGETALPDAVAHHATRVLRMRDGEPIVLFDGSGAEAEATLVLRGKSAAALISAYREVDRESPLDLVL
VQALATGDKMDWIVQKAVELGARAVQPIQAERSVLRLAGERATRRREHWQQVAVGACEQSGRNRIPEVGEILSLQAYLAA
AREDRATVRCVLDPAAAQGLSALPRPAGALHLLVGPEGGWSDDELAACAAAHCTPVGLGPRVLRTETAGLAMLAALQARW
GDF
>Mature_243_residues
MISRFHFPGVLPRHGETALPDAVAHHATRVLRMRDGEPIVLFDGSGAEAEATLVLRGKSAAALISAYREVDRESPLDLVL
VQALATGDKMDWIVQKAVELGARAVQPIQAERSVLRLAGERATRRREHWQQVAVGACEQSGRNRIPEVGEILSLQAYLAA
AREDRATVRCVLDPAAAQGLSALPRPAGALHLLVGPEGGWSDDELAACAAAHCTPVGLGPRVLRTETAGLAMLAALQARW
GDF

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=39.917695473251, Blast_Score=183, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 25963; Mature: 25963

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRFHFPGVLPRHGETALPDAVAHHATRVLRMRDGEPIVLFDGSGAEAEATLVLRGKSA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCEEEEECCCCCCCEEEEEEECCHH
AALISAYREVDRESPLDLVLVQALATGDKMDWIVQKAVELGARAVQPIQAERSVLRLAGE
HHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
RATRRREHWQQVAVGACEQSGRNRIPEVGEILSLQAYLAAAREDRATVRCVLDPAAAQGL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHCH
SALPRPAGALHLLVGPEGGWSDDELAACAAAHCTPVGLGPRVLRTETAGLAMLAALQARW
HCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GDF
CCC
>Mature Secondary Structure
MISRFHFPGVLPRHGETALPDAVAHHATRVLRMRDGEPIVLFDGSGAEAEATLVLRGKSA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCEEEEECCCCCCCEEEEEEECCHH
AALISAYREVDRESPLDLVLVQALATGDKMDWIVQKAVELGARAVQPIQAERSVLRLAGE
HHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
RATRRREHWQQVAVGACEQSGRNRIPEVGEILSLQAYLAAAREDRATVRCVLDPAAAQGL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHCH
SALPRPAGALHLLVGPEGGWSDDELAACAAAHCTPVGLGPRVLRTETAGLAMLAALQARW
HCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GDF
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]