Click here to switch to the map view.

The map label for this gene is glyA [H]

Identifier: 119899091

GI number: 119899091

Start: 3084401

End: 3085651

Strand: Direct

Name: glyA [H]

Synonym: azo2801

Alternate gene names: 119899091

Gene position: 3084401-3085651 (Clockwise)

Preceding gene: 119899090

Following gene: 119899092

Centisome position: 70.48

GC content: 66.11

Gene sequence:

>1251_bases
ATGTTCTCCTCGCAAGACACTCTCGCCAAGGTCGATCCCGAACTCTGGTCCGCCATCCAGGCGGAGAACCGCCGCCAGGA
AGATCACATCGAGCTGATCGCCTCCGAAAACTACGTCTCCCACGCGGTGATGGAAGCCCAGGGCTCCCAGCTCACCAACA
AGTACGCGGAAGGCTATCCGGGCAAGCGCTACTACGGCGGCTGTGAACATGTGGACGTGGTCGAGCAGCTCGCGATCGAC
CGTCTGAAGAAGCTCTTCGGCGCCGACGCCGCCAACGTGCAGCCGAACTCCGGCTCCCAGGCCAACCAGGCGGTGCTGAT
GGCCTTCGCCAAGCCCGGCGACACCATCATGGGCATGAGCCTGGCCGAAGGCGGCCACCTCACCCACGGCATGCCGCTCA
ACATGTCGGGCAAATGGTTCAACGTCGTCGCCTACGGCCTCGACGAGAAGGAAGAGATCAACTACGCGGCGATGGAAGCG
CTCGCCCGCGAGCACAAGCCGAAGATCATCATCGCCGGCGCCTCCGCCTATGCCCTGCGCATCGACTTCGAGCGCTTCGC
GCGCATCGCGCGTGAAGTGGGCGCCATCTTCTGGGTGGACATGGCCCACTACGCCGGCCTGATCGCCGCGGGCTACTACC
CCAACCCGGTGCCGCACGCCGACGTGGTCACCTCCACCACCCACAAGACCCTGCGCGGCCCGCGCGGCGGCATCATCCTG
ATGAAGGCCGAGCACGAGAAGGCGATCAACTCCGCGATCTTCCCCGGCCTGCAGGGCGGCCCGCTGGAGCACGTCATCGC
CGCCAAGGCGGTGGCCTTCAAGGAAGCCGCCACCCCGGCGTTCCGCGACTACCAGGAACAGGTCATCGCCAACGCCCGCG
TGATGGCGCGCGTGCTCGGCGAAGAACGCGGCCTGCGCATCGTCTCCGGCCGCACCGAGTCCCACGTCTTCCTGGTCGAC
CTGCGCTCCAAGAACATCACCGGCAAGGAAGCCGAAGCCGTGCTGGGCAGCGCCCACATCACGGTCAACAAGAACTCGAT
CCCCAACGATCCGCAGAAGCCCTTCGTCACTTCCGGCATCCGCATCGGTTCGCCGGCGATGACCACCCGCGGCTTCACCG
AGATCGAAGCCGAGCAGATCGCCCACCTGATCGCTGACGTGCTCGACGCCCCGCAGGACGCGGCGGTCCTCGAACGCGTG
CGCGGCAAGGTCGGCGAGCTGTGCGCCAAGTTCCCGGTCTACGGTAGCTAA

Upstream 100 bases:

>100_bases
CGCGGGGCCAACACGCGCCGGCGCATCGCCATCGGCGGTTTCGCGCACAGTGCGTGGTAACATCCGCTTTTAAAAACCAC
TCGTTACCGAGCCCGCGTCC

Downstream 100 bases:

>100_bases
GGCAACGAGGGCCGGCCCAAGCGCCGGCCCGACCCGCCGACATGAAGTGCCCCTTCTGCGACGACCCCAACACCCAGGTC
ACCGACACCCGTGAGAACGA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MFSSQDTLAKVDPELWSAIQAENRRQEDHIELIASENYVSHAVMEAQGSQLTNKYAEGYPGKRYYGGCEHVDVVEQLAID
RLKKLFGADAANVQPNSGSQANQAVLMAFAKPGDTIMGMSLAEGGHLTHGMPLNMSGKWFNVVAYGLDEKEEINYAAMEA
LAREHKPKIIIAGASAYALRIDFERFARIAREVGAIFWVDMAHYAGLIAAGYYPNPVPHADVVTSTTHKTLRGPRGGIIL
MKAEHEKAINSAIFPGLQGGPLEHVIAAKAVAFKEAATPAFRDYQEQVIANARVMARVLGEERGLRIVSGRTESHVFLVD
LRSKNITGKEAEAVLGSAHITVNKNSIPNDPQKPFVTSGIRIGSPAMTTRGFTEIEAEQIAHLIADVLDAPQDAAVLERV
RGKVGELCAKFPVYGS

Sequences:

>Translated_416_residues
MFSSQDTLAKVDPELWSAIQAENRRQEDHIELIASENYVSHAVMEAQGSQLTNKYAEGYPGKRYYGGCEHVDVVEQLAID
RLKKLFGADAANVQPNSGSQANQAVLMAFAKPGDTIMGMSLAEGGHLTHGMPLNMSGKWFNVVAYGLDEKEEINYAAMEA
LAREHKPKIIIAGASAYALRIDFERFARIAREVGAIFWVDMAHYAGLIAAGYYPNPVPHADVVTSTTHKTLRGPRGGIIL
MKAEHEKAINSAIFPGLQGGPLEHVIAAKAVAFKEAATPAFRDYQEQVIANARVMARVLGEERGLRIVSGRTESHVFLVD
LRSKNITGKEAEAVLGSAHITVNKNSIPNDPQKPFVTSGIRIGSPAMTTRGFTEIEAEQIAHLIADVLDAPQDAAVLERV
RGKVGELCAKFPVYGS
>Mature_416_residues
MFSSQDTLAKVDPELWSAIQAENRRQEDHIELIASENYVSHAVMEAQGSQLTNKYAEGYPGKRYYGGCEHVDVVEQLAID
RLKKLFGADAANVQPNSGSQANQAVLMAFAKPGDTIMGMSLAEGGHLTHGMPLNMSGKWFNVVAYGLDEKEEINYAAMEA
LAREHKPKIIIAGASAYALRIDFERFARIAREVGAIFWVDMAHYAGLIAAGYYPNPVPHADVVTSTTHKTLRGPRGGIIL
MKAEHEKAINSAIFPGLQGGPLEHVIAAKAVAFKEAATPAFRDYQEQVIANARVMARVLGEERGLRIVSGRTESHVFLVD
LRSKNITGKEAEAVLGSAHITVNKNSIPNDPQKPFVTSGIRIGSPAMTTRGFTEIEAEQIAHLIADVLDAPQDAAVLERV
RGKVGELCAKFPVYGS

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=408, Percent_Identity=46.5686274509804, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI261862352, Length=408, Percent_Identity=46.5686274509804, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI261862350, Length=408, Percent_Identity=46.5686274509804, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI261862348, Length=408, Percent_Identity=46.5686274509804, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI261862346, Length=407, Percent_Identity=45.945945945946, Blast_Score=335, Evalue=5e-92,
Organism=Homo sapiens, GI22547186, Length=414, Percent_Identity=44.4444444444444, Blast_Score=311, Evalue=9e-85,
Organism=Homo sapiens, GI22547189, Length=399, Percent_Identity=42.3558897243108, Blast_Score=276, Evalue=3e-74,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=63.5491606714628, Blast_Score=531, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI25144732, Length=403, Percent_Identity=44.6650124069479, Blast_Score=332, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI25144729, Length=403, Percent_Identity=44.6650124069479, Blast_Score=331, Evalue=4e-91,
Organism=Saccharomyces cerevisiae, GI6319739, Length=412, Percent_Identity=42.7184466019417, Blast_Score=336, Evalue=5e-93,
Organism=Saccharomyces cerevisiae, GI6323087, Length=432, Percent_Identity=42.5925925925926, Blast_Score=322, Evalue=5e-89,
Organism=Drosophila melanogaster, GI24640005, Length=458, Percent_Identity=41.4847161572052, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI221329721, Length=458, Percent_Identity=41.4847161572052, Blast_Score=338, Evalue=3e-93,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45173; Mature: 45173

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSSQDTLAKVDPELWSAIQAENRRQEDHIELIASENYVSHAVMEAQGSQLTNKYAEGYP
CCCCCCCHHHCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHCCCC
GKRYYGGCEHVDVVEQLAIDRLKKLFGADAANVQPNSGSQANQAVLMAFAKPGDTIMGMS
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
LAEGGHLTHGMPLNMSGKWFNVVAYGLDEKEEINYAAMEALAREHKPKIIIAGASAYALR
ECCCCCCCCCCCCCCCCCEEEEEEECCCCHHHCCHHHHHHHHHHCCCCEEEECCCEEEEE
IDFERFARIAREVGAIFWVDMAHYAGLIAAGYYPNPVPHADVVTSTTHKTLRGPRGGIIL
ECHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEECCHHHHHCCCCCCEEE
MKAEHEKAINSAIFPGLQGGPLEHVIAAKAVAFKEAATPAFRDYQEQVIANARVMARVLG
EECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
EERGLRIVSGRTESHVFLVDLRSKNITGKEAEAVLGSAHITVNKNSIPNDPQKPFVTSGI
HHCCEEEEECCCCCEEEEEEECCCCCCCCHHHHHHCEEEEEEECCCCCCCCCCCCCCCCE
RIGSPAMTTRGFTEIEAEQIAHLIADVLDAPQDAAVLERVRGKVGELCAKFPVYGS
EECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFSSQDTLAKVDPELWSAIQAENRRQEDHIELIASENYVSHAVMEAQGSQLTNKYAEGYP
CCCCCCCHHHCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHCCCC
GKRYYGGCEHVDVVEQLAIDRLKKLFGADAANVQPNSGSQANQAVLMAFAKPGDTIMGMS
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEE
LAEGGHLTHGMPLNMSGKWFNVVAYGLDEKEEINYAAMEALAREHKPKIIIAGASAYALR
ECCCCCCCCCCCCCCCCCEEEEEEECCCCHHHCCHHHHHHHHHHCCCCEEEECCCEEEEE
IDFERFARIAREVGAIFWVDMAHYAGLIAAGYYPNPVPHADVVTSTTHKTLRGPRGGIIL
ECHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEECCHHHHHCCCCCCEEE
MKAEHEKAINSAIFPGLQGGPLEHVIAAKAVAFKEAATPAFRDYQEQVIANARVMARVLG
EECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
EERGLRIVSGRTESHVFLVDLRSKNITGKEAEAVLGSAHITVNKNSIPNDPQKPFVTSGI
HHCCEEEEECCCCCEEEEEEECCCCCCCCHHHHHHCEEEEEEECCCCCCCCCCCCCCCCE
RIGSPAMTTRGFTEIEAEQIAHLIADVLDAPQDAAVLERVRGKVGELCAKFPVYGS
EECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA