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The map label for this gene is glpK

Identifier: 119899041

GI number: 119899041

Start: 3033471

End: 3034961

Strand: Reverse

Name: glpK

Synonym: azo2751

Alternate gene names: 119899041

Gene position: 3034961-3033471 (Counterclockwise)

Preceding gene: 119899045

Following gene: 119899040

Centisome position: 69.35

GC content: 72.43

Gene sequence:

>1491_bases
ATGAGCTACGTGCTGGCCCTCGACCAAGGCACGACGAGTTCGCGCGCGATGGTCTTCGACGCTGCCGGCAGGGTACGCAG
CGTCGCCCGGCGCGACTTCACCCAGTCCTTCCCGCAGCCCGGCTGGGTGGAGCACGACCCGGCGGAAATCCTGCGCAGCC
AGTTCGACTGCGCGCGCGATGCGTTGGCGGATGCCGGGCTGCGTGCGGGCCAGCTGGCCGCCATCGGCATCGCCAACCAG
CGCGAAACCACCGTGCTGTGGGAGCGCGCCAGCGGTCGCGCGCTGGCGCCGGCCATCGTGTGGCAGGACCGGCGCACCGC
CGCCAAGTGCGCCGAACTGCGCGCACGCGGGGTCTGGTCCGAGGCCCTGCGCGAGCGCACCGGGCTGGAGCTGGACCCGT
ATTTCTCCGCCACCAAGCTTGCGTGGCTGCTCGATCATGTGCCTGACGCGCGGCGGCGCGCCGAGGCCGGCGAGCTGGCC
TTCGGCACGGTCGACAGCTGGCTGGTGTGGCACCTCAGCGGGGGCCGGCTGCACGTCACCGACCACGGCAACGCCGCCCG
CACGCTGCTGCTCGACATCCACCGGCGCGACTGGGACGACGCCTTGCTCGCGCTGTTCGACATCCCGCGCGCGGTCCTGC
CGCGTCTGGTCGACAGCATCGGCGTCTGCGGCGAGAGCGATCCGGCCGTGCTCGGCGCCGCAGTGCCGATTGCCGGCATC
GCCGGCGACCAGCAGGCGGCGACCTTCGGCCAGCACTGCTTTGCGCCCGGCATGGCCAAGAACACCTACGGCACCGGCTG
CTTCCTGCTGATGAACACGGGAGCGACGCCGGTGAAGTCCAGCCACCGCCTGCTTTCCACTGTTGGCTGGACGCAGGGCG
CCGCGCACACCTATGCGCTGGAGGGCAGCATCTTCATGGGCGGGGCAACCGTGCAGTGGCTGCGGGACGGACTTGGCCTC
ATCCGCGAAGCGGCCGAGGTGGAGGCGCTGGCGGCGCGGGTTCCCGACAGCGGCGGCGTGGTGCTGGTGCCGGCTTTCAC
CGGGCTGGGGGCACCGTACTGGGATGCGGCGGCACGCGGCGCGCTGTTCGGCCTTACCCGCGGCAGCGGCCCCGCCCACA
TTGCACGCGCCGCGCTGGACGCGATTGCGCTGCAGACCGACGACCTGGTCCGCGCGATGACGGCCGATGGCGCCGGCCCC
TTGCACGAGCTGCGGGTCGATGGCGGCGCCGCCGCCAACAACCTGTTGATGCAGTTGCAGGCCGACATGCTGGGCGTTGC
GGTCGTGCGGCCGCGCATGCTCGAGACCACCGCGCTCGGCGCGGCCTGTCTGGCCGGCATCGGTGCCGGCGTATGGCGGG
ACACCGACGCGCTCGCGCCACACTGGCAGATCGACCGCGTCTTCACGCCGGCATGGAGCGAGGACCAGCGTGGGGCAGTG
CGCGCACGCTGGGCAGACGCAGTCGCGCGTTCCCGGGCCTGGGCTGCATGA

Upstream 100 bases:

>100_bases
GGTGCGGCAGCGAATCCGGACGATGCGGACGGCGTGGGGATGACAGCCGGGCGCGGCCGAGGCTAGGCTAGGCGGAACAC
CGTATGCAGGGAGGCAGGAG

Downstream 100 bases:

>100_bases
GCGGGCCACCCGGGCAGTCCCGCGCCGCCTTGCTGGCGCGGCTGCGCGCAGACCCCGTCTGGGACGTGCTCGTGGTCGGC
GGCGGCGCAAGCGGCTTGGG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSYVLALDQGTTSSRAMVFDAAGRVRSVARRDFTQSFPQPGWVEHDPAEILRSQFDCARDALADAGLRAGQLAAIGIANQ
RETTVLWERASGRALAPAIVWQDRRTAAKCAELRARGVWSEALRERTGLELDPYFSATKLAWLLDHVPDARRRAEAGELA
FGTVDSWLVWHLSGGRLHVTDHGNAARTLLLDIHRRDWDDALLALFDIPRAVLPRLVDSIGVCGESDPAVLGAAVPIAGI
AGDQQAATFGQHCFAPGMAKNTYGTGCFLLMNTGATPVKSSHRLLSTVGWTQGAAHTYALEGSIFMGGATVQWLRDGLGL
IREAAEVEALAARVPDSGGVVLVPAFTGLGAPYWDAAARGALFGLTRGSGPAHIARAALDAIALQTDDLVRAMTADGAGP
LHELRVDGGAAANNLLMQLQADMLGVAVVRPRMLETTALGAACLAGIGAGVWRDTDALAPHWQIDRVFTPAWSEDQRGAV
RARWADAVARSRAWAA

Sequences:

>Translated_496_residues
MSYVLALDQGTTSSRAMVFDAAGRVRSVARRDFTQSFPQPGWVEHDPAEILRSQFDCARDALADAGLRAGQLAAIGIANQ
RETTVLWERASGRALAPAIVWQDRRTAAKCAELRARGVWSEALRERTGLELDPYFSATKLAWLLDHVPDARRRAEAGELA
FGTVDSWLVWHLSGGRLHVTDHGNAARTLLLDIHRRDWDDALLALFDIPRAVLPRLVDSIGVCGESDPAVLGAAVPIAGI
AGDQQAATFGQHCFAPGMAKNTYGTGCFLLMNTGATPVKSSHRLLSTVGWTQGAAHTYALEGSIFMGGATVQWLRDGLGL
IREAAEVEALAARVPDSGGVVLVPAFTGLGAPYWDAAARGALFGLTRGSGPAHIARAALDAIALQTDDLVRAMTADGAGP
LHELRVDGGAAANNLLMQLQADMLGVAVVRPRMLETTALGAACLAGIGAGVWRDTDALAPHWQIDRVFTPAWSEDQRGAV
RARWADAVARSRAWAA
>Mature_495_residues
SYVLALDQGTTSSRAMVFDAAGRVRSVARRDFTQSFPQPGWVEHDPAEILRSQFDCARDALADAGLRAGQLAAIGIANQR
ETTVLWERASGRALAPAIVWQDRRTAAKCAELRARGVWSEALRERTGLELDPYFSATKLAWLLDHVPDARRRAEAGELAF
GTVDSWLVWHLSGGRLHVTDHGNAARTLLLDIHRRDWDDALLALFDIPRAVLPRLVDSIGVCGESDPAVLGAAVPIAGIA
GDQQAATFGQHCFAPGMAKNTYGTGCFLLMNTGATPVKSSHRLLSTVGWTQGAAHTYALEGSIFMGGATVQWLRDGLGLI
REAAEVEALAARVPDSGGVVLVPAFTGLGAPYWDAAARGALFGLTRGSGPAHIARAALDAIALQTDDLVRAMTADGAGPL
HELRVDGGAAANNLLMQLQADMLGVAVVRPRMLETTALGAACLAGIGAGVWRDTDALAPHWQIDRVFTPAWSEDQRGAVR
ARWADAVARSRAWAA

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=503, Percent_Identity=43.3399602385686, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI4504007, Length=503, Percent_Identity=43.3399602385686, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI41393575, Length=503, Percent_Identity=43.3399602385686, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI42794763, Length=508, Percent_Identity=43.3070866141732, Blast_Score=394, Evalue=1e-110,
Organism=Homo sapiens, GI88196792, Length=513, Percent_Identity=31.5789473684211, Blast_Score=285, Evalue=5e-77,
Organism=Escherichia coli, GI1790361, Length=494, Percent_Identity=53.4412955465587, Blast_Score=543, Evalue=1e-155,
Organism=Escherichia coli, GI1789987, Length=474, Percent_Identity=27.6371308016878, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1787789, Length=485, Percent_Identity=27.4226804123711, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790005, Length=473, Percent_Identity=25.7928118393235, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789168, Length=488, Percent_Identity=26.6393442622951, Blast_Score=94, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17535599, Length=505, Percent_Identity=42.3762376237624, Blast_Score=384, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71986140, Length=519, Percent_Identity=25.4335260115607, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI71986147, Length=270, Percent_Identity=30.7407407407407, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321755, Length=555, Percent_Identity=35.1351351351351, Blast_Score=294, Evalue=2e-80,
Organism=Drosophila melanogaster, GI17864214, Length=507, Percent_Identity=41.025641025641, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24654573, Length=459, Percent_Identity=41.8300653594771, Blast_Score=352, Evalue=4e-97,
Organism=Drosophila melanogaster, GI24655555, Length=505, Percent_Identity=38.019801980198, Blast_Score=352, Evalue=5e-97,
Organism=Drosophila melanogaster, GI24655560, Length=505, Percent_Identity=38.019801980198, Blast_Score=352, Evalue=5e-97,
Organism=Drosophila melanogaster, GI24655570, Length=505, Percent_Identity=38.019801980198, Blast_Score=352, Evalue=5e-97,
Organism=Drosophila melanogaster, GI24655574, Length=505, Percent_Identity=38.019801980198, Blast_Score=352, Evalue=5e-97,
Organism=Drosophila melanogaster, GI24655565, Length=505, Percent_Identity=38.019801980198, Blast_Score=352, Evalue=5e-97,
Organism=Drosophila melanogaster, GI19922058, Length=526, Percent_Identity=31.7490494296578, Blast_Score=250, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24652871, Length=492, Percent_Identity=32.520325203252, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24656367, Length=516, Percent_Identity=29.8449612403101, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI21357867, Length=528, Percent_Identity=28.9772727272727, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI24656372, Length=349, Percent_Identity=32.0916905444126, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI45552909, Length=287, Percent_Identity=29.9651567944251, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_AZOSB (A1K962)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_934254.1
- ProteinModelPortal:   A1K962
- SMR:   A1K962
- STRING:   A1K962
- GeneID:   4608911
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo2751
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   GAPWWKP
- PhylomeDB:   A1K962
- ProtClustDB:   CLSK766828
- BioCyc:   ASP62928:AZO2751-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 52821; Mature: 52690

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 11-11 BINDING 15-15 BINDING 81-81 BINDING 134-134 BINDING 243-243 BINDING 265-265 BINDING 308-308

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYVLALDQGTTSSRAMVFDAAGRVRSVARRDFTQSFPQPGWVEHDPAEILRSQFDCARD
CCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
ALADAGLRAGQLAAIGIANQRETTVLWERASGRALAPAIVWQDRRTAAKCAELRARGVWS
HHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCEECCCEEECCCHHHHHHHHHHHCCCHH
EALRERTGLELDPYFSATKLAWLLDHVPDARRRAEAGELAFGTVDSWLVWHLSGGRLHVT
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCEEECCCCCEEEEEECCCEEEEE
DHGNAARTLLLDIHRRDWDDALLALFDIPRAVLPRLVDSIGVCGESDPAVLGAAVPIAGI
CCCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCC
AGDQQAATFGQHCFAPGMAKNTYGTGCFLLMNTGATPVKSSHRLLSTVGWTQGAAHTYAL
CCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCEEEEE
EGSIFMGGATVQWLRDGLGLIREAAEVEALAARVPDSGGVVLVPAFTGLGAPYWDAAARG
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHCC
ALFGLTRGSGPAHIARAALDAIALQTDDLVRAMTADGAGPLHELRVDGGAAANNLLMQLQ
CEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHEEEECCCCHHHHHHHHHH
ADMLGVAVVRPRMLETTALGAACLAGIGAGVWRDTDALAPHWQIDRVFTPAWSEDQRGAV
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECEEECCCCCCCCCCCH
RARWADAVARSRAWAA
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SYVLALDQGTTSSRAMVFDAAGRVRSVARRDFTQSFPQPGWVEHDPAEILRSQFDCARD
CEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
ALADAGLRAGQLAAIGIANQRETTVLWERASGRALAPAIVWQDRRTAAKCAELRARGVWS
HHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCEECCCEEECCCHHHHHHHHHHHCCCHH
EALRERTGLELDPYFSATKLAWLLDHVPDARRRAEAGELAFGTVDSWLVWHLSGGRLHVT
HHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCEEECCCCCEEEEEECCCEEEEE
DHGNAARTLLLDIHRRDWDDALLALFDIPRAVLPRLVDSIGVCGESDPAVLGAAVPIAGI
CCCCCCEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCC
AGDQQAATFGQHCFAPGMAKNTYGTGCFLLMNTGATPVKSSHRLLSTVGWTQGAAHTYAL
CCCCHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCEEEEE
EGSIFMGGATVQWLRDGLGLIREAAEVEALAARVPDSGGVVLVPAFTGLGAPYWDAAARG
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHCC
ALFGLTRGSGPAHIARAALDAIALQTDDLVRAMTADGAGPLHELRVDGGAAANNLLMQLQ
CEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHEEEECCCCHHHHHHHHHH
ADMLGVAVVRPRMLETTALGAACLAGIGAGVWRDTDALAPHWQIDRVFTPAWSEDQRGAV
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECEEECCCCCCCCCCCH
RARWADAVARSRAWAA
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA