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The map label for this gene is narX [C]

Identifier: 119899038

GI number: 119899038

Start: 3029233

End: 3031296

Strand: Direct

Name: narX [C]

Synonym: azo2748

Alternate gene names: 119899038

Gene position: 3029233-3031296 (Clockwise)

Preceding gene: 119899036

Following gene: 119899039

Centisome position: 69.22

GC content: 67.68

Gene sequence:

>2064_bases
ATGGGCGAACACCCGACAGGAAAGACGGCGTCCGCCCCCAGCGATGACGCCAGCGGCGGCTTGCAGACACAACTGGCTGC
CTGTTACGACGCGCTGCGCGAGCACGAGGACGCCTTGCAGCGCGCGCGCCAGCAACACGCCGAAAGCGAGGCGCGCTTCG
CCATGCTGGCGGACGCGGCGCCGGTGATGATCTGGATGACCGGGGCGGACTCGGAGTGCGTCTTCGTCAACCAGGCCTGG
CAACGTTTCACGGGCCGCAGTGCGGACGAAGAGCGCGGCGGCAACTGGACGCAGAACCTGCATCCGGACGACACCGCGCG
CTGCATCGCGGCCCACACAGCCGCCTTCGAGGCTCGCACCCCGTTCAGCATCGAATACCGCCTGCGCAGGGTGGACGGCG
TGTACCGCTGGCTGCTGGATTCGGCCCAGCCGCGCTATGACGGCAAGGGCCGCTTCCTGGGCTTCGTCGGCTGCTGCGTC
GACATCACCGAGCGCCGCGAAGCCGAGGACGGACTGAGACTGAAAGCCCGCTACCAGCGCGCGCTGCTCGACAACTTTCC
GTTTCTCGTCTGGCTGAAGGACACCGAAAGCCGCTTCCTGGCGGTCAATGCCGCCTTCACGCAAGCATTCGACATTGCCA
GCCCGGACGACCTCATCAACGGCACCGACTTCGACATCGTGCCCCCGGCGCTCGCTGAAAGTTACCGCGCCGGCGACCGC
AAGGTGCTGCGCGACGGCCATCAGCTGAGCATCGAAGAGGAGATTCCGTACCACGGTGGCCGCCGCTGGTTCGAGACCTA
CAAGGCGCCGGTGTTCGACGACGACGGCCGCATCGTCGGCACCGTGGGCTTCGCACGCGACGTGACCGAGCGCAAGGACG
CCGAAGGGGCGTTGCTGCGCTTGAAGGACACGCTGGAGGAACAGGTGGCGCTGCGCACCGCGGAAGCCGAAGCGCGCGCC
CGCGCGCTGTGGGAATCCGAACGTTTCTCGCGCGCCACCATCGACGCCCTGCCCTGCGCCCTGTGCGTGCTGGACGGCAG
CGGCGTCATCGTGGCGGTCAACAAGTCGTGGCGGGCGTTGGTGATCACCAACGGCGGCGACATCGAAACCATGTGCGAAG
GCGCCAGCTACCTCGCCGCCTGCCAGCCCGCCACGCGGATGAACGATGCCGCGCTGACGAACGCGACCATGGCGGTGCGC
GGCATCCTCACCGGAGAACTCGACAGCTACGCATTCGAGTACGAAGGCCTGCTCGGCGAGGACCGGCGCTGGTTCAGCTT
CGAAGTCAGCCCCTTCCCCGACCACGGGCCGGTCCGCCTGCTGGTCAAGCACGACGACATGACCGAGCGCCGCCACCTGC
AGGAAGAGCAGGTGGAAACCGCGGCGCGCTTCAAGCGTCTGGCCGCCCACCTCGACATGGTGCGCGAGGAGCAAAGCACC
AAGATCGCCCGCGAAGTGCATGACGAACTCGGCAGCACGCTGACGATGCTCAAGCTTGGCCTCGCCACGGTGGCCGACGA
CGAAACCACGCCCCCCAGGATCCGCAACAAGTTCGCCGGCATGCTGGAGCAGATCGATGCCGCGCTGCAGACGGTGAAGC
GCATCTCGACCAGCCTGCGTCCGGCCACGCTGGACACGCTCGGCCTGATCGCGACCATCAGCTGGTACGCCCGCCAGTTC
TCCAGCATGACCGGCATCGACGTCGCGCTGCAGATGCCCGAATACGTGCGGCTGACGCCGGCCGCCAACACCGCGGTGTT
CCGCATCATCCAGGAAGGCCTCACCAACGTCGCCAAGCACGCCGGCGCCAACCGGGTGGACCTGACCCTGCAGAAGCAGT
CGGGACAGCTCGTCGTCCGCATCAGCGACAACGGCGTCGGCCTGACCTCCGACAGCCTCAGCAAGCGCAATTCCTTCGGC
GTCATCGGCATGCACGAACGCGCCCAGCACCTCGGCGGGCGCCTCACCCTGAGCGGCGCGCCGCAAACCGGCACCCGGCT
CACGCTGACCATTCCGCTGGACGCGGGCGCCGCGCACAGCCCGGAGAAAACCCCATCATGGTGA

Upstream 100 bases:

>100_bases
GCGCAAGTGGTGTAAATATGCGGACCTGCGGTTGCGCGTTCGCGCGCGCGTCTGCGCCCGAAGTCACGGGCGCCCGACTC
AGCGACAGGAGAACATTGGC

Downstream 100 bases:

>100_bases
AAGTGCTCATTGCCGACGATCACGCCGTCGTGCGCGGCGGGCTGCGCCAGTTCCTGTCCTCTACCGAAGAGTTCGACATC
GTCGCCGAGGCCGCCAACGG

Product: putative sensory box histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MGEHPTGKTASAPSDDASGGLQTQLAACYDALREHEDALQRARQQHAESEARFAMLADAAPVMIWMTGADSECVFVNQAW
QRFTGRSADEERGGNWTQNLHPDDTARCIAAHTAAFEARTPFSIEYRLRRVDGVYRWLLDSAQPRYDGKGRFLGFVGCCV
DITERREAEDGLRLKARYQRALLDNFPFLVWLKDTESRFLAVNAAFTQAFDIASPDDLINGTDFDIVPPALAESYRAGDR
KVLRDGHQLSIEEEIPYHGGRRWFETYKAPVFDDDGRIVGTVGFARDVTERKDAEGALLRLKDTLEEQVALRTAEAEARA
RALWESERFSRATIDALPCALCVLDGSGVIVAVNKSWRALVITNGGDIETMCEGASYLAACQPATRMNDAALTNATMAVR
GILTGELDSYAFEYEGLLGEDRRWFSFEVSPFPDHGPVRLLVKHDDMTERRHLQEEQVETAARFKRLAAHLDMVREEQST
KIAREVHDELGSTLTMLKLGLATVADDETTPPRIRNKFAGMLEQIDAALQTVKRISTSLRPATLDTLGLIATISWYARQF
SSMTGIDVALQMPEYVRLTPAANTAVFRIIQEGLTNVAKHAGANRVDLTLQKQSGQLVVRISDNGVGLTSDSLSKRNSFG
VIGMHERAQHLGGRLTLSGAPQTGTRLTLTIPLDAGAAHSPEKTPSW

Sequences:

>Translated_687_residues
MGEHPTGKTASAPSDDASGGLQTQLAACYDALREHEDALQRARQQHAESEARFAMLADAAPVMIWMTGADSECVFVNQAW
QRFTGRSADEERGGNWTQNLHPDDTARCIAAHTAAFEARTPFSIEYRLRRVDGVYRWLLDSAQPRYDGKGRFLGFVGCCV
DITERREAEDGLRLKARYQRALLDNFPFLVWLKDTESRFLAVNAAFTQAFDIASPDDLINGTDFDIVPPALAESYRAGDR
KVLRDGHQLSIEEEIPYHGGRRWFETYKAPVFDDDGRIVGTVGFARDVTERKDAEGALLRLKDTLEEQVALRTAEAEARA
RALWESERFSRATIDALPCALCVLDGSGVIVAVNKSWRALVITNGGDIETMCEGASYLAACQPATRMNDAALTNATMAVR
GILTGELDSYAFEYEGLLGEDRRWFSFEVSPFPDHGPVRLLVKHDDMTERRHLQEEQVETAARFKRLAAHLDMVREEQST
KIAREVHDELGSTLTMLKLGLATVADDETTPPRIRNKFAGMLEQIDAALQTVKRISTSLRPATLDTLGLIATISWYARQF
SSMTGIDVALQMPEYVRLTPAANTAVFRIIQEGLTNVAKHAGANRVDLTLQKQSGQLVVRISDNGVGLTSDSLSKRNSFG
VIGMHERAQHLGGRLTLSGAPQTGTRLTLTIPLDAGAAHSPEKTPSW
>Mature_686_residues
GEHPTGKTASAPSDDASGGLQTQLAACYDALREHEDALQRARQQHAESEARFAMLADAAPVMIWMTGADSECVFVNQAWQ
RFTGRSADEERGGNWTQNLHPDDTARCIAAHTAAFEARTPFSIEYRLRRVDGVYRWLLDSAQPRYDGKGRFLGFVGCCVD
ITERREAEDGLRLKARYQRALLDNFPFLVWLKDTESRFLAVNAAFTQAFDIASPDDLINGTDFDIVPPALAESYRAGDRK
VLRDGHQLSIEEEIPYHGGRRWFETYKAPVFDDDGRIVGTVGFARDVTERKDAEGALLRLKDTLEEQVALRTAEAEARAR
ALWESERFSRATIDALPCALCVLDGSGVIVAVNKSWRALVITNGGDIETMCEGASYLAACQPATRMNDAALTNATMAVRG
ILTGELDSYAFEYEGLLGEDRRWFSFEVSPFPDHGPVRLLVKHDDMTERRHLQEEQVETAARFKRLAAHLDMVREEQSTK
IAREVHDELGSTLTMLKLGLATVADDETTPPRIRNKFAGMLEQIDAALQTVKRISTSLRPATLDTLGLIATISWYARQFS
SMTGIDVALQMPEYVRLTPAANTAVFRIIQEGLTNVAKHAGANRVDLTLQKQSGQLVVRISDNGVGLTSDSLSKRNSFGV
IGMHERAQHLGGRLTLSGAPQTGTRLTLTIPLDAGAAHSPEKTPSW

Specific function: Acts As A Sensor For Nitrate/Nitrite And Transduces Signal Of Nitrate Availability To The Narl Protein And Of Both Nitrate/Nitrite To The Narp Protein. Narx Probably Activates Narl And Narp By Phosphorylation In The Presence Of Nitrate. Narx Also Plays A

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=214, Percent_Identity=26.6355140186916, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082311, Length=245, Percent_Identity=26.1224489795918, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI48994928, Length=147, Percent_Identity=31.9727891156463, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013656
- InterPro:   IPR013655 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF08447 PAS_3; PF08448 PAS_4 [H]

EC number: 2.7.3.- [C]

Molecular weight: Translated: 75944; Mature: 75813

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEHPTGKTASAPSDDASGGLQTQLAACYDALREHEDALQRARQQHAESEARFAMLADAA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
PVMIWMTGADSECVFVNQAWQRFTGRSADEERGGNWTQNLHPDDTARCIAAHTAAFEART
CEEEEEECCCCCEEEHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
PFSIEYRLRRVDGVYRWLLDSAQPRYDGKGRFLGFVGCCVDITERREAEDGLRLKARYQR
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHH
ALLDNFPFLVWLKDTESRFLAVNAAFTQAFDIASPDDLINGTDFDIVPPALAESYRAGDR
HHHHCCCEEEEEECCCCEEEEEEEHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCH
KVLRDGHQLSIEEEIPYHGGRRWFETYKAPVFDDDGRIVGTVGFARDVTERKDAEGALLR
HHHCCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHCCCCCCEEH
LKDTLEEQVALRTAEAEARARALWESERFSRATIDALPCALCVLDGSGVIVAVNKSWRAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEEEECCCCEEE
VITNGGDIETMCEGASYLAACQPATRMNDAALTNATMAVRGILTGELDSYAFEYEGLLGE
EEECCCCHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHEEHCCCHHHHHEEECCCCCC
DRRWFSFEVSPFPDHGPVRLLVKHDDMTERRHLQEEQVETAARFKRLAAHLDMVREEQST
CCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIAREVHDELGSTLTMLKLGLATVADDETTPPRIRNKFAGMLEQIDAALQTVKRISTSLR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PATLDTLGLIATISWYARQFSSMTGIDVALQMPEYVRLTPAANTAVFRIIQEGLTNVAKH
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHH
AGANRVDLTLQKQSGQLVVRISDNGVGLTSDSLSKRNSFGVIGMHERAQHLGGRLTLSGA
CCCCEEEEEEEECCCCEEEEECCCCCCCCCHHHCCCCCCCEEECHHHHHHCCCEEEECCC
PQTGTRLTLTIPLDAGAAHSPEKTPSW
CCCCCEEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
GEHPTGKTASAPSDDASGGLQTQLAACYDALREHEDALQRARQQHAESEARFAMLADAA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
PVMIWMTGADSECVFVNQAWQRFTGRSADEERGGNWTQNLHPDDTARCIAAHTAAFEART
CEEEEEECCCCCEEEHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
PFSIEYRLRRVDGVYRWLLDSAQPRYDGKGRFLGFVGCCVDITERREAEDGLRLKARYQR
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCHHHHHHHH
ALLDNFPFLVWLKDTESRFLAVNAAFTQAFDIASPDDLINGTDFDIVPPALAESYRAGDR
HHHHCCCEEEEEECCCCEEEEEEEHHHHHCCCCCCHHHCCCCCCCCCCHHHHHHHCCCCH
KVLRDGHQLSIEEEIPYHGGRRWFETYKAPVFDDDGRIVGTVGFARDVTERKDAEGALLR
HHHCCCCEEEECCCCCCCCCHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHCCCCCCEEH
LKDTLEEQVALRTAEAEARARALWESERFSRATIDALPCALCVLDGSGVIVAVNKSWRAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEEEECCCCEEE
VITNGGDIETMCEGASYLAACQPATRMNDAALTNATMAVRGILTGELDSYAFEYEGLLGE
EEECCCCHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHEEHCCCHHHHHEEECCCCCC
DRRWFSFEVSPFPDHGPVRLLVKHDDMTERRHLQEEQVETAARFKRLAAHLDMVREEQST
CCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIAREVHDELGSTLTMLKLGLATVADDETTPPRIRNKFAGMLEQIDAALQTVKRISTSLR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PATLDTLGLIATISWYARQFSSMTGIDVALQMPEYVRLTPAANTAVFRIIQEGLTNVAKH
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHH
AGANRVDLTLQKQSGQLVVRISDNGVGLTSDSLSKRNSFGVIGMHERAQHLGGRLTLSGA
CCCCEEEEEEEECCCCEEEEECCCCCCCCCHHHCCCCCCCEEECHHHHHHCCCEEEECCC
PQTGTRLTLTIPLDAGAAHSPEKTPSW
CCCCCEEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with a nitrogenous group as acceptor [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]