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The map label for this gene is rffA [H]

Identifier: 119898966

GI number: 119898966

Start: 2950840

End: 2951982

Strand: Reverse

Name: rffA [H]

Synonym: azo2675

Alternate gene names: 119898966

Gene position: 2951982-2950840 (Counterclockwise)

Preceding gene: 119898967

Following gene: 119898965

Centisome position: 67.46

GC content: 52.93

Gene sequence:

>1143_bases
ATGCTGGAAGAGAAGAAGATCCCGTTCAACTGGCCTTACATGACAGGCAAGGAGTTGTACTACATCGCTGAAGCACACTT
TAATGGCAGGTTGGCAGGGGATGGGCCTTTCACAAAGCGCTGCCATCGCTGGCTGGAGGAGAGGACTGGATGCAATAAGG
GCTTGCTTACGCACTCGTGCACGGCAGCACTCGAGATGGCTGCTTTATTGCTGGATATTCAGCCTGGTGATGAGATCATC
ATGCCGTCATACACCTTTGTATCTACCGCCAATGCCTTTGTGCTTCGTGGTGGAGTCCCAGTGTTTGTCGATATCAGAGA
AGATTCGCTGAATTTGGACGAACGTCTGATCGAGTCTGCCATAACGCCGCGTACCCGTGCCATTGCTCCGGTTCATTACG
CGGGAGTCGCGTGCGAGATGGATACGATCATGATCATGGCCCGAGAGCATGGATTGAAAGTCGTTGAGGATGCGGCGCAG
GGGGTGATGTCGACCTACAAGGGGCGGCCTCTGGGTAGCATTGGTGATCTCGGAGTTTACAGTTTTCACGAAACAAAGAA
CCTGATCTCTGGAGAAGGCGGCGCGCTGCTCGTCAATAGTCCCGACATGAGCCTTCGCGCAGAGATCATTCGCGAGAAAG
GGACTGACCGAAGCCGATTCTTCAGGGGAGAAGTCGATAAGTACACTTGGCAAGAGGTGGGCTCGTCTTTCCTGCCAGGG
GAGTTGATTGCCGCTTTCCTGTGGGCGCAACTTGAAGAAGCGGATCGAATTACTAAAGAGCGGTTGGCTAGCTGGCAACG
TTATCACGAGTTTCTGGAGCCGCTTGAAGCCAAAGGAATACTGAGGCGCCCGATTGTTCCAGGTGACTGTCAGCACAATG
CTCACATGTACTACGTTCTCCTGGCGCCTGAAATTAATCGTCAAGAGGTCCTTGATTCGTTCAAACGAAATGAAATCTGG
TCGGTTTTCCATTACGTACCGCTTCACTCATCCCCTGCCGGTAAGCGTTATGGTCGGTCGCATGGCGACCTGGATGTCAC
AACTCGGCTGTCTGAAAGGTTGGTCCGCTTGCCGATGTGGGTTGGGTTGACGAATGAGCAGCAAGAAAGAGTCGTTGAGG
TGCTGAGTGAAGCGGTTAGCTGA

Upstream 100 bases:

>100_bases
TTCCAGACTACTTGTCGCCCGGAAAAAGAGTTGATGTTACTACTTGGAATAATGGCGTGATGTCTGGTTTGCCAGACTAA
ATTTTCGTGCGAAGGTTTAA

Downstream 100 bases:

>100_bases
GCTTTACAATCCTAAAATTAAGGATTCTGTTGTGGCTCAACCAGAAACTAATCGTATGCATTTGCTGGGTGAAGGTTCGC
ATATGCCGCCAATTTTCGCT

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MLEEKKIPFNWPYMTGKELYYIAEAHFNGRLAGDGPFTKRCHRWLEERTGCNKGLLTHSCTAALEMAALLLDIQPGDEII
MPSYTFVSTANAFVLRGGVPVFVDIREDSLNLDERLIESAITPRTRAIAPVHYAGVACEMDTIMIMAREHGLKVVEDAAQ
GVMSTYKGRPLGSIGDLGVYSFHETKNLISGEGGALLVNSPDMSLRAEIIREKGTDRSRFFRGEVDKYTWQEVGSSFLPG
ELIAAFLWAQLEEADRITKERLASWQRYHEFLEPLEAKGILRRPIVPGDCQHNAHMYYVLLAPEINRQEVLDSFKRNEIW
SVFHYVPLHSSPAGKRYGRSHGDLDVTTRLSERLVRLPMWVGLTNEQQERVVEVLSEAVS

Sequences:

>Translated_380_residues
MLEEKKIPFNWPYMTGKELYYIAEAHFNGRLAGDGPFTKRCHRWLEERTGCNKGLLTHSCTAALEMAALLLDIQPGDEII
MPSYTFVSTANAFVLRGGVPVFVDIREDSLNLDERLIESAITPRTRAIAPVHYAGVACEMDTIMIMAREHGLKVVEDAAQ
GVMSTYKGRPLGSIGDLGVYSFHETKNLISGEGGALLVNSPDMSLRAEIIREKGTDRSRFFRGEVDKYTWQEVGSSFLPG
ELIAAFLWAQLEEADRITKERLASWQRYHEFLEPLEAKGILRRPIVPGDCQHNAHMYYVLLAPEINRQEVLDSFKRNEIW
SVFHYVPLHSSPAGKRYGRSHGDLDVTTRLSERLVRLPMWVGLTNEQQERVVEVLSEAVS
>Mature_380_residues
MLEEKKIPFNWPYMTGKELYYIAEAHFNGRLAGDGPFTKRCHRWLEERTGCNKGLLTHSCTAALEMAALLLDIQPGDEII
MPSYTFVSTANAFVLRGGVPVFVDIREDSLNLDERLIESAITPRTRAIAPVHYAGVACEMDTIMIMAREHGLKVVEDAAQ
GVMSTYKGRPLGSIGDLGVYSFHETKNLISGEGGALLVNSPDMSLRAEIIREKGTDRSRFFRGEVDKYTWQEVGSSFLPG
ELIAAFLWAQLEEADRITKERLASWQRYHEFLEPLEAKGILRRPIVPGDCQHNAHMYYVLLAPEINRQEVLDSFKRNEIW
SVFHYVPLHSSPAGKRYGRSHGDLDVTTRLSERLVRLPMWVGLTNEQQERVVEVLSEAVS

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=370, Percent_Identity=60.5405405405405, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI145693159, Length=387, Percent_Identity=28.6821705426357, Blast_Score=155, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 42970; Mature: 42970

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEEKKIPFNWPYMTGKELYYIAEAHFNGRLAGDGPFTKRCHRWLEERTGCNKGLLTHSC
CCCCCCCCCCCCCCCCCEEEEEEEECCCCEECCCCCHHHHHHHHHHHHCCCCCCCHHHHH
TAALEMAALLLDIQPGDEIIMPSYTFVSTANAFVLRGGVPVFVDIREDSLNLDERLIESA
HHHHHHHHHHEECCCCCCEECCCCHHHHCCCEEEEECCCEEEEEECCCCCCHHHHHHHHH
ITPRTRAIAPVHYAGVACEMDTIMIMAREHGLKVVEDAAQGVMSTYKGRPLGSIGDLGVY
CCCCHHEECCCHHCCEEEEHHHEEEEEHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCE
SFHETKNLISGEGGALLVNSPDMSLRAEIIREKGTDRSRFFRGEVDKYTWQEVGSSFLPG
EHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHCCCCCCH
ELIAAFLWAQLEEADRITKERLASWQRYHEFLEPLEAKGILRRPIVPGDCQHNAHMYYVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEEEE
LAPEINRQEVLDSFKRNEIWSVFHYVPLHSSPAGKRYGRSHGDLDVTTRLSERLVRLPMW
ECCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHE
VGLTNEQQERVVEVLSEAVS
ECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLEEKKIPFNWPYMTGKELYYIAEAHFNGRLAGDGPFTKRCHRWLEERTGCNKGLLTHSC
CCCCCCCCCCCCCCCCCEEEEEEEECCCCEECCCCCHHHHHHHHHHHHCCCCCCCHHHHH
TAALEMAALLLDIQPGDEIIMPSYTFVSTANAFVLRGGVPVFVDIREDSLNLDERLIESA
HHHHHHHHHHEECCCCCCEECCCCHHHHCCCEEEEECCCEEEEEECCCCCCHHHHHHHHH
ITPRTRAIAPVHYAGVACEMDTIMIMAREHGLKVVEDAAQGVMSTYKGRPLGSIGDLGVY
CCCCHHEECCCHHCCEEEEHHHEEEEEHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCE
SFHETKNLISGEGGALLVNSPDMSLRAEIIREKGTDRSRFFRGEVDKYTWQEVGSSFLPG
EHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHCCCCCCH
ELIAAFLWAQLEEADRITKERLASWQRYHEFLEPLEAKGILRRPIVPGDCQHNAHMYYVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEEEEE
LAPEINRQEVLDSFKRNEIWSVFHYVPLHSSPAGKRYGRSHGDLDVTTRLSERLVRLPMW
ECCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHE
VGLTNEQQERVVEVLSEAVS
ECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]