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The map label for this gene is nerA [H]

Identifier: 119898959

GI number: 119898959

Start: 2942246

End: 2943358

Strand: Direct

Name: nerA [H]

Synonym: azo2668

Alternate gene names: 119898959

Gene position: 2942246-2943358 (Clockwise)

Preceding gene: 119898958

Following gene: 119898961

Centisome position: 67.24

GC content: 69.63

Gene sequence:

>1113_bases
ATGACGACCCTGTTCGACCCGATCGCCGCCGGCGACCTCCAGCTCGCCAACCGAATCGCCATGGCGCCGCTCACCCGCAA
CCGTGCCCCGAATGCGGTGCCGGCCGCGATCACCGCCACCTACTACGCCCAGCGCGCCAGCGCGGGTCTGCTGATCAGCG
AGGCAACGGCGATCAGCCAGCAGGGCCAGGGTTATGCCGACGTGCCGGGCCTGTATGGCGCCGACCAGATCGCGGGCTGG
AAGAAGGTGACCGACGCGGTGCACGCGGCCGGCGGCAGGATCGTCGTCCAGCTGTGGCACGTGGGCCGCGTCTCGCACAC
CTCGCTGCAGCCTGGCGGCGCCGCGCCGGTGGCACCGTCGGCCATTGCCGCCCATACCAAGACCTACATCATCGAAGCCG
ACGGCAAGGGCAGCTTCGTGCCCACCTCCACCCCGCGCGCGCTGGATATCGAAGAGCTGCCCGGCATCGTCGAAGACTAC
CGGCGTGCAGCCCGCGCCGCGATCGAAGCGGGCTTCGACGGCGTCGAAGTGCACGCCGCCAACGGCTACCTGATCGACCA
GTTCCTGCGCGCCGGCAGCAACCAGCGCACCGACGCCTACGGCGGCAGCATCGAGAACCGCACCCGCCTGCTGGTGGAGG
TGATGGAAGCGATCACCGCCGAGATCGGCGCCAGCCGCACCGGCATCCGCATCTCGCCGGTCACCCCGGCCAATGACATC
GCCGACCCCGATCCGCAGCCGCTGTTCACCCACGTGGTGGAGCAACTCGCGCGCTGGCCGCTCGCCTACGTCCATGTCAT
CGAAGGCGCCACCGGGGGCGCGCGCGACTTCCAGCAGGGCGACCAGCCCTTCGACTACGCCGCGCTGCGCGCCGCCTACC
AGGCCGCCGGCGGCAAGGCCGCGTGGATGGTGAACAACGGCTACACCCGCGAACTGGCGGAAGCGGCGGTGGCAGAAGGC
CGCGCCGACATCGTCGCCTTCGGCAAGGCATTCATCTCCAACCCGGACCTCGTCGTGCGCCTTCGCGAGAAGGTTGCGCT
GAATCCGTGGGACACCACCACCTTCTACGGTGGCGGCGAAAAGGGCTATCTCGACTATCCCACGCTGGGCTGA

Upstream 100 bases:

>100_bases
AACGAAGACACCATCGCTGAATTCCTGCGCCAGATCGGCAGCGAGCTTTGACCGCGCACCGCCCCCTTCCCCTGCCCACG
TTACGGAGAACCCCCACACC

Downstream 100 bases:

>100_bases
GCGCCGCCCGGCCGGTGCCGGGCGGGATACGCTGAAGCGCCGTGCGGCGGCGGGCGGCGCCCGTCCGCCGCGCGCGCAGG
GCAGCAGCGTCAGCGCGCTG

Product: glycerol trinitrate reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTTLFDPIAAGDLQLANRIAMAPLTRNRAPNAVPAAITATYYAQRASAGLLISEATAISQQGQGYADVPGLYGADQIAGW
KKVTDAVHAAGGRIVVQLWHVGRVSHTSLQPGGAAPVAPSAIAAHTKTYIIEADGKGSFVPTSTPRALDIEELPGIVEDY
RRAARAAIEAGFDGVEVHAANGYLIDQFLRAGSNQRTDAYGGSIENRTRLLVEVMEAITAEIGASRTGIRISPVTPANDI
ADPDPQPLFTHVVEQLARWPLAYVHVIEGATGGARDFQQGDQPFDYAALRAAYQAAGGKAAWMVNNGYTRELAEAAVAEG
RADIVAFGKAFISNPDLVVRLREKVALNPWDTTTFYGGGEKGYLDYPTLG

Sequences:

>Translated_370_residues
MTTLFDPIAAGDLQLANRIAMAPLTRNRAPNAVPAAITATYYAQRASAGLLISEATAISQQGQGYADVPGLYGADQIAGW
KKVTDAVHAAGGRIVVQLWHVGRVSHTSLQPGGAAPVAPSAIAAHTKTYIIEADGKGSFVPTSTPRALDIEELPGIVEDY
RRAARAAIEAGFDGVEVHAANGYLIDQFLRAGSNQRTDAYGGSIENRTRLLVEVMEAITAEIGASRTGIRISPVTPANDI
ADPDPQPLFTHVVEQLARWPLAYVHVIEGATGGARDFQQGDQPFDYAALRAAYQAAGGKAAWMVNNGYTRELAEAAVAEG
RADIVAFGKAFISNPDLVVRLREKVALNPWDTTTFYGGGEKGYLDYPTLG
>Mature_369_residues
TTLFDPIAAGDLQLANRIAMAPLTRNRAPNAVPAAITATYYAQRASAGLLISEATAISQQGQGYADVPGLYGADQIAGWK
KVTDAVHAAGGRIVVQLWHVGRVSHTSLQPGGAAPVAPSAIAAHTKTYIIEADGKGSFVPTSTPRALDIEELPGIVEDYR
RAARAAIEAGFDGVEVHAANGYLIDQFLRAGSNQRTDAYGGSIENRTRLLVEVMEAITAEIGASRTGIRISPVTPANDIA
DPDPQPLFTHVVEQLARWPLAYVHVIEGATGGARDFQQGDQPFDYAALRAAYQAAGGKAAWMVNNGYTRELAEAAVAEGR
ADIVAFGKAFISNPDLVVRLREKVALNPWDTTTFYGGGEKGYLDYPTLG

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=369, Percent_Identity=49.5934959349593, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1789463, Length=223, Percent_Identity=35.4260089686099, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17559804, Length=159, Percent_Identity=38.3647798742138, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17559802, Length=148, Percent_Identity=38.5135135135135, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17565138, Length=152, Percent_Identity=38.1578947368421, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=168, Percent_Identity=35.7142857142857, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17564188, Length=153, Percent_Identity=35.2941176470588, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=157, Percent_Identity=34.3949044585987, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17566914, Length=77, Percent_Identity=41.5584415584416, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=386, Percent_Identity=36.0103626943005, Blast_Score=219, Evalue=7e-58,
Organism=Saccharomyces cerevisiae, GI6321973, Length=386, Percent_Identity=35.2331606217617, Blast_Score=202, Evalue=8e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39213; Mature: 39082

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLFDPIAAGDLQLANRIAMAPLTRNRAPNAVPAAITATYYAQRASAGLLISEATAISQ
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHHHHH
QGQGYADVPGLYGADQIAGWKKVTDAVHAAGGRIVVQLWHVGRVSHTSLQPGGAAPVAPS
CCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCH
AIAAHTKTYIIEADGKGSFVPTSTPRALDIEELPGIVEDYRRAARAAIEAGFDGVEVHAA
HHHHCCEEEEEEECCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEC
NGYLIDQFLRAGSNQRTDAYGGSIENRTRLLVEVMEAITAEIGASRTGIRISPVTPANDI
CCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
ADPDPQPLFTHVVEQLARWPLAYVHVIEGATGGARDFQQGDQPFDYAALRAAYQAAGGKA
CCCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCE
AWMVNNGYTRELAEAAVAEGRADIVAFGKAFISNPDLVVRLREKVALNPWDTTTFYGGGE
EEEECCCCHHHHHHHHHHCCCHHHEEHHHHHHCCCHHEEEEHHHHCCCCCCCCEEECCCC
KGYLDYPTLG
CCCCCCCCCC
>Mature Secondary Structure 
TTLFDPIAAGDLQLANRIAMAPLTRNRAPNAVPAAITATYYAQRASAGLLISEATAISQ
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHHHHH
QGQGYADVPGLYGADQIAGWKKVTDAVHAAGGRIVVQLWHVGRVSHTSLQPGGAAPVAPS
CCCCCCCCCCCCCCHHHCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCH
AIAAHTKTYIIEADGKGSFVPTSTPRALDIEELPGIVEDYRRAARAAIEAGFDGVEVHAA
HHHHCCEEEEEEECCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEC
NGYLIDQFLRAGSNQRTDAYGGSIENRTRLLVEVMEAITAEIGASRTGIRISPVTPANDI
CCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCC
ADPDPQPLFTHVVEQLARWPLAYVHVIEGATGGARDFQQGDQPFDYAALRAAYQAAGGKA
CCCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCE
AWMVNNGYTRELAEAAVAEGRADIVAFGKAFISNPDLVVRLREKVALNPWDTTTFYGGGE
EEEECCCCHHHHHHHHHHCCCHHHEEHHHHHHCCCHHEEEEHHHHCCCCCCCCEEECCCC
KGYLDYPTLG
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]