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The map label for this gene is pleD [H]

Identifier: 119898913

GI number: 119898913

Start: 2902117

End: 2903265

Strand: Reverse

Name: pleD [H]

Synonym: azo2622

Alternate gene names: 119898913

Gene position: 2903265-2902117 (Counterclockwise)

Preceding gene: 119898914

Following gene: 119898912

Centisome position: 66.34

GC content: 65.01

Gene sequence:

>1149_bases
ATGGCACTCGACATCCGCACGATTTTCTTCATGCTCGGCCTCGCGACGCTGGCCGCTGCCGTATCGCTCTTTTTGGCGCA
ACGGGTCCGGTTAGCCGTGCATGGCATCGAGTGCTGGGCCTGGGGCATGGCTTGCGCAGCCTGCAGTGGCTTTCTCCTCG
CCCAACGTGGTGCCCTCTCCCCCCTGCTCACTGTCGTCGCTGCCAACAGCGTCCTCCTTCTCGGGCTCAGCCTCTTCTGG
CTCGGCATGCGCCGCTTTGTCGGCCGCTCCTGCAGTCCCAGCTGGATATTCGCTGCACCCGCCATCGCCCTACCTTTCCT
CGCCACCCGCTATCTGGACGACAGCGACCCGGCAGGGCGCATCAGCTTCCTTTCCATCATCGTTGCCCTGCTGCTCTTCC
TCACCGCCAGCGAACTGCTCCGTCACGGCGAAAGGGGGCGTCTCGGCCGCCGCTGGGTCGCCCTGCTCTTGATCCTGCAC
GGCCTCTTCTTTTGTGTCCGCGCCATCCTCAGCGCCACCGGCACGCCTCTAAGCGACCCCTTCGGCAGCGGCCGCCTGGC
CATGATGACCGGCCTCGAAGGCTTTCTGTTCATCCTCTTTTTCACTCTTGGCTTGGTCATCATGACCAGCGAAACCCTGC
AGGCCGAACTCAACCGCCAGGCCACCCGTGATCCGCTTACCGACGTCTACAACCGTCGCGCCTTCCATGACCTATCGGCC
CACGAGCTGGCGCGCGCGCAGCGCTCGGGCCGCGCACTCGCGCTGCTTCTCATCGACCTCGACCACTTCAAGCAACTCAA
CGACCGGCATGGCCACCACGTCGGCGACCTTGCCTTGAAGCACTTTGTCGCCCGCGTCGCTCCCTGCCTGCGCCATCAGG
ACATCTTCGCCCGATACGGCGGTGAGGAATTCGTCGTCCTGCTGCCGGACACCGAGGTCACCACAGCCCTTGTGGTGGCC
GAACGCATTCGCGCCGCTGTCGCCGGCCACCCTCTAGAAACGAGCAGGGGGCCAGTGACCATCACGGTCAGCATCGGCGT
CGCCACGACCCTGCCGACTTCTCCCGACATCACCGCCACCATTAACAGTGCCGACGCCGCCCTCTATCGGGCCAAGGCGG
AGGGCCGTAACCGCGCTGTCAGCGGATGA

Upstream 100 bases:

>100_bases
AAGACGTGCTTGGCCAGGTCGACGGCAACGGTAGTAGCATCCATGACGGACTCCTCCTCCTGAAGACCGATCCCCATCCC
ATGACCCTAATTAGGTTTGC

Downstream 100 bases:

>100_bases
CTTCGACTGGTCGACATCCAATGGACTGGCGAACTGGCGCTCGCTACACGGCAGAAAGGAAACGTCGAATGCCCACCCAA
ATCCTCACCTTCTGGTTCTC

Product: hypothetical protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MALDIRTIFFMLGLATLAAAVSLFLAQRVRLAVHGIECWAWGMACAACSGFLLAQRGALSPLLTVVAANSVLLLGLSLFW
LGMRRFVGRSCSPSWIFAAPAIALPFLATRYLDDSDPAGRISFLSIIVALLLFLTASELLRHGERGRLGRRWVALLLILH
GLFFCVRAILSATGTPLSDPFGSGRLAMMTGLEGFLFILFFTLGLVIMTSETLQAELNRQATRDPLTDVYNRRAFHDLSA
HELARAQRSGRALALLLIDLDHFKQLNDRHGHHVGDLALKHFVARVAPCLRHQDIFARYGGEEFVVLLPDTEVTTALVVA
ERIRAAVAGHPLETSRGPVTITVSIGVATTLPTSPDITATINSADAALYRAKAEGRNRAVSG

Sequences:

>Translated_382_residues
MALDIRTIFFMLGLATLAAAVSLFLAQRVRLAVHGIECWAWGMACAACSGFLLAQRGALSPLLTVVAANSVLLLGLSLFW
LGMRRFVGRSCSPSWIFAAPAIALPFLATRYLDDSDPAGRISFLSIIVALLLFLTASELLRHGERGRLGRRWVALLLILH
GLFFCVRAILSATGTPLSDPFGSGRLAMMTGLEGFLFILFFTLGLVIMTSETLQAELNRQATRDPLTDVYNRRAFHDLSA
HELARAQRSGRALALLLIDLDHFKQLNDRHGHHVGDLALKHFVARVAPCLRHQDIFARYGGEEFVVLLPDTEVTTALVVA
ERIRAAVAGHPLETSRGPVTITVSIGVATTLPTSPDITATINSADAALYRAKAEGRNRAVSG
>Mature_381_residues
ALDIRTIFFMLGLATLAAAVSLFLAQRVRLAVHGIECWAWGMACAACSGFLLAQRGALSPLLTVVAANSVLLLGLSLFWL
GMRRFVGRSCSPSWIFAAPAIALPFLATRYLDDSDPAGRISFLSIIVALLLFLTASELLRHGERGRLGRRWVALLLILHG
LFFCVRAILSATGTPLSDPFGSGRLAMMTGLEGFLFILFFTLGLVIMTSETLQAELNRQATRDPLTDVYNRRAFHDLSAH
ELARAQRSGRALALLLIDLDHFKQLNDRHGHHVGDLALKHFVARVAPCLRHQDIFARYGGEEFVVLLPDTEVTTALVVAE
RIRAAVAGHPLETSRGPVTITVSIGVATTLPTSPDITATINSADAALYRAKAEGRNRAVSG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=217, Percent_Identity=35.9447004608295, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787262, Length=163, Percent_Identity=39.8773006134969, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87082007, Length=280, Percent_Identity=33.9285714285714, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787802, Length=213, Percent_Identity=32.3943661971831, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI145693134, Length=186, Percent_Identity=36.5591397849462, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI87081881, Length=175, Percent_Identity=38.8571428571429, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787816, Length=161, Percent_Identity=34.1614906832298, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=32.9268292682927, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787541, Length=179, Percent_Identity=32.9608938547486, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788381, Length=172, Percent_Identity=31.9767441860465, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI87081977, Length=160, Percent_Identity=30, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87081974, Length=113, Percent_Identity=31.858407079646, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788956, Length=164, Percent_Identity=32.3170731707317, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 41447; Mature: 41316

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDIRTIFFMLGLATLAAAVSLFLAQRVRLAVHGIECWAWGMACAACSGFLLAQRGALS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
PLLTVVAANSVLLLGLSLFWLGMRRFVGRSCSPSWIFAAPAIALPFLATRYLDDSDPAGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCH
ISFLSIIVALLLFLTASELLRHGERGRLGRRWVALLLILHGLFFCVRAILSATGTPLSDP
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
FGSGRLAMMTGLEGFLFILFFTLGLVIMTSETLQAELNRQATRDPLTDVYNRRAFHDLSA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHH
HELARAQRSGRALALLLIDLDHFKQLNDRHGHHVGDLALKHFVARVAPCLRHQDIFARYG
HHHHHHHHCCCEEEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GEEFVVLLPDTEVTTALVVAERIRAAVAGHPLETSRGPVTITVSIGVATTLPTSPDITAT
CCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECEEEECCCCCCCEEE
INSADAALYRAKAEGRNRAVSG
ECCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ALDIRTIFFMLGLATLAAAVSLFLAQRVRLAVHGIECWAWGMACAACSGFLLAQRGALS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
PLLTVVAANSVLLLGLSLFWLGMRRFVGRSCSPSWIFAAPAIALPFLATRYLDDSDPAGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCH
ISFLSIIVALLLFLTASELLRHGERGRLGRRWVALLLILHGLFFCVRAILSATGTPLSDP
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
FGSGRLAMMTGLEGFLFILFFTLGLVIMTSETLQAELNRQATRDPLTDVYNRRAFHDLSA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHH
HELARAQRSGRALALLLIDLDHFKQLNDRHGHHVGDLALKHFVARVAPCLRHQDIFARYG
HHHHHHHHCCCEEEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GEEFVVLLPDTEVTTALVVAERIRAAVAGHPLETSRGPVTITVSIGVATTLPTSPDITAT
CCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECEEEECCCCCCCEEE
INSADAALYRAKAEGRNRAVSG
ECCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA