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The map label for this gene is yeaP [H]

Identifier: 119898903

GI number: 119898903

Start: 2895707

End: 2896642

Strand: Reverse

Name: yeaP [H]

Synonym: azo2612

Alternate gene names: 119898903

Gene position: 2896642-2895707 (Counterclockwise)

Preceding gene: 119898904

Following gene: 119898902

Centisome position: 66.19

GC content: 69.55

Gene sequence:

>936_bases
ATGATCGACGTCGCATTCAACGACTTCCAGAGCGCGGCCCACGCGGTGCTCGCCTACCTGCACAAGCGCTTCGGCTTCGG
ATTGTGGATGGTGACCCGCACCGAAGGCGAGGACTGGATCGTGCTGCAGGCCGAGGACCACGGCTACGGCGTCAAGGCCG
GCTCGGTGTTCCGCTGGGCGGATTCCTTCTGCTCGGAGATGGTCAAGGGCAACGGCCCGCGCATCGCGCCGGATTGCGAC
GCGGTGGCGGTGTATGCGGCGGCACCGATCGGCCGCCAGGTGCCGATCAAGGCCTACATCGGCGTGCCGCTGACCAAGGC
GGACGGCAGCCTCTTCGGCACCCTGTGCGCGATCGACCCGGTCAGCCAGTCGCCGGAGATCGTGGCCGAGCAGCACCTCG
TGGAACTGCTCGCCCGCCTGCTCAGCACCATCCTGCACGCGGACCTGCTGCTGTCGCACGAGATCCGCCGCAACGAACGC
CTCGAACTGGAGGCCCAGACCGACCCGCTCACCGGCCTCTTCAACCGCCGCGCATGGGACCAGATGCTGCACCGGGAGGA
GGAGCGCTGCGTGCGCTTCGGCCATCCTGCCGCGGCGGTGGTCGTCGACCTCGCCGGCCTGCAACACGTTAACGACAGCG
AAGGCCACGCCGCCGGCGACGCCCTGATCGTCTGTGCCGCGCAGGCGCTGCGGGCCTCCGTGCGCGAGGTGGACATCCTT
GCCCGCCTCGGCGGCGACGAGTTCGGCGTGGTGGCGGTGGAGTGCAACGAAGCCGGCGGGCACGCGCTGGTGGCGCGGTT
GCGAGCCGCCTTTGCGGCCTATGGTGTGGATGCGTCGATCGGCATGGCAATGCGCGATCCCACCTGGGGCCTGGACGCGG
CCTGGCAGCGCGCCGACCGCCTGATGTACAGCGAAAAGCGCCGGCTGCGCGGTTAA

Upstream 100 bases:

>100_bases
GTGGATGGGCTGCACGACCGCTGGCAGGACAGCCCCGCGCTGCATCGGCACCTGTAGGCGCTGCGCCGGCGCTCGGCGGG
CCGGGGCAGGGGAGACACGC

Downstream 100 bases:

>100_bases
GGCGCGCAGCCGCAAGTCGCCTTCTCGGGTTCGTTTCGGTCTGTATCGAGATTGATTCTTATTTGTGCATGACGCTACAA
TCCGCGCCAGCCAGCCCGCC

Product: GAF/GGDEF-domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MIDVAFNDFQSAAHAVLAYLHKRFGFGLWMVTRTEGEDWIVLQAEDHGYGVKAGSVFRWADSFCSEMVKGNGPRIAPDCD
AVAVYAAAPIGRQVPIKAYIGVPLTKADGSLFGTLCAIDPVSQSPEIVAEQHLVELLARLLSTILHADLLLSHEIRRNER
LELEAQTDPLTGLFNRRAWDQMLHREEERCVRFGHPAAAVVVDLAGLQHVNDSEGHAAGDALIVCAAQALRASVREVDIL
ARLGGDEFGVVAVECNEAGGHALVARLRAAFAAYGVDASIGMAMRDPTWGLDAAWQRADRLMYSEKRRLRG

Sequences:

>Translated_311_residues
MIDVAFNDFQSAAHAVLAYLHKRFGFGLWMVTRTEGEDWIVLQAEDHGYGVKAGSVFRWADSFCSEMVKGNGPRIAPDCD
AVAVYAAAPIGRQVPIKAYIGVPLTKADGSLFGTLCAIDPVSQSPEIVAEQHLVELLARLLSTILHADLLLSHEIRRNER
LELEAQTDPLTGLFNRRAWDQMLHREEERCVRFGHPAAAVVVDLAGLQHVNDSEGHAAGDALIVCAAQALRASVREVDIL
ARLGGDEFGVVAVECNEAGGHALVARLRAAFAAYGVDASIGMAMRDPTWGLDAAWQRADRLMYSEKRRLRG
>Mature_311_residues
MIDVAFNDFQSAAHAVLAYLHKRFGFGLWMVTRTEGEDWIVLQAEDHGYGVKAGSVFRWADSFCSEMVKGNGPRIAPDCD
AVAVYAAAPIGRQVPIKAYIGVPLTKADGSLFGTLCAIDPVSQSPEIVAEQHLVELLARLLSTILHADLLLSHEIRRNER
LELEAQTDPLTGLFNRRAWDQMLHREEERCVRFGHPAAAVVVDLAGLQHVNDSEGHAAGDALIVCAAQALRASVREVDIL
ARLGGDEFGVVAVECNEAGGHALVARLRAAFAAYGVDASIGMAMRDPTWGLDAAWQRADRLMYSEKRRLRG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger (Probable). Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: COG2203

COG function: function code T; FOG: GAF domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI87081977, Length=227, Percent_Identity=30.8370044052863, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1786584, Length=94, Percent_Identity=36.1702127659575, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693134, Length=158, Percent_Identity=33.5443037974684, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI87081974, Length=172, Percent_Identity=29.0697674418605, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788381, Length=176, Percent_Identity=30.1136363636364, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=25.2873563218391, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=30.9941520467836, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787262, Length=104, Percent_Identity=33.6538461538462, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87082007, Length=159, Percent_Identity=30.188679245283, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003018 [H]

Pfam domain/function: PF01590 GAF; PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 33923; Mature: 33923

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVAFNDFQSAAHAVLAYLHKRFGFGLWMVTRTEGEDWIVLQAEDHGYGVKAGSVFRWA
CCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHH
DSFCSEMVKGNGPRIAPDCDAVAVYAAAPIGRQVPIKAYIGVPLTKADGSLFGTLCAIDP
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCEEEECCCCCEEHEEECCC
VSQSPEIVAEQHLVELLARLLSTILHADLLLSHEIRRNERLELEAQTDPLTGLFNRRAWD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
QMLHREEERCVRFGHPAAAVVVDLAGLQHVNDSEGHAAGDALIVCAAQALRASVREVDIL
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ARLGGDEFGVVAVECNEAGGHALVARLRAAFAAYGVDASIGMAMRDPTWGLDAAWQRADR
HHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHH
LMYSEKRRLRG
HHHHHHHHCCC
>Mature Secondary Structure
MIDVAFNDFQSAAHAVLAYLHKRFGFGLWMVTRTEGEDWIVLQAEDHGYGVKAGSVFRWA
CCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHH
DSFCSEMVKGNGPRIAPDCDAVAVYAAAPIGRQVPIKAYIGVPLTKADGSLFGTLCAIDP
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCEEEECCCCCEEHEEECCC
VSQSPEIVAEQHLVELLARLLSTILHADLLLSHEIRRNERLELEAQTDPLTGLFNRRAWD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
QMLHREEERCVRFGHPAAAVVVDLAGLQHVNDSEGHAAGDALIVCAAQALRASVREVDIL
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ARLGGDEFGVVAVECNEAGGHALVARLRAAFAAYGVDASIGMAMRDPTWGLDAAWQRADR
HHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHH
LMYSEKRRLRG
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]