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The map label for this gene is cca2 [H]

Identifier: 119898840

GI number: 119898840

Start: 2818660

End: 2819562

Strand: Reverse

Name: cca2 [H]

Synonym: azo2549

Alternate gene names: 119898840

Gene position: 2819562-2818660 (Counterclockwise)

Preceding gene: 119898852

Following gene: 119898839

Centisome position: 64.43

GC content: 72.54

Gene sequence:

>903_bases
ATGAGCACCGATCCTTCCCGCGGCCTGTTGTCGGCCACCCTGGGCGCCGTGCTGGCCAACCCCGCACTCAATGTGCTGCG
CATCGCCGCGATGGCCGCCGGGCCGGATGGCGAGGTCGACCCCGAGACCGTGGAGATGATGCGGGCCCAGGTGGCGGCCG
GCGCGCTTGCCACCCTGGCCCCGGCCGCCGCGTGGCCCGAACTGGAGCGCGGCCTGCTCGCGGCGGCGCCCTCGCGCATG
CTGCGCGCGCTGCGAGACTGCGGCGCGCTGGCGGTGCTGCTGCCCGAAGTCGATGCCCTGTTTGGCGTGCCGCAAAGCGC
GGACGACCCGGCCGAGGTCGACATCGGCGACCACATCCTGCGCGTCGTCGACGAGGCCGCGCGCTGCAACGCGCCGTTGG
CGGTGCGCTTCGCGGCGCTGGTGTTCAACGTCGGCAAGGCAGATTCGCCGCGCGAGCACCTGCCCGCGCACTACAAGCAC
ATCGAGCGCGGCTGCCCGCGCATCGAAGCGATCTGCGCCCGCTTCGGCGTTGCGGCGGAATTTCTCGACCTTGCGCTGCT
GGCGATCGCCGAATGCGAGCGCGTGCATCGCGCGGCCGAAATGCGCGCCGGCTCGATCGCCGCGATGCTGGAGCGGGTGG
ATGCCTTTGACCGCCCGGCGCGCTTCGAACAACTGCTCACCTTGTGTACCTGCGACTTCCGCGCCTTCCCCGGCCGCGCG
TCGCTGGTGTATCCGAAAGCGCCGATGCTGCGCGTGGCGCTGCGCGCCTGCCTGGCGGTGGATGAAGGCGAGCTGGCCGA
CGAACACGAGGACGAGGCTGAGTTCGCCGCCGCGCTGCTCGAAGCCCGCGCACTGGCGGTGGCGGCGGCGCTGCGGTCCG
AGCGCTGGGCCGATGCGGCCTGA

Upstream 100 bases:

>100_bases
CGGCGCCGCGGGGGCGACAATCCGCACTAACCGCCGCCGCGGGGCTTGCTGCGGCGCAATCACGCGCTACCATCATGGTT
TTTCGCCCCCCGAGTTCGCG

Downstream 100 bases:

>100_bases
GCGGCGCGCCAGCCAGACGCACGCCGCTAGTAAGATCACGTTTTCCAGAACCACGAGAACCACGGAGCTGCCATGAACCC
TTTCCAGTCCGGCATCCTTG

Product: tRNA nucleotidyltransferase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSTDPSRGLLSATLGAVLANPALNVLRIAAMAAGPDGEVDPETVEMMRAQVAAGALATLAPAAAWPELERGLLAAAPSRM
LRALRDCGALAVLLPEVDALFGVPQSADDPAEVDIGDHILRVVDEAARCNAPLAVRFAALVFNVGKADSPREHLPAHYKH
IERGCPRIEAICARFGVAAEFLDLALLAIAECERVHRAAEMRAGSIAAMLERVDAFDRPARFEQLLTLCTCDFRAFPGRA
SLVYPKAPMLRVALRACLAVDEGELADEHEDEAEFAAALLEARALAVAAALRSERWADAA

Sequences:

>Translated_300_residues
MSTDPSRGLLSATLGAVLANPALNVLRIAAMAAGPDGEVDPETVEMMRAQVAAGALATLAPAAAWPELERGLLAAAPSRM
LRALRDCGALAVLLPEVDALFGVPQSADDPAEVDIGDHILRVVDEAARCNAPLAVRFAALVFNVGKADSPREHLPAHYKH
IERGCPRIEAICARFGVAAEFLDLALLAIAECERVHRAAEMRAGSIAAMLERVDAFDRPARFEQLLTLCTCDFRAFPGRA
SLVYPKAPMLRVALRACLAVDEGELADEHEDEAEFAAALLEARALAVAAALRSERWADAA
>Mature_299_residues
STDPSRGLLSATLGAVLANPALNVLRIAAMAAGPDGEVDPETVEMMRAQVAAGALATLAPAAAWPELERGLLAAAPSRML
RALRDCGALAVLLPEVDALFGVPQSADDPAEVDIGDHILRVVDEAARCNAPLAVRFAALVFNVGKADSPREHLPAHYKHI
ERGCPRIEAICARFGVAAEFLDLALLAIAECERVHRAAEMRAGSIAAMLERVDAFDRPARFEQLLTLCTCDFRAFPGRAS
LVYPKAPMLRVALRACLAVDEGELADEHEDEAEFAAALLEARALAVAAALRSERWADAA

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=267, Percent_Identity=34.8314606741573, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 31858; Mature: 31727

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDPSRGLLSATLGAVLANPALNVLRIAAMAAGPDGEVDPETVEMMRAQVAAGALATLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
PAAAWPELERGLLAAAPSRMLRALRDCGALAVLLPEVDALFGVPQSADDPAEVDIGDHIL
HHHCCHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
RVVDEAARCNAPLAVRFAALVFNVGKADSPREHLPAHYKHIERGCPRIEAICARFGVAAE
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHCCHHHHHHHHHHHHHHH
FLDLALLAIAECERVHRAAEMRAGSIAAMLERVDAFDRPARFEQLLTLCTCDFRAFPGRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
SLVYPKAPMLRVALRACLAVDEGELADEHEDEAEFAAALLEARALAVAAALRSERWADAA
CEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STDPSRGLLSATLGAVLANPALNVLRIAAMAAGPDGEVDPETVEMMRAQVAAGALATLA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
PAAAWPELERGLLAAAPSRMLRALRDCGALAVLLPEVDALFGVPQSADDPAEVDIGDHIL
HHHCCHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
RVVDEAARCNAPLAVRFAALVFNVGKADSPREHLPAHYKHIERGCPRIEAICARFGVAAE
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHCCHHHHHHHHHHHHHHH
FLDLALLAIAECERVHRAAEMRAGSIAAMLERVDAFDRPARFEQLLTLCTCDFRAFPGRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCC
SLVYPKAPMLRVALRACLAVDEGELADEHEDEAEFAAALLEARALAVAAALRSERWADAA
CEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA