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The map label for this gene is nac [H]

Identifier: 119898837

GI number: 119898837

Start: 2814613

End: 2815779

Strand: Direct

Name: nac [H]

Synonym: azo2546

Alternate gene names: 119898837

Gene position: 2814613-2815779 (Clockwise)

Preceding gene: 119898824

Following gene: 119898838

Centisome position: 64.32

GC content: 70.44

Gene sequence:

>1167_bases
ATGGGGATGGAATACGTTGTAGTCGTTATCGAAGGCCGCGTGCGATCGGTCGGCGCGGCGGGGCCCTATGCTGCCGGAAA
AGCGGCGCCGGATGCGAGCCGGCGCACGCGGGCCGCCGCCGACGCCGCGCCGCGGATGACAACGGCACGCGCGAGGGAAT
CCGTCCCCATCATGGGGCAGGCCCCCGCTGCCGACCGCCTGCCGGCGCGATATCAGCTATGCCGCTTTGCGATAGCTGAC
CCCCTGACGGCTGCGCGCTACACTGCCCGCATGGACCTGAAGCAACTCGAATACTTCGTGCGCGTGGCCGAACTCGGCAG
CTTCACGCGTGCCTCGATCGCGCTCGACATCGCCCAGCCGGCGCTGTCGCGCCAGGTGCGGCTGCTGGAAGTGGAGCTGC
ACCAGAACCTGCTGGTGCGCAACGGCCGCGGCGTCACGCTCACCGAGGCGGGCAAGGTGCTGCTGGAGCACAGCCGCGGC
GTGCTGCACCAGATCGAGCGCATCCGTGAGGAACTGGAGCGGGTGCGCGGCACGCCGTCGGGCCGGGTCGCGGTGGGCTT
GCCGCCCAGCCTGTCGCGCGTGCTGACGGTGCCGGTGACGCGCGAATTCCGCGCGCGCATGCCCGGCGCCACGCTGTCGA
TCACCGAAGGGCTGTCGGTGACCATGCGCGAGGCGCTGATGAACGGCCGGCTCGACATCGCCCTGCTCTACAACGCCGTG
CCCTCCACCGACATCGACCTGTTCCCGCTGCTGGACGAACCGCTGTTCCTCGTCCAGCGCGCCGATCACGGCAGCACCGC
GCCCATCCCGCTGCGCGAACTCGCGGACCATCCGCTGGTGATCCCGAGCCGCCCCAATGCGCTGCGCATGCTGGTGGAAA
CCGAACTCGCCAACCTCGGCTGCCGGCCCCGGATCGCGATCGAGATCGACGGCGTGGCGCCGATCCTCGGCCTGGTGGCC
GATGGCCTGGGCAGCGCGGTGCTGTCGATGAACGCGGTCGCCACCTCGGCCAAGGCGGCCGAATTCGTCGTGCGTCCCTT
CATCGAGCCCGGGCTGCAGTCCAGGCTGTCGATGGCGGTCAGCTCGCGCCGGCCGACCACCGGCACCCAGCAGGCCATGC
TCGAACTGCTGCGCGAAATCGCCCCCCGCCTGATCACGCCGGCATGA

Upstream 100 bases:

>100_bases
TTCGGTGATCGCCGGCAGCGCCGGCAGGTAGAGGTCGGTGGTCAGCGGCTGGATGCCCAGCAGCAGCGACAGCAGCGCGA
CGACGAGAGCGGGATTCATG

Downstream 100 bases:

>100_bases
TCGCCGCCATGCGGGCGGAATGCCGCAGATGTCACGGCGGCCCTCAAGAAATCGCAAAACCATTCCGCTAAAGCAGCCGT
CGCCCCTGTCGGGGCCGCGA

Product: putative nitrogen assimilation regulatory protein

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MGMEYVVVVIEGRVRSVGAAGPYAAGKAAPDASRRTRAAADAAPRMTTARARESVPIMGQAPAADRLPARYQLCRFAIAD
PLTAARYTARMDLKQLEYFVRVAELGSFTRASIALDIAQPALSRQVRLLEVELHQNLLVRNGRGVTLTEAGKVLLEHSRG
VLHQIERIREELERVRGTPSGRVAVGLPPSLSRVLTVPVTREFRARMPGATLSITEGLSVTMREALMNGRLDIALLYNAV
PSTDIDLFPLLDEPLFLVQRADHGSTAPIPLRELADHPLVIPSRPNALRMLVETELANLGCRPRIAIEIDGVAPILGLVA
DGLGSAVLSMNAVATSAKAAEFVVRPFIEPGLQSRLSMAVSSRRPTTGTQQAMLELLREIAPRLITPA

Sequences:

>Translated_388_residues
MGMEYVVVVIEGRVRSVGAAGPYAAGKAAPDASRRTRAAADAAPRMTTARARESVPIMGQAPAADRLPARYQLCRFAIAD
PLTAARYTARMDLKQLEYFVRVAELGSFTRASIALDIAQPALSRQVRLLEVELHQNLLVRNGRGVTLTEAGKVLLEHSRG
VLHQIERIREELERVRGTPSGRVAVGLPPSLSRVLTVPVTREFRARMPGATLSITEGLSVTMREALMNGRLDIALLYNAV
PSTDIDLFPLLDEPLFLVQRADHGSTAPIPLRELADHPLVIPSRPNALRMLVETELANLGCRPRIAIEIDGVAPILGLVA
DGLGSAVLSMNAVATSAKAAEFVVRPFIEPGLQSRLSMAVSSRRPTTGTQQAMLELLREIAPRLITPA
>Mature_387_residues
GMEYVVVVIEGRVRSVGAAGPYAAGKAAPDASRRTRAAADAAPRMTTARARESVPIMGQAPAADRLPARYQLCRFAIADP
LTAARYTARMDLKQLEYFVRVAELGSFTRASIALDIAQPALSRQVRLLEVELHQNLLVRNGRGVTLTEAGKVLLEHSRGV
LHQIERIREELERVRGTPSGRVAVGLPPSLSRVLTVPVTREFRARMPGATLSITEGLSVTMREALMNGRLDIALLYNAVP
STDIDLFPLLDEPLFLVQRADHGSTAPIPLRELADHPLVIPSRPNALRMLVETELANLGCRPRIAIEIDGVAPILGLVAD
GLGSAVLSMNAVATSAKAAEFVVRPFIEPGLQSRLSMAVSSRRPTTGTQQAMLELLREIAPRLITPA

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=239, Percent_Identity=35.5648535564854, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI157672245, Length=228, Percent_Identity=31.140350877193, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI145693105, Length=241, Percent_Identity=28.2157676348548, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787601, Length=233, Percent_Identity=26.6094420600858, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788887, Length=266, Percent_Identity=31.5789473684211, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790399, Length=262, Percent_Identity=28.6259541984733, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787879, Length=243, Percent_Identity=31.6872427983539, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI2367136, Length=244, Percent_Identity=28.2786885245902, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788748, Length=289, Percent_Identity=26.643598615917, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787806, Length=239, Percent_Identity=28.0334728033473, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789639, Length=126, Percent_Identity=30.952380952381, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789506, Length=230, Percent_Identity=25.6521739130435, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787530, Length=307, Percent_Identity=25.4071661237785, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87082132, Length=306, Percent_Identity=25.4901960784314, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788481, Length=252, Percent_Identity=25.3968253968254, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786401, Length=199, Percent_Identity=28.1407035175879, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790208, Length=145, Percent_Identity=31.7241379310345, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787589, Length=266, Percent_Identity=28.1954887218045, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790262, Length=286, Percent_Identity=25.8741258741259, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081904, Length=226, Percent_Identity=27.8761061946903, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI145693193, Length=126, Percent_Identity=30.1587301587302, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 41936; Mature: 41805

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMEYVVVVIEGRVRSVGAAGPYAAGKAAPDASRRTRAAADAAPRMTTARARESVPIMGQ
CCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCC
APAADRLPARYQLCRFAIADPLTAARYTARMDLKQLEYFVRVAELGSFTRASIALDIAQP
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEHHHH
ALSRQVRLLEVELHQNLLVRNGRGVTLTEAGKVLLEHSRGVLHQIERIREELERVRGTPS
HHHHCEEEEEEEHHHHHHEECCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
GRVAVGLPPSLSRVLTVPVTREFRARMPGATLSITEGLSVTMREALMNGRLDIALLYNAV
CEEEEECCCCCCCEEECCCCHHHHHHCCCCEEEECCCCHHHHHHHHHCCCEEEEEEECCC
PSTDIDLFPLLDEPLFLVQRADHGSTAPIPLRELADHPLVIPSRPNALRMLVETELANLG
CCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHCCCCEEECCCCHHHHHHHHHHHHHCC
CRPRIAIEIDGVAPILGLVADGLGSAVLSMNAVATSAKAAEFVVRPFIEPGLQSRLSMAV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
SSRRPTTGTQQAMLELLREIAPRLITPA
HCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GMEYVVVVIEGRVRSVGAAGPYAAGKAAPDASRRTRAAADAAPRMTTARARESVPIMGQ
CCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCCCCC
APAADRLPARYQLCRFAIADPLTAARYTARMDLKQLEYFVRVAELGSFTRASIALDIAQP
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEEEHHHH
ALSRQVRLLEVELHQNLLVRNGRGVTLTEAGKVLLEHSRGVLHQIERIREELERVRGTPS
HHHHCEEEEEEEHHHHHHEECCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
GRVAVGLPPSLSRVLTVPVTREFRARMPGATLSITEGLSVTMREALMNGRLDIALLYNAV
CEEEEECCCCCCCEEECCCCHHHHHHCCCCEEEECCCCHHHHHHHHHCCCEEEEEEECCC
PSTDIDLFPLLDEPLFLVQRADHGSTAPIPLRELADHPLVIPSRPNALRMLVETELANLG
CCCCCEEEECCCCCEEEEEECCCCCCCCCCHHHHCCCCEEECCCCHHHHHHHHHHHHHCC
CRPRIAIEIDGVAPILGLVADGLGSAVLSMNAVATSAKAAEFVVRPFIEPGLQSRLSMAV
CCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
SSRRPTTGTQQAMLELLREIAPRLITPA
HCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8458853 [H]