Click here to switch to the map view.

The map label for this gene is bcr1 [H]

Identifier: 119898836

GI number: 119898836

Start: 2813370

End: 2814611

Strand: Reverse

Name: bcr1 [H]

Synonym: azo2545

Alternate gene names: 119898836

Gene position: 2814611-2813370 (Counterclockwise)

Preceding gene: 119898839

Following gene: 119898835

Centisome position: 64.32

GC content: 72.87

Gene sequence:

>1242_bases
ATGAATCCCGCTCTCGTCGTCGCGCTGCTGTCGCTGCTGCTGGGCATCCAGCCGCTGACCACCGACCTCTACCTGCCGGC
GCTGCCGGCGATCACCGAAGCCTTCGGCGCGCCGCTGCAGCAGGCGCAGTTCACGCTGGCCGCGCTGTTGCTGGCGTTCG
GCGCCTCGCAGCTGGTGTGGGGGCCGCTGTCCGACCGCTACGGCCGCCGTCCGGTGCTGCTGGCCGGGCTCGTCGCCTAT
ACCGTGGCCGCGGTGGGCAGCAGCGCCGCCGGCTCGATGGAGGCGCTGATCGCGTGGCGGGTGGTGCAGGGCGCGGCGAT
GGGGGCGGCGGTGATGTGCGCGCGCGCCATGGTGCGCGACCTCTATTCACCGGAGCTGGGGCCGCGGGTGATGAGCAAGG
GGCAGACCGGGCTGGGCGTGCTCGCCTGCCTCAGCGCCCCGGTCGGCGGCATCATTGCCGAATTCCTCGGCTGGCGCGCC
ACGCTGCTGTCGCTGGCGCTGGTGGGCGCGGGGACGCTGGCGCTGGTCGCCACGCGCTTCGTCGAACCCTTGCCGGCGCG
TAACCCGCAGGCGCTGCATCCGCGCACGTTGCTGCGCACCTGGGGCGGCATCCTCGCCCATCCGGCGTTCCGGGCGTTCG
GGCTGCTGTCGGCGTTTTCGTACGCGGGCCTGTTCACCTTCCTGGCCTCGTCGTCCTTCGTGTTCATCAAGGTGCTGGGG
CTCAGCCGCATCGGCTACGGTGCGGTGATGCTCACGACCTCGATCGCCTACATCAGCGGCACTTTCCTGTGCCGCCGCCT
GCTCGGCCGTCTGGGGCTGCGCCGCACGGTGGCGGTGGGCGGCTTGCTGACGCTGAGCGGCGGGGGCCTGGTCGCCGGGC
TGGCGCTGCTCGGGCTGCGCGAGGTGTGGAGCATCCTGCTGCCCTTCCTGATCTACATGCTGGGTCACGGCATCCACCAA
CCCTGCGGGCAGAGCGGCGCGGTGGCGCCGTTTCCGCGCGCAGCCGGGGCGGCCTCCGCGCTGAGCGGTTTCCTGATGAT
GGTGACCACCTTTGCGATGGGGCTGTGGCTGGGCAGCCACATGGGCGAGGGGGTGTGGCCGCTGACCCACGGGCTGGGCT
TCTGGAGCGTCGCGATCGCGGCGGTGGCGTGGGTGCTGGTGCCGCGCGCGATCGACGCGGGCGCCGCGGCTACCGCGCGC
GAAACCGCGGCGGACGGGGGCACGGCGGACACGCCGCGCTAG

Upstream 100 bases:

>100_bases
GCTCGCATCCGGCGCCGCTTTTCCGGCAGCATAGGGCCCCGCCGCGCCGACCGATCGCACGCGGCCTTCGATAACGACTA
CAACGTATTCCATCCCCATC

Downstream 100 bases:

>100_bases
GCCGCTCTGTCTTTCGGACTACGTATCGGTGAATAGCTGGGGTCCGGCTATCGCAAACCGAGATAGCTGCTAGAGCGTTT
GCCTTCTATTGTGGGGAGGG

Product: putative bicyclomycin resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MNPALVVALLSLLLGIQPLTTDLYLPALPAITEAFGAPLQQAQFTLAALLLAFGASQLVWGPLSDRYGRRPVLLAGLVAY
TVAAVGSSAAGSMEALIAWRVVQGAAMGAAVMCARAMVRDLYSPELGPRVMSKGQTGLGVLACLSAPVGGIIAEFLGWRA
TLLSLALVGAGTLALVATRFVEPLPARNPQALHPRTLLRTWGGILAHPAFRAFGLLSAFSYAGLFTFLASSSFVFIKVLG
LSRIGYGAVMLTTSIAYISGTFLCRRLLGRLGLRRTVAVGGLLTLSGGGLVAGLALLGLREVWSILLPFLIYMLGHGIHQ
PCGQSGAVAPFPRAAGAASALSGFLMMVTTFAMGLWLGSHMGEGVWPLTHGLGFWSVAIAAVAWVLVPRAIDAGAAATAR
ETAADGGTADTPR

Sequences:

>Translated_413_residues
MNPALVVALLSLLLGIQPLTTDLYLPALPAITEAFGAPLQQAQFTLAALLLAFGASQLVWGPLSDRYGRRPVLLAGLVAY
TVAAVGSSAAGSMEALIAWRVVQGAAMGAAVMCARAMVRDLYSPELGPRVMSKGQTGLGVLACLSAPVGGIIAEFLGWRA
TLLSLALVGAGTLALVATRFVEPLPARNPQALHPRTLLRTWGGILAHPAFRAFGLLSAFSYAGLFTFLASSSFVFIKVLG
LSRIGYGAVMLTTSIAYISGTFLCRRLLGRLGLRRTVAVGGLLTLSGGGLVAGLALLGLREVWSILLPFLIYMLGHGIHQ
PCGQSGAVAPFPRAAGAASALSGFLMMVTTFAMGLWLGSHMGEGVWPLTHGLGFWSVAIAAVAWVLVPRAIDAGAAATAR
ETAADGGTADTPR
>Mature_413_residues
MNPALVVALLSLLLGIQPLTTDLYLPALPAITEAFGAPLQQAQFTLAALLLAFGASQLVWGPLSDRYGRRPVLLAGLVAY
TVAAVGSSAAGSMEALIAWRVVQGAAMGAAVMCARAMVRDLYSPELGPRVMSKGQTGLGVLACLSAPVGGIIAEFLGWRA
TLLSLALVGAGTLALVATRFVEPLPARNPQALHPRTLLRTWGGILAHPAFRAFGLLSAFSYAGLFTFLASSSFVFIKVLG
LSRIGYGAVMLTTSIAYISGTFLCRRLLGRLGLRRTVAVGGLLTLSGGGLVAGLALLGLREVWSILLPFLIYMLGHGIHQ
PCGQSGAVAPFPRAAGAASALSGFLMMVTTFAMGLWLGSHMGEGVWPLTHGLGFWSVAIAAVAWVLVPRAIDAGAAATAR
ETAADGGTADTPR

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=386, Percent_Identity=30.0518134715026, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87082312, Length=333, Percent_Identity=30.03003003003, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI48994889, Length=338, Percent_Identity=28.4023668639053, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790146, Length=365, Percent_Identity=24.1095890410959, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787065, Length=262, Percent_Identity=25.5725190839695, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319480, Length=311, Percent_Identity=23.1511254019293, Blast_Score=66, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6324264, Length=175, Percent_Identity=29.7142857142857, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42687; Mature: 42687

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPALVVALLSLLLGIQPLTTDLYLPALPAITEAFGAPLQQAQFTLAALLLAFGASQLVW
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHEE
GPLSDRYGRRPVLLAGLVAYTVAAVGSSAAGSMEALIAWRVVQGAAMGAAVMCARAMVRD
CCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYSPELGPRVMSKGQTGLGVLACLSAPVGGIIAEFLGWRATLLSLALVGAGTLALVATRF
HHCCCCCHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VEPLPARNPQALHPRTLLRTWGGILAHPAFRAFGLLSAFSYAGLFTFLASSSFVFIKVLG
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHH
LSRIGYGAVMLTTSIAYISGTFLCRRLLGRLGLRRTVAVGGLLTLSGGGLVAGLALLGLR
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEEECCCHHHHHHHHHHHH
EVWSILLPFLIYMLGHGIHQPCGQSGAVAPFPRAAGAASALSGFLMMVTTFAMGLWLGSH
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MGEGVWPLTHGLGFWSVAIAAVAWVLVPRAIDAGAAATARETAADGGTADTPR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNPALVVALLSLLLGIQPLTTDLYLPALPAITEAFGAPLQQAQFTLAALLLAFGASQLVW
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHEE
GPLSDRYGRRPVLLAGLVAYTVAAVGSSAAGSMEALIAWRVVQGAAMGAAVMCARAMVRD
CCCHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYSPELGPRVMSKGQTGLGVLACLSAPVGGIIAEFLGWRATLLSLALVGAGTLALVATRF
HHCCCCCHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VEPLPARNPQALHPRTLLRTWGGILAHPAFRAFGLLSAFSYAGLFTFLASSSFVFIKVLG
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHH
LSRIGYGAVMLTTSIAYISGTFLCRRLLGRLGLRRTVAVGGLLTLSGGGLVAGLALLGLR
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEEECCCHHHHHHHHHHHH
EVWSILLPFLIYMLGHGIHQPCGQSGAVAPFPRAAGAASALSGFLMMVTTFAMGLWLGSH
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
MGEGVWPLTHGLGFWSVAIAAVAWVLVPRAIDAGAAATARETAADGGTADTPR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]