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The map label for this gene is camA [H]

Identifier: 119898820

GI number: 119898820

Start: 2796181

End: 2797398

Strand: Direct

Name: camA [H]

Synonym: azo2529

Alternate gene names: 119898820

Gene position: 2796181-2797398 (Clockwise)

Preceding gene: 119898819

Following gene: 119898821

Centisome position: 63.9

GC content: 71.26

Gene sequence:

>1218_bases
GTGGTCATCGTCGGCGCCGGCCAGTCCGGGCTGCAGGTGGCCGAATCGCTGCGCAGCGAGGGCTATGCCAGCGTGATCGT
CATGATCGGCGACGAGCCCTGCCCGCCCTACCATCGCCCGCCGCTGTCCAAGGCCTATCTCGCCGGTGAGGCGAGCGAGG
CGCAGCTCACCATCCGCGCGCCCGAGGCGCTGGCGAAGAAGCAGATCGAGTTCGTCGCCGGCGTCGGCGTGGAGTCCATC
GACCGCGCCAACACCGTGCTCACGCTGACCGACGGCCGCAAGCTCGCCTACGCCGCGCTCGCGCTCGCCACCGGCTCGCG
CGCGCGGCCGCTGCCGGTGCCCGGCGCCGACCTCGCCAACGTGTTCGCGCTGCGCACGCTGGAGGACACCCGCCGCATCG
CCGCCGCGCTGGCCGCGGCACAGCGCGTGGTGGTGATCGGCGGCGGCTTCATCGGCCTGGAATTCGCCGCGGTCGCCGCC
AAGCAGGGCAAACAGGTCACCGTGCTGGAAGCCGCCGGGCGGTTGATGGCGCGGGTGGTGGCACCGCCGGTGTCGGATTT
CTACGCCGCGCTGCACCGCGGCCACGGCGTCGCCATCGAACTCGGTGCCGCGGTGTCGGCGCTGCGCGGCAAGGCCGGCC
CGGCGCAGGACGCGGTCGCCGCCGTATGCACCGCCGACGGCCGCGAATTCCCCGCCGACCTGGTGATCGTCGGCATCGGC
ATCCTGCCCAACAGCGAACTCGCGGAAGCTGCCGGGCTCGCCTGCGAGCGTGGCATCGTGGTCGATGGCTGCTCGCGCAC
CGCCGACCCCCGCATCGTCGCCAGCGGCGACTGCACCGCGCGGCGGCTGGAAGACGGCAGCCTGCTGCGACTGGAATCTG
TGCAGAACGCGGTGGAACAGGGCAAATCAGCCGCCGCCGCGCTGCTCGGCAAGGAACGCCGCTTCGACGCCGCCCCCTGG
TTCTGGTCCGACCAGTACGACGTCAAGCTGCAGATGGTGGGCTTGTCCGCCGGCTACGACCAGGTCGTCAGCCGTGGCGA
GGTCGAAAGCCAGCGCTTCTCGGTGTTCTACTTCCGCACCGGCAAACTGGTGGCGATCGATTCGATCAACCAGCCACAGG
TGCACATGCTCGGCCGCAAACTGCTCGACAAGGGCAACAGCCTGACGGCGGCACAGGCGGCAGACCCGGCATTCGATCTC
GCGGGGGCTGCGGCCTGA

Upstream 100 bases:

>100_bases
GGAAGGCATCGTGCTGCGCCTGCCCGTCAACCAGAGCTGAGGCATGGACGCGCCGCTCATCGCCGGCCCCGAGGGCGCCG
GCCTGCCGGGCAACGGCCCG

Downstream 100 bases:

>100_bases
AGCAGGGAGCGGCCAGCCGCTCCCTGCTTCAGGATCGGGTTCCACGGTCGGTCCTCGGGTTGGTTCCCCTCCAGGCCGGA
GGCCGAAACGCGGCGGCGGA

Product: putidaredoxin reductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MVIVGAGQSGLQVAESLRSEGYASVIVMIGDEPCPPYHRPPLSKAYLAGEASEAQLTIRAPEALAKKQIEFVAGVGVESI
DRANTVLTLTDGRKLAYAALALATGSRARPLPVPGADLANVFALRTLEDTRRIAAALAAAQRVVVIGGGFIGLEFAAVAA
KQGKQVTVLEAAGRLMARVVAPPVSDFYAALHRGHGVAIELGAAVSALRGKAGPAQDAVAAVCTADGREFPADLVIVGIG
ILPNSELAEAAGLACERGIVVDGCSRTADPRIVASGDCTARRLEDGSLLRLESVQNAVEQGKSAAAALLGKERRFDAAPW
FWSDQYDVKLQMVGLSAGYDQVVSRGEVESQRFSVFYFRTGKLVAIDSINQPQVHMLGRKLLDKGNSLTAAQAADPAFDL
AGAAA

Sequences:

>Translated_405_residues
MVIVGAGQSGLQVAESLRSEGYASVIVMIGDEPCPPYHRPPLSKAYLAGEASEAQLTIRAPEALAKKQIEFVAGVGVESI
DRANTVLTLTDGRKLAYAALALATGSRARPLPVPGADLANVFALRTLEDTRRIAAALAAAQRVVVIGGGFIGLEFAAVAA
KQGKQVTVLEAAGRLMARVVAPPVSDFYAALHRGHGVAIELGAAVSALRGKAGPAQDAVAAVCTADGREFPADLVIVGIG
ILPNSELAEAAGLACERGIVVDGCSRTADPRIVASGDCTARRLEDGSLLRLESVQNAVEQGKSAAAALLGKERRFDAAPW
FWSDQYDVKLQMVGLSAGYDQVVSRGEVESQRFSVFYFRTGKLVAIDSINQPQVHMLGRKLLDKGNSLTAAQAADPAFDL
AGAAA
>Mature_405_residues
MVIVGAGQSGLQVAESLRSEGYASVIVMIGDEPCPPYHRPPLSKAYLAGEASEAQLTIRAPEALAKKQIEFVAGVGVESI
DRANTVLTLTDGRKLAYAALALATGSRARPLPVPGADLANVFALRTLEDTRRIAAALAAAQRVVVIGGGFIGLEFAAVAA
KQGKQVTVLEAAGRLMARVVAPPVSDFYAALHRGHGVAIELGAAVSALRGKAGPAQDAVAAVCTADGREFPADLVIVGIG
ILPNSELAEAAGLACERGIVVDGCSRTADPRIVASGDCTARRLEDGSLLRLESVQNAVEQGKSAAAALLGKERRFDAAPW
FWSDQYDVKLQMVGLSAGYDQVVSRGEVESQRFSVFYFRTGKLVAIDSINQPQVHMLGRKLLDKGNSLTAAQAADPAFDL
AGAAA

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI65787454, Length=374, Percent_Identity=30.7486631016043, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI226437568, Length=374, Percent_Identity=30.7486631016043, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI21389617, Length=374, Percent_Identity=30.7486631016043, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI4757732, Length=355, Percent_Identity=23.6619718309859, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI22202629, Length=355, Percent_Identity=23.6619718309859, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1788892, Length=395, Percent_Identity=36.2025316455696, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1789065, Length=279, Percent_Identity=28.673835125448, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789765, Length=346, Percent_Identity=29.1907514450867, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=379, Percent_Identity=28.7598944591029, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI32564386, Length=345, Percent_Identity=27.2463768115942, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24639257, Length=402, Percent_Identity=26.3681592039801, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI281359715, Length=402, Percent_Identity=26.3681592039801, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI281359713, Length=402, Percent_Identity=26.3681592039801, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24639250, Length=402, Percent_Identity=26.3681592039801, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI18543267, Length=402, Percent_Identity=26.3681592039801, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24639252, Length=402, Percent_Identity=26.3681592039801, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24585130, Length=405, Percent_Identity=24.4444444444444, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24581020, Length=361, Percent_Identity=25.207756232687, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI28573993, Length=362, Percent_Identity=25.414364640884, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 42138; Mature: 42138

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIVGAGQSGLQVAESLRSEGYASVIVMIGDEPCPPYHRPPLSKAYLAGEASEAQLTIRA
CEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHCCCCCCCCEEEEEC
PEALAKKQIEFVAGVGVESIDRANTVLTLTDGRKLAYAALALATGSRARPLPVPGADLAN
HHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCCEEEHHHHHHHCCCCCCCCCCCCCHHHH
VFALRTLEDTRRIAAALAAAQRVVVIGGGFIGLEFAAVAAKQGKQVTVLEAAGRLMARVV
HHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHH
APPVSDFYAALHRGHGVAIELGAAVSALRGKAGPAQDAVAAVCTADGREFPADLVIVGIG
CCCHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCHHHHEEEEECCCCCCCCEEEEEEEE
ILPNSELAEAAGLACERGIVVDGCSRTADPRIVASGDCTARRLEDGSLLRLESVQNAVEQ
CCCCCHHHHHHCCHHCCCEEEECCCCCCCCCEEECCCCCCEECCCCCEEEHHHHHHHHHH
GKSAAAALLGKERRFDAAPWFWSDQYDVKLQMVGLSAGYDQVVSRGEVESQRFSVFYFRT
CHHHHHHHHCCCCCCCCCCCEECCCCEEEEEEEEECCCHHHHHCCCCCCCCEEEEEEEEC
GKLVAIDSINQPQVHMLGRKLLDKGNSLTAAQAADPAFDLAGAAA
CCEEEEECCCCCHHHHHHHHHHCCCCCEEEHHCCCCCHHHCCCCC
>Mature Secondary Structure
MVIVGAGQSGLQVAESLRSEGYASVIVMIGDEPCPPYHRPPLSKAYLAGEASEAQLTIRA
CEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCHHHCCCCCCCCEEEEEC
PEALAKKQIEFVAGVGVESIDRANTVLTLTDGRKLAYAALALATGSRARPLPVPGADLAN
HHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCCEEEHHHHHHHCCCCCCCCCCCCCHHHH
VFALRTLEDTRRIAAALAAAQRVVVIGGGFIGLEFAAVAAKQGKQVTVLEAAGRLMARVV
HHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHH
APPVSDFYAALHRGHGVAIELGAAVSALRGKAGPAQDAVAAVCTADGREFPADLVIVGIG
CCCHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCHHHHEEEEECCCCCCCCEEEEEEEE
ILPNSELAEAAGLACERGIVVDGCSRTADPRIVASGDCTARRLEDGSLLRLESVQNAVEQ
CCCCCHHHHHHCCHHCCCEEEECCCCCCCCCEEECCCCCCEECCCCCEEEHHHHHHHHHH
GKSAAAALLGKERRFDAAPWFWSDQYDVKLQMVGLSAGYDQVVSRGEVESQRFSVFYFRT
CHHHHHHHHCCCCCCCCCCCEECCCCEEEEEEEEECCCHHHHHCCCCCCCCEEEEEEEEC
GKLVAIDSINQPQVHMLGRKLLDKGNSLTAAQAADPAFDLAGAAA
CCEEEEECCCCCHHHHHHHHHHCCCCCEEEHHCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]