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The map label for this gene is ppsA2 [H]

Identifier: 119898777

GI number: 119898777

Start: 2747955

End: 2750558

Strand: Reverse

Name: ppsA2 [H]

Synonym: azo2486

Alternate gene names: 119898777

Gene position: 2750558-2747955 (Counterclockwise)

Preceding gene: 119898778

Following gene: 119898776

Centisome position: 62.85

GC content: 74.54

Gene sequence:

>2604_bases
ATGAGCGCCGTGCCGCTGCTGATGGATCTGCCCGACGCGGCCGGCCTGACCGCGCTCGCCGGCGGAAAAGGCGCCGGGCT
GGGGCTGCTGGCGCGCTACGGCCTGCCGGTGCCTGCCGGGGCGGTGCTGACCACCGCCGCCTACCACCGCGCCGCGGCGG
AAACCGGGCTGGCGCTGTTGCCGGATGGCAGCGATGGCGGCGCGCTCGGCGCCCGCCGCGCCGCGCTGCTGCGCGCGCCG
CTGCCGGACGCGGTGGCGTACGCGCTGGAGCAGGCGGTGCGCGAGCGCGGCTGGACGGACCTTCCGCTTGCGGTGCGCTC
GTCCGCCCCGCAGGAGGATTCCGGCAGCGCGTCCTTCGCCGGCATCCACCACAGCGTGCTCAACGTGGTCGGCGCGGCCG
CGCTGGCCGATGCGGTGCGCGAGGTGTGGGCTTCGTTATGGACGCCGCAGGCCGCCGCCTACCGGGCGCGTTTCGGTATC
CCGGAGGGCGAGGCCGCGATGGCGGTGGTGGTGATGCCGCTGCTGCCGGCACGGGCCTCCGGCGTTGCCTTCAGCTGCGA
CCCGGCGAGCGGACGCGAGGACCTTTATGTGATCGAGGCGGTGCACGGCCTGGGTGAAGCGCTGGTCGGCGGACTGGTGG
CGGGCGAGCGCACCGTGCTGCAGGAAGACCCGGTGAGCGGCGGGTTTGCCGTGCAGCGCCGCGACGGGGGCGGGCAGGCC
GTGCGCGTGCTCCCGGCGCAAGGCGGCGGCACCCGGCGCGAGCCCCTGACGCTGCAGGCTGCGCCGGTGCTGGACGACGC
ACGCGCCTGCGAGCTTGCGGCGCTGGTGCGCGACGCCGCCCATGCGCTCGATTTCGGCGCGCCCTGGTACGACCTGGAGT
GGGCCTGGGACGGCGCGAAGTTCTGGCTGCTGCAGGCGCGCCCGGTGACCGCGCGGCCGTGGCACACCTATCCCGCGCTC
GCGGGGCAAAACGCGATCTGGTCCAACGGCAACACCCGCGACGTGGTGCCGCTACCGATGCAGGCGATGGACTGGGCGGT
GAGCCGCGGGCTGGTCGACCTGTTGCTGGAGGCTGGCTACCGCAGCGCGGGCTTCCCGCTGCTCGCCGGGGCGCGTCGCG
CCCGCTTGTTCAACGGCCGGCTCTATCTCAACGCCGCGTTCATCCAGTGGGAAGGCTATGACGGGTTCGGGGTGAGCCCG
GCGGCGATGAACCGCCTGCTCGGCGGCCACCACCCGGAGATCCGCGTTACGCCGCGCGACTGGCGGGCACGCCTGAAGGG
CCTGGCCTACATGCTGCGCTACTTGGTGCGAACCCCGGCACTGCGCCGGCGCGGCCGCGAGCAGGCGCGGGCCGCGTTCG
ACATCTGCCGGCGCTGGCGGGCCGAGGACCTGCGCGCGCTGTCGGACGCCGAACTCGCGACCCGGCTTGCCTCCGCCTTT
CCGCATGTGCAGGCGCAGGATGGGCTGTTGCTGCTGCAGGGCTCGTCCGGCGGCAATCTGCACATGTTGCTGGAGCTGGT
GGAGCGCTACCTGCCGGGGCAGGGCCACAGCCTGGTCGCGGCGCTGATGGCCGGCGGCGAGGGCAGCGTCAGCGCGCGCC
AGGGGTACGAGCTGATGGCGATCGCGCGCCGCGCGATGGCGGACCCGCGGGTGGCGGCCTGGCTGCAGGCCGGGGCGCCC
TACGACTTCGCCGCGCTGCCGGCGGACGCACCGTTCCGCGCCGAGTTCGCGCGCTTTCTCGATACCTACGGTCACCGCAG
CGTGCACGAATCCTATGTGCGCTCGCCGCGCTGGCGCGAGGAGCCGGCCTATCTGCTCGACTCCCTGCCGGCGCTGGCGG
CGGTGGACCACGCGGCGCTGGTGCGCCGCCAGCGCGAAGCGGCCGCCGCCGCGTGGGCGCAGCTTGCCGCGGCGCTGCCG
CGCAGCCGCCGGGGCTGGGCGCGGCAGTTGCTGAAGGCCGCGCGCGACGAAACGCCGGATCGCGAGTTGGCGCGCAGCGC
CATGACGGCCTACGGTGAAGTGGCGCGCCTGCGCCTGCTCGAAGTCGGTCGCCGGATGCAGGCGGAGGGCATGCTCGAGC
GCCCCGACCATGCTTTCGACCTGCTGCCGTGGGAGATGGAGGCCGCGCTGCACCGCCGTCTTGCTCCCGCAGCCTTCCGC
GAACGGGTGGCCGAGCGCCGGCGCCGCTTTGCCGAATGGGAGCGGCATTGCGCGGCGGACGTGATCGAGGAGGGCAGCGC
CCCGCCGGCGCCGGTGGAAGGCGTCGACGCAAGCGCGGCGGCCGGCGGCTGGCGCGGCATCGCGGTCGGCACCGGGCATG
TGGAAGGGATCGCGCGGCTTATCGACCATCCGGCGGAAGGCGCGCGCCTGGGAGCGGGCGAAATCCTCGTCGCGCCCGCC
ACCGATCCCGGCTGGACGCCGCTCTTCCTGCGGGCGGGCGGCCTCGTGATGGAGACCGGCGGCTATCTGTCGCACGGGGC
GATCGTCGCGCGCGAATTCGGCATCCCCGCGGTGGTCAATCTGCCGGGCATCCGCAGCGTGCTGCAGGACGGCCAGCGCA
TCCGCGTGGATGGGCGCAGCGGACGGGTGGAGGTGTTGGCGTGA

Upstream 100 bases:

>100_bases
GCATGGTGGCGTGGGTACAGAAACCCGGTAGTTCGCTGGCGCAGGAGTTCGACACCATGCTCGACCTCTTCCTGACCGGC
GCGCTGGCGGAGGTGTCGCG

Downstream 100 bases:

>100_bases
AGACAGGGAGAGGGCCATGGAGATGAAGCGCATCAGTGCCGGCAAGCTGCGGGCGATCGGCTACGACGCGCGCCAGCGCG
TGCTGCGGGTCGAACTCGAC

Product: putative phosphoenolpyruvate synthase

Products: AMP; phosphoenolpyruvate; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 867; Mature: 866

Protein sequence:

>867_residues
MSAVPLLMDLPDAAGLTALAGGKGAGLGLLARYGLPVPAGAVLTTAAYHRAAAETGLALLPDGSDGGALGARRAALLRAP
LPDAVAYALEQAVRERGWTDLPLAVRSSAPQEDSGSASFAGIHHSVLNVVGAAALADAVREVWASLWTPQAAAYRARFGI
PEGEAAMAVVVMPLLPARASGVAFSCDPASGREDLYVIEAVHGLGEALVGGLVAGERTVLQEDPVSGGFAVQRRDGGGQA
VRVLPAQGGGTRREPLTLQAAPVLDDARACELAALVRDAAHALDFGAPWYDLEWAWDGAKFWLLQARPVTARPWHTYPAL
AGQNAIWSNGNTRDVVPLPMQAMDWAVSRGLVDLLLEAGYRSAGFPLLAGARRARLFNGRLYLNAAFIQWEGYDGFGVSP
AAMNRLLGGHHPEIRVTPRDWRARLKGLAYMLRYLVRTPALRRRGREQARAAFDICRRWRAEDLRALSDAELATRLASAF
PHVQAQDGLLLLQGSSGGNLHMLLELVERYLPGQGHSLVAALMAGGEGSVSARQGYELMAIARRAMADPRVAAWLQAGAP
YDFAALPADAPFRAEFARFLDTYGHRSVHESYVRSPRWREEPAYLLDSLPALAAVDHAALVRRQREAAAAAWAQLAAALP
RSRRGWARQLLKAARDETPDRELARSAMTAYGEVARLRLLEVGRRMQAEGMLERPDHAFDLLPWEMEAALHRRLAPAAFR
ERVAERRRRFAEWERHCAADVIEEGSAPPAPVEGVDASAAAGGWRGIAVGTGHVEGIARLIDHPAEGARLGAGEILVAPA
TDPGWTPLFLRAGGLVMETGGYLSHGAIVAREFGIPAVVNLPGIRSVLQDGQRIRVDGRSGRVEVLA

Sequences:

>Translated_867_residues
MSAVPLLMDLPDAAGLTALAGGKGAGLGLLARYGLPVPAGAVLTTAAYHRAAAETGLALLPDGSDGGALGARRAALLRAP
LPDAVAYALEQAVRERGWTDLPLAVRSSAPQEDSGSASFAGIHHSVLNVVGAAALADAVREVWASLWTPQAAAYRARFGI
PEGEAAMAVVVMPLLPARASGVAFSCDPASGREDLYVIEAVHGLGEALVGGLVAGERTVLQEDPVSGGFAVQRRDGGGQA
VRVLPAQGGGTRREPLTLQAAPVLDDARACELAALVRDAAHALDFGAPWYDLEWAWDGAKFWLLQARPVTARPWHTYPAL
AGQNAIWSNGNTRDVVPLPMQAMDWAVSRGLVDLLLEAGYRSAGFPLLAGARRARLFNGRLYLNAAFIQWEGYDGFGVSP
AAMNRLLGGHHPEIRVTPRDWRARLKGLAYMLRYLVRTPALRRRGREQARAAFDICRRWRAEDLRALSDAELATRLASAF
PHVQAQDGLLLLQGSSGGNLHMLLELVERYLPGQGHSLVAALMAGGEGSVSARQGYELMAIARRAMADPRVAAWLQAGAP
YDFAALPADAPFRAEFARFLDTYGHRSVHESYVRSPRWREEPAYLLDSLPALAAVDHAALVRRQREAAAAAWAQLAAALP
RSRRGWARQLLKAARDETPDRELARSAMTAYGEVARLRLLEVGRRMQAEGMLERPDHAFDLLPWEMEAALHRRLAPAAFR
ERVAERRRRFAEWERHCAADVIEEGSAPPAPVEGVDASAAAGGWRGIAVGTGHVEGIARLIDHPAEGARLGAGEILVAPA
TDPGWTPLFLRAGGLVMETGGYLSHGAIVAREFGIPAVVNLPGIRSVLQDGQRIRVDGRSGRVEVLA
>Mature_866_residues
SAVPLLMDLPDAAGLTALAGGKGAGLGLLARYGLPVPAGAVLTTAAYHRAAAETGLALLPDGSDGGALGARRAALLRAPL
PDAVAYALEQAVRERGWTDLPLAVRSSAPQEDSGSASFAGIHHSVLNVVGAAALADAVREVWASLWTPQAAAYRARFGIP
EGEAAMAVVVMPLLPARASGVAFSCDPASGREDLYVIEAVHGLGEALVGGLVAGERTVLQEDPVSGGFAVQRRDGGGQAV
RVLPAQGGGTRREPLTLQAAPVLDDARACELAALVRDAAHALDFGAPWYDLEWAWDGAKFWLLQARPVTARPWHTYPALA
GQNAIWSNGNTRDVVPLPMQAMDWAVSRGLVDLLLEAGYRSAGFPLLAGARRARLFNGRLYLNAAFIQWEGYDGFGVSPA
AMNRLLGGHHPEIRVTPRDWRARLKGLAYMLRYLVRTPALRRRGREQARAAFDICRRWRAEDLRALSDAELATRLASAFP
HVQAQDGLLLLQGSSGGNLHMLLELVERYLPGQGHSLVAALMAGGEGSVSARQGYELMAIARRAMADPRVAAWLQAGAPY
DFAALPADAPFRAEFARFLDTYGHRSVHESYVRSPRWREEPAYLLDSLPALAAVDHAALVRRQREAAAAAWAQLAAALPR
SRRGWARQLLKAARDETPDRELARSAMTAYGEVARLRLLEVGRRMQAEGMLERPDHAFDLLPWEMEAALHRRLAPAAFRE
RVAERRRRFAEWERHCAADVIEEGSAPPAPVEGVDASAAAGGWRGIAVGTGHVEGIARLIDHPAEGARLGAGEILVAPAT
DPGWTPLFLRAGGLVMETGGYLSHGAIVAREFGIPAVVNLPGIRSVLQDGQRIRVDGRSGRVEVLA

Specific function: ESSENTIAL STEP IN GLUCONEOGENESIS WHEN PYRUVATE AND LACTATE ARE USED AS A CARBON SOURCE. [C]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=323, Percent_Identity=31.8885448916409, Blast_Score=99, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17564524, Length=919, Percent_Identity=22.8509249183896, Blast_Score=151, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR002192 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]

EC number: 2.7.9.2

Molecular weight: Translated: 92855; Mature: 92724

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVPLLMDLPDAAGLTALAGGKGAGLGLLARYGLPVPAGAVLTTAAYHRAAAETGLALL
CCCCCCEEECCCCCCCEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEE
PDGSDGGALGARRAALLRAPLPDAVAYALEQAVRERGWTDLPLAVRSSAPQEDSGSASFA
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHH
GIHHSVLNVVGAAALADAVREVWASLWTPQAAAYRARFGIPEGEAAMAVVVMPLLPARAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCC
GVAFSCDPASGREDLYVIEAVHGLGEALVGGLVAGERTVLQEDPVSGGFAVQRRDGGGQA
CCEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEECCCCCE
VRVLPAQGGGTRREPLTLQAAPVLDDARACELAALVRDAAHALDFGAPWYDLEWAWDGAK
EEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCE
FWLLQARPVTARPWHTYPALAGQNAIWSNGNTRDVVPLPMQAMDWAVSRGLVDLLLEAGY
EEEEECCCCCCCCCCCCCCCCCCCCEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCC
RSAGFPLLAGARRARLFNGRLYLNAAFIQWEGYDGFGVSPAAMNRLLGGHHPEIRVTPRD
CCCCCCHHHCCHHHHEECCEEEEEEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEECCHH
WRARLKGLAYMLRYLVRTPALRRRGREQARAAFDICRRWRAEDLRALSDAELATRLASAF
HHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PHVQAQDGLLLLQGSSGGNLHMLLELVERYLPGQGHSLVAALMAGGEGSVSARQGYELMA
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHCCHHHHH
IARRAMADPRVAAWLQAGAPYDFAALPADAPFRAEFARFLDTYGHRSVHESYVRSPRWRE
HHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCC
EPAYLLDSLPALAAVDHAALVRRQREAAAAAWAQLAAALPRSRRGWARQLLKAARDETPD
CHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCH
RELARSAMTAYGEVARLRLLEVGRRMQAEGMLERPDHAFDLLPWEMEAALHRRLAPAAFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHCCHHHHH
ERVAERRRRFAEWERHCAADVIEEGSAPPAPVEGVDASAAAGGWRGIAVGTGHVEGIARL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHH
IDHPAEGARLGAGEILVAPATDPGWTPLFLRAGGLVMETGGYLSHGAIVAREFGIPAVVN
HCCCCCCCEECCCCEEEECCCCCCCCCCEEECCCEEEECCCCCCCCEEEEHHCCCCEEEE
LPGIRSVLQDGQRIRVDGRSGRVEVLA
CCHHHHHHCCCCEEEEECCCCCEEEEC
>Mature Secondary Structure 
SAVPLLMDLPDAAGLTALAGGKGAGLGLLARYGLPVPAGAVLTTAAYHRAAAETGLALL
CCCCCEEECCCCCCCEEECCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCEEEE
PDGSDGGALGARRAALLRAPLPDAVAYALEQAVRERGWTDLPLAVRSSAPQEDSGSASFA
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCHHH
GIHHSVLNVVGAAALADAVREVWASLWTPQAAAYRARFGIPEGEAAMAVVVMPLLPARAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCC
GVAFSCDPASGREDLYVIEAVHGLGEALVGGLVAGERTVLQEDPVSGGFAVQRRDGGGQA
CCEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEEEEECCCCCE
VRVLPAQGGGTRREPLTLQAAPVLDDARACELAALVRDAAHALDFGAPWYDLEWAWDGAK
EEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCE
FWLLQARPVTARPWHTYPALAGQNAIWSNGNTRDVVPLPMQAMDWAVSRGLVDLLLEAGY
EEEEECCCCCCCCCCCCCCCCCCCCEECCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCC
RSAGFPLLAGARRARLFNGRLYLNAAFIQWEGYDGFGVSPAAMNRLLGGHHPEIRVTPRD
CCCCCCHHHCCHHHHEECCEEEEEEEEEEEECCCCCCCCHHHHHHHHCCCCCEEEECCHH
WRARLKGLAYMLRYLVRTPALRRRGREQARAAFDICRRWRAEDLRALSDAELATRLASAF
HHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PHVQAQDGLLLLQGSSGGNLHMLLELVERYLPGQGHSLVAALMAGGEGSVSARQGYELMA
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHCCHHHHH
IARRAMADPRVAAWLQAGAPYDFAALPADAPFRAEFARFLDTYGHRSVHESYVRSPRWRE
HHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCC
EPAYLLDSLPALAAVDHAALVRRQREAAAAAWAQLAAALPRSRRGWARQLLKAARDETPD
CHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCH
RELARSAMTAYGEVARLRLLEVGRRMQAEGMLERPDHAFDLLPWEMEAALHRRLAPAAFR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHCCHHHHH
ERVAERRRRFAEWERHCAADVIEEGSAPPAPVEGVDASAAAGGWRGIAVGTGHVEGIARL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHH
IDHPAEGARLGAGEILVAPATDPGWTPLFLRAGGLVMETGGYLSHGAIVAREFGIPAVVN
HCCCCCCCEECCCCEEEECCCCCCCCCCEEECCCEEEECCCCCCCCEEEEHHCCCCEEEE
LPGIRSVLQDGQRIRVDGRSGRVEVLA
CCHHHHHHCCCCEEEEECCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10.5 {phosphate}} 0.028 {ATP}} 0.083 {pyruvate}} [C]

Substrates: ATP; pyruvate; H2O

Specific reaction: ATP + pyruvate + H2O = AMP + phosphoenolpyruvate + phosphate

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]