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The map label for this gene is rpfG [H]

Identifier: 119898775

GI number: 119898775

Start: 2746361

End: 2747515

Strand: Reverse

Name: rpfG [H]

Synonym: azo2484

Alternate gene names: 119898775

Gene position: 2747515-2746361 (Counterclockwise)

Preceding gene: 119898776

Following gene: 119898774

Centisome position: 62.79

GC content: 66.93

Gene sequence:

>1155_bases
GTGGGTGAACCGCTCCGGCAGGCGGCTGCGACACCGCCCCGCCGTACCCTCCTGGTCGTAGATGACACGCCGCAGAATCT
CGCGGTGCTCGGCGAAGTGCTGAAATCGCACTATCAGGTGCGTGCAGTCAATTCGGGCGAACGCGCGCTTCGTGCCGCCC
ATACCGAGCCGCGTCCGGACCTCATCCTGCTCGACGTGATGATGCCGGAGATGGACGGCTACGAAGTGCTGCGCCATCTG
CGCGAAGACCCTGCCACCCGCGCCACGCCGGTGATCTTCATCACCGCGATGACCGGCTCCGACGACGAGATGCGCGGGCT
GGAACTGGGCGCGGTCGATTACATCACCAAGCCGTTCAATCCCTCCATCGTGCTGGCGCGCGTGCGCACCCAGCTGGAAC
TGAAGGAGGCCCGCGACCGCCTGGCGCGCGAGAACGAGTGGCTGGAGGCCGAAGTGCACCGGCGCATGCGCGAGAACCTG
CTGATCCAGGATCTCAACGTGCGTGCGCTCGCCTGTTTGGCCGAGGCGCGCGACAACGAAACCGGCAACCACATCATCCG
CACGCAGTTCTACGTAAAGGTGCTCGCCACCCGGCTGCTGCCCAATCCGCGCTTCACCGCGGCGCTGTCCGGCGGACGGC
TGGACATCACCGTGAAGGCGGCGCCCCTGCACGACATCGGCAAGGTCGGCATTCCGGACGCGATCCTGCTCAAGCCCGGC
CGGCTCACGCCGGAAGAGTTCGAGATCATGAAGACGCACCCGGTCATCGGCGCGGAGGCGATCGAGCGCGCGATGGACCA
GGCCCTGGGCGGGGCGGACCGCGACGCGGCCTCGCTGGCCGGCGGTGCGTTCGCGTTTCTCGAGATCGCGCGCGAAATCT
CGCTCGGCCACCACGAGAAATGGGACGGCAGCGGCTACCCGGCCGGGCTCGCGGGCGATGCGATTCCGGTGTCGGCCCGG
CTGATGGCGCTGGCCGACGTGTTCGACGCGCTGATCAGCCGTCGCGTCTACAAGGCACCGATGAGCCTCGACGAGGCGAC
CCGCATCATCGTCGAAGGCCGCGGCCGCCATTTCGACCCCGAGATCGTCGACGCCTTTCTCGAATGCAAGGATCAATTTG
CCGAAATCGCCGCCCGCTATGTCGATGAAGAGTAA

Upstream 100 bases:

>100_bases
CGCATCGCCAGAATCGCTGCTTTCGTGCCGCAGTGCTGGCAATCCCACGTGGCCGGCAGGGTATGATTACCCGGGCTGAG
CCATAACAAGGAATACCTCC

Downstream 100 bases:

>100_bases
GTTCCGCCTGTTTCACGCCGCGCTGGCGGCGACCCTCGCGCTGAGCGGCGCGCTGCCGCTTGCCGTGCGCGCCGGCGACA
TGCCCGCACCCGCGGCGCCG

Product: putative response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MGEPLRQAAATPPRRTLLVVDDTPQNLAVLGEVLKSHYQVRAVNSGERALRAAHTEPRPDLILLDVMMPEMDGYEVLRHL
REDPATRATPVIFITAMTGSDDEMRGLELGAVDYITKPFNPSIVLARVRTQLELKEARDRLARENEWLEAEVHRRMRENL
LIQDLNVRALACLAEARDNETGNHIIRTQFYVKVLATRLLPNPRFTAALSGGRLDITVKAAPLHDIGKVGIPDAILLKPG
RLTPEEFEIMKTHPVIGAEAIERAMDQALGGADRDAASLAGGAFAFLEIAREISLGHHEKWDGSGYPAGLAGDAIPVSAR
LMALADVFDALISRRVYKAPMSLDEATRIIVEGRGRHFDPEIVDAFLECKDQFAEIAARYVDEE

Sequences:

>Translated_384_residues
MGEPLRQAAATPPRRTLLVVDDTPQNLAVLGEVLKSHYQVRAVNSGERALRAAHTEPRPDLILLDVMMPEMDGYEVLRHL
REDPATRATPVIFITAMTGSDDEMRGLELGAVDYITKPFNPSIVLARVRTQLELKEARDRLARENEWLEAEVHRRMRENL
LIQDLNVRALACLAEARDNETGNHIIRTQFYVKVLATRLLPNPRFTAALSGGRLDITVKAAPLHDIGKVGIPDAILLKPG
RLTPEEFEIMKTHPVIGAEAIERAMDQALGGADRDAASLAGGAFAFLEIAREISLGHHEKWDGSGYPAGLAGDAIPVSAR
LMALADVFDALISRRVYKAPMSLDEATRIIVEGRGRHFDPEIVDAFLECKDQFAEIAARYVDEE
>Mature_383_residues
GEPLRQAAATPPRRTLLVVDDTPQNLAVLGEVLKSHYQVRAVNSGERALRAAHTEPRPDLILLDVMMPEMDGYEVLRHLR
EDPATRATPVIFITAMTGSDDEMRGLELGAVDYITKPFNPSIVLARVRTQLELKEARDRLARENEWLEAEVHRRMRENLL
IQDLNVRALACLAEARDNETGNHIIRTQFYVKVLATRLLPNPRFTAALSGGRLDITVKAAPLHDIGKVGIPDAILLKPGR
LTPEEFEIMKTHPVIGAEAIERAMDQALGGADRDAASLAGGAFAFLEIAREISLGHHEKWDGSGYPAGLAGDAIPVSARL
MALADVFDALISRRVYKAPMSLDEATRIIVEGRGRHFDPEIVDAFLECKDQFAEIAARYVDEE

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=116, Percent_Identity=39.6551724137931, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=40.6779661016949, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788394, Length=117, Percent_Identity=37.6068376068376, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789809, Length=117, Percent_Identity=41.025641025641, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=36.7521367521368, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1789149, Length=107, Percent_Identity=38.3177570093458, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790860, Length=74, Percent_Identity=47.2972972972973, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788192, Length=106, Percent_Identity=35.8490566037736, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI2367329, Length=114, Percent_Identity=38.5964912280702, Blast_Score=62, Evalue=7e-11,
Organism=Escherichia coli, GI1788550, Length=126, Percent_Identity=31.7460317460317, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 42467; Mature: 42336

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEPLRQAAATPPRRTLLVVDDTPQNLAVLGEVLKSHYQVRAVNSGERALRAAHTEPRPD
CCCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHCCCCCCC
LILLDVMMPEMDGYEVLRHLREDPATRATPVIFITAMTGSDDEMRGLELGAVDYITKPFN
EEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHCCCEECCHHHHCCCCC
PSIVLARVRTQLELKEARDRLARENEWLEAEVHRRMRENLLIQDLNVRALACLAEARDNE
CCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
TGNHIIRTQFYVKVLATRLLPNPRFTAALSGGRLDITVKAAPLHDIGKVGIPDAILLKPG
CCCEEEHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEECCCHHCCCCCCCCEEEECCC
RLTPEEFEIMKTHPVIGAEAIERAMDQALGGADRDAASLAGGAFAFLEIAREISLGHHEK
CCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCC
WDGSGYPAGLAGDAIPVSARLMALADVFDALISRRVYKAPMSLDEATRIIVEGRGRHFDP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEECCCCCCCH
EIVDAFLECKDQFAEIAARYVDEE
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GEPLRQAAATPPRRTLLVVDDTPQNLAVLGEVLKSHYQVRAVNSGERALRAAHTEPRPD
CCHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCEEEEEECCCHHHHHHHCCCCCCC
LILLDVMMPEMDGYEVLRHLREDPATRATPVIFITAMTGSDDEMRGLELGAVDYITKPFN
EEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHCCCEECCHHHHCCCCC
PSIVLARVRTQLELKEARDRLARENEWLEAEVHRRMRENLLIQDLNVRALACLAEARDNE
CCEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
TGNHIIRTQFYVKVLATRLLPNPRFTAALSGGRLDITVKAAPLHDIGKVGIPDAILLKPG
CCCEEEHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEEECCCHHCCCCCCCCEEEECCC
RLTPEEFEIMKTHPVIGAEAIERAMDQALGGADRDAASLAGGAFAFLEIAREISLGHHEK
CCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCC
WDGSGYPAGLAGDAIPVSARLMALADVFDALISRRVYKAPMSLDEATRIIVEGRGRHFDP
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEEEECCCCCCCH
EIVDAFLECKDQFAEIAARYVDEE
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]