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The map label for this gene is nahM

Identifier: 119898722

GI number: 119898722

Start: 2683840

End: 2684880

Strand: Reverse

Name: nahM

Synonym: azo2431

Alternate gene names: 119898722

Gene position: 2684880-2683840 (Counterclockwise)

Preceding gene: 119898723

Following gene: 119898721

Centisome position: 61.35

GC content: 65.61

Gene sequence:

>1041_bases
ATGGACCTTCGCGGCACACGCATCACCGTTCACGACATGACCCTGCGCGACGGCATGCACCCCAAGCGCCACCTGATGAC
GCTCGAGCAGATGAAGACCATCGCTCAGGGGCTCGACCAAGCGGGCATCCCGCTGATCGAAGTCACCCACGGCGACGGCC
TGGGCGGCAGCTCGGTGAACTACGGCTTTCCGGCCCACACCGACGAGGAATACCTCGGCGCGGTGATTCCGCTGATGAAG
CAGGCCAAGGTCTCGGCACTGCTGCTGCCGGGCATCGGCACCGTCGATCACCTGAAGATGGCGCATGAACTCGGGGTGAA
CACCATCCGTGTGGCCACCCACTGCACCGAAGCCGATGTCTCCGAGCAGCACATCCGCTACGCGAGAAAGCTCGGCATGG
ACACCGTGGGCTTTCTGATGATGGCCCACATGAACAGCCCCGAAGGACTGGTGCAGCAGGCGAAGCTGATGGAGAGCTAT
GGCGCCAACTGCATCTACGTGACGGACTCGGCCGGGCACCTGCTGCCCGACACGGTGAAGGCGCGCATCGGCGCGGTCCG
TGCGGCCTTGAAGCCCGAAACCGAACTCGGTTTCCACGGCCACCACAACCTCGCGATGGGCGTTGCCAACTCCATCGCCG
CGATCGAAGCCGGGGCGACCCGCATCGACGCCGCCGCGGCCGGACTCGGCGCGGGCGCCGGCAACACGCCGATGGAAGTG
CTGATCGCGGTGTGCGACCTGATGGGGATCGAGACCGGGGTCGACGTGTTCAAGATCCAGGACGTCGCCGAAGACCTCGT
GGTGCCCATCATGGACTTCCCGATCCGCATCGACCGCGATGCGCTCACGCTGGGCTACGCCGGCGTCTATGGCTCCTTCC
TGCTCTTTGCCAAGCGCGCCGAGAAGAAGTACGGCGTGCCGGCGCGCGAGATCCTGGTCGAGATGGGCCGGCGCGGCATG
GTCGGCGGCCAGGAGGACATGATCGAGGACACCGCGATCACGCTGGCGAAGGCGAAACAGGCACAAGGAGTCGCAGCATG
A

Upstream 100 bases:

>100_bases
CCGGTGCCCGCACCGCCGAGATGTTCGCCGAGGAAATCCTCGCCGGCCGCCTCACCCTCGAATCCAACCGCGCCGTGCTG
GCTTGAATCAGGAGAAAACC

Downstream 100 bases:

>100_bases
AACTGTCGCGCGAAATCATCGAACAACTCGCCATCCGCCTCGAAACCTGCGAGCTGGAAGCGAAGGACACCACCAAGATC
ACCGACGACCATCCCGACAT

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA 3; 4-hydroxy-2-keto-pentanoic acid aldolase 3; 4-hydroxy-2-oxopentanoate aldolase 3

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MDLRGTRITVHDMTLRDGMHPKRHLMTLEQMKTIAQGLDQAGIPLIEVTHGDGLGGSSVNYGFPAHTDEEYLGAVIPLMK
QAKVSALLLPGIGTVDHLKMAHELGVNTIRVATHCTEADVSEQHIRYARKLGMDTVGFLMMAHMNSPEGLVQQAKLMESY
GANCIYVTDSAGHLLPDTVKARIGAVRAALKPETELGFHGHHNLAMGVANSIAAIEAGATRIDAAAAGLGAGAGNTPMEV
LIAVCDLMGIETGVDVFKIQDVAEDLVVPIMDFPIRIDRDALTLGYAGVYGSFLLFAKRAEKKYGVPAREILVEMGRRGM
VGGQEDMIEDTAITLAKAKQAQGVAA

Sequences:

>Translated_346_residues
MDLRGTRITVHDMTLRDGMHPKRHLMTLEQMKTIAQGLDQAGIPLIEVTHGDGLGGSSVNYGFPAHTDEEYLGAVIPLMK
QAKVSALLLPGIGTVDHLKMAHELGVNTIRVATHCTEADVSEQHIRYARKLGMDTVGFLMMAHMNSPEGLVQQAKLMESY
GANCIYVTDSAGHLLPDTVKARIGAVRAALKPETELGFHGHHNLAMGVANSIAAIEAGATRIDAAAAGLGAGAGNTPMEV
LIAVCDLMGIETGVDVFKIQDVAEDLVVPIMDFPIRIDRDALTLGYAGVYGSFLLFAKRAEKKYGVPAREILVEMGRRGM
VGGQEDMIEDTAITLAKAKQAQGVAA
>Mature_346_residues
MDLRGTRITVHDMTLRDGMHPKRHLMTLEQMKTIAQGLDQAGIPLIEVTHGDGLGGSSVNYGFPAHTDEEYLGAVIPLMK
QAKVSALLLPGIGTVDHLKMAHELGVNTIRVATHCTEADVSEQHIRYARKLGMDTVGFLMMAHMNSPEGLVQQAKLMESY
GANCIYVTDSAGHLLPDTVKARIGAVRAALKPETELGFHGHHNLAMGVANSIAAIEAGATRIDAAAAGLGAGAGNTPMEV
LIAVCDLMGIETGVDVFKIQDVAEDLVVPIMDFPIRIDRDALTLGYAGVYGSFLLFAKRAEKKYGVPAREILVEMGRRGM
VGGQEDMIEDTAITLAKAKQAQGVAA

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Escherichia coli, GI1786548, Length=337, Percent_Identity=57.2700296735905, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1786261, Length=295, Percent_Identity=25.7627118644068, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA3_AZOSB (A1K893)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_933935.1
- ProteinModelPortal:   A1K893
- SMR:   A1K893
- STRING:   A1K893
- GeneID:   4609320
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo2431
- eggNOG:   COG0119
- HOGENOM:   HBG300122
- OMA:   SEQHIRY
- ProtClustDB:   PRK08195
- BioCyc:   ASP62928:AZO2431-MONOMER
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 36902; Mature: 36902

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 170-170 BINDING 199-199 BINDING 290-290

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRGTRITVHDMTLRDGMHPKRHLMTLEQMKTIAQGLDQAGIPLIEVTHGDGLGGSSVN
CCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
YGFPAHTDEEYLGAVIPLMKQAKVSALLLPGIGTVDHLKMAHELGVNTIRVATHCTEADV
CCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCC
SEQHIRYARKLGMDTVGFLMMAHMNSPEGLVQQAKLMESYGANCIYVTDSAGHLLPDTVK
HHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHH
ARIGAVRAALKPETELGFHGHHNLAMGVANSIAAIEAGATRIDAAAAGLGAGAGNTPMEV
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHH
LIAVCDLMGIETGVDVFKIQDVAEDLVVPIMDFPIRIDRDALTLGYAGVYGSFLLFAKRA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHEECHHHHHHHHHHHHHHH
EKKYGVPAREILVEMGRRGMVGGQEDMIEDTAITLAKAKQAQGVAA
HHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDLRGTRITVHDMTLRDGMHPKRHLMTLEQMKTIAQGLDQAGIPLIEVTHGDGLGGSSVN
CCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCC
YGFPAHTDEEYLGAVIPLMKQAKVSALLLPGIGTVDHLKMAHELGVNTIRVATHCTEADV
CCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCC
SEQHIRYARKLGMDTVGFLMMAHMNSPEGLVQQAKLMESYGANCIYVTDSAGHLLPDTVK
HHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHH
ARIGAVRAALKPETELGFHGHHNLAMGVANSIAAIEAGATRIDAAAAGLGAGAGNTPMEV
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHH
LIAVCDLMGIETGVDVFKIQDVAEDLVVPIMDFPIRIDRDALTLGYAGVYGSFLLFAKRA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCHHEECHHHHHHHHHHHHHHH
EKKYGVPAREILVEMGRRGMVGGQEDMIEDTAITLAKAKQAQGVAA
HHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA