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The map label for this gene is dctP3 [H]

Identifier: 119898715

GI number: 119898715

Start: 2677699

End: 2678685

Strand: Direct

Name: dctP3 [H]

Synonym: azo2424

Alternate gene names: 119898715

Gene position: 2677699-2678685 (Clockwise)

Preceding gene: 119898714

Following gene: 119898716

Centisome position: 61.19

GC content: 67.27

Gene sequence:

>987_bases
ATGAGAAAGAGCCTGATCACCACCCTCAAGACCGCCGCGGCCGCCGGCCTGTGCGCACTTGCTTTCGGCGCCGGCAGCGC
CTCGGCGCAGGCGCTCAAGCTGGGCCACATCACCCCGCCCAGCCACGTCTGGCATCAGGTGTCGGAAAAGATCGCCGCCG
ACCTCGAAAAAGCCTCCGGCGGCAAGATGAAGACCGCGGTCAGCCCGCTGCAGAAGCTGGGCAACGAGGCCCAGATGATC
AACCTGATGCAGGCCGGCGCGATGCAGTTCGGCGTGTTCACCGTGGGCGGCCTGTCCAACCGCGAGGAGTCCTTCCTGGC
CTGGTCGCTGCCCTACACTTTCCGCGACGTCGAGCACGCCACCCGCGCCGCGGCCTCGCCCGCCGCGCAGGAAATGCTGA
AGCGGCTGGAGGCGCACGGCATGGTCGGCCTCGGCTACGCCTTCGCCGGCATGCGCCACGTGCTGTCGCTGGAGCCGGTG
AAGTCGCCCAAGGACCTCGCCAACAAGAAGATCCGCTCCTTCCCCAGCCCGATCTACAACGACTGGTGGACCGCCAACGG
CGCCGCCCCCACCGCGCTGCCGCTGTTCGAAGTGGCGCCCTCGCTGACCACCAAGCTGCTCGACGCGGTGGACGTGGACC
TCGATGCGCTGGTCGGACTCAAGTTCCACCAGCAGGCGCCCAACCTCACGCTCACCAACCACATGGCCTTCCCGGCCGCG
ATCGTGGTGTCGAAGAAGTGGTGGGATGCGCGCACCGATGCCGAGCGCGCCATGATCCGCAAGGTGGTCGCCGACGCCGA
AACCTGGGGCTACAAGACCGCGATCGAGGCCGACAAGAGCAATCTCGAGAAGGCCAAGGCCGACGGCGCCAAGGTGATCG
AAGCCGACCTCAAGGCCTTCCAGGCGGTGGGCGGCCCGGTGCGCGACAAGTACACCGGCAAGAACCCGCTGATCGCCGAC
TTCTACAAGCAGTCGCAGGGGCTGTAA

Upstream 100 bases:

>100_bases
CGCGCGCCAGCCCGACGCACCCGCCTGAGCCGGGCCGGCAGCCCCGATTTCCAACAAGCATGCGCCGCATGCAAAAACCT
CACATCCAGGAGGAGATACG

Downstream 100 bases:

>100_bases
GCGCTGCGGCGGTGATCTCCCGGCCTGACCCATCCCCACCCCCACCCTCCCCTTGAAGGGGAGGGAGCAAAACGGGCCAA
CCCGGGAGCCTTCGCTGCGC

Product: putative C4-dicarboxylate-binding periplasmic protein

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MRKSLITTLKTAAAAGLCALAFGAGSASAQALKLGHITPPSHVWHQVSEKIAADLEKASGGKMKTAVSPLQKLGNEAQMI
NLMQAGAMQFGVFTVGGLSNREESFLAWSLPYTFRDVEHATRAAASPAAQEMLKRLEAHGMVGLGYAFAGMRHVLSLEPV
KSPKDLANKKIRSFPSPIYNDWWTANGAAPTALPLFEVAPSLTTKLLDAVDVDLDALVGLKFHQQAPNLTLTNHMAFPAA
IVVSKKWWDARTDAERAMIRKVVADAETWGYKTAIEADKSNLEKAKADGAKVIEADLKAFQAVGGPVRDKYTGKNPLIAD
FYKQSQGL

Sequences:

>Translated_328_residues
MRKSLITTLKTAAAAGLCALAFGAGSASAQALKLGHITPPSHVWHQVSEKIAADLEKASGGKMKTAVSPLQKLGNEAQMI
NLMQAGAMQFGVFTVGGLSNREESFLAWSLPYTFRDVEHATRAAASPAAQEMLKRLEAHGMVGLGYAFAGMRHVLSLEPV
KSPKDLANKKIRSFPSPIYNDWWTANGAAPTALPLFEVAPSLTTKLLDAVDVDLDALVGLKFHQQAPNLTLTNHMAFPAA
IVVSKKWWDARTDAERAMIRKVVADAETWGYKTAIEADKSNLEKAKADGAKVIEADLKAFQAVGGPVRDKYTGKNPLIAD
FYKQSQGL
>Mature_328_residues
MRKSLITTLKTAAAAGLCALAFGAGSASAQALKLGHITPPSHVWHQVSEKIAADLEKASGGKMKTAVSPLQKLGNEAQMI
NLMQAGAMQFGVFTVGGLSNREESFLAWSLPYTFRDVEHATRAAASPAAQEMLKRLEAHGMVGLGYAFAGMRHVLSLEPV
KSPKDLANKKIRSFPSPIYNDWWTANGAAPTALPLFEVAPSLTTKLLDAVDVDLDALVGLKFHQQAPNLTLTNHMAFPAA
IVVSKKWWDARTDAERAMIRKVVADAETWGYKTAIEADKSNLEKAKADGAKVIEADLKAFQAVGGPVRDKYTGKNPLIAD
FYKQSQGL

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=312, Percent_Identity=28.525641025641, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 35284; Mature: 35284

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSLITTLKTAAAAGLCALAFGAGSASAQALKLGHITPPSHVWHQVSEKIAADLEKASG
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCCHHHHHHHHHHHHHHHHHHCCC
GKMKTAVSPLQKLGNEAQMINLMQAGAMQFGVFTVGGLSNREESFLAWSLPYTFRDVEHA
CCHHHHHHHHHHHCCHHHHHHHHHHCCHHCCEEEECCCCCCCCCEEEEECCCHHHHHHHH
TRAAASPAAQEMLKRLEAHGMVGLGYAFAGMRHVLSLEPVKSPKDLANKKIRSFPSPIYN
HHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHC
DWWTANGAAPTALPLFEVAPSLTTKLLDAVDVDLDALVGLKFHQQAPNLTLTNHMAFPAA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCCCCCEEECCCCCCCC
IVVSKKWWDARTDAERAMIRKVVADAETWGYKTAIEADKSNLEKAKADGAKVIEADLKAF
EEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHCCCHHHHHHHHHHH
QAVGGPVRDKYTGKNPLIADFYKQSQGL
HHHCCCCCCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MRKSLITTLKTAAAAGLCALAFGAGSASAQALKLGHITPPSHVWHQVSEKIAADLEKASG
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEECCCCCHHHHHHHHHHHHHHHHHHCCC
GKMKTAVSPLQKLGNEAQMINLMQAGAMQFGVFTVGGLSNREESFLAWSLPYTFRDVEHA
CCHHHHHHHHHHHCCHHHHHHHHHHCCHHCCEEEECCCCCCCCCEEEEECCCHHHHHHHH
TRAAASPAAQEMLKRLEAHGMVGLGYAFAGMRHVLSLEPVKSPKDLANKKIRSFPSPIYN
HHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHC
DWWTANGAAPTALPLFEVAPSLTTKLLDAVDVDLDALVGLKFHQQAPNLTLTNHMAFPAA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCCCCCEEECCCCCCCC
IVVSKKWWDARTDAERAMIRKVVADAETWGYKTAIEADKSNLEKAKADGAKVIEADLKAF
EEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHCCCHHHHHHHHHHH
QAVGGPVRDKYTGKNPLIADFYKQSQGL
HHHCCCCCCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]