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The map label for this gene is phaE [H]

Identifier: 119898686

GI number: 119898686

Start: 2633703

End: 2635040

Strand: Direct

Name: phaE [H]

Synonym: azo2395

Alternate gene names: 119898686

Gene position: 2633703-2635040 (Clockwise)

Preceding gene: 119898685

Following gene: 119898687

Centisome position: 60.18

GC content: 67.64

Gene sequence:

>1338_bases
ATGCCCCGCGACACCAACCAGAAATACTGGGACCCGCAACTCGAAACCCAGAGCCGCGCCGAATGGGACGCGCTCAAGCT
CGGTCTGCTGAAGGACCACCTGAAGCACGCCTACGACAATTCGCCCTATTACCGCAGCAGCTTCGACGCTGCGGGCGTGA
AGCCGGAAGACCTGAAGACACTCGCCGACCTGCAGCGCTTCCCGACCATCGACAAGAAAATCCTGCGCGAACGCCAGGAG
GCGAAACCGCTGCTCGGCGACCTCGCCGCGGTGCCGGAACGCGACGTGGTGTACGTGTCGGCTTCGTCCGGCTCTACCGG
CGTACCCACGGTGTCGCCCTTCACCCAGCAGGATTTCGACGACTGGATCGACTACGAGGCGCGCCAGTTCTGGTCCTCCG
GCCTGCGGCCTGAAGACCGCTACGCGCATTCGCTCAACTTCTCGCTGTTCATCGGCGGCCCCTGCGTGCTCGGCGCGCAG
AAGCTCGGCGCGCTCAGCATCCACGCCGGCACGGTGCCGTCCGAACGCCTGCTGGCGATCCTGCGCCAGTTCCAGGCCAC
CGCGATCTGGACCACGCCGTCCTACGCCTGGTACCTGGGCGAGACCGCGCAGAAGGAAGGCATCGACCCGAAAAAGGATC
TGGCAGTGAAGCGCATCTTCGTCGCCGGCGAGCCGGGCGGGTCGATTCCCGAGACCCGCGCCCGCATCGAGGCGCTGTGG
GGCGCCGAGGTGTACGACTACTACGGCCTGTCGGACATCTTCGGCTCCTGCGCCGGCATGTGCGAGGAAAAGGACGGCCT
GCACTGGGCGGAAGACCACATCCTGGTCGAAGTGCTCGATCCGGAAACCGGCGAGGCGGTCGCCCCCGGCGAACGCGGCG
AGATGGTGCTGACCACGCTGAAGAAGCGTGCCCGCCCGCTGATCCGCTTCCGCACCGGCGACATCGTCAGCTTCACCGAC
GAGCCCTGCGCCTGCGGCCGCACCAGCCAGCGCCTGCTCGGCGTGCACGGCCGGCGCGACGACATGCTCATCGTGCGCGG
CGTGAACATCTTCCCGAGCGACGTCGAAGCCATCGTGCGCAAGGACCACGACTTCAGCGGCGAATACCGGCTGGTGATCG
AGCGCATCGACCATCTCGACCGCCTCACCATCGAGGTCGAACGCGCCGCCGGCTTCCAAGGCACCGCCGACGCACTCGCC
GACCGCCTCGCCCATCGCGTCAAGGCGATCACCGGCGTCGGCGCCCACATCGCGGTGCTCGACCCCGACACCCTGCCGCG
CGCCACCCACAAGGCCAAACGGGTGGAAGACCGCCGCAGCCAGGTGTGGACCGCGTAG

Upstream 100 bases:

>100_bases
GCCGTTCGCCGCTTGGAAGCCCGCCGCACGCAAGGGCGGCTGAGCCTTGCCCGCCCCGTCCGCACCCGCCCGCCCCTCCC
GCGCCCGAACAGGAACCGCC

Downstream 100 bases:

>100_bases
AAGCGCGGCCTTGCGCGATCCGCTCATCCAACCGATGCCATTCGAGGACCACCCATAGTGGTCCGGCGCGCGCGCACCCA
CTACATCCAGTGATGAAGGT

Product: phenylacetate--CoA ligase

Products: NA

Alternate protein names: Phenylacetyl-CoA ligase; PA-CoA ligase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MPRDTNQKYWDPQLETQSRAEWDALKLGLLKDHLKHAYDNSPYYRSSFDAAGVKPEDLKTLADLQRFPTIDKKILRERQE
AKPLLGDLAAVPERDVVYVSASSGSTGVPTVSPFTQQDFDDWIDYEARQFWSSGLRPEDRYAHSLNFSLFIGGPCVLGAQ
KLGALSIHAGTVPSERLLAILRQFQATAIWTTPSYAWYLGETAQKEGIDPKKDLAVKRIFVAGEPGGSIPETRARIEALW
GAEVYDYYGLSDIFGSCAGMCEEKDGLHWAEDHILVEVLDPETGEAVAPGERGEMVLTTLKKRARPLIRFRTGDIVSFTD
EPCACGRTSQRLLGVHGRRDDMLIVRGVNIFPSDVEAIVRKDHDFSGEYRLVIERIDHLDRLTIEVERAAGFQGTADALA
DRLAHRVKAITGVGAHIAVLDPDTLPRATHKAKRVEDRRSQVWTA

Sequences:

>Translated_445_residues
MPRDTNQKYWDPQLETQSRAEWDALKLGLLKDHLKHAYDNSPYYRSSFDAAGVKPEDLKTLADLQRFPTIDKKILRERQE
AKPLLGDLAAVPERDVVYVSASSGSTGVPTVSPFTQQDFDDWIDYEARQFWSSGLRPEDRYAHSLNFSLFIGGPCVLGAQ
KLGALSIHAGTVPSERLLAILRQFQATAIWTTPSYAWYLGETAQKEGIDPKKDLAVKRIFVAGEPGGSIPETRARIEALW
GAEVYDYYGLSDIFGSCAGMCEEKDGLHWAEDHILVEVLDPETGEAVAPGERGEMVLTTLKKRARPLIRFRTGDIVSFTD
EPCACGRTSQRLLGVHGRRDDMLIVRGVNIFPSDVEAIVRKDHDFSGEYRLVIERIDHLDRLTIEVERAAGFQGTADALA
DRLAHRVKAITGVGAHIAVLDPDTLPRATHKAKRVEDRRSQVWTA
>Mature_444_residues
PRDTNQKYWDPQLETQSRAEWDALKLGLLKDHLKHAYDNSPYYRSSFDAAGVKPEDLKTLADLQRFPTIDKKILRERQEA
KPLLGDLAAVPERDVVYVSASSGSTGVPTVSPFTQQDFDDWIDYEARQFWSSGLRPEDRYAHSLNFSLFIGGPCVLGAQK
LGALSIHAGTVPSERLLAILRQFQATAIWTTPSYAWYLGETAQKEGIDPKKDLAVKRIFVAGEPGGSIPETRARIEALWG
AEVYDYYGLSDIFGSCAGMCEEKDGLHWAEDHILVEVLDPETGEAVAPGERGEMVLTTLKKRARPLIRFRTGDIVSFTDE
PCACGRTSQRLLGVHGRRDDMLIVRGVNIFPSDVEAIVRKDHDFSGEYRLVIERIDHLDRLTIEVERAAGFQGTADALAD
RLAHRVKAITGVGAHIAVLDPDTLPRATHKAKRVEDRRSQVWTA

Specific function: Catalyzes the activation of phenylacetic acid to phenylacetyl-CoA [H]

COG id: COG1541

COG function: function code H; Coenzyme F390 synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787664, Length=445, Percent_Identity=37.5280898876404, Blast_Score=261, Evalue=8e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011880 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.30 [H]

Molecular weight: Translated: 49683; Mature: 49551

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRDTNQKYWDPQLETQSRAEWDALKLGLLKDHLKHAYDNSPYYRSSFDAAGVKPEDLKT
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
LADLQRFPTIDKKILRERQEAKPLLGDLAAVPERDVVYVSASSGSTGVPTVSPFTQQDFD
HHHHHHCCCHHHHHHHHHHHHCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHH
DWIDYEARQFWSSGLRPEDRYAHSLNFSLFIGGPCVLGAQKLGALSIHAGTVPSERLLAI
HHHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCEECHHHCCEEEEECCCCCHHHHHHH
LRQFQATAIWTTPSYAWYLGETAQKEGIDPKKDLAVKRIFVAGEPGGSIPETRARIEALW
HHHHHHEEEEECCCEEEEECCHHHHCCCCCHHHHEEEEEEEECCCCCCCCHHHHHHHHHH
GAEVYDYYGLSDIFGSCAGMCEEKDGLHWAEDHILVEVLDPETGEAVAPGERGEMVLTTL
CCHHHHHCCHHHHHHHHHCCCCCCCCCEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHH
KKRARPLIRFRTGDIVSFTDEPCACGRTSQRLLGVHGRRDDMLIVRGVNIFPSDVEAIVR
HHHCCCEEEEECCCEEEECCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHH
KDHDFSGEYRLVIERIDHLDRLTIEVERAAGFQGTADALADRLAHRVKAITGVGAHIAVL
CCCCCCCHHHHHHHHHHHHHHEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEE
DPDTLPRATHKAKRVEDRRSQVWTA
CCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PRDTNQKYWDPQLETQSRAEWDALKLGLLKDHLKHAYDNSPYYRSSFDAAGVKPEDLKT
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
LADLQRFPTIDKKILRERQEAKPLLGDLAAVPERDVVYVSASSGSTGVPTVSPFTQQDFD
HHHHHHCCCHHHHHHHHHHHHCHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHH
DWIDYEARQFWSSGLRPEDRYAHSLNFSLFIGGPCVLGAQKLGALSIHAGTVPSERLLAI
HHHHHHHHHHHHCCCCCCHHHHHCCCEEEEECCCCEECHHHCCEEEEECCCCCHHHHHHH
LRQFQATAIWTTPSYAWYLGETAQKEGIDPKKDLAVKRIFVAGEPGGSIPETRARIEALW
HHHHHHEEEEECCCEEEEECCHHHHCCCCCHHHHEEEEEEEECCCCCCCCHHHHHHHHHH
GAEVYDYYGLSDIFGSCAGMCEEKDGLHWAEDHILVEVLDPETGEAVAPGERGEMVLTTL
CCHHHHHCCHHHHHHHHHCCCCCCCCCEEECCCEEEEEECCCCCCCCCCCCCCCHHHHHH
KKRARPLIRFRTGDIVSFTDEPCACGRTSQRLLGVHGRRDDMLIVRGVNIFPSDVEAIVR
HHHCCCEEEEECCCEEEECCCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHH
KDHDFSGEYRLVIERIDHLDRLTIEVERAAGFQGTADALADRLAHRVKAITGVGAHIAVL
CCCCCCCHHHHHHHHHHHHHHEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEE
DPDTLPRATHKAKRVEDRRSQVWTA
CCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]