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The map label for this gene is opcM1 [H]

Identifier: 119898627

GI number: 119898627

Start: 2572063

End: 2573526

Strand: Direct

Name: opcM1 [H]

Synonym: azo2336

Alternate gene names: 119898627

Gene position: 2572063-2573526 (Clockwise)

Preceding gene: 119898626

Following gene: 119898628

Centisome position: 58.78

GC content: 74.86

Gene sequence:

>1464_bases
ATGCCGCGCCCCAAGGTCTTCGTCCTCGCCGCCGCGCTGCTGGTCGGCGGCTGCGCGCTCGGGCCGGACTTCCGCGCGCC
CGATGTCGCCCTTCCCGCCACCTTCCGCGCCGCCGAAGGCTGGCAGGTGGCCGTGCCGCAGGACATGCCCGCCACCGGCG
ACTGGTGGACGCGCTACCGCGACCCGCTGCTCGACCAGTTGCTGGCGCGCCTGAACGCCGACAACCTTGAAGTGGCCGCC
GCCGCGGCGCGCTATCGCGAAGCACTCGCCTTCGCCCGCGGCACCCGCGCCAACGCGCTGCCCACGCTCGACGCGGGGCT
GGCCGGCAGCCGCAGCCGCACCGCCGCGAGCGACAGCAGCAGCGATCCAGCCGCGCGCAACCTGCAACGCTTCAGCCTCA
CCAGCCGCTGGGAGATCGACCTGTGGGGCCGGCTCGCGCGCAACACCGAAGCCGACCGCCACGATGCCGCCGCCAGCGGC
GCCGACCTCGCCGCCCTGCGGCTGGCGCTGCAGGCCACGCTGGCGCAGGACTACCTGCAACTGCGCGTCATCGACGCCCA
ACAGCGGCTCTACCAACGCACTGTCGCCACCTACCGGCGTTCGCTGGAGATCACGACCAACCGCCACCGCGCGGGCGTCG
CCAGCCTGGCCGAGGTGGCGCAAGCCGAAACCCAGCTGCGCAGCGCCGAGGCCCAGGGCATCGACCTGCAGCTGCAACGC
GCCCGGCTGGAACACGCGATCGCCGTGCTGGTGGGCAGCGCGCCTTCGCAGCTGCGCATCGAGCCGGTTGCCGCGGTGCC
CGAACTGCCGCCGCTGCCGGCCGCGCAACCGGCCGAGGTGCTGCAGCGCCGTCCCGACGTGGCTGCCGCGCTGCGTCGCG
TGGCCGCCGGCAATGCCCGCATCGGCGCGGCGGAGGCCGCCTTCTTCCCCACGCTGGTGCTGGACGCTTCCGCCGGCTAT
CAGCACGAGCGCCTCGCCGACCTCTTCGCGCTGCCGCACCGCTTCTGGTCGCTGGGGCCGACACTGGCCCAGCACCTGTT
CGACGGCGGGGCCCGCCGCGCCGCGCGCGACCAGGCCGAAGCCGCCTACGACCGCAGCGTGGCCGACTACCGCCAGACCG
TGCTGGACGCCTTCCGCGAAGCGGAGGACAGCCTGGCCGGGTTGCGCATCCTGGGCGACGAGCGCCAGGTTCAGGCCGCC
GCCACCCGCGCCGCCAACGAGTTCCTGGCGCAGACCAACAACCAGTACCTCGCCGGCACCGTCAGCTACCTCAACGTCGC
CATCGCCCAGGCCGCGGCGCTGACCGCCGAGCGCGCCAGCCTCGACCTGCTCGGGCGCCAGCTCGCGGCCTCGGTCGAGC
TGGTGCGCGCGCTCGGCGGCGACCCGTGGGAAGCCGCGCTGGCCAAGGAACACCCCGCCCCCGCCGTCGCGCCCGCGCCG
GTGCCGTCCCCACCTTTGTCCTGA

Upstream 100 bases:

>100_bases
ATCCCGCCTGACGCCCCCAATGAAACCGCACACCGCGTCACCCGTCGCCGCCTTGCCCGCCATGACCCCACCACCACCTC
ACCACGCCGCCCGCCAACGC

Downstream 100 bases:

>100_bases
CCGGAGATCCGTCCATGAAGCGCGTGCATATCTGGGACCTGCCCACCCGCCTCTTCCACTGGCTGCTGGCCTTCGCCATC
CTCGCCGCCTACGTCACCGG

Product: putative outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MPRPKVFVLAAALLVGGCALGPDFRAPDVALPATFRAAEGWQVAVPQDMPATGDWWTRYRDPLLDQLLARLNADNLEVAA
AAARYREALAFARGTRANALPTLDAGLAGSRSRTAASDSSSDPAARNLQRFSLTSRWEIDLWGRLARNTEADRHDAAASG
ADLAALRLALQATLAQDYLQLRVIDAQQRLYQRTVATYRRSLEITTNRHRAGVASLAEVAQAETQLRSAEAQGIDLQLQR
ARLEHAIAVLVGSAPSQLRIEPVAAVPELPPLPAAQPAEVLQRRPDVAAALRRVAAGNARIGAAEAAFFPTLVLDASAGY
QHERLADLFALPHRFWSLGPTLAQHLFDGGARRAARDQAEAAYDRSVADYRQTVLDAFREAEDSLAGLRILGDERQVQAA
ATRAANEFLAQTNNQYLAGTVSYLNVAIAQAAALTAERASLDLLGRQLAASVELVRALGGDPWEAALAKEHPAPAVAPAP
VPSPPLS

Sequences:

>Translated_487_residues
MPRPKVFVLAAALLVGGCALGPDFRAPDVALPATFRAAEGWQVAVPQDMPATGDWWTRYRDPLLDQLLARLNADNLEVAA
AAARYREALAFARGTRANALPTLDAGLAGSRSRTAASDSSSDPAARNLQRFSLTSRWEIDLWGRLARNTEADRHDAAASG
ADLAALRLALQATLAQDYLQLRVIDAQQRLYQRTVATYRRSLEITTNRHRAGVASLAEVAQAETQLRSAEAQGIDLQLQR
ARLEHAIAVLVGSAPSQLRIEPVAAVPELPPLPAAQPAEVLQRRPDVAAALRRVAAGNARIGAAEAAFFPTLVLDASAGY
QHERLADLFALPHRFWSLGPTLAQHLFDGGARRAARDQAEAAYDRSVADYRQTVLDAFREAEDSLAGLRILGDERQVQAA
ATRAANEFLAQTNNQYLAGTVSYLNVAIAQAAALTAERASLDLLGRQLAASVELVRALGGDPWEAALAKEHPAPAVAPAP
VPSPPLS
>Mature_486_residues
PRPKVFVLAAALLVGGCALGPDFRAPDVALPATFRAAEGWQVAVPQDMPATGDWWTRYRDPLLDQLLARLNADNLEVAAA
AARYREALAFARGTRANALPTLDAGLAGSRSRTAASDSSSDPAARNLQRFSLTSRWEIDLWGRLARNTEADRHDAAASGA
DLAALRLALQATLAQDYLQLRVIDAQQRLYQRTVATYRRSLEITTNRHRAGVASLAEVAQAETQLRSAEAQGIDLQLQRA
RLEHAIAVLVGSAPSQLRIEPVAAVPELPPLPAAQPAEVLQRRPDVAAALRRVAAGNARIGAAEAAFFPTLVLDASAGYQ
HERLADLFALPHRFWSLGPTLAQHLFDGGARRAARDQAEAAYDRSVADYRQTVLDAFREAEDSLAGLRILGDERQVQAAA
TRAANEFLAQTNNQYLAGTVSYLNVAIAQAAALTAERASLDLLGRQLAASVELVRALGGDPWEAALAKEHPAPAVAPAPV
PSPPLS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=463, Percent_Identity=26.9978401727862, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI2367348, Length=475, Percent_Identity=26.7368421052632, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52131; Mature: 52000

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRPKVFVLAAALLVGGCALGPDFRAPDVALPATFRAAEGWQVAVPQDMPATGDWWTRYR
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCEEECCCCCCCCCCHHHHHH
DPLLDQLLARLNADNLEVAAAAARYREALAFARGTRANALPTLDAGLAGSRSRTAASDSS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SDPAARNLQRFSLTSRWEIDLWGRLARNTEADRHDAAASGADLAALRLALQATLAQDYLQ
CCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LRVIDAQQRLYQRTVATYRRSLEITTNRHRAGVASLAEVAQAETQLRSAEAQGIDLQLQR
HHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ARLEHAIAVLVGSAPSQLRIEPVAAVPELPPLPAAQPAEVLQRRPDVAAALRRVAAGNAR
HHHHHHHHHHCCCCCCCEEEECHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCC
IGAAEAAFFPTLVLDASAGYQHERLADLFALPHRFWSLGPTLAQHLFDGGARRAARDQAE
CCCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHH
AAYDRSVADYRQTVLDAFREAEDSLAGLRILGDERQVQAAATRAANEFLAQTNNQYLAGT
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEHH
VSYLNVAIAQAAALTAERASLDLLGRQLAASVELVRALGGDPWEAALAKEHPAPAVAPAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCC
VPSPPLS
CCCCCCC
>Mature Secondary Structure 
PRPKVFVLAAALLVGGCALGPDFRAPDVALPATFRAAEGWQVAVPQDMPATGDWWTRYR
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCEEECCCCCCCCCCHHHHHH
DPLLDQLLARLNADNLEVAAAAARYREALAFARGTRANALPTLDAGLAGSRSRTAASDSS
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SDPAARNLQRFSLTSRWEIDLWGRLARNTEADRHDAAASGADLAALRLALQATLAQDYLQ
CCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LRVIDAQQRLYQRTVATYRRSLEITTNRHRAGVASLAEVAQAETQLRSAEAQGIDLQLQR
HHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
ARLEHAIAVLVGSAPSQLRIEPVAAVPELPPLPAAQPAEVLQRRPDVAAALRRVAAGNAR
HHHHHHHHHHCCCCCCCEEEECHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHCCCCC
IGAAEAAFFPTLVLDASAGYQHERLADLFALPHRFWSLGPTLAQHLFDGGARRAARDQAE
CCCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHH
AAYDRSVADYRQTVLDAFREAEDSLAGLRILGDERQVQAAATRAANEFLAQTNNQYLAGT
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEHH
VSYLNVAIAQAAALTAERASLDLLGRQLAASVELVRALGGDPWEAALAKEHPAPAVAPAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCC
VPSPPLS
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]