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The map label for this gene is pspF [H]

Identifier: 119898619

GI number: 119898619

Start: 2560607

End: 2561695

Strand: Direct

Name: pspF [H]

Synonym: azo2328

Alternate gene names: 119898619

Gene position: 2560607-2561695 (Clockwise)

Preceding gene: 119898617

Following gene: 119898620

Centisome position: 58.51

GC content: 65.66

Gene sequence:

>1089_bases
ATGAGCGAATCGAGTCCGGGAAAACTGCTGACCCTTCCCAATGCCGAGAACCAGGCACTGACAATCCGCGCCAAGGCACT
GATTTTCCACGACAGAAAGTCGCTGGCCCTGTTGCGGCATGTTGAACGCGTCGCCCCCAGCGATGCAAATGTATTGATCA
TCGGTGAAACCGGCACCGGCAAGGAATTGATTGCGCGCCATATCCACGCATTGAGCGGCCGCCGTGGGCCCTTCATTGCA
GTCAATTGCGGTGCCTTTTCCGAGAACCTGGTCGAGGCCGAATTGTTCGGCCATGAAGCCGGCGCATTCACCGGCGCCCA
GCAGGCGCGCGCCGGCTGGTTCGAAGCGGCCAATGGCGGCACGCTGTTTCTCGACGAAGTGGGCGATCTGCCGCTCGCGA
TCCAGGTGAAATTGCTGCGCGTGCTGCAGGAACGCCAGGTGGTGCGGCTCGGTTCGCGCAAATCGACGCCGGTGGATGTG
CGGCTGGTCGCGGCCACCAACGTCGATCTGGAAAAGGCCGTGGCAGCCGGGCATTTCCGCCTCGATCTCTATTACCGCCT
GAACGTCGCCCCGGTGATGCTGCCCGCATTGCGCGAGCGCGCCACCGACATCCTGCCGCTGGTCGATTATTTCATCGAGT
ACTACCGCCACCGGCTCGGCATCGACGCGCCCACGCTGAGCCCGGAAGCCGAAACCCTGCTCACCCGCTACCACTGGCCG
GGCAACATCCGCGAGCTGGAGAACGTGGTGCATTTCGCCCTCATCGTCTGCCAGGACGGCATCGTCACGCCCGCCGACCT
GCGCCTGCCGGGCGTAACCGGACCCGGCGCCGCGGGTGCGGCCCCGACCGGCGGCGATCCGTTCGCCTCGCTGCAGGTGC
TGGTGTGCCGGCTGCTGGAAGGCGGCACGCCGGCGCTGTACGAGCAGATCGAGCGCCTGCTGTTCACCACCGCGTTCGAG
TACTGCCGCGAGAACCAGGTGCGCACCGCGCGCGAGCTGGGCATCTCGCGCAACATCCTGCGCACCCAGCTCAAGCGCTT
CGGCCTGATCGGCGACGCCACGCCCGAGACCGCGGACGCCTTCGACTAG

Upstream 100 bases:

>100_bases
TTGATGACAGCCGCTCCCTCCCCGGGATCGCCCCATAACAAGCATTCGACCGGCCGCCTTCGCGAGCCGGTTGGTTTCAT
CCCTGCTGGTAGAGAAACCC

Downstream 100 bases:

>100_bases
GCCTCGGCTGCTGCGGCGCAGCACCTGCTGCGCGCGCAGCAGCCGCTTTCACCGGTTGCCGCGCAGCCAGCACCGCGCGC
CGCGCGTTTCGCGCCCAGCG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSESSPGKLLTLPNAENQALTIRAKALIFHDRKSLALLRHVERVAPSDANVLIIGETGTGKELIARHIHALSGRRGPFIA
VNCGAFSENLVEAELFGHEAGAFTGAQQARAGWFEAANGGTLFLDEVGDLPLAIQVKLLRVLQERQVVRLGSRKSTPVDV
RLVAATNVDLEKAVAAGHFRLDLYYRLNVAPVMLPALRERATDILPLVDYFIEYYRHRLGIDAPTLSPEAETLLTRYHWP
GNIRELENVVHFALIVCQDGIVTPADLRLPGVTGPGAAGAAPTGGDPFASLQVLVCRLLEGGTPALYEQIERLLFTTAFE
YCRENQVRTARELGISRNILRTQLKRFGLIGDATPETADAFD

Sequences:

>Translated_362_residues
MSESSPGKLLTLPNAENQALTIRAKALIFHDRKSLALLRHVERVAPSDANVLIIGETGTGKELIARHIHALSGRRGPFIA
VNCGAFSENLVEAELFGHEAGAFTGAQQARAGWFEAANGGTLFLDEVGDLPLAIQVKLLRVLQERQVVRLGSRKSTPVDV
RLVAATNVDLEKAVAAGHFRLDLYYRLNVAPVMLPALRERATDILPLVDYFIEYYRHRLGIDAPTLSPEAETLLTRYHWP
GNIRELENVVHFALIVCQDGIVTPADLRLPGVTGPGAAGAAPTGGDPFASLQVLVCRLLEGGTPALYEQIERLLFTTAFE
YCRENQVRTARELGISRNILRTQLKRFGLIGDATPETADAFD
>Mature_361_residues
SESSPGKLLTLPNAENQALTIRAKALIFHDRKSLALLRHVERVAPSDANVLIIGETGTGKELIARHIHALSGRRGPFIAV
NCGAFSENLVEAELFGHEAGAFTGAQQARAGWFEAANGGTLFLDEVGDLPLAIQVKLLRVLQERQVVRLGSRKSTPVDVR
LVAATNVDLEKAVAAGHFRLDLYYRLNVAPVMLPALRERATDILPLVDYFIEYYRHRLGIDAPTLSPEAETLLTRYHWPG
NIRELENVVHFALIVCQDGIVTPADLRLPGVTGPGAAGAAPTGGDPFASLQVLVCRLLEGGTPALYEQIERLLFTTAFEY
CRENQVRTARELGISRNILRTQLKRFGLIGDATPETADAFD

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG1221

COG function: function code KT; Transcriptional regulators containing an AAA-type ATPase domain and a DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=320, Percent_Identity=44.375, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1789233, Length=317, Percent_Identity=41.9558359621451, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1790437, Length=334, Percent_Identity=42.5149700598802, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1790299, Length=319, Percent_Identity=43.5736677115987, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1789087, Length=317, Percent_Identity=44.794952681388, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI87082117, Length=233, Percent_Identity=51.0729613733906, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI87082152, Length=252, Percent_Identity=49.2063492063492, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1788905, Length=232, Percent_Identity=49.5689655172414, Blast_Score=217, Evalue=8e-58,
Organism=Escherichia coli, GI87081872, Length=321, Percent_Identity=42.3676012461059, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1786524, Length=223, Percent_Identity=50.6726457399103, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI1787583, Length=317, Percent_Identity=35.9621451104101, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1789828, Length=238, Percent_Identity=37.8151260504202, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI87081858, Length=242, Percent_Identity=33.0578512396694, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 39543; Mature: 39412

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESSPGKLLTLPNAENQALTIRAKALIFHDRKSLALLRHVERVAPSDANVLIIGETGTG
CCCCCCCCEEECCCCCCCEEEEEEEEEEEECHHHHHHHHHHHHHCCCCCCEEEEECCCCC
KELIARHIHALSGRRGPFIAVNCGAFSENLVEAELFGHEAGAFTGAQQARAGWFEAANGG
HHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHCCCEECCCCC
TLFLDEVGDLPLAIQVKLLRVLQERQVVRLGSRKSTPVDVRLVAATNVDLEKAVAAGHFR
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHCCEEE
LDLYYRLNVAPVMLPALRERATDILPLVDYFIEYYRHRLGIDAPTLSPEAETLLTRYHWP
EEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCC
GNIRELENVVHFALIVCQDGIVTPADLRLPGVTGPGAAGAAPTGGDPFASLQVLVCRLLE
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
GGTPALYEQIERLLFTTAFEYCRENQVRTARELGISRNILRTQLKRFGLIGDATPETADA
CCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
FD
CC
>Mature Secondary Structure 
SESSPGKLLTLPNAENQALTIRAKALIFHDRKSLALLRHVERVAPSDANVLIIGETGTG
CCCCCCCEEECCCCCCCEEEEEEEEEEEECHHHHHHHHHHHHHCCCCCCEEEEECCCCC
KELIARHIHALSGRRGPFIAVNCGAFSENLVEAELFGHEAGAFTGAQQARAGWFEAANGG
HHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCHHHHHCCCEECCCCC
TLFLDEVGDLPLAIQVKLLRVLQERQVVRLGSRKSTPVDVRLVAATNVDLEKAVAAGHFR
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHCCEEE
LDLYYRLNVAPVMLPALRERATDILPLVDYFIEYYRHRLGIDAPTLSPEAETLLTRYHWP
EEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCC
GNIRELENVVHFALIVCQDGIVTPADLRLPGVTGPGAAGAAPTGGDPFASLQVLVCRLLE
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
GGTPALYEQIERLLFTTAFEYCRENQVRTARELGISRNILRTQLKRFGLIGDATPETADA
CCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
FD
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11243806 [H]