Click here to switch to the map view.

The map label for this gene is pgdS [H]

Identifier: 119898494

GI number: 119898494

Start: 2413954

End: 2414493

Strand: Direct

Name: pgdS [H]

Synonym: azo2203

Alternate gene names: 119898494

Gene position: 2413954-2414493 (Clockwise)

Preceding gene: 119898493

Following gene: 119898496

Centisome position: 55.16

GC content: 67.59

Gene sequence:

>540_bases
ATGCAGATTCGTGCCCCCCTCCTCGCTCTCGCCCTGAATCTCGCCTGGATTCCCGCCGCGCTGCCGGCGGAGGCCGCGAC
CTCCCAGACTTCCGCGACCGCGACCGCCGGTGACGACTACGCCACCGCCGCCCAGGAGTTGATCAACCAGGGCATGAGCT
ACCTCGGAATCCGCTACCGCTTCGGCGGCAGCAGCCCCGAATCCGGCCTGGACTGCAGCGGGCTGGTGCAGAACGTGTTC
CGTAATGCGCTGGGGCTGGACCTGCCGCGCACCGCGCGCGAGATGTCCAATCTCGGGGACAAGATCGGTCGTCAGGAACT
CAAGCCGGGCGATCTGGTGTTCTTCAACACCATGCGGCGCGCTTTCTCTCACGTCGGCATCTACGTGGGAGACGGTCAGT
TCCTGCATGCGCCGTCGCGTGGCGGCGGCGTGCGCGTGGAAGACATGGGCACCAGCTACTGGGCAAAGCGCTTCAACGGC
GCCCGCCGCCTGCTGGACGACGAGAAGGCCACCGCCGAACTCCCGCCCTTCCCGCGCTGA

Upstream 100 bases:

>100_bases
CTGTGGCGCCAGCCCTGACCCCGCAAAGCTGAAAATTAATGCGTTTTTTCATTGCAATCCGAGGTTTACACTGCGCCATC
GGGTGCGTATAGTCGCGCCG

Downstream 100 bases:

>100_bases
CGCGGGCGCCGGCAGCCCGGAACTGCGGCACCGGCAGATCTTCCTTCCTTACTTACTTGCTTCCTCGGCGGCCGGGCCGG
CAGGCTGCGGCGCACCCGTT

Product: putative lipoprotein precusor

Products: NA

Alternate protein names: Poly-gamma-glutamate depolymerase; PGA depolymerase [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MQIRAPLLALALNLAWIPAALPAEAATSQTSATATAGDDYATAAQELINQGMSYLGIRYRFGGSSPESGLDCSGLVQNVF
RNALGLDLPRTAREMSNLGDKIGRQELKPGDLVFFNTMRRAFSHVGIYVGDGQFLHAPSRGGGVRVEDMGTSYWAKRFNG
ARRLLDDEKATAELPPFPR

Sequences:

>Translated_179_residues
MQIRAPLLALALNLAWIPAALPAEAATSQTSATATAGDDYATAAQELINQGMSYLGIRYRFGGSSPESGLDCSGLVQNVF
RNALGLDLPRTAREMSNLGDKIGRQELKPGDLVFFNTMRRAFSHVGIYVGDGQFLHAPSRGGGVRVEDMGTSYWAKRFNG
ARRLLDDEKATAELPPFPR
>Mature_179_residues
MQIRAPLLALALNLAWIPAALPAEAATSQTSATATAGDDYATAAQELINQGMSYLGIRYRFGGSSPESGLDCSGLVQNVF
RNALGLDLPRTAREMSNLGDKIGRQELKPGDLVFFNTMRRAFSHVGIYVGDGQFLHAPSRGGGVRVEDMGTSYWAKRFNG
ARRLLDDEKATAELPPFPR

Specific function: Cleaves, in an endo-type manner, the gamma-glutamyl bond between D-glutamate and L-glutamate of poly-gamma-glutamate (PGA) [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Secreted. Secreted, cell wall. Note=Cell wall localization shown in PubMed:11987133 [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nlpC/p60 family [H]

Homologues:

Organism=Escherichia coli, GI1787944, Length=144, Percent_Identity=40.2777777777778, Blast_Score=118, Evalue=2e-28,
Organism=Escherichia coli, GI1788501, Length=176, Percent_Identity=38.6363636363636, Blast_Score=116, Evalue=7e-28,
Organism=Escherichia coli, GI1786421, Length=159, Percent_Identity=35.2201257861635, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1788001, Length=127, Percent_Identity=40.1574803149606, Blast_Score=88, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064 [H]

Pfam domain/function: PF00877 NLPC_P60 [H]

EC number: NA

Molecular weight: Translated: 19346; Mature: 19346

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIRAPLLALALNLAWIPAALPAEAATSQTSATATAGDDYATAAQELINQGMSYLGIRYR
CCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHEEEEE
FGGSSPESGLDCSGLVQNVFRNALGLDLPRTAREMSNLGDKIGRQELKPGDLVFFNTMRR
ECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHH
AFSHVGIYVGDGQFLHAPSRGGGVRVEDMGTSYWAKRFNGARRLLDDEKATAELPPFPR
HHHHCCEEECCCCEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQIRAPLLALALNLAWIPAALPAEAATSQTSATATAGDDYATAAQELINQGMSYLGIRYR
CCCCHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHEEEEE
FGGSSPESGLDCSGLVQNVFRNALGLDLPRTAREMSNLGDKIGRQELKPGDLVFFNTMRR
ECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHH
AFSHVGIYVGDGQFLHAPSRGGGVRVEDMGTSYWAKRFNGARRLLDDEKATAELPPFPR
HHHHCCEEECCCCEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12073126; 12644511; 9353933; 9384377; 10658653 [H]