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The map label for this gene is mdeA [H]

Identifier: 119898484

GI number: 119898484

Start: 2398921

End: 2400192

Strand: Direct

Name: mdeA [H]

Synonym: azo2193

Alternate gene names: 119898484

Gene position: 2398921-2400192 (Clockwise)

Preceding gene: 119898482

Following gene: 119898486

Centisome position: 54.82

GC content: 65.41

Gene sequence:

>1272_bases
ATGAAACTGGAGACGATTGCGGTACACGGCGGTTATTCCCCGGATCCCACCACGAAAGCGGTAGCGGTCCCGATTTACCA
GACCACCTCGTACGCCTTCGATGACACCCAGCATGGCGCCGACCTGTTCGACCTGAAGGTCCAGGGCAACATCTACACCC
GCATCATGAACCCGACGACGGCGGTGCTGGAGCAGCGCGTTGCGGCGCTGGAAGGCGGCATCGGCGCGCTCGCGGTCGCC
TCAGGCATGGCTGCGATCACCTACGCCATTCAGACGATTGCCGAAGCGGGCGACAACATTGTCTCGGCATCCACGCTTTA
CGGCGGCACCTACAACCTGTTTGCCCACACGCTGCCGCAACTCGGCGTGGAAGTGCGCTTTGCCGACCAGCGCGATCCGG
CAAGTTTCGGCCGCTTGATCGACGAGCGCACCAAGGCCATCTTCTGCGAGTCGGTCGGCAACCCGCTCGGCAACATCACG
GACTTCGCCGCGCTGGCCGCGGTAGCCCACGCGAACGGCATTCCGTTGATCGTCGACAACACCGTCCCCACGCCCTACCT
CTGCCGGCCGTTCGAGCACGGCGCGGACATCGTGGTGCATTCGCTGACGAAGTATCTGGGCGGCCACGGCAACTCGATCG
GCGGCATCATCGTGGACTCGGGCAAATTCCCCTGGCGCCAGCACAAGGAGCGCTTCCGCAGGCTCAACGAGCCTGACGTC
TCGTACCACGGCGTGGTCTACACCGAAGCGCTGGGCGAAGCGGCCTACATCGGGCGCGCACGGGTGGTTCCGCTGCGCAA
CACCGGGGCCGCGCTCAGCCCCTTCAATGCCTTCCTGATCCTGCAGGGCATCGAAACGCTGGCCCTGCGCATGGACCGCA
TCTGCGCGAATACCCTGGAGGTGGCGCGTTTCCTCAAGCAGCACGGGAAGGTGGAGTGGGTGAATTACGCCGGTCTGCCC
GACCACGCCGACCATGGCCTGGTGCAGAAGTATCTGGGCGGCCACGCCTCCGGCATCCTCTCCTTCGGCGTAAAGGGCGG
CTACGCCGCGGGCGGACGCTTCCAGGACGCGCTGAAGCTCGTCACCCGCCTGGTGAACATCGGCGATGCAAAATCGCTCG
CGTGCCATCCGGCCTCGACCACGCACCGCCAGCTGGCACCGGCGGAACTGGCCAAGGCCGGCGTGTCGCCTGACATGGTC
CGCCTGTCGATCGGCATCGAGCACATCGACGATCTGCTTGCCGACATCGGTCAGGCGCTCGACGCGGCCTGA

Upstream 100 bases:

>100_bases
GAAAGCCCCACCGAAATTCAAATCGAAACTACTTATTTCGCCCGGAATGAAACAGGTGGCTATACTCGCCCCCCTTTTCC
ATGACAAGGGAGGATGTGGA

Downstream 100 bases:

>100_bases
TGAGGTAAGGCGGGCAGCCCCGGCTGCCCGCCGCCTTTAACGGAGCGCGAACTCCACGCGACCCGCCGTTTCCGCACCCG
CACCGCCGACACGGGGCGCA

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MKLETIAVHGGYSPDPTTKAVAVPIYQTTSYAFDDTQHGADLFDLKVQGNIYTRIMNPTTAVLEQRVAALEGGIGALAVA
SGMAAITYAIQTIAEAGDNIVSASTLYGGTYNLFAHTLPQLGVEVRFADQRDPASFGRLIDERTKAIFCESVGNPLGNIT
DFAALAAVAHANGIPLIVDNTVPTPYLCRPFEHGADIVVHSLTKYLGGHGNSIGGIIVDSGKFPWRQHKERFRRLNEPDV
SYHGVVYTEALGEAAYIGRARVVPLRNTGAALSPFNAFLILQGIETLALRMDRICANTLEVARFLKQHGKVEWVNYAGLP
DHADHGLVQKYLGGHASGILSFGVKGGYAAGGRFQDALKLVTRLVNIGDAKSLACHPASTTHRQLAPAELAKAGVSPDMV
RLSIGIEHIDDLLADIGQALDAA

Sequences:

>Translated_423_residues
MKLETIAVHGGYSPDPTTKAVAVPIYQTTSYAFDDTQHGADLFDLKVQGNIYTRIMNPTTAVLEQRVAALEGGIGALAVA
SGMAAITYAIQTIAEAGDNIVSASTLYGGTYNLFAHTLPQLGVEVRFADQRDPASFGRLIDERTKAIFCESVGNPLGNIT
DFAALAAVAHANGIPLIVDNTVPTPYLCRPFEHGADIVVHSLTKYLGGHGNSIGGIIVDSGKFPWRQHKERFRRLNEPDV
SYHGVVYTEALGEAAYIGRARVVPLRNTGAALSPFNAFLILQGIETLALRMDRICANTLEVARFLKQHGKVEWVNYAGLP
DHADHGLVQKYLGGHASGILSFGVKGGYAAGGRFQDALKLVTRLVNIGDAKSLACHPASTTHRQLAPAELAKAGVSPDMV
RLSIGIEHIDDLLADIGQALDAA
>Mature_423_residues
MKLETIAVHGGYSPDPTTKAVAVPIYQTTSYAFDDTQHGADLFDLKVQGNIYTRIMNPTTAVLEQRVAALEGGIGALAVA
SGMAAITYAIQTIAEAGDNIVSASTLYGGTYNLFAHTLPQLGVEVRFADQRDPASFGRLIDERTKAIFCESVGNPLGNIT
DFAALAAVAHANGIPLIVDNTVPTPYLCRPFEHGADIVVHSLTKYLGGHGNSIGGIIVDSGKFPWRQHKERFRRLNEPDV
SYHGVVYTEALGEAAYIGRARVVPLRNTGAALSPFNAFLILQGIETLALRMDRICANTLEVARFLKQHGKVEWVNYAGLP
DHADHGLVQKYLGGHASGILSFGVKGGYAAGGRFQDALKLVTRLVNIGDAKSLACHPASTTHRQLAPAELAKAGVSPDMV
RLSIGIEHIDDLLADIGQALDAA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=422, Percent_Identity=34.1232227488152, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI299473759, Length=422, Percent_Identity=30.5687203791469, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI25453487, Length=421, Percent_Identity=31.353919239905, Blast_Score=142, Evalue=8e-34,
Organism=Escherichia coli, GI1790375, Length=422, Percent_Identity=31.5165876777251, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1789383, Length=421, Percent_Identity=26.1282660332542, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71980708, Length=418, Percent_Identity=31.3397129186603, Blast_Score=207, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI17560056, Length=424, Percent_Identity=34.9056603773585, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=424, Percent_Identity=33.4905660377358, Blast_Score=189, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=48.3796296296296, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6319307, Length=431, Percent_Identity=32.9466357308585, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321254, Length=444, Percent_Identity=25.9009009009009, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=348, Percent_Identity=28.1609195402299, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321904, Length=430, Percent_Identity=24.8837209302326, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922554, Length=425, Percent_Identity=33.6470588235294, Blast_Score=198, Evalue=6e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45131; Mature: 45131

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLETIAVHGGYSPDPTTKAVAVPIYQTTSYAFDDTQHGADLFDLKVQGNIYTRIMNPTT
CCEEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCHHH
AVLEQRVAALEGGIGALAVASGMAAITYAIQTIAEAGDNIVSASTLYGGTYNLFAHTLPQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHEECCCHHHHHHHHHHH
LGVEVRFADQRDPASFGRLIDERTKAIFCESVGNPLGNITDFAALAAVAHANGIPLIVDN
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECC
TVPTPYLCRPFEHGADIVVHSLTKYLGGHGNSIGGIIVDSGKFPWRQHKERFRRLNEPDV
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCC
SYHGVVYTEALGEAAYIGRARVVPLRNTGAALSPFNAFLILQGIETLALRMDRICANTLE
CCCCEEEHHHHCCHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VARFLKQHGKVEWVNYAGLPDHADHGLVQKYLGGHASGILSFGVKGGYAAGGRFQDALKL
HHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHH
VTRLVNIGDAKSLACHPASTTHRQLAPAELAKAGVSPDMVRLSIGIEHIDDLLADIGQAL
HHHHHCCCCCCCEEECCCCCCHHHCCHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHH
DAA
CCC
>Mature Secondary Structure
MKLETIAVHGGYSPDPTTKAVAVPIYQTTSYAFDDTQHGADLFDLKVQGNIYTRIMNPTT
CCEEEEEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCHHH
AVLEQRVAALEGGIGALAVASGMAAITYAIQTIAEAGDNIVSASTLYGGTYNLFAHTLPQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHEECCCHHHHHHHHHHH
LGVEVRFADQRDPASFGRLIDERTKAIFCESVGNPLGNITDFAALAAVAHANGIPLIVDN
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEECC
TVPTPYLCRPFEHGADIVVHSLTKYLGGHGNSIGGIIVDSGKFPWRQHKERFRRLNEPDV
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCC
SYHGVVYTEALGEAAYIGRARVVPLRNTGAALSPFNAFLILQGIETLALRMDRICANTLE
CCCCEEEHHHHCCHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VARFLKQHGKVEWVNYAGLPDHADHGLVQKYLGGHASGILSFGVKGGYAAGGRFQDALKL
HHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHH
VTRLVNIGDAKSLACHPASTTHRQLAPAELAKAGVSPDMVRLSIGIEHIDDLLADIGQAL
HHHHHCCCCCCCEEECCCCCCHHHCCHHHHHHCCCCCCEEEEEECHHHHHHHHHHHHHHH
DAA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]