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The map label for this gene is truB

Identifier: 119898396

GI number: 119898396

Start: 2311618

End: 2312520

Strand: Reverse

Name: truB

Synonym: azo2105

Alternate gene names: 119898396

Gene position: 2312520-2311618 (Counterclockwise)

Preceding gene: 119898397

Following gene: 119898395

Centisome position: 52.85

GC content: 65.89

Gene sequence:

>903_bases
ATGCAGCGCAAGATTCCGCGACGCATCGTGGACGGCGTCCTATTGCTGGACAAGCCGAGCGGCATGACCAGCAATGGCGC
GCTGCAGACGGCCCGGCGCTTGTTGAATGCTGCCAAGGCCGGTCACACCGGCACGCTCGACCCGATGGCAAGCGGGCTGT
TGCCGCTGACGTTCGGGGAAGCGACGAAGTTTTCCCAGATCCTGCTCGACGCGGACAAGACCTACGAGGCGGGCGTGAAG
CTCGGCACCACCACCGACACCGGCGACGCCGATGGCAATGTGGTTGCGGAGCATCCGGTTTCCGTCACGCGCGAGGCGCT
GGAAGAGGTGCTCTCGCGCTTCCGCGGCGAAATCGACCAGCTTCCGCCGATGTATTCGGCGCTGAAGCGCGACGGCAAGC
CGCTCTACGAGTATGCCCGCGCCGGGATCGAGATCGAGCGGGAAGTCCGGCGGGTCACAATCCACGACCTCGAACTGATT
GCGTTCTCCGGTGAGCACTTCAGCATGCGGGTCCGTTGCAGCAAGGGTACCTACATCCGCACGCTGGCGATGGACATCGG
CGCGGCGCTGGGTTGCGGCGCTTACCTCGATGCCCTGCGGCGCACTGCCATCGGCGATTTCGACGCGGCTCGTGCGGTGA
CGCTGGAAGCCCTGGAAGCGAGTCCGGCGGCGATGCGGGACGGTCTGCTCGAGCCGGTGGATGCGCTCGTGGCGCACTTT
CCCAAAGTCGAACTGCAGCCCGCGGAAGCGGCCGCGATTCTCCAGGGACGCGAGTTGCGGAAACCGGAAGACGGGCAGGG
CAGCGTGCGCCTTTTCTGCGGCGGTCGCTTTCTGGGTGTCGGCGAGTGGCAATCCGGTTCTCTGCGGCCCAAACGGCTCA
TCGCCACGCAAACCGGGCAATGA

Upstream 100 bases:

>100_bases
GTCGAGGAGGGTAGCCGGTTGTCCCGCCTGATCGACGACGCCGTGCGCGAAGACGAAATCCGTCACCGCGACGACGACAA
CTAGCTTTCCGAGGCTTCCC

Downstream 100 bases:

>100_bases
TGCCGGCGCGCATATTTTCGATTGAATAAACCGGAGTGACGCGGTTATAATCCGCCGCTTCTGATTGGCAGAACGAATAG
AGTAAAGACATCAATGGCTC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MQRKIPRRIVDGVLLLDKPSGMTSNGALQTARRLLNAAKAGHTGTLDPMASGLLPLTFGEATKFSQILLDADKTYEAGVK
LGTTTDTGDADGNVVAEHPVSVTREALEEVLSRFRGEIDQLPPMYSALKRDGKPLYEYARAGIEIEREVRRVTIHDLELI
AFSGEHFSMRVRCSKGTYIRTLAMDIGAALGCGAYLDALRRTAIGDFDAARAVTLEALEASPAAMRDGLLEPVDALVAHF
PKVELQPAEAAAILQGRELRKPEDGQGSVRLFCGGRFLGVGEWQSGSLRPKRLIATQTGQ

Sequences:

>Translated_300_residues
MQRKIPRRIVDGVLLLDKPSGMTSNGALQTARRLLNAAKAGHTGTLDPMASGLLPLTFGEATKFSQILLDADKTYEAGVK
LGTTTDTGDADGNVVAEHPVSVTREALEEVLSRFRGEIDQLPPMYSALKRDGKPLYEYARAGIEIEREVRRVTIHDLELI
AFSGEHFSMRVRCSKGTYIRTLAMDIGAALGCGAYLDALRRTAIGDFDAARAVTLEALEASPAAMRDGLLEPVDALVAHF
PKVELQPAEAAAILQGRELRKPEDGQGSVRLFCGGRFLGVGEWQSGSLRPKRLIATQTGQ
>Mature_300_residues
MQRKIPRRIVDGVLLLDKPSGMTSNGALQTARRLLNAAKAGHTGTLDPMASGLLPLTFGEATKFSQILLDADKTYEAGVK
LGTTTDTGDADGNVVAEHPVSVTREALEEVLSRFRGEIDQLPPMYSALKRDGKPLYEYARAGIEIEREVRRVTIHDLELI
AFSGEHFSMRVRCSKGTYIRTLAMDIGAALGCGAYLDALRRTAIGDFDAARAVTLEALEASPAAMRDGLLEPVDALVAHF
PKVELQPAEAAAILQGRELRKPEDGQGSVRLFCGGRFLGVGEWQSGSLRPKRLIATQTGQ

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=216, Percent_Identity=36.5740740740741, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI215599015, Length=207, Percent_Identity=29.951690821256, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI4503337, Length=207, Percent_Identity=29.951690821256, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI2367200, Length=302, Percent_Identity=47.0198675496689, Blast_Score=265, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17553978, Length=274, Percent_Identity=25.5474452554745, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324037, Length=195, Percent_Identity=33.8461538461538, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323204, Length=273, Percent_Identity=26.7399267399267, Blast_Score=89, Evalue=6e-19,
Organism=Drosophila melanogaster, GI281364189, Length=283, Percent_Identity=26.1484098939929, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364187, Length=283, Percent_Identity=26.1484098939929, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364185, Length=283, Percent_Identity=26.1484098939929, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281364183, Length=283, Percent_Identity=26.1484098939929, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI62471759, Length=283, Percent_Identity=26.1484098939929, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17975520, Length=283, Percent_Identity=26.1484098939929, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_AZOSB (A1K7B7)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_933609.1
- ProteinModelPortal:   A1K7B7
- SMR:   A1K7B7
- STRING:   A1K7B7
- GeneID:   4609818
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo2105
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   A1K7B7
- ProtClustDB:   PRK01851
- BioCyc:   ASP62928:AZO2105-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 32428; Mature: 32428

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: ACT_SITE 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRKIPRRIVDGVLLLDKPSGMTSNGALQTARRLLNAAKAGHTGTLDPMASGLLPLTFGE
CCCCCCHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEECCC
ATKFSQILLDADKTYEAGVKLGTTTDTGDADGNVVAEHPVSVTREALEEVLSRFRGEIDQ
HHHHHHHHHCCCCCHHCCEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHC
LPPMYSALKRDGKPLYEYARAGIEIEREVRRVTIHDLELIAFSGEHFSMRVRCSKGTYIR
CCHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHEEEEEEEECCCCEEEEEEECCCCEEE
TLAMDIGAALGCGAYLDALRRTAIGDFDAARAVTLEALEASPAAMRDGLLEPVDALVAHF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHC
PKVELQPAEAAAILQGRELRKPEDGQGSVRLFCGGRFLGVGEWQSGSLRPKRLIATQTGQ
CCCEECCHHHHHHHCCHHCCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MQRKIPRRIVDGVLLLDKPSGMTSNGALQTARRLLNAAKAGHTGTLDPMASGLLPLTFGE
CCCCCCHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEECCC
ATKFSQILLDADKTYEAGVKLGTTTDTGDADGNVVAEHPVSVTREALEEVLSRFRGEIDQ
HHHHHHHHHCCCCCHHCCEEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHC
LPPMYSALKRDGKPLYEYARAGIEIEREVRRVTIHDLELIAFSGEHFSMRVRCSKGTYIR
CCHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHEEEEEEEECCCCEEEEEEECCCCEEE
TLAMDIGAALGCGAYLDALRRTAIGDFDAARAVTLEALEASPAAMRDGLLEPVDALVAHF
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHC
PKVELQPAEAAAILQGRELRKPEDGQGSVRLFCGGRFLGVGEWQSGSLRPKRLIATQTGQ
CCCEECCHHHHHHHCCHHCCCCCCCCCCEEEEECCEEEECCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA