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The map label for this gene is slt [H]

Identifier: 119898389

GI number: 119898389

Start: 2303193

End: 2303888

Strand: Direct

Name: slt [H]

Synonym: azo2098

Alternate gene names: 119898389

Gene position: 2303193-2303888 (Clockwise)

Preceding gene: 119898374

Following gene: 119898390

Centisome position: 52.63

GC content: 71.84

Gene sequence:

>696_bases
ATGTCGGACGCCTTCCCGCCCCCATCACCGCCGCTGCCCGTGCTTCGATCCGCCCTCGCCGTCATCCTGTGCTCGCTGTC
GTTTTCTGCCGTTGCGGATGGCCTCTACGGCTTCGTCGATGACGCGGGTGTGGCGCACTTCAGCGATGCGGCGAGCGACC
AGCGCTACCGCCGCCTCTCACTGTCCGGGTCGCCAGCCCTGGCCGCCCCCTCACGACGCGCCGCCCCCGCGACGATCGCC
CCCGCGACGATCGCCCCCGCCGCGCTGCGCGACCGCGTGGTGCGCATCGCCGCTGAACACGGCATTGCCCCGGCGCTCGC
CCTGGCGCTGGTGGAGGTGGAAAGCGGGTTCCGCGTCGACGCCCGTTCGCCTAAGGGGGCACTCGGGCTGATGCAGTTGA
TGCCCGCCACCGCGGCGCACTACGGGGTCGGGAACCCGCTCGATGCCGATGCCAATCTGCGCGGCGGGATGCGCTACCTG
TGCGACCTGTTCGCCCTCTTCGGCCGCACCGAGCTGGCGCTGGCGGCCTACAACGCCGGCGAGGGCGCGGTGGCGCGCTA
CGGCAACACGGTGCCGCCCTACCCCGAAACCGTGGCCTATGTGCCCCGCATCCTCGCCCGCCGCGCGGAACTCGCGCGCG
ACCCTGCGATAGGCCTTTCGGACTATCAGGGCCGCCATTCCACCTGCATCCGCTGA

Upstream 100 bases:

>100_bases
ATGACGTTCAACCTACGCCACCCGATGCGGGAAGACGGTTGTCACGGCACCGTCATCCGCGCCGCCTACGCTCGCCCGCG
CCTGACACCGCCTGCGGGCC

Downstream 100 bases:

>100_bases
AACACCGCCCGCCTACAGTGCCGGCGCACCGGGCCCGAGCCCGGGCGACACATCCCGAATATGCGCCGCCCCGCGTGTGC
GGCGCCGACCCTCACAGGAG

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSDAFPPPSPPLPVLRSALAVILCSLSFSAVADGLYGFVDDAGVAHFSDAASDQRYRRLSLSGSPALAAPSRRAAPATIA
PATIAPAALRDRVVRIAAEHGIAPALALALVEVESGFRVDARSPKGALGLMQLMPATAAHYGVGNPLDADANLRGGMRYL
CDLFALFGRTELALAAYNAGEGAVARYGNTVPPYPETVAYVPRILARRAELARDPAIGLSDYQGRHSTCIR

Sequences:

>Translated_231_residues
MSDAFPPPSPPLPVLRSALAVILCSLSFSAVADGLYGFVDDAGVAHFSDAASDQRYRRLSLSGSPALAAPSRRAAPATIA
PATIAPAALRDRVVRIAAEHGIAPALALALVEVESGFRVDARSPKGALGLMQLMPATAAHYGVGNPLDADANLRGGMRYL
CDLFALFGRTELALAAYNAGEGAVARYGNTVPPYPETVAYVPRILARRAELARDPAIGLSDYQGRHSTCIR
>Mature_230_residues
SDAFPPPSPPLPVLRSALAVILCSLSFSAVADGLYGFVDDAGVAHFSDAASDQRYRRLSLSGSPALAAPSRRAAPATIAP
ATIAPAALRDRVVRIAAEHGIAPALALALVEVESGFRVDARSPKGALGLMQLMPATAAHYGVGNPLDADANLRGGMRYLC
DLFALFGRTELALAAYNAGEGAVARYGNTVPPYPETVAYVPRILARRAELARDPAIGLSDYQGRHSTCIR

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=144, Percent_Identity=34.0277777777778, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 24152; Mature: 24021

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAFPPPSPPLPVLRSALAVILCSLSFSAVADGLYGFVDDAGVAHFSDAASDQRYRRLS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEC
LSGSPALAAPSRRAAPATIAPATIAPAALRDRVVRIAAEHGIAPALALALVEVESGFRVD
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEC
ARSPKGALGLMQLMPATAAHYGVGNPLDADANLRGGMRYLCDLFALFGRTELALAAYNAG
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHEEEEECCC
EGAVARYGNTVPPYPETVAYVPRILARRAELARDPAIGLSDYQGRHSTCIR
CCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDAFPPPSPPLPVLRSALAVILCSLSFSAVADGLYGFVDDAGVAHFSDAASDQRYRRLS
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEC
LSGSPALAAPSRRAAPATIAPATIAPAALRDRVVRIAAEHGIAPALALALVEVESGFRVD
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEC
ARSPKGALGLMQLMPATAAHYGVGNPLDADANLRGGMRYLCDLFALFGRTELALAAYNAG
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHEEEEECCC
EGAVARYGNTVPPYPETVAYVPRILARRAELARDPAIGLSDYQGRHSTCIR
CCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]