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The map label for this gene is lexA

Identifier: 119898355

GI number: 119898355

Start: 2264217

End: 2264825

Strand: Direct

Name: lexA

Synonym: azo2064

Alternate gene names: 119898355

Gene position: 2264217-2264825 (Clockwise)

Preceding gene: 119898351

Following gene: 119898356

Centisome position: 51.74

GC content: 65.68

Gene sequence:

>609_bases
ATGAACGCCCGCAACGAACAACTCACGTCCCGCCAGCAGGAGATACTCGACTTCATCCGCCAGACCGTGGAAAGCGAGGG
CCGGCCGCCGACCCGGGCGGAGGTCTGCTCCGCGTTCGGCTTCAAGTCGCCGAATGCGGCTGAAACCCACCTGCGCGCAC
TTGCTGCCAAAGGGGCGATTCTGCTTGAGGAGGGGCGCGCCCGCGGCATCCGCTTGGCCGAGGCGCTCGGGTTGCCGCTG
GTGGGCCGGGTAGCCGCTGGCAATCCCATCTTGGCCGCCGAGCACGTCGAGGCCCGTATCCAGTTCGATCCTGCACTCTT
TTCCCCTCGCGCCGATTATCTGCTGAGGGTGCGCGGCATGAGCATGCGCGACGCGGGCATCCTTGACGGCGATCTCATTG
CCGTCCATCGCTCGCACGAAGCGCGCAACGGACAGGTGGTGGTGGCCCGGATCGATGACGACGTGACGGTGAAGACGCTG
CGCCGGAACGGTCCCATCGTGGAGTTGCTGCCCGCAAATCCGGACTTCGACCCGATCGTCGTCGATACCCGCAGTGCGGC
GCTTGAACTCGAGGGCATCATGGTGGGGCTCATCCGTACCGACCATTGA

Upstream 100 bases:

>100_bases
CGTCTCAAGCCGCACTGCGTCATTCCGTTGTGAGGTGTGCCACGACCGTGGTTGCGGCCAACGGAATACTGTTTGAAAAT
ACAGCAAACAGGAGAAAGCG

Downstream 100 bases:

>100_bases
GCATGCGCAGCTCTTGCACCCGGGCGGATGCCCAGGGGCATTCGTGGCCCGACAGCCGGTGGTCACGCGGCGGGCTTATC
CTCTCTCTGTCATCAACCCT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MNARNEQLTSRQQEILDFIRQTVESEGRPPTRAEVCSAFGFKSPNAAETHLRALAAKGAILLEEGRARGIRLAEALGLPL
VGRVAAGNPILAAEHVEARIQFDPALFSPRADYLLRVRGMSMRDAGILDGDLIAVHRSHEARNGQVVVARIDDDVTVKTL
RRNGPIVELLPANPDFDPIVVDTRSAALELEGIMVGLIRTDH

Sequences:

>Translated_202_residues
MNARNEQLTSRQQEILDFIRQTVESEGRPPTRAEVCSAFGFKSPNAAETHLRALAAKGAILLEEGRARGIRLAEALGLPL
VGRVAAGNPILAAEHVEARIQFDPALFSPRADYLLRVRGMSMRDAGILDGDLIAVHRSHEARNGQVVVARIDDDVTVKTL
RRNGPIVELLPANPDFDPIVVDTRSAALELEGIMVGLIRTDH
>Mature_202_residues
MNARNEQLTSRQQEILDFIRQTVESEGRPPTRAEVCSAFGFKSPNAAETHLRALAAKGAILLEEGRARGIRLAEALGLPL
VGRVAAGNPILAAEHVEARIQFDPALFSPRADYLLRVRGMSMRDAGILDGDLIAVHRSHEARNGQVVVARIDDDVTVKTL
RRNGPIVELLPANPDFDPIVVDTRSAALELEGIMVGLIRTDH

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=195, Percent_Identity=60.5128205128205, Blast_Score=243, Evalue=5e-66,
Organism=Escherichia coli, GI1787431, Length=130, Percent_Identity=35.3846153846154, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_AZOSB (A1K776)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_933568.1
- ProteinModelPortal:   A1K776
- SMR:   A1K776
- STRING:   A1K776
- MEROPS:   S24.001
- GeneID:   4608379
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo2064
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   IAENPDF
- PhylomeDB:   A1K776
- ProtClustDB:   PRK00215
- BioCyc:   ASP62928:AZO2064-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 22024; Mature: 22024

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: ACT_SITE 121-121 ACT_SITE 158-158

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARNEQLTSRQQEILDFIRQTVESEGRPPTRAEVCSAFGFKSPNAAETHLRALAAKGAI
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCEE
LLEEGRARGIRLAEALGLPLVGRVAAGNPILAAEHVEARIQFDPALFSPRADYLLRVRGM
EEECCCCCCEEHHHHHCCCCEEEECCCCCEEEECCEEEEEEECCCCCCCCCCEEEEECCC
SMRDAGILDGDLIAVHRSHEARNGQVVVARIDDDVTVKTLRRNGPIVELLPANPDFDPIV
CCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCCEEEEECCCCCCCEEE
VDTRSAALELEGIMVGLIRTDH
EECCCCEEEECCEEEEEEEECC
>Mature Secondary Structure
MNARNEQLTSRQQEILDFIRQTVESEGRPPTRAEVCSAFGFKSPNAAETHLRALAAKGAI
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHCCEE
LLEEGRARGIRLAEALGLPLVGRVAAGNPILAAEHVEARIQFDPALFSPRADYLLRVRGM
EEECCCCCCEEHHHHHCCCCEEEECCCCCEEEECCEEEEEEECCCCCCCCCCEEEEECCC
SMRDAGILDGDLIAVHRSHEARNGQVVVARIDDDVTVKTLRRNGPIVELLPANPDFDPIV
CCCCCCCCCCCEEEEECCCCCCCCCEEEEEECCCEEEEEECCCCCEEEEECCCCCCCEEE
VDTRSAALELEGIMVGLIRTDH
EECCCCEEEECCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA