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The map label for this gene is trmJ [H]

Identifier: 119898311

GI number: 119898311

Start: 2223803

End: 2224531

Strand: Reverse

Name: trmJ [H]

Synonym: azo2020

Alternate gene names: 119898311

Gene position: 2224531-2223803 (Counterclockwise)

Preceding gene: 119898313

Following gene: 119898310

Centisome position: 50.83

GC content: 67.9

Gene sequence:

>729_bases
ATGAACGCCGCCCTCGCGCTCGACCGCGTCCGCATTGTGCTGTCGCGCACCAGTCATCCCGGAAATATAGGCGCTGCCGC
GCGCGCAATGAAGACCATGGGGCTGTCCCAGCTGTGGCTGGTCGCGCCCGAGTCATTCCCCGACCCGGTGGCGGACGCAC
GGGCATCGGGCGCGGACGACCTGCTGCGCAATGCGCGGGTGGTCGGCAGTCTGGCGGAAGCGCTCGCCGGCACCGTGCTG
GCCGCTGCGGTCACCGCCCGCCGGCGCGAACTTGCGCTACCACGGCGTGACGCGCGCGCGGCTGCCGTGGAACTGCTGCG
CTTTGCCGAGCAAGGGGACGTGGCGCTGGTGTTCGGCAACGAAACCAGCGGCCTGTCCAACGAAGACGTGCTGCTGTGTG
GCATGCCGGTTTCCATCCCCGCCAATCCCGCGTTCTCCTCGCTCAATCTCGGCGCCGCGGTCCAGCTGCTGTGCTACGAG
ATGCGGATGGCGGCGTTGCAGCCGCAACTGGATGCAGACCCGGCACCGCGGCTGGCAACGCATGACGAGGTGGAGGGCTT
CCTGCAGCACCTCGAGCAGGCGGTGACCGAGAGCGGCTTTCACGAACCGTCCAATCCCCGCCGGCTGTTGCCGCGACTGC
GTCGGCTGTTCGGCCGCGTCCGCCTGGAAAAGGAAGAGGTCGGCATCCTGCGCGGGATGCTCTCTAGCTTTCAGCGAAAA
GTCGATTAA

Upstream 100 bases:

>100_bases
ATCGGATGCATTCAGGTGTGTCTCGGCAGTGGGGGTAGGGCGGGACGCGCGATGCATCCGGCCCGGCCGCAATCAGGTAC
GAAAAACGCAGAATTCTATT

Downstream 100 bases:

>100_bases
AATAGTCGGAAATTAAACCGACAACGTGGGCGTGCGCGCGACATTGTCACGCCCTGCCGTCCACCCTTTCTGGCAAGGAA
AACAGCATGTTCAGCCACCT

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MNAALALDRVRIVLSRTSHPGNIGAAARAMKTMGLSQLWLVAPESFPDPVADARASGADDLLRNARVVGSLAEALAGTVL
AAAVTARRRELALPRRDARAAAVELLRFAEQGDVALVFGNETSGLSNEDVLLCGMPVSIPANPAFSSLNLGAAVQLLCYE
MRMAALQPQLDADPAPRLATHDEVEGFLQHLEQAVTESGFHEPSNPRRLLPRLRRLFGRVRLEKEEVGILRGMLSSFQRK
VD

Sequences:

>Translated_242_residues
MNAALALDRVRIVLSRTSHPGNIGAAARAMKTMGLSQLWLVAPESFPDPVADARASGADDLLRNARVVGSLAEALAGTVL
AAAVTARRRELALPRRDARAAAVELLRFAEQGDVALVFGNETSGLSNEDVLLCGMPVSIPANPAFSSLNLGAAVQLLCYE
MRMAALQPQLDADPAPRLATHDEVEGFLQHLEQAVTESGFHEPSNPRRLLPRLRRLFGRVRLEKEEVGILRGMLSSFQRK
VD
>Mature_242_residues
MNAALALDRVRIVLSRTSHPGNIGAAARAMKTMGLSQLWLVAPESFPDPVADARASGADDLLRNARVVGSLAEALAGTVL
AAAVTARRRELALPRRDARAAAVELLRFAEQGDVALVFGNETSGLSNEDVLLCGMPVSIPANPAFSSLNLGAAVQLLCYE
MRMAALQPQLDADPAPRLATHDEVEGFLQHLEQAVTESGFHEPSNPRRLLPRLRRLFGRVRLEKEEVGILRGMLSSFQRK
VD

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=241, Percent_Identity=45.643153526971, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1790865, Length=239, Percent_Identity=34.3096234309623, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26105; Mature: 26105

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAALALDRVRIVLSRTSHPGNIGAAARAMKTMGLSQLWLVAPESFPDPVADARASGADD
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHEEEECCCCCCCCHHHHHCCCHHH
LLRNARVVGSLAEALAGTVLAAAVTARRRELALPRRDARAAAVELLRFAEQGDVALVFGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECC
ETSGLSNEDVLLCGMPVSIPANPAFSSLNLGAAVQLLCYEMRMAALQPQLDADPAPRLAT
CCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
HDEVEGFLQHLEQAVTESGFHEPSNPRRLLPRLRRLFGRVRLEKEEVGILRGMLSSFQRK
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VD
CC
>Mature Secondary Structure
MNAALALDRVRIVLSRTSHPGNIGAAARAMKTMGLSQLWLVAPESFPDPVADARASGADD
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHEEEECCCCCCCCHHHHHCCCHHH
LLRNARVVGSLAEALAGTVLAAAVTARRRELALPRRDARAAAVELLRFAEQGDVALVFGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEECC
ETSGLSNEDVLLCGMPVSIPANPAFSSLNLGAAVQLLCYEMRMAALQPQLDADPAPRLAT
CCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
HDEVEGFLQHLEQAVTESGFHEPSNPRRLLPRLRRLFGRVRLEKEEVGILRGMLSSFQRK
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VD
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA