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The map label for this gene is iscS1 [H]

Identifier: 119898307

GI number: 119898307

Start: 2219958

End: 2221169

Strand: Reverse

Name: iscS1 [H]

Synonym: azo2016

Alternate gene names: 119898307

Gene position: 2221169-2219958 (Counterclockwise)

Preceding gene: 119898308

Following gene: 119898306

Centisome position: 50.76

GC content: 63.53

Gene sequence:

>1212_bases
ATGTTGAAGTTTCCGATCTACCTCGACTACTCGGCGACGACGCCGGTCGACCCGCGCGTGGCGGCGAAGATGATTCCGTG
GCTGACCGAACACTTCGGCAACCCGGCGAGCCGTTCGCACGCCTTTGGCTGGGAGGCGGAGCGCGCGGTTGAAGAGGCGC
GCGACGAGGTGGCGAAGTTGGTGAACGCCGACCCGAAGGAAATCGTGTGGACCTCCGGGGCGACCGAGTCGAACAATCTC
GCGATCAAGGGCGCCGCGCACTTTTACCGCAGCAAGGGCAAGCACGTCATCACCCTCAAGACCGAGCACAAGGCGGTGCT
GGATACGGTGCGCGAACTCGAGCGCGAAGGCTTCGAGGCCACCTACCTGGACGTCCAGGAAAACGGCCTGATCGACCTGG
AAGCCTTCAAGGCGGCGCTGCGCCCGGACACCACCGTGGTGTCGGTGATGTTCGTCAACAACGAAATCGGTGTGATCCAG
CCGATCGCCGAGATCGGCGAGATTTGCCGCGAAAAGGGCATCGTGTTTCATGTGGACGCCGCCCAGGCTACCGGCAAGGT
CGAGATCGACCTTGCCAAGCTCAAGGTGGACCTGATGAGCTTTTCGGCACACAAGACCTACGGCCCGAAAGGCATCGGCG
CGCTGTACGTGCGCCGCAAGCCGCGGGTGCGTCTGGAGGCGCAGATGCATGGCGGCGGCCACGAGCGCGGCCTGCGCTCG
GGCACGCTGGCCACGCACCAGATCGTCGGCATGGGCGAGGCCTTCCGCATCGCCCGCGAAGAAATGGCCGAGGAGAACGG
CCGTATCCGCCGCCTGCGCGACAAGCTGTTGAACGGCCTGACCGATATCGACGCGACCTACGTCAATGGCGATGTGGAGC
ACCGCGTGCCGCACAACCTCAACATCTCGTTCGCCTACGTCGAGGGCGAATCCTTGATCATGGCGATCAAGGACATCGCG
GTGTCGTCCGGCTCGGCCTGTACGTCTGCGAGCCTGGAGCCGTCCTACGTGCTGCGGGCGCTCGGTCGTGACGATGAACT
CGCCCACAGCTCGATCCGCTTCACGATCGGCCGCTTCACCACCGAAGAAGAAATCGACTACGCCATTGATCTGCTCCACA
AGAAGATCGGCAAGCTGCGCGAACTCTCCCCGCTGTGGGAGATGGTGCAGGAGGGCGTGGATTTGAACACCGTGCAGTGG
GCCGCTCACTAA

Upstream 100 bases:

>100_bases
TGCCTTCCTGGCAACGCTGGGCGTCCTTGCGGCAGAACTGAGACAAATGACCGCGCTTGCGGTCTGACATGAAAAGACGA
ATCGCTAACGGAGTGACCCC

Downstream 100 bases:

>100_bases
GCCGCCCGCAACCCCAAGCAGGAGAAGCAAAATGGCATACAGCGACAAACTTCTGGACCACTACGAAAACCCCCGCAACG
TCGGCTCCTTCGGCAAGGAC

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MLKFPIYLDYSATTPVDPRVAAKMIPWLTEHFGNPASRSHAFGWEAERAVEEARDEVAKLVNADPKEIVWTSGATESNNL
AIKGAAHFYRSKGKHVITLKTEHKAVLDTVRELEREGFEATYLDVQENGLIDLEAFKAALRPDTTVVSVMFVNNEIGVIQ
PIAEIGEICREKGIVFHVDAAQATGKVEIDLAKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRLEAQMHGGGHERGLRS
GTLATHQIVGMGEAFRIAREEMAEENGRIRRLRDKLLNGLTDIDATYVNGDVEHRVPHNLNISFAYVEGESLIMAIKDIA
VSSGSACTSASLEPSYVLRALGRDDELAHSSIRFTIGRFTTEEEIDYAIDLLHKKIGKLRELSPLWEMVQEGVDLNTVQW
AAH

Sequences:

>Translated_403_residues
MLKFPIYLDYSATTPVDPRVAAKMIPWLTEHFGNPASRSHAFGWEAERAVEEARDEVAKLVNADPKEIVWTSGATESNNL
AIKGAAHFYRSKGKHVITLKTEHKAVLDTVRELEREGFEATYLDVQENGLIDLEAFKAALRPDTTVVSVMFVNNEIGVIQ
PIAEIGEICREKGIVFHVDAAQATGKVEIDLAKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRLEAQMHGGGHERGLRS
GTLATHQIVGMGEAFRIAREEMAEENGRIRRLRDKLLNGLTDIDATYVNGDVEHRVPHNLNISFAYVEGESLIMAIKDIA
VSSGSACTSASLEPSYVLRALGRDDELAHSSIRFTIGRFTTEEEIDYAIDLLHKKIGKLRELSPLWEMVQEGVDLNTVQW
AAH
>Mature_403_residues
MLKFPIYLDYSATTPVDPRVAAKMIPWLTEHFGNPASRSHAFGWEAERAVEEARDEVAKLVNADPKEIVWTSGATESNNL
AIKGAAHFYRSKGKHVITLKTEHKAVLDTVRELEREGFEATYLDVQENGLIDLEAFKAALRPDTTVVSVMFVNNEIGVIQ
PIAEIGEICREKGIVFHVDAAQATGKVEIDLAKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRLEAQMHGGGHERGLRS
GTLATHQIVGMGEAFRIAREEMAEENGRIRRLRDKLLNGLTDIDATYVNGDVEHRVPHNLNISFAYVEGESLIMAIKDIA
VSSGSACTSASLEPSYVLRALGRDDELAHSSIRFTIGRFTTEEEIDYAIDLLHKKIGKLRELSPLWEMVQEGVDLNTVQW
AAH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=400, Percent_Identity=60, Blast_Score=535, Evalue=1e-152,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=32.1256038647343, Blast_Score=195, Evalue=6e-50,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=72.7722772277228, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1789175, Length=232, Percent_Identity=29.7413793103448, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1787970, Length=181, Percent_Identity=29.8342541436464, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=401, Percent_Identity=61.3466334164589, Blast_Score=535, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17533177, Length=327, Percent_Identity=33.0275229357798, Blast_Score=157, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6319831, Length=399, Percent_Identity=61.1528822055138, Blast_Score=530, Evalue=1e-151,
Organism=Drosophila melanogaster, GI20129463, Length=400, Percent_Identity=59.5, Blast_Score=504, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44781; Mature: 44781

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFPIYLDYSATTPVDPRVAAKMIPWLTEHFGNPASRSHAFGWEAERAVEEARDEVAKL
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VNADPKEIVWTSGATESNNLAIKGAAHFYRSKGKHVITLKTEHKAVLDTVRELEREGFEA
HCCCCHHEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCE
TYLDVQENGLIDLEAFKAALRPDTTVVSVMFVNNEIGVIQPIAEIGEICREKGIVFHVDA
EEEEECCCCEEEHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEC
AQATGKVEIDLAKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRLEAQMHGGGHERGLRS
CCCCCEEEEEHHHEEEEHHHCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCC
GTLATHQIVGMGEAFRIAREEMAEENGRIRRLRDKLLNGLTDIDATYVNGDVEHRVPHNL
CCCHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
NISFAYVEGESLIMAIKDIAVSSGSACTSASLEPSYVLRALGRDDELAHSSIRFTIGRFT
CEEEEEEECCEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCEEEEEEECCC
TEEEIDYAIDLLHKKIGKLRELSPLWEMVQEGVDLNTVQWAAH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure
MLKFPIYLDYSATTPVDPRVAAKMIPWLTEHFGNPASRSHAFGWEAERAVEEARDEVAKL
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VNADPKEIVWTSGATESNNLAIKGAAHFYRSKGKHVITLKTEHKAVLDTVRELEREGFEA
HCCCCHHEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCE
TYLDVQENGLIDLEAFKAALRPDTTVVSVMFVNNEIGVIQPIAEIGEICREKGIVFHVDA
EEEEECCCCEEEHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEC
AQATGKVEIDLAKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRLEAQMHGGGHERGLRS
CCCCCEEEEEHHHEEEEHHHCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCC
GTLATHQIVGMGEAFRIAREEMAEENGRIRRLRDKLLNGLTDIDATYVNGDVEHRVPHNL
CCCHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCC
NISFAYVEGESLIMAIKDIAVSSGSACTSASLEPSYVLRALGRDDELAHSSIRFTIGRFT
CEEEEEEECCEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCEEEEEEECCC
TEEEIDYAIDLLHKKIGKLRELSPLWEMVQEGVDLNTVQWAAH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA