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The map label for this gene is yfhF [H]

Identifier: 119898305

GI number: 119898305

Start: 2219199

End: 2219522

Strand: Reverse

Name: yfhF [H]

Synonym: azo2014

Alternate gene names: 119898305

Gene position: 2219522-2219199 (Counterclockwise)

Preceding gene: 119898306

Following gene: 119898304

Centisome position: 50.72

GC content: 60.8

Gene sequence:

>324_bases
ATGGGAGTGACGCTTTCTCAAAGCGCGGCCAAGCACGTCGCGAATTTCATCGCCAAGCGTGGCAAGGGCCTGGGAATCCG
TCTCGGGGTGCGTACCTCGGGCTGTTCCGGCATGGCCTACAAGCTGGAGTTCGTCGATGAGAAGCAGGACGAGGACCTGG
TCTTCGAGAGCCACGGCGTGCAGGTGATCGTCGATCCGAAGAGCCTGCCCTACATCGACGGCACCGAGCTGGACTTCGTG
CGCGAGGGCCTGAACGAAGGCTTCAAGTTCAACAACCCGAACGTGAAAGACGCCTGCGGTTGCGGCGAGAGCTTCAACGT
CTGA

Upstream 100 bases:

>100_bases
TGAAGATCCATTGTTCCATCCTGGCCGAAGACGCGATCAAGGCCGCGGTGGCGGACTACAAGAAGAAGCACGAAGCCTGA
CCATCACAGGAGAGTCGAGC

Downstream 100 bases:

>100_bases
ACGCATGGCCATCGATCTGCAGCAGGACTACTTCTCGCTGTTCGGGATGCCGCGCCGCTTTCGCATCGACGAGTCGGCGC
TGGAGGCGGCGTGGCACGGC

Product: putative regulatory protein

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 107; Mature: 106

Protein sequence:

>107_residues
MGVTLSQSAAKHVANFIAKRGKGLGIRLGVRTSGCSGMAYKLEFVDEKQDEDLVFESHGVQVIVDPKSLPYIDGTELDFV
REGLNEGFKFNNPNVKDACGCGESFNV

Sequences:

>Translated_107_residues
MGVTLSQSAAKHVANFIAKRGKGLGIRLGVRTSGCSGMAYKLEFVDEKQDEDLVFESHGVQVIVDPKSLPYIDGTELDFV
REGLNEGFKFNNPNVKDACGCGESFNV
>Mature_106_residues
GVTLSQSAAKHVANFIAKRGKGLGIRLGVRTSGCSGMAYKLEFVDEKQDEDLVFESHGVQVIVDPKSLPYIDGTELDFVR
EGLNEGFKFNNPNVKDACGCGESFNV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=40.5660377358491, Blast_Score=98, Evalue=2e-21,
Organism=Homo sapiens, GI160420328, Length=83, Percent_Identity=38.5542168674699, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=72.8971962616822, Blast_Score=163, Evalue=2e-42,
Organism=Escherichia coli, GI1787974, Length=106, Percent_Identity=50, Blast_Score=119, Evalue=3e-29,
Organism=Escherichia coli, GI1786351, Length=106, Percent_Identity=39.622641509434, Blast_Score=74, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=36.7924528301887, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25146411, Length=106, Percent_Identity=36.7924528301887, Blast_Score=92, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323001, Length=105, Percent_Identity=42.8571428571429, Blast_Score=91, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24642263, Length=106, Percent_Identity=37.7358490566038, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 11599; Mature: 11468

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVTLSQSAAKHVANFIAKRGKGLGIRLGVRTSGCSGMAYKLEFVDEKQDEDLVFESHGV
CCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCEEEECCCC
QVIVDPKSLPYIDGTELDFVREGLNEGFKFNNPNVKDACGCGESFNV
EEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GVTLSQSAAKHVANFIAKRGKGLGIRLGVRTSGCSGMAYKLEFVDEKQDEDLVFESHGV
CCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCEEEECCCC
QVIVDPKSLPYIDGTELDFVREGLNEGFKFNNPNVKDACGCGESFNV
EEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA