Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 119898292

GI number: 119898292

Start: 2203255

End: 2205336

Strand: Reverse

Name: ligA

Synonym: azo2001

Alternate gene names: 119898292

Gene position: 2205336-2203255 (Counterclockwise)

Preceding gene: 119898293

Following gene: 119898291

Centisome position: 50.4

GC content: 67.82

Gene sequence:

>2082_bases
ATGCCGCTGTTTGCCGATGCTGCCGCGCGGGCGGCGACCCTGCGGGCGGAAATCGCCCGCCATAACCACGCCTACTACGT
CCTCGACACGCCGACGATTCCGGACGCCGAGTACGACCGCCTGTTCCGCGAACTGCAGGATATCGAGGCGGCGCACCCCG
AGTTGCTCACGCCCGATTCGCCCACCCAGCGGGTCGGCGGCGCGCCGCTTCCTGAACTGGTGCCGGTGCGCCATGCGGTG
CCGATGTTGTCCATCCGCACGGAGACCGACACCACTGCGCAAGGCGTCCGCAACTTCGACACCCGGGTGCGAAACGCGCT
CGGCTTGGGCGATGGCGACCCTGCAGTGGAATACCTGAGCGAACTCAAGTTCGACGGTCTCGCCATCAGCCTGCGTTACG
AACACGGCGTGCTGGTTCGGGCAGCCACGCGCGGCGATGGCGAAACCGGCGAGGACGTCACCCACAACGTGCGCACGATC
CAGCAGATTCCGCTACGCCTGCACGGGGCGGCCCCTGCGGTCATCGAGGTCCGCGGCGAGATCTACATGCGGCGCGACGA
CTTCGAAGCACTCAACAACCGCCAGCGCGAAGCGGGCGACAAGGTCTTCGTCAACCCGCGCAACGCCGCGGCCGGCGCCG
TACGCCAGCTTGATCCGCGCATCGCCGCGCGCCGTCCCTTGTCCTTCTTCGCCTACGGGCTGGGGGAGCATGCCGGATTC
GATCTGCCCGCTACGCAGGCGGAACTATTGACCCGCCTCGCCGCGTTTGGCGTGCCGGTATGCGAACACCGGGAGGTCTC
CTCGGGCCCGGAGGGCCTGATTGCCTTCCACGACCGCATCGCCGCGTTGCGCAACGAACTGCCCTACGACATCGACGGCG
TGGTCTACAAGGTGAACCGCGTCGACCTGCAGCGCGAACTCGGTTTCGTCACCCGCGAGCCGCGCTGGGCGGTGGCGCAC
AAGTACCCGGCGCAGGAAGAGATCACGCGCCTCGCCGGCATCGACGTGCAGGTTGGCCGCACCGGCGCACTCACGCCGGT
GGCAAGGCTGGAGCCGGTCTTCGTCGGCGGCGTCACGGTTACCAACGCAACGCTGCACAACCAGGACGAGATCGACCGCA
AGGACGTGCGCGTCGGCGACTGGGTGATCGTGCGCCGCGCGGGCGACGTGATTCCCGAAGTTGTCGGCCCGGTGCTGGAG
CGCCGCGTAGGCGAACCGCCGCGCTTCAACCTGCTGGCGAGCTATCCGACCTGCCCGGTGTGCGGCTCGCACGTGGTGCG
CGGCGAGGACGAGGCGGTGGCGCGCTGCAGCGGCGGCCTGTTCTGCCCGGCGCAGCGCAAGCAGGCGCTGTTGCATTTCG
CGGGCCGCCGCGCGATGGACATCGAAGGACTCGGCGACAAACTGGTCGAGCAACTCGTCGATGCCAGCATCGTGAAGACG
CCGGCCGACCTCTACAAGCTCGGCATCCTCGCGCTCGCCAATCTGGAGCGCATGGGTGAGAAGTCCGCGCAGAACCTGTT
GGCGGCCATCGAGAAGAGCCGCAATACCACGCTCGCCCGCTTCATCTTCGCGCTCGGCATCCGCAACGTCGGCGAAGCCA
CCGCGAAGGATCTCGCCCGCCATTTCGGCAGCCTCGATGCATTGCTGGCGGCGGACGAGGGCGCGCTGCAACAAGTGCCG
GACGTCGGCCCGGTGGTCGCAAAGTGCATTGCCGAGTTTTTCGCCGAGGCCCACAACGTCGAGGTGGTCGAGCAACTGCG
CGCGGCCGGCCTGCGTTGGGAGGAGGGCGCGCCTGCGGCGCCGGCCAGCGGCAGCGTCGCAGGCCTGACCTTCGTAATCA
CCGGCACGCTGCCGACACTGTCGCGCGACGAGGCCAAGGCGCTGATCGAGGCCCACGGCGGCAAGGTCAGTGGTTCGGTT
TCCAAGAAGACGGATTACCTGGTGGCGGGGGCCGAAGCCGGTTCCAAGCTCGCCAAGGCGCAGGATCTCGGGGTGGACAT
CGTCGATGAGGACGGTCTGCGCCGATTGCTGGAATCGGGCGAACAGTCGCCCATCAACAACAAGGACGGAGTCCCAGAAT
GA

Upstream 100 bases:

>100_bases
ATGATCCGCAGCCAGATCGCGCAGTTCCAGGACCGCATGGCCAGCCACGACATCCCCGCCGGCGGCCCGCTCGCTCTCCG
CCTTTTCGCTGCCTGACACG

Downstream 100 bases:

>100_bases
AAAAGGTCACCAAGGCGGTCTTTCCGGTTGCGGGCATGGGCACGCGCTTCCTGCCGGCCACCAAGGCCAGTCCGAAGGAA
ATGCTGCCGGTCGTCGACAA

Product: DNA ligase (NAD+)

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 693; Mature: 692

Protein sequence:

>693_residues
MPLFADAAARAATLRAEIARHNHAYYVLDTPTIPDAEYDRLFRELQDIEAAHPELLTPDSPTQRVGGAPLPELVPVRHAV
PMLSIRTETDTTAQGVRNFDTRVRNALGLGDGDPAVEYLSELKFDGLAISLRYEHGVLVRAATRGDGETGEDVTHNVRTI
QQIPLRLHGAAPAVIEVRGEIYMRRDDFEALNNRQREAGDKVFVNPRNAAAGAVRQLDPRIAARRPLSFFAYGLGEHAGF
DLPATQAELLTRLAAFGVPVCEHREVSSGPEGLIAFHDRIAALRNELPYDIDGVVYKVNRVDLQRELGFVTREPRWAVAH
KYPAQEEITRLAGIDVQVGRTGALTPVARLEPVFVGGVTVTNATLHNQDEIDRKDVRVGDWVIVRRAGDVIPEVVGPVLE
RRVGEPPRFNLLASYPTCPVCGSHVVRGEDEAVARCSGGLFCPAQRKQALLHFAGRRAMDIEGLGDKLVEQLVDASIVKT
PADLYKLGILALANLERMGEKSAQNLLAAIEKSRNTTLARFIFALGIRNVGEATAKDLARHFGSLDALLAADEGALQQVP
DVGPVVAKCIAEFFAEAHNVEVVEQLRAAGLRWEEGAPAAPASGSVAGLTFVITGTLPTLSRDEAKALIEAHGGKVSGSV
SKKTDYLVAGAEAGSKLAKAQDLGVDIVDEDGLRRLLESGEQSPINNKDGVPE

Sequences:

>Translated_693_residues
MPLFADAAARAATLRAEIARHNHAYYVLDTPTIPDAEYDRLFRELQDIEAAHPELLTPDSPTQRVGGAPLPELVPVRHAV
PMLSIRTETDTTAQGVRNFDTRVRNALGLGDGDPAVEYLSELKFDGLAISLRYEHGVLVRAATRGDGETGEDVTHNVRTI
QQIPLRLHGAAPAVIEVRGEIYMRRDDFEALNNRQREAGDKVFVNPRNAAAGAVRQLDPRIAARRPLSFFAYGLGEHAGF
DLPATQAELLTRLAAFGVPVCEHREVSSGPEGLIAFHDRIAALRNELPYDIDGVVYKVNRVDLQRELGFVTREPRWAVAH
KYPAQEEITRLAGIDVQVGRTGALTPVARLEPVFVGGVTVTNATLHNQDEIDRKDVRVGDWVIVRRAGDVIPEVVGPVLE
RRVGEPPRFNLLASYPTCPVCGSHVVRGEDEAVARCSGGLFCPAQRKQALLHFAGRRAMDIEGLGDKLVEQLVDASIVKT
PADLYKLGILALANLERMGEKSAQNLLAAIEKSRNTTLARFIFALGIRNVGEATAKDLARHFGSLDALLAADEGALQQVP
DVGPVVAKCIAEFFAEAHNVEVVEQLRAAGLRWEEGAPAAPASGSVAGLTFVITGTLPTLSRDEAKALIEAHGGKVSGSV
SKKTDYLVAGAEAGSKLAKAQDLGVDIVDEDGLRRLLESGEQSPINNKDGVPE
>Mature_692_residues
PLFADAAARAATLRAEIARHNHAYYVLDTPTIPDAEYDRLFRELQDIEAAHPELLTPDSPTQRVGGAPLPELVPVRHAVP
MLSIRTETDTTAQGVRNFDTRVRNALGLGDGDPAVEYLSELKFDGLAISLRYEHGVLVRAATRGDGETGEDVTHNVRTIQ
QIPLRLHGAAPAVIEVRGEIYMRRDDFEALNNRQREAGDKVFVNPRNAAAGAVRQLDPRIAARRPLSFFAYGLGEHAGFD
LPATQAELLTRLAAFGVPVCEHREVSSGPEGLIAFHDRIAALRNELPYDIDGVVYKVNRVDLQRELGFVTREPRWAVAHK
YPAQEEITRLAGIDVQVGRTGALTPVARLEPVFVGGVTVTNATLHNQDEIDRKDVRVGDWVIVRRAGDVIPEVVGPVLER
RVGEPPRFNLLASYPTCPVCGSHVVRGEDEAVARCSGGLFCPAQRKQALLHFAGRRAMDIEGLGDKLVEQLVDASIVKTP
ADLYKLGILALANLERMGEKSAQNLLAAIEKSRNTTLARFIFALGIRNVGEATAKDLARHFGSLDALLAADEGALQQVPD
VGPVVAKCIAEFFAEAHNVEVVEQLRAAGLRWEEGAPAAPASGSVAGLTFVITGTLPTLSRDEAKALIEAHGGKVSGSVS
KKTDYLVAGAEAGSKLAKAQDLGVDIVDEDGLRRLLESGEQSPINNKDGVPE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=77, Percent_Identity=58.4415584415584, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1788750, Length=672, Percent_Identity=54.6130952380952, Blast_Score=697, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=592, Percent_Identity=23.8175675675676, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324791, Length=129, Percent_Identity=34.1085271317829, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI62472391, Length=73, Percent_Identity=50.6849315068493, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17737765, Length=73, Percent_Identity=50.6849315068493, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_AZOSB (A1K713)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_933505.1
- ProteinModelPortal:   A1K713
- SMR:   A1K713
- STRING:   A1K713
- GeneID:   4608857
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo2001
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   A1K713
- ProtClustDB:   CLSK767052
- BioCyc:   ASP62928:AZO2001-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74908; Mature: 74777

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 123-123 BINDING 121-121 BINDING 144-144 BINDING 180-180 BINDING 297-297 BINDING 321-321

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLFADAAARAATLRAEIARHNHAYYVLDTPTIPDAEYDRLFRELQDIEAAHPELLTPDS
CCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
PTQRVGGAPLPELVPVRHAVPMLSIRTETDTTAQGVRNFDTRVRNALGLGDGDPAVEYLS
CHHHCCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ELKFDGLAISLRYEHGVLVRAATRGDGETGEDVTHNVRTIQQIPLRLHGAAPAVIEVRGE
HCCCCCEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCEECCCCCEEEEECCE
IYMRRDDFEALNNRQREAGDKVFVNPRNAAAGAVRQLDPRIAARRPLSFFAYGLGEHAGF
EEEECCHHHHHHCHHHHCCCEEEECCCCHHHHHHHHCCCHHHHCCCHHHHHHHCCCCCCC
DLPATQAELLTRLAAFGVPVCEHREVSSGPEGLIAFHDRIAALRNELPYDIDGVVYKVNR
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHH
VDLQRELGFVTREPRWAVAHKYPAQEEITRLAGIDVQVGRTGALTPVARLEPVFVGGVTV
HHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCEEEECCCCCCCHHHHCCEEEECCEEE
TNATLHNQDEIDRKDVRVGDWVIVRRAGDVIPEVVGPVLERRVGEPPRFNLLASYPTCPV
EECEECCCCCCCHHHCEECCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
CGSHVVRGEDEAVARCSGGLFCPAQRKQALLHFAGRRAMDIEGLGDKLVEQLVDASIVKT
CCCCEECCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
PADLYKLGILALANLERMGEKSAQNLLAAIEKSRNTTLARFIFALGIRNVGEATAKDLAR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHH
HFGSLDALLAADEGALQQVPDVGPVVAKCIAEFFAEAHNVEVVEQLRAAGLRWEEGAPAA
HHCCHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCC
PASGSVAGLTFVITGTLPTLSRDEAKALIEAHGGKVSGSVSKKTDYLVAGAEAGSKLAKA
CCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCEECCCCCCCCCEEEECCCCCHHHHHH
QDLGVDIVDEDGLRRLLESGEQSPINNKDGVPE
HHCCCEEECHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PLFADAAARAATLRAEIARHNHAYYVLDTPTIPDAEYDRLFRELQDIEAAHPELLTPDS
CCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
PTQRVGGAPLPELVPVRHAVPMLSIRTETDTTAQGVRNFDTRVRNALGLGDGDPAVEYLS
CHHHCCCCCCCHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
ELKFDGLAISLRYEHGVLVRAATRGDGETGEDVTHNVRTIQQIPLRLHGAAPAVIEVRGE
HCCCCCEEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCEECCCCCEEEEECCE
IYMRRDDFEALNNRQREAGDKVFVNPRNAAAGAVRQLDPRIAARRPLSFFAYGLGEHAGF
EEEECCHHHHHHCHHHHCCCEEEECCCCHHHHHHHHCCCHHHHCCCHHHHHHHCCCCCCC
DLPATQAELLTRLAAFGVPVCEHREVSSGPEGLIAFHDRIAALRNELPYDIDGVVYKVNR
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHH
VDLQRELGFVTREPRWAVAHKYPAQEEITRLAGIDVQVGRTGALTPVARLEPVFVGGVTV
HHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCEEEECCCCCCCHHHHCCEEEECCEEE
TNATLHNQDEIDRKDVRVGDWVIVRRAGDVIPEVVGPVLERRVGEPPRFNLLASYPTCPV
EECEECCCCCCCHHHCEECCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
CGSHVVRGEDEAVARCSGGLFCPAQRKQALLHFAGRRAMDIEGLGDKLVEQLVDASIVKT
CCCCEECCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
PADLYKLGILALANLERMGEKSAQNLLAAIEKSRNTTLARFIFALGIRNVGEATAKDLAR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHH
HFGSLDALLAADEGALQQVPDVGPVVAKCIAEFFAEAHNVEVVEQLRAAGLRWEEGAPAA
HHCCHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCC
PASGSVAGLTFVITGTLPTLSRDEAKALIEAHGGKVSGSVSKKTDYLVAGAEAGSKLAKA
CCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCEECCCCCCCCCEEEECCCCCHHHHHH
QDLGVDIVDEDGLRRLLESGEQSPINNKDGVPE
HHCCCEEECHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA