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The map label for this gene is xylF [H]

Identifier: 119898259

GI number: 119898259

Start: 2165047

End: 2165901

Strand: Reverse

Name: xylF [H]

Synonym: azo1968

Alternate gene names: 119898259

Gene position: 2165901-2165047 (Counterclockwise)

Preceding gene: 119898261

Following gene: 119898258

Centisome position: 49.49

GC content: 67.72

Gene sequence:

>855_bases
ATGAGCCAGAACAATCCCGAACTGGGATCGAGCATCGAGATCGGTTCGGTCCGCACCAACTACCACGACCTGGGCAAAGG
CTTTCCGGTGCTGATGATCCACGGCTCCGGCCCCGGCGTCAGCGGCTACGTCAATTGGCGCACCGTGATGAACCCTCTCG
CCGAAAAGCGCCGGGTGCTGATTCCGGACATGCTCGGCTTCGGCTTCACCGAACGTAAGCCGGACCAGCATTACGACGTG
GATGCCTGGGTCGTACAGGCGATCGGCCTGCTCGATGCGCTCGACCTGCCGCGCGCCGACATCGTCGGCAATTCCTTCGG
CGGTGCGCTGGCGCTCAAGCTCGCCATCCGCCACCCCAAGCGCATCCGCCGCATCGTGCTGATGGGAAGCGCGGGGCTGG
ATTTTCCGCTGACCGAGGGCCTGGACGCGGTGTGGGGCTACGAGCCCTCGCCGGAGAACATGCGCCGCATCCTCGACATC
TTCGCGTTCAACCGCAGCCTGATCACCGACGACCTCGCGCGGCTGCGCTACGAGGCGAGCATCCAGCCCGGCTTCCAGGA
AGCCTTCGCCGCGATGTTCCCGCCGCCGCGCCAGCGCTGGGTGAGCGCGCTCGCCAGCGCCGAGGACGACCTGCGCCGGA
TTCCGCACGAAACCCTGCTGGTGCATGGGCGCGAGGACAGGGTGATCCCGCTCGCCAGCTCGGTCACGCTGGCCGGGCTC
ATCCCGCGCGCGCAGCTCCATGTGTTCGGGCGTTGCGGCCACTGGACGCAGATCGAGCAGGCCGCGCGCTTCATCCGCCT
GGTCGCGGATTTTCTCGACGAGGCCGACAGCGCCGAGCCGCGCCCCCTCGACTGA

Upstream 100 bases:

>100_bases
TCTAATTAAAAATTTTTTTCTAATTTAAATCTCGAGATTTATAAGAGACGGACAGCGAGGCGATGCCCGAGCGGACGTCC
GGCCCCCAAGGAGTGCTTCA

Downstream 100 bases:

>100_bases
ACGACCCCGGCGCCGCCTGGCGCCCCTGCACAAACAAGGAAGGACGATGACGAAGGGAATCGAAGGACGCTGGGAGATCG
TCTCCTGGCGGCAGGAATAC

Product: 2-hydroxy-muconic semialdehyde hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: HMSH [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSQNNPELGSSIEIGSVRTNYHDLGKGFPVLMIHGSGPGVSGYVNWRTVMNPLAEKRRVLIPDMLGFGFTERKPDQHYDV
DAWVVQAIGLLDALDLPRADIVGNSFGGALALKLAIRHPKRIRRIVLMGSAGLDFPLTEGLDAVWGYEPSPENMRRILDI
FAFNRSLITDDLARLRYEASIQPGFQEAFAAMFPPPRQRWVSALASAEDDLRRIPHETLLVHGREDRVIPLASSVTLAGL
IPRAQLHVFGRCGHWTQIEQAARFIRLVADFLDEADSAEPRPLD

Sequences:

>Translated_284_residues
MSQNNPELGSSIEIGSVRTNYHDLGKGFPVLMIHGSGPGVSGYVNWRTVMNPLAEKRRVLIPDMLGFGFTERKPDQHYDV
DAWVVQAIGLLDALDLPRADIVGNSFGGALALKLAIRHPKRIRRIVLMGSAGLDFPLTEGLDAVWGYEPSPENMRRILDI
FAFNRSLITDDLARLRYEASIQPGFQEAFAAMFPPPRQRWVSALASAEDDLRRIPHETLLVHGREDRVIPLASSVTLAGL
IPRAQLHVFGRCGHWTQIEQAARFIRLVADFLDEADSAEPRPLD
>Mature_283_residues
SQNNPELGSSIEIGSVRTNYHDLGKGFPVLMIHGSGPGVSGYVNWRTVMNPLAEKRRVLIPDMLGFGFTERKPDQHYDVD
AWVVQAIGLLDALDLPRADIVGNSFGGALALKLAIRHPKRIRRIVLMGSAGLDFPLTEGLDAVWGYEPSPENMRRILDIF
AFNRSLITDDLARLRYEASIQPGFQEAFAAMFPPPRQRWVSALASAEDDLRRIPHETLLVHGREDRVIPLASSVTLAGLI
PRAQLHVFGRCGHWTQIEQAARFIRLVADFLDEADSAEPRPLD

Specific function: Conversion of 2-hydroxymuconic acid semialdehyde to 2- hydroxypent-2,4-dienoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=270, Percent_Identity=31.4814814814815, Blast_Score=132, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 31622; Mature: 31490

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNNPELGSSIEIGSVRTNYHDLGKGFPVLMIHGSGPGVSGYVNWRTVMNPLAEKRRVL
CCCCCCCCCCEEEECCEECCHHHHCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHCCEE
IPDMLGFGFTERKPDQHYDVDAWVVQAIGLLDALDLPRADIVGNSFGGALALKLAIRHPK
CCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHCHH
RIRRIVLMGSAGLDFPLTEGLDAVWGYEPSPENMRRILDIFAFNRSLITDDLARLRYEAS
HHEEEEEECCCCCCCCHHCCCHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCC
IQPGFQEAFAAMFPPPRQRWVSALASAEDDLRRIPHETLLVHGREDRVIPLASSVTLAGL
CCCCHHHHHHHHCCCHHHHHHHHHHHCHHHHHHCCCHHEEEECCCCCEEEEECCHHHHHH
IPRAQLHVFGRCGHWTQIEQAARFIRLVADFLDEADSAEPRPLD
CCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SQNNPELGSSIEIGSVRTNYHDLGKGFPVLMIHGSGPGVSGYVNWRTVMNPLAEKRRVL
CCCCCCCCCEEEECCEECCHHHHCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHCCEE
IPDMLGFGFTERKPDQHYDVDAWVVQAIGLLDALDLPRADIVGNSFGGALALKLAIRHPK
CCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHCHH
RIRRIVLMGSAGLDFPLTEGLDAVWGYEPSPENMRRILDIFAFNRSLITDDLARLRYEAS
HHEEEEEECCCCCCCCHHCCCHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCC
IQPGFQEAFAAMFPPPRQRWVSALASAEDDLRRIPHETLLVHGREDRVIPLASSVTLAGL
CCCCHHHHHHHHCCCHHHHHHHHHHHCHHHHHHCCCHHEEEECCCCCEEEEECCHHHHHH
IPRAQLHVFGRCGHWTQIEQAARFIRLVADFLDEADSAEPRPLD
CCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2194577 [H]